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The map label for this gene is tpiA

Identifier: 120609948

GI number: 120609948

Start: 1386478

End: 1387233

Strand: Direct

Name: tpiA

Synonym: Aave_1261

Alternate gene names: 120609948

Gene position: 1386478-1387233 (Clockwise)

Preceding gene: 120609947

Following gene: 120609949

Centisome position: 25.9

GC content: 70.37

Gene sequence:

>756_bases
ATGACAAAGAAGAAACTCATCGCGGGCAACTGGAAGATGAATGGCACCCTGGGTGCCAATGCCGCGCTGCTGCAGGCGCT
GCGCGGCGGCCTCGAGGCGGGCGCGTACCAGAACGTGGATGTGGCCGTGGCGGTGCCGGCGGCCTACCTCGCGCAGGTGC
AGGGCCTGGTCGAAGGCTCGGCCATCGCGCTGGCGGCGCAGGACGTGTCCCGGCACGAAGCGGGCGCCTACACGGGCGAG
GTCTCCGCGGCGATGCTCCAGGAATTCGGCGTGCGCTACGTGCTGGTCGGGCATTCCGAGCGCCGCCAGTACCACGGCGA
GACCGACGAGCAGGTGGCCGAGAAGGCGCAGCGCGCACTGGCCGCCGGCATCACGCCGGTGGTATGCGTGGGCGAGACGC
TGGCCGAGCGCGAGGCCGGACAGACCGAAGCCGTGGTCAAGCGCCAGCTGGCCGCGGTCATCCACCTGAACGGTCATTGC
ATCAGCGAGACCGTGGTGGCCTACGAGCCCGTCTGGGCCATCGGCACCGGTCGCACCGCCACGCCCGAGCAGGCACAGCA
GGTGCATGCCGTGCTGCGCGCGCAGCTCGCGGCTGCCAGCGAGCATGCGGACCGCATCCGCCTGCTCTACGGCGGCAGCA
TGAACGCCGCGAACGCGGCCGCATTGCTGGCCCAGCCCGACATCGACGGAGGCCTCGTGGGCGGCGCATCGCTCAAGGCC
CAGGACTTTTTGCAGATCATCGCAGCGGCCGCCTGA

Upstream 100 bases:

>100_bases
CCGTGGTGCACAGCACCTTCCCGGCGGCCGAGGCGTCCCGCGCCCATGCGCTCATGGAGTCGAGCCAGCATGCCGGCAAG
ATCGTTTTGACGTGGACAGC

Downstream 100 bases:

>100_bases
ACGGCACCGCGCAACCCAACCGGAGTAGAGAAGAAATGAATGTGCTCGTGAATGTGATCCTGGCCGTGCAGATGCTGGCC
GCGCTGGCGATGATCGGCCT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MTKKKLIAGNWKMNGTLGANAALLQALRGGLEAGAYQNVDVAVAVPAAYLAQVQGLVEGSAIALAAQDVSRHEAGAYTGE
VSAAMLQEFGVRYVLVGHSERRQYHGETDEQVAEKAQRALAAGITPVVCVGETLAEREAGQTEAVVKRQLAAVIHLNGHC
ISETVVAYEPVWAIGTGRTATPEQAQQVHAVLRAQLAAASEHADRIRLLYGGSMNAANAAALLAQPDIDGGLVGGASLKA
QDFLQIIAAAA

Sequences:

