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The map label for this gene is thyA

Identifier: 120610137

GI number: 120610137

Start: 1597018

End: 1597857

Strand: Reverse

Name: thyA

Synonym: Aave_1450

Alternate gene names: 120610137

Gene position: 1597857-1597018 (Counterclockwise)

Preceding gene: 120610139

Following gene: 120610136

Centisome position: 29.85

GC content: 64.76

Gene sequence:

>840_bases
ATGAATGCCCCCGCCCGCCCCGTGCGCTCCCAGTACGAAGATTTCATGCGCCACGTCTATACCCATGGCGTGGCCAAGGG
CGACCGCACCGGCACCGGCACCCGCAGCGTGTTCGGCTACCAGATGCGGTTCGACCTGAACGAGGGCTTTCCACTGGTCA
CCACCAAGAAGGTCCACCTCAAATCCATCATCACGGAGCTGCTCTGGTTCCTCACCGGCTCCAGCAGCAACCACTGGCTG
AAGGAGCGCGGCGTCACCATCTGGGACGAATGGGCCCGCGAGGACGGCGACCTGGGGCCGGTCTACGGCGTGCAGTGGCG
CAGCTGGCCCACGCCGGACGGCGGCCATATCGACCAGATCAGCCAGGTGGTCGAAACGCTCAGGACCCATCCCGACTCGC
GCCGCATCATCGTCAGCGCCTGGAACGTGGCCGACCTGGACAAGATGGCCCTCATGCCCTGCCACGCGTTCTTCCAGTTC
TACGTGGCACCGCCGCAGGCCGCCGGAGAGCGGGGCAAACTGAGCTGCCAGTTGTACCAGCGCAGCGCCGACATCTTCCT
GGGAGTGCCCTTCAACATCGCGAGCTACGCGCTGCTGACCCACATGATGGCGCAGCAGTGCAATCTGGAGGTGGGCGATT
TCATCTGGACAGGCGGCGACTGCCACATCTACAGCAACCATTTCGAGCAGGTGGAGCTGCAGCTTTCTCGCGCCCCGCAC
CCCTATCCCACGCTGCACATCCTGCGCCGGCCGGACAGCCTCTTCGACTACCGTTTCGAGGATTTCGAAGTGCGCGACTA
CGCGCACCATCCCGCCATCAAGGCGCCGGTCGCGGTCTGA

Upstream 100 bases:

>100_bases
TAGCGCCTTCACGGGCTCCAGGCCCGGCTCCGGCAGCCCGCGGGCCCACACCCTAGAATCCACGGCTCCATCGTTGCGAA
CGCGCTTGCGCCTGCTGCCC

Downstream 100 bases:

>100_bases
CCATCCCGTTTTCCAAACGCTTCCAGCTTCCTCCCGATGTCTTCCCACGGCTTTCATGTCCATGGGCCGCACGACCACGC
TGTCGAGCATGCCGCCCAGC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MNAPARPVRSQYEDFMRHVYTHGVAKGDRTGTGTRSVFGYQMRFDLNEGFPLVTTKKVHLKSIITELLWFLTGSSSNHWL
KERGVTIWDEWAREDGDLGPVYGVQWRSWPTPDGGHIDQISQVVETLRTHPDSRRIIVSAWNVADLDKMALMPCHAFFQF
YVAPPQAAGERGKLSCQLYQRSADIFLGVPFNIASYALLTHMMAQQCNLEVGDFIWTGGDCHIYSNHFEQVELQLSRAPH
PYPTLHILRRPDSLFDYRFEDFEVRDYAHHPAIKAPVAV

Sequences:

