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The map label for this gene is trmJ [H]

Identifier: 120610753

GI number: 120610753

Start: 2277036

End: 2277869

Strand: Direct

Name: trmJ [H]

Synonym: Aave_2075

Alternate gene names: 120610753

Gene position: 2277036-2277869 (Clockwise)

Preceding gene: 120610752

Following gene: 120610754

Centisome position: 42.54

GC content: 71.1

Gene sequence:

>834_bases
ATGAAAACCCGCTTCATCCTCATCCAGACCAGCCATGCCGGCAACGTCGGCGCCGCCGCCCGCGCCATGAAGACCATGGG
CTTCGGGGATCTGGTCCTGGTGGCCCCGCGCTGGTCCAACGTGCTGCGGCGCGAGGAAACCATCCAGCGCGCGAGCGGCG
CGCTGGACGTGCTGGAGAACGCCCGCATCGTCGCCACGCTGGACGAGGCCCTGGACGGCATCAGCCACCTGTGCGCCACC
GCGATGACGCCGCGTGATTTCGGCCCGCCCACGCGATCGCCGCGCGAGCATTTCGAGTGGCTGGCGCGCCGGCAGTCCGC
CGATGCGGCCCATCCCCTGGCCGAACTGGCCCTGGAAGGGGGCATCGCCCCCGTGCCGCCCGCGGACGCCGATCCGGTCT
CCGCGCCAGCGGCTGCGGCAGGGGAGGCGGGCGTGGCCTTCCTGTTCGGCTCCGAGCGTTTCGGCATGGCGAACGAGGAC
GTGTACCGCTGCCATGTCGCGCTTTCCATTCCCACGAATCCCGGCTTCGGCTCGCTGAATCTCGGGGCGGCCATCCAGGT
GATCGCCTACGAGTGGCGGCTCGCGCTCGGCGGCTTCCCCGTGCGGGACGCCGCGCCCGCGTCCGTGCCGGCCGACGCCG
CCCAGGTGGCCGGCATGCTCGCGCACTGGGAGCGCGCGCTGGTGGATGTCGGCTTCCTCGACCCGGCCGCGCCCAAGAAG
CTCATGCCGCGGCTCAACCAGCTTTTCAACCGCGCGCAACCCACCCCCGAGGAAATCCACATCCTGCGCGGTGTCGCAAA
AGCCATGTCGGAAGCGGCACGGGCGAAGCGATAG

Upstream 100 bases:

>100_bases
AGGCCGGCGGGAGCGGGCGGGAGCGGGCGGGAGCGGGCGGGAGCGGGCGGGAGCGGCGCAGCGGCGACAATCGCGCGTCC
TTTCCGCTGTGCAGCTTTCC

Downstream 100 bases:

>100_bases
ACTGCAAGGCCGATTCCCGCCCCGCGTCCCTTCCACGAGCCCCCTCCCCATGTTCGACCGCCTGCGCTCCGACATCCAGT
GCATCCTCGACCGCGACCCC

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKTRFILIQTSHAGNVGAAARAMKTMGFGDLVLVAPRWSNVLRREETIQRASGALDVLENARIVATLDEALDGISHLCAT
AMTPRDFGPPTRSPREHFEWLARRQSADAAHPLAELALEGGIAPVPPADADPVSAPAAAAGEAGVAFLFGSERFGMANED
VYRCHVALSIPTNPGFGSLNLGAAIQVIAYEWRLALGGFPVRDAAPASVPADAAQVAGMLAHWERALVDVGFLDPAAPKK
LMPRLNQLFNRAQPTPEEIHILRGVAKAMSEAARAKR

Sequences:

>Translated_277_residues
MKTRFILIQTSHAGNVGAAARAMKTMGFGDLVLVAPRWSNVLRREETIQRASGALDVLENARIVATLDEALDGISHLCAT
AMTPRDFGPPTRSPREHFEWLARRQSADAAHPLAELALEGGIAPVPPADADPVSAPAAAAGEAGVAFLFGSERFGMANED
VYRCHVALSIPTNPGFGSLNLGAAIQVIAYEWRLALGGFPVRDAAPASVPADAAQVAGMLAHWERALVDVGFLDPAAPKK
LMPRLNQLFNRAQPTPEEIHILRGVAKAMSEAARAKR
>Mature_277_residues
MKTRFILIQTSHAGNVGAAARAMKTMGFGDLVLVAPRWSNVLRREETIQRASGALDVLENARIVATLDEALDGISHLCAT
AMTPRDFGPPTRSPREHFEWLARRQSADAAHPLAELALEGGIAPVPPADADPVSAPAAAAGEAGVAFLFGSERFGMANED
VYRCHVALSIPTNPGFGSLNLGAAIQVIAYEWRLALGGFPVRDAAPASVPADAAQVAGMLAHWERALVDVGFLDPAAPKK
LMPRLNQLFNRAQPTPEEIHILRGVAKAMSEAARAKR

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=280, Percent_Identity=35, Blast_Score=150, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29462; Mature: 29462

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRFILIQTSHAGNVGAAARAMKTMGFGDLVLVAPRWSNVLRREETIQRASGALDVLEN
CCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHC
ARIVATLDEALDGISHLCATAMTPRDFGPPTRSPREHFEWLARRQSADAAHPLAELALEG
CEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHC
GIAPVPPADADPVSAPAAAAGEAGVAFLFGSERFGMANEDVYRCHVALSIPTNPGFGSLN
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCC
LGAAIQVIAYEWRLALGGFPVRDAAPASVPADAAQVAGMLAHWERALVDVGFLDPAAPKK
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LMPRLNQLFNRAQPTPEEIHILRGVAKAMSEAARAKR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTRFILIQTSHAGNVGAAARAMKTMGFGDLVLVAPRWSNVLRREETIQRASGALDVLEN
CCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHC
ARIVATLDEALDGISHLCATAMTPRDFGPPTRSPREHFEWLARRQSADAAHPLAELALEG
CEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHC
GIAPVPPADADPVSAPAAAAGEAGVAFLFGSERFGMANEDVYRCHVALSIPTNPGFGSLN
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCC
LGAAIQVIAYEWRLALGGFPVRDAAPASVPADAAQVAGMLAHWERALVDVGFLDPAAPKK
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LMPRLNQLFNRAQPTPEEIHILRGVAKAMSEAARAKR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA