Click here to switch to the map view.

The map label for this gene is proW [H]

Identifier: 120610182

GI number: 120610182

Start: 1659893

End: 1661248

Strand: Reverse

Name: proW [H]

Synonym: Aave_1496

Alternate gene names: 120610182

Gene position: 1661248-1659893 (Counterclockwise)

Preceding gene: 120610183

Following gene: 120610181

Centisome position: 31.04

GC content: 74.26

Gene sequence:

>1356_bases
ATGAACGCCACCCCGACGCCTCCTTCCACCTCCACGGGCACCGGCGCCCTCCAGGCCACCCAGGTGGCCCAGGCCCATCC
CGCTCCGGCGCCCGACGCCAACCCCTGGGCGGCAGCAGGCGACGGCCCCGCCGCCGTGGCTTCGTTCGGCACGCAGGCGA
CCCCTGCCACCGGCACGCCCCCCGCCGCACCCGCGGCAGACCCCTGGGCCGCCTCTTCGGCCACCGGCTCCGATGCCGGC
GGCGCGGACTGGCTGAACGCCGCGCCCGACCCAACCGCCGTCAGCGGCACGCTGGCCCACGGCGCCCCGGGCGGCGCGGG
CGATCCCTCGCAGGCCGCGGGCGGCCTGCACCTTTCCCAGCTCTGGGACGGATCGCTTCCGGTGGAACATTGGATCAACC
AGGGCCTGACCTGGACGGTGGAGCACTTCCGGCCCGCATTCCAGGCCGTGCGCGCACCGGTGGACGCCACGCTCGCCGGC
GTGCAGTCGGTGCTCACCGGCCTTCCCACGCTGGGCATGGTCGCCGTCGTGGGCCTTCTGGCATGGCAGTTCGCGGGCCG
CGCGCTCGCCCTGGGCGCGGTGGTCTCGCTGCTGCTGGTGGCGATGCTGGGCATCTGGCAGGAGGCGATGGTGACGCTCT
CGCTCGTGCTCACCTCGCTGGCGTTCTGCCTGGTGCTGGGACTGCCCCTGGGCATCCTGCTGGCCAGCAGCGACCGGGCA
CAGCGCCTGCTGCGCCCGGCGCTCGATGCCATGCAGACCACTCCGGCCTTCGTGTACCTCGTGCCGGTGGTGATGCTCTT
CGGCATCGGCAACGTGCCGGGCGTGGTCGTGACCATCGTGTTCGCGCTGCCGCCGCTGGTGCGCCTGACCAACCTGGGCC
TGCGGCAGGTGCGCCCCGACCTGGTGGAAGCCGCCCGCGCCTACGGTGCCTCGCCGCTGCAGATGCTCTGGAAAGTGCAG
CTGCCGCTGGCCATGCCGTCGATCATGGCCGGTATCAACCAGGCCCTGATGCTGTCGCTCTCGATGGTGGTGATCGCCTC
GATGATCGCCGTGGGCGGCCTGGGCCAGATGGTGCTGCGCGGCATCGGCCGGCTGGACATGGGCCTGGCCACCGTGGGCG
GACTGGGCATCGTGCTGCTCGCGATCACGCTGGACCGACTGACGCAAGCCATGGGCCAGCCGCGGCGCGGCGCGCGCCAC
TGGTGGCAGACCGGCCCCGCAGGCCTGGTGGTGGCCGCCTTCGCCGCACTCCGCCCGGCTCCCGGGCAAGCCCCCCGCGG
CCGCACCGCCGCCGCGGAACCCGCCCCCGCCCTGTCCGCCGCGGAAGAGCCCACGCTGGCGGGAGCCGCCCGCTGA

Upstream 100 bases:

>100_bases
TGCTGCGCTTCCTGGACCGCGATACCCCACCCGTGCCGCCGACGATGCCCCCCGCGTCCGCGCCCCACCCCGCCGCATCC
GAACCCGCTAGGAGCCACGC

Downstream 100 bases:

>100_bases
CGCCCCCCATCCAGGAACCACTTCTCCCTATGCACATCACTTTCCAACGCTCTTTTCCCGTTCCGGCGGGCGTGCCGGCC
TTCGCCCGCGCTGCCGCCTG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNATPTPPSTSTGTGALQATQVAQAHPAPAPDANPWAAAGDGPAAVASFGTQATPATGTPPAAPAADPWAASSATGSDAG
GADWLNAAPDPTAVSGTLAHGAPGGAGDPSQAAGGLHLSQLWDGSLPVEHWINQGLTWTVEHFRPAFQAVRAPVDATLAG
VQSVLTGLPTLGMVAVVGLLAWQFAGRALALGAVVSLLLVAMLGIWQEAMVTLSLVLTSLAFCLVLGLPLGILLASSDRA
QRLLRPALDAMQTTPAFVYLVPVVMLFGIGNVPGVVVTIVFALPPLVRLTNLGLRQVRPDLVEAARAYGASPLQMLWKVQ
LPLAMPSIMAGINQALMLSLSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGLGIVLLAITLDRLTQAMGQPRRGARH
WWQTGPAGLVVAAFAALRPAPGQAPRGRTAAAEPAPALSAAEEPTLAGAAR

Sequences:

>Translated_451_residues
MNATPTPPSTSTGTGALQATQVAQAHPAPAPDANPWAAAGDGPAAVASFGTQATPATGTPPAAPAADPWAASSATGSDAG
GADWLNAAPDPTAVSGTLAHGAPGGAGDPSQAAGGLHLSQLWDGSLPVEHWINQGLTWTVEHFRPAFQAVRAPVDATLAG
VQSVLTGLPTLGMVAVVGLLAWQFAGRALALGAVVSLLLVAMLGIWQEAMVTLSLVLTSLAFCLVLGLPLGILLASSDRA
QRLLRPALDAMQTTPAFVYLVPVVMLFGIGNVPGVVVTIVFALPPLVRLTNLGLRQVRPDLVEAARAYGASPLQMLWKVQ
LPLAMPSIMAGINQALMLSLSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGLGIVLLAITLDRLTQAMGQPRRGARH
WWQTGPAGLVVAAFAALRPAPGQAPRGRTAAAEPAPALSAAEEPTLAGAAR
>Mature_451_residues
MNATPTPPSTSTGTGALQATQVAQAHPAPAPDANPWAAAGDGPAAVASFGTQATPATGTPPAAPAADPWAASSATGSDAG
GADWLNAAPDPTAVSGTLAHGAPGGAGDPSQAAGGLHLSQLWDGSLPVEHWINQGLTWTVEHFRPAFQAVRAPVDATLAG
VQSVLTGLPTLGMVAVVGLLAWQFAGRALALGAVVSLLLVAMLGIWQEAMVTLSLVLTSLAFCLVLGLPLGILLASSDRA
QRLLRPALDAMQTTPAFVYLVPVVMLFGIGNVPGVVVTIVFALPPLVRLTNLGLRQVRPDLVEAARAYGASPLQMLWKVQ
LPLAMPSIMAGINQALMLSLSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGLGIVLLAITLDRLTQAMGQPRRGARH
WWQTGPAGLVVAAFAALRPAPGQAPRGRTAAAEPAPALSAAEEPTLAGAAR

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=348, Percent_Identity=58.3333333333333, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1788451, Length=180, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786564, Length=155, Percent_Identity=30.9677419354839, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788449, Length=154, Percent_Identity=29.2207792207792, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 45652; Mature: 45652

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATPTPPSTSTGTGALQATQVAQAHPAPAPDANPWAAAGDGPAAVASFGTQATPATGTP
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCC
PAAPAADPWAASSATGSDAGGADWLNAAPDPTAVSGTLAHGAPGGAGDPSQAAGGLHLSQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHH
LWDGSLPVEHWINQGLTWTVEHFRPAFQAVRAPVDATLAGVQSVLTGLPTLGMVAVVGLL
HCCCCCCHHHHHCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
AWQFAGRALALGAVVSLLLVAMLGIWQEAMVTLSLVLTSLAFCLVLGLPLGILLASSDRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
QRLLRPALDAMQTTPAFVYLVPVVMLFGIGNVPGVVVTIVFALPPLVRLTNLGLRQVRPD
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCHH
LVEAARAYGASPLQMLWKVQLPLAMPSIMAGINQALMLSLSMVVIASMIAVGGLGQMVLR
HHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
GIGRLDMGLATVGGLGIVLLAITLDRLTQAMGQPRRGARHWWQTGPAGLVVAAFAALRPA
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCC
PGQAPRGRTAAAEPAPALSAAEEPTLAGAAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNATPTPPSTSTGTGALQATQVAQAHPAPAPDANPWAAAGDGPAAVASFGTQATPATGTP
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCC
PAAPAADPWAASSATGSDAGGADWLNAAPDPTAVSGTLAHGAPGGAGDPSQAAGGLHLSQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHH
LWDGSLPVEHWINQGLTWTVEHFRPAFQAVRAPVDATLAGVQSVLTGLPTLGMVAVVGLL
HCCCCCCHHHHHCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
AWQFAGRALALGAVVSLLLVAMLGIWQEAMVTLSLVLTSLAFCLVLGLPLGILLASSDRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
QRLLRPALDAMQTTPAFVYLVPVVMLFGIGNVPGVVVTIVFALPPLVRLTNLGLRQVRPD
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCHH
LVEAARAYGASPLQMLWKVQLPLAMPSIMAGINQALMLSLSMVVIASMIAVGGLGQMVLR
HHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
GIGRLDMGLATVGGLGIVLLAITLDRLTQAMGQPRRGARHWWQTGPAGLVVAAFAALRPA
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCC
PGQAPRGRTAAAEPAPALSAAEEPTLAGAAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]