>Translated_279_residues
MNAPARPVRSQYEDFMRHVYTHGVAKGDRTGTGTRSVFGYQMRFDLNEGFPLVTTKKVHLKSIITELLWFLTGSSSNHWL
KERGVTIWDEWAREDGDLGPVYGVQWRSWPTPDGGHIDQISQVVETLRTHPDSRRIIVSAWNVADLDKMALMPCHAFFQF
YVAPPQAAGERGKLSCQLYQRSADIFLGVPFNIASYALLTHMMAQQCNLEVGDFIWTGGDCHIYSNHFEQVELQLSRAPH
PYPTLHILRRPDSLFDYRFEDFEVRDYAHHPAIKAPVAV
>Mature_279_residues
MNAPARPVRSQYEDFMRHVYTHGVAKGDRTGTGTRSVFGYQMRFDLNEGFPLVTTKKVHLKSIITELLWFLTGSSSNHWL
KERGVTIWDEWAREDGDLGPVYGVQWRSWPTPDGGHIDQISQVVETLRTHPDSRRIIVSAWNVADLDKMALMPCHAFFQF
YVAPPQAAGERGKLSCQLYQRSADIFLGVPFNIASYALLTHMMAQQCNLEVGDFIWTGGDCHIYSNHFEQVELQLSRAPH
PYPTLHILRRPDSLFDYRFEDFEVRDYAHHPAIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=289, Percent_Identity=49.4809688581315, Blast_Score=277, Evalue=1e-74,
Organism=Escherichia coli, GI1789191, Length=270, Percent_Identity=68.8888888888889, Blast_Score=397, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71993377, Length=290, Percent_Identity=43.1034482758621, Blast_Score=247, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6324648, Length=230, Percent_Identity=48.695652173913, Blast_Score=208, Evalue=7e-55,
Organism=Drosophila melanogaster, GI17137556, Length=297, Percent_Identity=49.1582491582492, Blast_Score=280, Evalue=6e-76,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_ACIAC (A1TM53)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_969815.1
- ProteinModelPortal:   A1TM53
- SMR:   A1TM53
- STRING:   A1TM53
- GeneID:   4668056
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_1450
- NMPDR:   fig|397945.5.peg.1272
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- PhylomeDB:   A1TM53
- ProtClustDB:   PRK01827
- BioCyc:   AAVE397945:AAVE_1450-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 31979; Mature: 31979

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPARPVRSQYEDFMRHVYTHGVAKGDRTGTGTRSVFGYQMRFDLNEGFPLVTTKKVHL
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEECHHHH
KSIITELLWFLTGSSSNHWLKERGVTIWDEWAREDGDLGPVYGVQWRSWPTPDGGHIDQI
HHHHHHHHHHHCCCCCCCCHHHCCCEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
SQVVETLRTHPDSRRIIVSAWNVADLDKMALMPCHAFFQFYVAPPQAAGERGKLSCQLYQ
HHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEEE
RSADIFLGVPFNIASYALLTHMMAQQCNLEVGDFIWTGGDCHIYSNHFEQVELQLSRAPH
CCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCHHHHHHHHHCCCC
PYPTLHILRRPDSLFDYRFEDFEVRDYAHHPAIKAPVAV
CCCCEEEEECCCHHHCCCCCCCEEHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNAPARPVRSQYEDFMRHVYTHGVAKGDRTGTGTRSVFGYQMRFDLNEGFPLVTTKKVHL
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEECHHHH
KSIITELLWFLTGSSSNHWLKERGVTIWDEWAREDGDLGPVYGVQWRSWPTPDGGHIDQI
HHHHHHHHHHHCCCCCCCCHHHCCCEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
SQVVETLRTHPDSRRIIVSAWNVADLDKMALMPCHAFFQFYVAPPQAAGERGKLSCQLYQ
HHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEEE
RSADIFLGVPFNIASYALLTHMMAQQCNLEVGDFIWTGGDCHIYSNHFEQVELQLSRAPH
CCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCHHHHHHHHHCCCC
PYPTLHILRRPDSLFDYRFEDFEVRDYAHHPAIKAPVAV
CCCCEEEEECCCHHHCCCCCCCEEHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA