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The map label for this gene is eptA [H]

Identifier: 120610867

GI number: 120610867

Start: 2413148

End: 2414776

Strand: Reverse

Name: eptA [H]

Synonym: Aave_2193

Alternate gene names: 120610867

Gene position: 2414776-2413148 (Counterclockwise)

Preceding gene: 120610868

Following gene: 120610858

Centisome position: 45.11

GC content: 69.43

Gene sequence:

>1629_bases
ATGGCCGTGCTGCTGTTCGCCTGCCTCTGGATGGGCACGGCCTGCAACCTTCCGCTGTGGGGCGAGGTGCTGCGGCTGCC
GGGCGCGCACACGCTGCGCGGCGGGGTGTTTTCCCTGGCCTTCATGGTGGCGGTGTCGTTCGCCACGGCGGCCGTGCTCT
CGCTCTTCGCCTGGGGCAAGGCGCTCAAGCCCGTGCTTGCGCTGGCCGTGGTGGCGGCGGCGGCAGGCGCCTATTTCATG
GGCACTTACGGCATCGTGATCGATCCCGGAATGATCACCAACGTGGCCCAGACGGACGCGCGCGAAGCCGCGGACCTGTT
GACCTGGAAACTGCCGGTGGCGATGCTCGCCATGGCCGGCCCGCCGCTGGTGTGGCTGCTGCGCACCCGGGTGCGCCCGC
TGGGCTGGCGCCGGCGCGCGGTGCACCAGACGGCCTTCATGCTGGGTGCGCTGGCCGTCGCGGCGGCCTGCATTCTCGCG
GTGTTCCAGGACTTCTCTTCCACCATGCGCAACCACACGCGCATGCGCTACCTGGTCAATCCGCTCAACAGCGTCTATGC
CGTCGGCAGCCTGGCGGCCAGGCCTCTGCGCCATGACCGCGGCCCGCTGCAGCCGCTGGGCCGGGACGCGCGGCTGGGAG
CGAGCTATGCGGGCCAGGCGAAGCCGCCGCTGCTGGTGCTGGTGCTGGGCGAGACAGGGCGCAGCGGCAACTTCGGCCTG
AACGGCTACGCGCGCGATACGACGCCCGAGCTGTCGGCACGCAAGGACATCGCCACCGCCACCGATGCCTGGTCCTGCGG
CACCAGCACGGCCGCCTCCGTGCCCTGCATGTACGCGCACCTGGGCAAGGAAGCCTACGAGAAGCGCGGCGCGGACTACG
AAACCCTGATGGACGTGCTGCAGCATGCCGGCCTGGCGGTGCTGTGGGTGGACAACCAGTCCGGCTGCAAGGGCGTGTGC
GACCGCGTGTCCACCGCCAGCACGTCCGCCACGCCCAACGACCCGACCTGGTGCGCGGACGGCAAGGAATGCCTGGACGA
CGTCATGCTGAAGGGCCTGGACGCGCGCATCGCCGCCCTGCCCGCCGAGCAGCGTGCGCGCGGCGTGGTGGTGGTGCTGC
ACCAGATGGGCAGCCACGGGCCGGCCTACGCCAGGCGATCGCTGCCTTCGCAGAAGAGATTCCAGCCCGAGTGCAAGACC
TCCGCATTGCAGGAATGCACGCGCGAGCAGGTGGTGAATGCCTACGACAACAGCATCGTCTCCACCGACCATTTCCTGAA
CAGCACCATCGGCTGGCTGGAGGCGCGCTCGGCCAGCGCCGAGACCGCCATGGTGTACGTGGCCGACCACGGCGAATCCC
TGGGCGAGAACGGCATCTACCTGCACGGCATGCCCTACTCCATCGCGCCCGATGTGCAAAAGCACGTGCCGTGGATCACC
TGGCTGTCGCCGGCCATGCAGCAGCGCACGGGCATCGCGACCGGCTGTCTGCAGAAGGACCTGCGCAGCCGCCGCATCAC
GCACGACAACTATTTCCATTCGGTGCTCGGGCTGATGGACGTGCAGACGGGCGCCTACCAGCCGGCGAAGGACATGTACG
CGGCTTGCCGCGTGGCGGCACGGGGCTGA

Upstream 100 bases:

>100_bases
CACTGCCGCCATGTTCCCGTCGTCCGCCGTCCCGCTCCGCCCCCCTGCCCTTCCTTCCAGCCGTTTTCCGGCCCGTGCGC
CCTCCGGGGGGCTGCATCCC

Downstream 100 bases:

>100_bases
GAAGCGGCCCCCGTGCCCGCGCGCTGCCACGGGGCAGCGCGGCAATCCTTCTTCAGTCCAGCTGCCCCTGGCAAAGGTAC
TTGGTGTGCAGGTAGTCGTC

Product: sulfatase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MAVLLFACLWMGTACNLPLWGEVLRLPGAHTLRGGVFSLAFMVAVSFATAAVLSLFAWGKALKPVLALAVVAAAAGAYFM
GTYGIVIDPGMITNVAQTDAREAADLLTWKLPVAMLAMAGPPLVWLLRTRVRPLGWRRRAVHQTAFMLGALAVAAACILA
VFQDFSSTMRNHTRMRYLVNPLNSVYAVGSLAARPLRHDRGPLQPLGRDARLGASYAGQAKPPLLVLVLGETGRSGNFGL
NGYARDTTPELSARKDIATATDAWSCGTSTAASVPCMYAHLGKEAYEKRGADYETLMDVLQHAGLAVLWVDNQSGCKGVC
DRVSTASTSATPNDPTWCADGKECLDDVMLKGLDARIAALPAEQRARGVVVVLHQMGSHGPAYARRSLPSQKRFQPECKT
SALQECTREQVVNAYDNSIVSTDHFLNSTIGWLEARSASAETAMVYVADHGESLGENGIYLHGMPYSIAPDVQKHVPWIT
WLSPAMQQRTGIATGCLQKDLRSRRITHDNYFHSVLGLMDVQTGAYQPAKDMYAACRVAARG

Sequences:

>Translated_542_residues
MAVLLFACLWMGTACNLPLWGEVLRLPGAHTLRGGVFSLAFMVAVSFATAAVLSLFAWGKALKPVLALAVVAAAAGAYFM
GTYGIVIDPGMITNVAQTDAREAADLLTWKLPVAMLAMAGPPLVWLLRTRVRPLGWRRRAVHQTAFMLGALAVAAACILA
VFQDFSSTMRNHTRMRYLVNPLNSVYAVGSLAARPLRHDRGPLQPLGRDARLGASYAGQAKPPLLVLVLGETGRSGNFGL
NGYARDTTPELSARKDIATATDAWSCGTSTAASVPCMYAHLGKEAYEKRGADYETLMDVLQHAGLAVLWVDNQSGCKGVC
DRVSTASTSATPNDPTWCADGKECLDDVMLKGLDARIAALPAEQRARGVVVVLHQMGSHGPAYARRSLPSQKRFQPECKT
SALQECTREQVVNAYDNSIVSTDHFLNSTIGWLEARSASAETAMVYVADHGESLGENGIYLHGMPYSIAPDVQKHVPWIT
WLSPAMQQRTGIATGCLQKDLRSRRITHDNYFHSVLGLMDVQTGAYQPAKDMYAACRVAARG
>Mature_541_residues
AVLLFACLWMGTACNLPLWGEVLRLPGAHTLRGGVFSLAFMVAVSFATAAVLSLFAWGKALKPVLALAVVAAAAGAYFMG
TYGIVIDPGMITNVAQTDAREAADLLTWKLPVAMLAMAGPPLVWLLRTRVRPLGWRRRAVHQTAFMLGALAVAAACILAV
FQDFSSTMRNHTRMRYLVNPLNSVYAVGSLAARPLRHDRGPLQPLGRDARLGASYAGQAKPPLLVLVLGETGRSGNFGLN
GYARDTTPELSARKDIATATDAWSCGTSTAASVPCMYAHLGKEAYEKRGADYETLMDVLQHAGLAVLWVDNQSGCKGVCD
RVSTASTSATPNDPTWCADGKECLDDVMLKGLDARIAALPAEQRARGVVVVLHQMGSHGPAYARRSLPSQKRFQPECKTS
ALQECTREQVVNAYDNSIVSTDHFLNSTIGWLEARSASAETAMVYVADHGESLGENGIYLHGMPYSIAPDVQKHVPWITW
LSPAMQQRTGIATGCLQKDLRSRRITHDNYFHSVLGLMDVQTGAYQPAKDMYAACRVAARG

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=537, Percent_Identity=36.8715083798883, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI87082286, Length=441, Percent_Identity=25.6235827664399, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI87082223, Length=268, Percent_Identity=28.3582089552239, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 58452; Mature: 58321

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLLFACLWMGTACNLPLWGEVLRLPGAHTLRGGVFSLAFMVAVSFATAAVLSLFAWGK
CHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKPVLALAVVAAAAGAYFMGTYGIVIDPGMITNVAQTDAREAADLLTWKLPVAMLAMAG
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PPLVWLLRTRVRPLGWRRRAVHQTAFMLGALAVAAACILAVFQDFSSTMRNHTRMRYLVN
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLNSVYAVGSLAARPLRHDRGPLQPLGRDARLGASYAGQAKPPLLVLVLGETGRSGNFGL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCC
NGYARDTTPELSARKDIATATDAWSCGTSTAASVPCMYAHLGKEAYEKRGADYETLMDVL
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCHHHHHHHH
QHAGLAVLWVDNQSGCKGVCDRVSTASTSATPNDPTWCADGKECLDDVMLKGLDARIAAL
HHCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHC
PAEQRARGVVVVLHQMGSHGPAYARRSLPSQKRFQPECKTSALQECTREQVVNAYDNSIV
CHHHHCCCEEEEEECCCCCCHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCC
STDHFLNSTIGWLEARSASAETAMVYVADHGESLGENGIYLHGMPYSIAPDVQKHVPWIT
CHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCHHHHCCHHH
WLSPAMQQRTGIATGCLQKDLRSRRITHDNYFHSVLGLMDVQTGAYQPAKDMYAACRVAA
HHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
RG
CC
>Mature Secondary Structure 
AVLLFACLWMGTACNLPLWGEVLRLPGAHTLRGGVFSLAFMVAVSFATAAVLSLFAWGK
HHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8282725 [H]