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The map label for this gene is kgtP [H]

Identifier: 120610244

GI number: 120610244

Start: 1724996

End: 1726324

Strand: Reverse

Name: kgtP [H]

Synonym: Aave_1559

Alternate gene names: 120610244

Gene position: 1726324-1724996 (Counterclockwise)

Preceding gene: 120610247

Following gene: 120610242

Centisome position: 32.25

GC content: 67.27

Gene sequence:

>1329_bases
ATGAGCACGCACACCCCTTCCGCGGCCGGCACGCCGCACCCCGAACCCCGCGCCTACACGGCCGAAGAGAAGCGCCACCG
GGTCTTCTCGATCATGGCGGCCTCGTCGGGCAACCTCGTGGAATGGTTCGATTTCTACGTGTATGCGTTCGCGGCGCTGT
ATTTCGCGCCGGCGTTCTTCCCGAAATCGGACCCCACGGCCCAGCTGCTGAACACGGCGGGCGTGTTCGCCGCGGGCTTC
CTCATGCGGCCCATCGGCGGCTGGCTGTTCGGCCGCATCGCGGACCGCTACGGCCGCAAGACCTCGATGCTGATCTCGGT
CACCATGATGTGCGCGGGCTCGCTGGTGATCGCGTGCCTGCCCACCTATGCGGCCATCGGGGCCTGGGCGCCCTTCCTGC
TGCTGGTGTGCCGGCTGTTCCAGGGCCTGTCGGTGGGTGGCGAATACGGCACCACGGCCACCTACATGAGCGAGGTGGCG
CTGCGCGGCCAGCGCGGCTTCTTCTCGTCGTTCCAGTACGTGACGCTGATCGGCGGGCAACTGCTGGCGGTGCTGGTGAT
CGTGATCCTGGAGACGCTGCTGTCCGAGGCCGAGCTCAAGGCCTGGGGCTGGCGCATCCCGTTCGTGATCGGCGCCATCT
CGGCGGTGGTCGCGCTGATGCTGCGCCGCACGCTGCACGAGACGCAGAGCGACGAGAACAGGAAGAAGGACGACGCGGGC
ACCATCGGCGGCCTGTTCCGCCACCACAAGGCGGCCTTCTTCACGGTGCTGGGCTACACGGCCGGCGGCTCGCTGATCTT
CTACACCTTCACGACCTACATGCAGAAGTACCTCGTGAACACGGTGGGCCTGCCGATCAAGACGACCAGCTACGTGATGA
CCGGCGCGCTGTTCCTCTACATGTGCATGCAGCCCCTCTTCGGCGCGCTGTCGGACCGCATCGGGCGGCGCAACAACATG
CTGCTGTTCGGCGCGCTGGGCGCCATCGGCACGGTGCCGATCCTCACCACGCTGCAGCACGTGACCAGCCCGGTGGCGGC
CTTCGTGCTGATCGTGGTGGCGCTGGCCATCGTGAGCTTCTACACCTCGATCAGCGGCATCGTGAAGGCGGAGATGTTCC
CGCCCGAGGTGCGCGCGCTGGGCGTGGGCCTGGCCTACGCGGTGGCCAACGCGATCTTCGGCGGCTCGGCGGAGTACGTC
GCGCTGGGGCTGAAGACGCTGGGGCACGAGTCGGCGTTCTTCTGGTACGTGACGGCGATGATGGTCGTGGCCTTCCTGGT
GAGCCTGCGGCTGCCGCGGCAGGCGAGCTATCTGCACCACGATCATTGA

Upstream 100 bases:

>100_bases
GCCTTCCGCAGCGCCCGCACAATACCTGCCGGCCAGAAACAACATCACACACTCTGCGTGCCGGCCCTGCGGCGCGCACG
GCCAGAAAGGCATCTTCTCC

Downstream 100 bases:

>100_bases
TCCCATCGCCGGGGGCGAGGCGCGCGACGGCCCCCGCATTGGCACCGTGAGCGCGGATCGGACTGATCCGCGCCAAAGTC
CTGAAAACCCACTCGCCAAT

Product: major facilitator superfamily metabolite/H(+) symporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSTHTPSAAGTPHPEPRAYTAEEKRHRVFSIMAASSGNLVEWFDFYVYAFAALYFAPAFFPKSDPTAQLLNTAGVFAAGF
LMRPIGGWLFGRIADRYGRKTSMLISVTMMCAGSLVIACLPTYAAIGAWAPFLLLVCRLFQGLSVGGEYGTTATYMSEVA
LRGQRGFFSSFQYVTLIGGQLLAVLVIVILETLLSEAELKAWGWRIPFVIGAISAVVALMLRRTLHETQSDENRKKDDAG
TIGGLFRHHKAAFFTVLGYTAGGSLIFYTFTTYMQKYLVNTVGLPIKTTSYVMTGALFLYMCMQPLFGALSDRIGRRNNM
LLFGALGAIGTVPILTTLQHVTSPVAAFVLIVVALAIVSFYTSISGIVKAEMFPPEVRALGVGLAYAVANAIFGGSAEYV
ALGLKTLGHESAFFWYVTAMMVVAFLVSLRLPRQASYLHHDH

Sequences:

>Translated_442_residues
MSTHTPSAAGTPHPEPRAYTAEEKRHRVFSIMAASSGNLVEWFDFYVYAFAALYFAPAFFPKSDPTAQLLNTAGVFAAGF
LMRPIGGWLFGRIADRYGRKTSMLISVTMMCAGSLVIACLPTYAAIGAWAPFLLLVCRLFQGLSVGGEYGTTATYMSEVA
LRGQRGFFSSFQYVTLIGGQLLAVLVIVILETLLSEAELKAWGWRIPFVIGAISAVVALMLRRTLHETQSDENRKKDDAG
TIGGLFRHHKAAFFTVLGYTAGGSLIFYTFTTYMQKYLVNTVGLPIKTTSYVMTGALFLYMCMQPLFGALSDRIGRRNNM
LLFGALGAIGTVPILTTLQHVTSPVAAFVLIVVALAIVSFYTSISGIVKAEMFPPEVRALGVGLAYAVANAIFGGSAEYV
ALGLKTLGHESAFFWYVTAMMVVAFLVSLRLPRQASYLHHDH
>Mature_441_residues
STHTPSAAGTPHPEPRAYTAEEKRHRVFSIMAASSGNLVEWFDFYVYAFAALYFAPAFFPKSDPTAQLLNTAGVFAAGFL
MRPIGGWLFGRIADRYGRKTSMLISVTMMCAGSLVIACLPTYAAIGAWAPFLLLVCRLFQGLSVGGEYGTTATYMSEVAL
RGQRGFFSSFQYVTLIGGQLLAVLVIVILETLLSEAELKAWGWRIPFVIGAISAVVALMLRRTLHETQSDENRKKDDAGT
IGGLFRHHKAAFFTVLGYTAGGSLIFYTFTTYMQKYLVNTVGLPIKTTSYVMTGALFLYMCMQPLFGALSDRIGRRNNML
LFGALGAIGTVPILTTLQHVTSPVAAFVLIVVALAIVSFYTSISGIVKAEMFPPEVRALGVGLAYAVANAIFGGSAEYVA
LGLKTLGHESAFFWYVTAMMVVAFLVSLRLPRQASYLHHDH

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=415, Percent_Identity=69.3975903614458, Blast_Score=553, Evalue=1e-159,
Organism=Escherichia coli, GI1790550, Length=379, Percent_Identity=32.1899736147757, Blast_Score=204, Evalue=9e-54,
Organism=Escherichia coli, GI1788292, Length=402, Percent_Identity=30.3482587064677, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1789941, Length=415, Percent_Identity=32.289156626506, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI87082404, Length=372, Percent_Identity=26.0752688172043, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87082231, Length=219, Percent_Identity=30.5936073059361, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48108; Mature: 47977

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCCCCHHHHCCCCC
>Mature Secondary Structure 
STHTPSAAGTPHPEPRAYTAEEKRHRVFSIMAASSGNLVEWFDFYVYAFAALYFAPAFF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCC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HHHHHHHHHCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]