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The map label for this gene is nth [H]

Identifier: 120610247

GI number: 120610247

Start: 1728562

End: 1729242

Strand: Reverse

Name: nth [H]

Synonym: Aave_1562

Alternate gene names: 120610247

Gene position: 1729242-1728562 (Counterclockwise)

Preceding gene: 120610252

Following gene: 120610244

Centisome position: 32.31

GC content: 67.69

Gene sequence:

>681_bases
ATGCACGGGCGAACCGCATCGACAACGACGCCCCACACCACCATGAAGACCGCGCAGATCGAACCCTTCTTCGCCGCGCT
GAAGGCCGCCAATCCCCAACCCAACACCGAACTGGAATACACCACCGTCTTCGAGCTGCTGGCCGCCGTGCTGTTGTCGG
CCCAGGCCACCGACGTGGGCGTGAACAAGGCCACGCGCAAGCTGTTCCCGGTGGCGGGCACGCCGCAGGCCATCCTCGAC
CTGGGGCTGGAAGGGCTGGAGGGCTACATCAAGACCATTGGCCTGTACCGCAGCAAGGCCCGCCACCTGATGGAGACGTG
CCGCATCCTGGTGGAGCGCCACGGCGGCACCGTGCCGCGCACGCGCGAGGAACTGGAAGCCCTGCCCGGCGTGGGCCGCA
AGACCGCCAACGTGGTGCTGAACGTGGCCTTCGGCCAGCCCACCATGGCGGTGGACACGCACATCTTCCGCGTGAGCAAC
CGCACCGGCCTCGCCCCCGGCAAGAACCCGCTGGCCGTGGAAATGCAGCTGCTCAAACGCGTGCCCGCCGAATATGCCGT
GGATTCGCACCACTGGCTCATCCTGCTGGGCCGCTACGTCTGCCAGGCGAGAAAGCCGCGCTGCTGGGAATGCGTGGTGG
CGCCGTGGTGCGATTACCAGCCGAAGACGCCGGCGCCCTGA

Upstream 100 bases:

>100_bases
CGGTCTGCGAATTGCATGGCGTGTTCACGGTGTGTTGGCGAAAGACCCCATTGTCGCCAATGGCCGCGCATGTTTCATGC
GTACGGTGCGCTGCGGGACA

Downstream 100 bases:

>100_bases
GGCGGGGCCCTGCTTCCGCTCCTTTCTTCTTGCCGGGCGCTGCGAACCGCGAGTGACGGGCTGGTCCGCCTGGGGTTCGC
GCAGGTGCAAGTGGTTGAGG

Product: DNA-(apurinic or apyrimidinic site) lyase / endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MHGRTASTTTPHTTMKTAQIEPFFAALKAANPQPNTELEYTTVFELLAAVLLSAQATDVGVNKATRKLFPVAGTPQAILD
LGLEGLEGYIKTIGLYRSKARHLMETCRILVERHGGTVPRTREELEALPGVGRKTANVVLNVAFGQPTMAVDTHIFRVSN
RTGLAPGKNPLAVEMQLLKRVPAEYAVDSHHWLILLGRYVCQARKPRCWECVVAPWCDYQPKTPAP

Sequences:

>Translated_226_residues
MHGRTASTTTPHTTMKTAQIEPFFAALKAANPQPNTELEYTTVFELLAAVLLSAQATDVGVNKATRKLFPVAGTPQAILD
LGLEGLEGYIKTIGLYRSKARHLMETCRILVERHGGTVPRTREELEALPGVGRKTANVVLNVAFGQPTMAVDTHIFRVSN
RTGLAPGKNPLAVEMQLLKRVPAEYAVDSHHWLILLGRYVCQARKPRCWECVVAPWCDYQPKTPAP
>Mature_226_residues
MHGRTASTTTPHTTMKTAQIEPFFAALKAANPQPNTELEYTTVFELLAAVLLSAQATDVGVNKATRKLFPVAGTPQAILD
LGLEGLEGYIKTIGLYRSKARHLMETCRILVERHGGTVPRTREELEALPGVGRKTANVVLNVAFGQPTMAVDTHIFRVSN
RTGLAPGKNPLAVEMQLLKRVPAEYAVDSHHWLILLGRYVCQARKPRCWECVVAPWCDYQPKTPAP

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.638418079096, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI115298654, Length=151, Percent_Identity=36.4238410596026, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI115298652, Length=151, Percent_Identity=36.4238410596026, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI115298650, Length=151, Percent_Identity=36.4238410596026, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI190358497, Length=151, Percent_Identity=36.4238410596026, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI6912520, Length=151, Percent_Identity=36.4238410596026, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI115298648, Length=151, Percent_Identity=36.4238410596026, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=64.4230769230769, Blast_Score=276, Evalue=8e-76,
Organism=Escherichia coli, GI1789331, Length=121, Percent_Identity=32.2314049586777, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=33.1428571428571, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=32.2222222222222, Blast_Score=105, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24865; Mature: 24865

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGRTASTTTPHTTMKTAQIEPFFAALKAANPQPNTELEYTTVFELLAAVLLSAQATDVG
CCCCCCCCCCCCCEEECCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
VNKATRKLFPVAGTPQAILDLGLEGLEGYIKTIGLYRSKARHLMETCRILVERHGGTVPR
CCHHHHHEECCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TREELEALPGVGRKTANVVLNVAFGQPTMAVDTHIFRVSNRTGLAPGKNPLAVEMQLLKR
CHHHHHHCCCCCCCCEEEEEEEECCCCCEEHHHEEEEECCCCCCCCCCCCHHHHHHHHHH
VPAEYAVDSHHWLILLGRYVCQARKPRCWECVVAPWCDYQPKTPAP
CCHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MHGRTASTTTPHTTMKTAQIEPFFAALKAANPQPNTELEYTTVFELLAAVLLSAQATDVG
CCCCCCCCCCCCCEEECCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
VNKATRKLFPVAGTPQAILDLGLEGLEGYIKTIGLYRSKARHLMETCRILVERHGGTVPR
CCHHHHHEECCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TREELEALPGVGRKTANVVLNVAFGQPTMAVDTHIFRVSNRTGLAPGKNPLAVEMQLLKR
CHHHHHHCCCCCCCCEEEEEEEECCCCCEEHHHEEEEECCCCCCCCCCCCHHHHHHHHHH
VPAEYAVDSHHWLILLGRYVCQARKPRCWECVVAPWCDYQPKTPAP
CCHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]