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The map label for this gene is oprM [H]

Identifier: 120610720

GI number: 120610720

Start: 2227108

End: 2228643

Strand: Direct

Name: oprM [H]

Synonym: Aave_2041

Alternate gene names: 120610720

Gene position: 2227108-2228643 (Clockwise)

Preceding gene: 120610719

Following gene: 120610721

Centisome position: 41.61

GC content: 77.93

Gene sequence:

>1536_bases
ATGACCTTCGATCCGTCCGCCCCCCGGCCCGCCCCGGCCGCCCGCCCTGCGGCCCGCGCGCCGTCCGCCCCGCGCATGCT
GCGCGCCTGCGCCGCGGCGCTGCTGCCGGCCTGGCTGGCCGCCTGCTCGGTGGCGCCTGCCTACCAGCCCCCGGCGCTCG
CCACCCCCATGCCCACCGCCTTCAAGGAAGCGGGCGCGCTCTGGCAGCCGGCCGCGCCCGCCGACGCCGCCGACCGCGGC
GACTGGTGGACGCTGTTCGGCGACGAGGAATTGAACCGCCTCGCCGCCCGGGTGCAGGTATCGAACCAGAACATCGCCGC
CGCCGTGGCTGCCTATGCCCAGGCCCAGGCGCTGGTGCGCGAGCAGCGCGCGGGCCTGCTGCCGGGCGTGAGCCTGTCGG
CCGGCGAAACCCGTGCGGGCGGCGAGACCCGTGCGGGCGGCGAGGGCTCCGCGCGCCGCGGCACCACGGCCCAGGCGGCG
CTGGGCGCGAGCTGGGCGCCGGACGTGTGGGGGCGCCTGGGCAGCGCCGTGGACAGCGCCCGGGCCAACGCCCAGGCCAG
CGAGGCGGACCTGGCCGCGGCGCGGCTCTCCGCCGTGGGCTCGCTCGTGGCCGCCTATTTCCAGCTGCGCGAGGCCGACG
CCGAGGCCGGGCTGCTGCGCGACACGGTGCAGGCCTACGAGCGCGCACTGCGCATCGCCCGGAACCGCTACGACGCCGGC
GTGGCCGCGCAATCGGACGTACTGCAGGCGCGCACCCAGCTGGCCAACGCCCGCGCCGATCTCGCCACCGCGCAGCGCAA
CCGCGCCGGCTACGAGCACGCCATCGCGGTGCTGGCGGGCCAGCCGCCGGCGGCGTTCAGCCTGCCTGCCGGCACCTGGG
TGGCACGCGTTCCCGAGGTGCCCGCGGGGCTGCCCTCGACCCTGCTGGAGCGCCGCCCGGACATCGCCGCGGCCGAGCGC
GCCGTGGCCGCCGCCAACGCGCAGATCGGCGTGCAGCGTTCCGCCTACTACCCCAGCTTCAGCCTCACGGCCTCCCTGGG
GCGCAGCGGCACCGGCCTGGGCGACCTGTTCAGCGCCTCGGGCGCGCTGTGGTCGCTGGGCCTGTCGGCCGCCCAGACGG
TGTTCGACGGCGGCGCCATCGCCGCGCGCGTGGAGCAGGCGCGCGCGGCCCGCGACGCGCGCGTGGCCAACTACCGCCAG
ACGGTGCTGGGCGCCTTCCAGGCGGTGGAAGACCAGCTCACGGCGGTGCGCACCCTGCAGGCGCAGGAGCCCCTGCGGCA
GGAGGCCGTCTCGGCCGCCCAGCGCGCGGAGGAGCAGATGCTCAACCGCTACCGTGCCGGCCAGGTGGGCTACACCGACG
TGGTCACCGCCCAGGTGGCGGCGCTGTCCGCGCGGCGCGCGCTGCTGCAGCTGCAGGTGAACCGCCAGCTCGCGGTGGCA
TCGCTCGTCCAGGCGCTGGGCGGCGGCTGGCAGGCCCCCTGGATGGACGGCGCCGCCGCGGCCACGGCCGGGCAGGGTGC
TGCCGAGCCCCGATAA

Upstream 100 bases:

>100_bases
TGGTCTATGTGCTGCTCGACCGGCTCCGCCGCCCCTCCGGCAATGAACGACACCTCAGCCGCGCCGCCGACACGGCACCG
GCCGTGCCTGCGCGACACCC

Downstream 100 bases:

>100_bases
TCCGCGGCTGCTTTCCCCTCCCAACGAAAAAGGATTTCCGTGGATTTCATCTTGCTGGCCAAGGCCGCCGTCATGGGCGT
GGTCGAGGGCCTGACCGAGT

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MTFDPSAPRPAPAARPAARAPSAPRMLRACAAALLPAWLAACSVAPAYQPPALATPMPTAFKEAGALWQPAAPADAADRG
DWWTLFGDEELNRLAARVQVSNQNIAAAVAAYAQAQALVREQRAGLLPGVSLSAGETRAGGETRAGGEGSARRGTTAQAA
LGASWAPDVWGRLGSAVDSARANAQASEADLAAARLSAVGSLVAAYFQLREADAEAGLLRDTVQAYERALRIARNRYDAG
VAAQSDVLQARTQLANARADLATAQRNRAGYEHAIAVLAGQPPAAFSLPAGTWVARVPEVPAGLPSTLLERRPDIAAAER
AVAAANAQIGVQRSAYYPSFSLTASLGRSGTGLGDLFSASGALWSLGLSAAQTVFDGGAIAARVEQARAARDARVANYRQ
TVLGAFQAVEDQLTAVRTLQAQEPLRQEAVSAAQRAEEQMLNRYRAGQVGYTDVVTAQVAALSARRALLQLQVNRQLAVA
SLVQALGGGWQAPWMDGAAAATAGQGAAEPR

Sequences:

>Translated_511_residues
MTFDPSAPRPAPAARPAARAPSAPRMLRACAAALLPAWLAACSVAPAYQPPALATPMPTAFKEAGALWQPAAPADAADRG
DWWTLFGDEELNRLAARVQVSNQNIAAAVAAYAQAQALVREQRAGLLPGVSLSAGETRAGGETRAGGEGSARRGTTAQAA
LGASWAPDVWGRLGSAVDSARANAQASEADLAAARLSAVGSLVAAYFQLREADAEAGLLRDTVQAYERALRIARNRYDAG
VAAQSDVLQARTQLANARADLATAQRNRAGYEHAIAVLAGQPPAAFSLPAGTWVARVPEVPAGLPSTLLERRPDIAAAER
AVAAANAQIGVQRSAYYPSFSLTASLGRSGTGLGDLFSASGALWSLGLSAAQTVFDGGAIAARVEQARAARDARVANYRQ
TVLGAFQAVEDQLTAVRTLQAQEPLRQEAVSAAQRAEEQMLNRYRAGQVGYTDVVTAQVAALSARRALLQLQVNRQLAVA
SLVQALGGGWQAPWMDGAAAATAGQGAAEPR
>Mature_510_residues
TFDPSAPRPAPAARPAARAPSAPRMLRACAAALLPAWLAACSVAPAYQPPALATPMPTAFKEAGALWQPAAPADAADRGD
WWTLFGDEELNRLAARVQVSNQNIAAAVAAYAQAQALVREQRAGLLPGVSLSAGETRAGGETRAGGEGSARRGTTAQAAL
GASWAPDVWGRLGSAVDSARANAQASEADLAAARLSAVGSLVAAYFQLREADAEAGLLRDTVQAYERALRIARNRYDAGV
AAQSDVLQARTQLANARADLATAQRNRAGYEHAIAVLAGQPPAAFSLPAGTWVARVPEVPAGLPSTLLERRPDIAAAERA
VAAANAQIGVQRSAYYPSFSLTASLGRSGTGLGDLFSASGALWSLGLSAAQTVFDGGAIAARVEQARAARDARVANYRQT
VLGAFQAVEDQLTAVRTLQAQEPLRQEAVSAAQRAEEQMLNRYRAGQVGYTDVVTAQVAALSARRALLQLQVNRQLAVAS
LVQALGGGWQAPWMDGAAAATAGQGAAEPR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=454, Percent_Identity=28.8546255506608, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI2367348, Length=342, Percent_Identity=27.7777777777778, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52878; Mature: 52746

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFDPSAPRPAPAARPAARAPSAPRMLRACAAALLPAWLAACSVAPAYQPPALATPMPTA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
FKEAGALWQPAAPADAADRGDWWTLFGDEELNRLAARVQVSNQNIAAAVAAYAQAQALVR
HHHCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
EQRAGLLPGVSLSAGETRAGGETRAGGEGSARRGTTAQAALGASWAPDVWGRLGSAVDSA
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHH
RANAQASEADLAAARLSAVGSLVAAYFQLREADAEAGLLRDTVQAYERALRIARNRYDAG
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VAAQSDVLQARTQLANARADLATAQRNRAGYEHAIAVLAGQPPAAFSLPAGTWVARVPEV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEECCCCCCEECCCCCHHHHCCCC
PAGLPSTLLERRPDIAAAERAVAAANAQIGVQRSAYYPSFSLTASLGRSGTGLGDLFSAS
CCCCCHHHHHCCCCHHHHHHHHHHHCCHHCCCHHCCCCCHHHHHHCCCCCCCHHHHHHCC
GALWSLGLSAAQTVFDGGAIAARVEQARAARDARVANYRQTVLGAFQAVEDQLTAVRTLQ
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQEPLRQEAVSAAQRAEEQMLNRYRAGQVGYTDVVTAQVAALSARRALLQLQVNRQLAVA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLVQALGGGWQAPWMDGAAAATAGQGAAEPR
HHHHHHCCCCCCCCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure 
TFDPSAPRPAPAARPAARAPSAPRMLRACAAALLPAWLAACSVAPAYQPPALATPMPTA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
FKEAGALWQPAAPADAADRGDWWTLFGDEELNRLAARVQVSNQNIAAAVAAYAQAQALVR
HHHCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
EQRAGLLPGVSLSAGETRAGGETRAGGEGSARRGTTAQAALGASWAPDVWGRLGSAVDSA
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHH
RANAQASEADLAAARLSAVGSLVAAYFQLREADAEAGLLRDTVQAYERALRIARNRYDAG
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VAAQSDVLQARTQLANARADLATAQRNRAGYEHAIAVLAGQPPAAFSLPAGTWVARVPEV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEECCCCCCEECCCCCHHHHCCCC
PAGLPSTLLERRPDIAAAERAVAAANAQIGVQRSAYYPSFSLTASLGRSGTGLGDLFSAS
CCCCCHHHHHCCCCHHHHHHHHHHHCCHHCCCHHCCCCCHHHHHHCCCCCCCHHHHHHCC
GALWSLGLSAAQTVFDGGAIAARVEQARAARDARVANYRQTVLGAFQAVEDQLTAVRTLQ
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQEPLRQEAVSAAQRAEEQMLNRYRAGQVGYTDVVTAQVAALSARRALLQLQVNRQLAVA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLVQALGGGWQAPWMDGAAAATAGQGAAEPR
HHHHHHCCCCCCCCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]