>Translated_251_residues
MTKKKLIAGNWKMNGTLGANAALLQALRGGLEAGAYQNVDVAVAVPAAYLAQVQGLVEGSAIALAAQDVSRHEAGAYTGE
VSAAMLQEFGVRYVLVGHSERRQYHGETDEQVAEKAQRALAAGITPVVCVGETLAEREAGQTEAVVKRQLAAVIHLNGHC
ISETVVAYEPVWAIGTGRTATPEQAQQVHAVLRAQLAAASEHADRIRLLYGGSMNAANAAALLAQPDIDGGLVGGASLKA
QDFLQIIAAAA
>Mature_250_residues
TKKKLIAGNWKMNGTLGANAALLQALRGGLEAGAYQNVDVAVAVPAAYLAQVQGLVEGSAIALAAQDVSRHEAGAYTGEV
SAAMLQEFGVRYVLVGHSERRQYHGETDEQVAEKAQRALAAGITPVVCVGETLAEREAGQTEAVVKRQLAAVIHLNGHCI
SETVVAYEPVWAIGTGRTATPEQAQQVHAVLRAQLAAASEHADRIRLLYGGSMNAANAAALLAQPDIDGGLVGGASLKAQ
DFLQIIAAAA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=250, Percent_Identity=44.8, Blast_Score=196, Evalue=1e-50,
Organism=Homo sapiens, GI4507645, Length=250, Percent_Identity=44.8, Blast_Score=196, Evalue=1e-50,
Organism=Escherichia coli, GI1790353, Length=250, Percent_Identity=50, Blast_Score=229, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17536593, Length=251, Percent_Identity=48.207171314741, Blast_Score=219, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6320255, Length=249, Percent_Identity=42.1686746987952, Blast_Score=196, Evalue=4e-51,
Organism=Drosophila melanogaster, GI28572004, Length=251, Percent_Identity=47.808764940239, Blast_Score=215, Evalue=2e-56,
Organism=Drosophila melanogaster, GI28572008, Length=251, Percent_Identity=47.808764940239, Blast_Score=214, Evalue=3e-56,
Organism=Drosophila melanogaster, GI28572006, Length=251, Percent_Identity=47.808764940239, Blast_Score=214, Evalue=3e-56,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_ACIAC (A1TLL4)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_969626.1
- ProteinModelPortal:   A1TLL4
- SMR:   A1TLL4
- STRING:   A1TLL4
- GeneID:   4668329
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_1261
- NMPDR:   fig|397945.5.peg.1106
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   A1TLL4
- ProtClustDB:   PRK00042
- BioCyc:   AAVE397945:AAVE_1261-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26039; Mature: 25908

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 98-98 ACT_SITE 169-169 BINDING 10-10 BINDING 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKKLIAGNWKMNGTLGANAALLQALRGGLEAGAYQNVDVAVAVPAAYLAQVQGLVEGS
CCCCCEEECCEEECCCCCCHHHHHHHHHCCHHCCCCCCCCEEEEHHHHHHHHHHHHHCCC
AIALAAQDVSRHEAGAYTGEVSAAMLQEFGVRYVLVGHSERRQYHGETDEQVAEKAQRAL
EEEEEHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHCCCCHHHHHHHHHHHH
AAGITPVVCVGETLAEREAGQTEAVVKRQLAAVIHLNGHCISETVVAYEPVWAIGTGRTA
HHCCCCEEECCHHHHHHCCCCHHHHHHHHHHHHHEECCCCHHHHHHHCCCEEEECCCCCC
TPEQAQQVHAVLRAQLAAASEHADRIRLLYGGSMNAANAAALLAQPDIDGGLVGGASLKA
CHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEECCCCCCCEECCCCCCH
QDFLQIIAAAA
HHHHHHHHHCC
>Mature Secondary Structure 
TKKKLIAGNWKMNGTLGANAALLQALRGGLEAGAYQNVDVAVAVPAAYLAQVQGLVEGS
CCCCEEECCEEECCCCCCHHHHHHHHHCCHHCCCCCCCCEEEEHHHHHHHHHHHHHCCC
AIALAAQDVSRHEAGAYTGEVSAAMLQEFGVRYVLVGHSERRQYHGETDEQVAEKAQRAL
EEEEEHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHCCCCHHHHHHHHHHHH
AAGITPVVCVGETLAEREAGQTEAVVKRQLAAVIHLNGHCISETVVAYEPVWAIGTGRTA
HHCCCCEEECCHHHHHHCCCCHHHHHHHHHHHHHEECCCCHHHHHHHCCCEEEECCCCCC
TPEQAQQVHAVLRAQLAAASEHADRIRLLYGGSMNAANAAALLAQPDIDGGLVGGASLKA
CHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEECCCCCCCEECCCCCCH
QDFLQIIAAAA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA