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The map label for this gene is pnp

Identifier: 120609946

GI number: 120609946

Start: 1383109

End: 1385385

Strand: Direct

Name: pnp

Synonym: Aave_1259

Alternate gene names: 120609946

Gene position: 1383109-1385385 (Clockwise)

Preceding gene: 120609945

Following gene: 120609947

Centisome position: 25.84

GC content: 66.89

Gene sequence:

>2277_bases
ATGAGCATGTTCAACAAGGTCACGAAAACGTTCCAGTGGGGCGACAAGACCGTCGTCATGGAAACGGGCGAGATCGCCCG
CCAGGCCACCGGCGCCGTGCTGGTCAACATCGACGACACGGTCGTGCTGGCCACGGTCGTCGCCTCCAAGTCCGCCAAGC
CCGGCCAGGATTTCTTCCCCCTGACGGTGGACTACATCGAGAAGACGTATGCCGCCGGCAAGATCCCCGGCAGCTTCTTC
AAGCGCGAAGCCAAGCCCAGCGAACTCGAGACGCTGACCAGCCGCCTGATCGACCGCCCGATCCGCCCGCTGTTCCCCGA
AGGCTTCTACAACGAAGTCCACGTGGTCATCCACACCGTGTCGCTGAACCCCGAGGTCGATGCCGACATCGCGGCCATGA
TCGCCGTGAGCGCGGCCCTGTCGGTGTCCGGCATTCCGTTCAACGGCCCGATCGGCGCCGCGCGCGTGGGCTACATCAAC
GGCGAATACGTGCTGAACCCGGGCCAGACCGCCCGCAAGAACTCGCAGATGGACCTGGTCGTCGCCGGCACCGAAGCCGC
CGTGCTGATGGTCGAGTCCGAGGCGCAGCAGCTGTCCGAAGACATCATGCTGGGCGCCGTGGTGTTCGGCCACGAGCAGG
GCCGCATCGCCATCAACGCCATCCATGAACTGGTGCGCGACGCCGGCAAGCCCGTGTGGGACTGGCAGCCCGCCGCCAAG
GACGAGCCCTTCATCGCCAAGGTCACGCAACTGGCCGAGGAGAAGCTGCGCGCCGCCTACCAGATCCGCAGCAAGCAGGC
CCGCACGCAGGCCCTGCGCGAGGCGTCCGCTTCCGTGCTGGAGTCGCTCAAGGCCGAAGGCGCCGATTTCGACGCGGTGA
AGGTCGAGGCCCTCCTGTTCGACATCGAAGCCAAGATCGTGCGCAGCCAGATCCTGGCCGGCGAGCCCCGCATCGACGGC
CGCGACACGCGCACCGTGCGCCCCATCGAGATCCGCAACTCCGTTCTGCCCCGCACGCACGGCTCCGCGCTGTTCACGCG
CGGCGAGACGCAGGCGCTGGTGGTCTCCACGCTGGGCACCGAACGCGACGCGCAGCGCATCGACGCGCTGGCCGGCGAGT
TCGAAGACCGCTTCATGTTCCACTACAACATGCCTCCCTTCGCCACCGGCGAAGTGGGCCGCATGGGCTCCACCAAGCGC
CGCGAGATCGGCCACGGCCGCCTGGCCAAGCGCGCGCTGGTCGCCGTGCTGCCGACGAAGGAAGAGTTCCCCTACACCAT
CCGCGTGGTGTCCGAGATCACCGAGTCCAACGGTTCGTCCTCGATGGCTTCCGTCTGCGGCGGCTGCCTGTCGATGATGG
ACGCCGGCGTGCCGATGAAGGCCCACGTGGCCGGCATCGCCATGGGCCTGATCAAGGAAGACAACCGCTTCGCGGTGCTG
ACCGACATCCTGGGCGATGAAGATCACCTGGGCGACATGGACTTCAAGGTGGCCGGCACCACGGCCGGCATCACCGCCCT
GCAGATGGACATCAAGATCCAGGGCATCACCAAGGAAATCATGCAGGTCGCCCTGGCCCAGGCCAAGGAAGCCCGCCTGC
ACATCCTCGGCAAGATGCAGGAAGCCATGGGCGAGGCCAAGACCGAGGTCTCCAGCTTCGCGCCCAAGCTCTACACGATG
AAGATCAACCCCGAGAAGATCCGCGACGTGATCGGCAAGGGCGGCGCGGTGATCCGCGCGCTGACGGAAGAGACCGGCAC
GCAGATCAACATCGACGAGGACGGCACGATCACCATCGCCTCCACCGACAGCGCGAAGGCCGACGAGGCCAAGCGCCGCA
TCGAGCAGATCACGGCCGAGGTCGAGATCGGCAAGATCTACGAAGGCCCCGTGGTCAAGATCCTGGACTTCGGCGCGCTG
ATCAACCTGCTGCCCGGCAAGGACGGCCTGCTGCACATCAGCCAGATCGCCCACGAGCGCGTGGAAAAGGTGACCGACTA
CCTGACCGAAGGCCAGGTCGTGAAGGTCAAGGTGCTCGAGACCGACGAGAAGGGCCGCGTGAAGCTGTCCATGCGTGCGC
TGCTGGACCGCCCGATGGGCGACAGCGGCCGTCCGGCGCCCGCCGAGCGTGGCGAACGGGGTGACCGCGGTGACCGTGGC
GACCGCCCGGAACGCGGCGAGCGCCGTGAGCGCCGCGAACCCGCCGGTGCAGACCAGCAGCAACAGCAGCAGCAATCCAC
TGGCGCGGGCGAGCACTCCGGCCAGATGGATGCCTGA

Upstream 100 bases:

>100_bases
GGTGCAGGCAGGGCCGCTGGAATGGCATCGTGTTCTGGATTGCTGCATCCACCCACCCGCCGGCATCTTTCCGTGCCGGC
CACACTCTGATGGAGCAACC

Downstream 100 bases:

>100_bases
GCTTCGGCCCCGCCTCCGGCGGCGGTGACCTCCGGACCGGCCCTGGCCGGTCCGGCCTCCTTTTGAAACCTGACATCCCT
GGGGATCCAGTATGGCGACC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MSMFNKVTKTFQWGDKTVVMETGEIARQATGAVLVNIDDTVVLATVVASKSAKPGQDFFPLTVDYIEKTYAAGKIPGSFF
KREAKPSELETLTSRLIDRPIRPLFPEGFYNEVHVVIHTVSLNPEVDADIAAMIAVSAALSVSGIPFNGPIGAARVGYIN
GEYVLNPGQTARKNSQMDLVVAGTEAAVLMVESEAQQLSEDIMLGAVVFGHEQGRIAINAIHELVRDAGKPVWDWQPAAK
DEPFIAKVTQLAEEKLRAAYQIRSKQARTQALREASASVLESLKAEGADFDAVKVEALLFDIEAKIVRSQILAGEPRIDG
RDTRTVRPIEIRNSVLPRTHGSALFTRGETQALVVSTLGTERDAQRIDALAGEFEDRFMFHYNMPPFATGEVGRMGSTKR
REIGHGRLAKRALVAVLPTKEEFPYTIRVVSEITESNGSSSMASVCGGCLSMMDAGVPMKAHVAGIAMGLIKEDNRFAVL
TDILGDEDHLGDMDFKVAGTTAGITALQMDIKIQGITKEIMQVALAQAKEARLHILGKMQEAMGEAKTEVSSFAPKLYTM
KINPEKIRDVIGKGGAVIRALTEETGTQINIDEDGTITIASTDSAKADEAKRRIEQITAEVEIGKIYEGPVVKILDFGAL
INLLPGKDGLLHISQIAHERVEKVTDYLTEGQVVKVKVLETDEKGRVKLSMRALLDRPMGDSGRPAPAERGERGDRGDRG
DRPERGERRERREPAGADQQQQQQQSTGAGEHSGQMDA

Sequences:

>Translated_758_residues
MSMFNKVTKTFQWGDKTVVMETGEIARQATGAVLVNIDDTVVLATVVASKSAKPGQDFFPLTVDYIEKTYAAGKIPGSFF
KREAKPSELETLTSRLIDRPIRPLFPEGFYNEVHVVIHTVSLNPEVDADIAAMIAVSAALSVSGIPFNGPIGAARVGYIN
GEYVLNPGQTARKNSQMDLVVAGTEAAVLMVESEAQQLSEDIMLGAVVFGHEQGRIAINAIHELVRDAGKPVWDWQPAAK
DEPFIAKVTQLAEEKLRAAYQIRSKQARTQALREASASVLESLKAEGADFDAVKVEALLFDIEAKIVRSQILAGEPRIDG
RDTRTVRPIEIRNSVLPRTHGSALFTRGETQALVVSTLGTERDAQRIDALAGEFEDRFMFHYNMPPFATGEVGRMGSTKR
REIGHGRLAKRALVAVLPTKEEFPYTIRVVSEITESNGSSSMASVCGGCLSMMDAGVPMKAHVAGIAMGLIKEDNRFAVL
TDILGDEDHLGDMDFKVAGTTAGITALQMDIKIQGITKEIMQVALAQAKEARLHILGKMQEAMGEAKTEVSSFAPKLYTM
KINPEKIRDVIGKGGAVIRALTEETGTQINIDEDGTITIASTDSAKADEAKRRIEQITAEVEIGKIYEGPVVKILDFGAL
INLLPGKDGLLHISQIAHERVEKVTDYLTEGQVVKVKVLETDEKGRVKLSMRALLDRPMGDSGRPAPAERGERGDRGDRG
DRPERGERRERREPAGADQQQQQQQSTGAGEHSGQMDA
>Mature_757_residues
SMFNKVTKTFQWGDKTVVMETGEIARQATGAVLVNIDDTVVLATVVASKSAKPGQDFFPLTVDYIEKTYAAGKIPGSFFK
REAKPSELETLTSRLIDRPIRPLFPEGFYNEVHVVIHTVSLNPEVDADIAAMIAVSAALSVSGIPFNGPIGAARVGYING
EYVLNPGQTARKNSQMDLVVAGTEAAVLMVESEAQQLSEDIMLGAVVFGHEQGRIAINAIHELVRDAGKPVWDWQPAAKD
EPFIAKVTQLAEEKLRAAYQIRSKQARTQALREASASVLESLKAEGADFDAVKVEALLFDIEAKIVRSQILAGEPRIDGR
DTRTVRPIEIRNSVLPRTHGSALFTRGETQALVVSTLGTERDAQRIDALAGEFEDRFMFHYNMPPFATGEVGRMGSTKRR
EIGHGRLAKRALVAVLPTKEEFPYTIRVVSEITESNGSSSMASVCGGCLSMMDAGVPMKAHVAGIAMGLIKEDNRFAVLT
DILGDEDHLGDMDFKVAGTTAGITALQMDIKIQGITKEIMQVALAQAKEARLHILGKMQEAMGEAKTEVSSFAPKLYTMK
INPEKIRDVIGKGGAVIRALTEETGTQINIDEDGTITIASTDSAKADEAKRRIEQITAEVEIGKIYEGPVVKILDFGALI
NLLPGKDGLLHISQIAHERVEKVTDYLTEGQVVKVKVLETDEKGRVKLSMRALLDRPMGDSGRPAPAERGERGDRGDRGD
RPERGERRERREPAGADQQQQQQQSTGAGEHSGQMDA

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=706, Percent_Identity=37.5354107648725, Blast_Score=428, Evalue=1e-120,
Organism=Escherichia coli, GI145693187, Length=690, Percent_Identity=65.2173913043478, Blast_Score=928, Evalue=0.0,
Organism=Escherichia coli, GI87082262, Length=118, Percent_Identity=35.5932203389831, Blast_Score=63, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI115534063, Length=664, Percent_Identity=32.5301204819277, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI281362905, Length=723, Percent_Identity=36.3762102351314, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651641, Length=723, Percent_Identity=36.3762102351314, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651643, Length=723, Percent_Identity=36.3762102351314, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI161079377, Length=670, Percent_Identity=36.1194029850746, Blast_Score=400, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_ACIAC (A1TLL2)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_969624.1
- ProteinModelPortal:   A1TLL2
- STRING:   A1TLL2
- GeneID:   4667468
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_1259
- NMPDR:   fig|397945.5.peg.1104
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   A1TLL2
- ProtClustDB:   PRK11824
- BioCyc:   AAVE397945:AAVE_1259-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 82447; Mature: 82316

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMFNKVTKTFQWGDKTVVMETGEIARQATGAVLVNIDDTVVLATVVASKSAKPGQDFFP
CCHHHHHHHHHCCCCEEEEEECHHHHHHCCCEEEEECCCCEEHHHHHHCCCCCCCCCCCC
LTVDYIEKTYAAGKIPGSFFKREAKPSELETLTSRLIDRPIRPLFPEGFYNEVHVVIHTV
CHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEE
SLNPEVDADIAAMIAVSAALSVSGIPFNGPIGAARVGYINGEYVLNPGQTARKNSQMDLV
ECCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEECCEEEECCCCCCCCCCCEEEE
VAGTEAAVLMVESEAQQLSEDIMLGAVVFGHEQGRIAINAIHELVRDAGKPVWDWQPAAK
EECCCEEEEEEEHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
DEPFIAKVTQLAEEKLRAAYQIRSKQARTQALREASASVLESLKAEGADFDAVKVEALLF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHH
DIEAKIVRSQILAGEPRIDGRDTRTVRPIEIRNSVLPRTHGSALFTRGETQALVVSTLGT
HHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHCCCCCCCCCCEEEECCCCCEEEEEECCC
ERDAQRIDALAGEFEDRFMFHYNMPPFATGEVGRMGSTKRREIGHGRLAKRALVAVLPTK
CHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHEEEECCC
EEFPYTIRVVSEITESNGSSSMASVCGGCLSMMDAGVPMKAHVAGIAMGLIKEDNRFAVL
CCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCEEEE
TDILGDEDHLGDMDFKVAGTTAGITALQMDIKIQGITKEIMQVALAQAKEARLHILGKMQ
EECCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
EAMGEAKTEVSSFAPKLYTMKINPEKIRDVIGKGGAVIRALTEETGTQINIDEDGTITIA
HHHHHHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCEEEEEHHHCCCEEEECCCCEEEEE
STDSAKADEAKRRIEQITAEVEIGKIYEGPVVKILDFGALINLLPGKDGLLHISQIAHER
ECCCCCHHHHHHHHHHHHHHEEECEECCCCEEEEEHHHHHHHHCCCCCCCCHHHHHHHHH
VEKVTDYLTEGQVVKVKVLETDEKGRVKLSMRALLDRPMGDSGRPAPAERGERGDRGDRG
HHHHHHHHCCCCEEEEEEEECCCCCCEEEEHHHHHCCCCCCCCCCCCHHCCCCCCCCCCC
DRPERGERRERREPAGADQQQQQQQSTGAGEHSGQMDA
CCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SMFNKVTKTFQWGDKTVVMETGEIARQATGAVLVNIDDTVVLATVVASKSAKPGQDFFP
CHHHHHHHHHCCCCEEEEEECHHHHHHCCCEEEEECCCCEEHHHHHHCCCCCCCCCCCC
LTVDYIEKTYAAGKIPGSFFKREAKPSELETLTSRLIDRPIRPLFPEGFYNEVHVVIHTV
CHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEE
SLNPEVDADIAAMIAVSAALSVSGIPFNGPIGAARVGYINGEYVLNPGQTARKNSQMDLV
ECCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEECCEEEECCCCCCCCCCCEEEE
VAGTEAAVLMVESEAQQLSEDIMLGAVVFGHEQGRIAINAIHELVRDAGKPVWDWQPAAK
EECCCEEEEEEEHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
DEPFIAKVTQLAEEKLRAAYQIRSKQARTQALREASASVLESLKAEGADFDAVKVEALLF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHH
DIEAKIVRSQILAGEPRIDGRDTRTVRPIEIRNSVLPRTHGSALFTRGETQALVVSTLGT
HHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHCCCCCCCCCCEEEECCCCCEEEEEECCC
ERDAQRIDALAGEFEDRFMFHYNMPPFATGEVGRMGSTKRREIGHGRLAKRALVAVLPTK
CHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHEEEECCC
EEFPYTIRVVSEITESNGSSSMASVCGGCLSMMDAGVPMKAHVAGIAMGLIKEDNRFAVL
CCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCEEEE
TDILGDEDHLGDMDFKVAGTTAGITALQMDIKIQGITKEIMQVALAQAKEARLHILGKMQ
EECCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
EAMGEAKTEVSSFAPKLYTMKINPEKIRDVIGKGGAVIRALTEETGTQINIDEDGTITIA
HHHHHHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCEEEEEHHHCCCEEEECCCCEEEEE
STDSAKADEAKRRIEQITAEVEIGKIYEGPVVKILDFGALINLLPGKDGLLHISQIAHER
ECCCCCHHHHHHHHHHHHHHEEECEECCCCEEEEEHHHHHHHHCCCCCCCCHHHHHHHHH
VEKVTDYLTEGQVVKVKVLETDEKGRVKLSMRALLDRPMGDSGRPAPAERGERGDRGDRG
HHHHHHHHCCCCEEEEEEEECCCCCCEEEEHHHHHCCCCCCCCCCCCHHCCCCCCCCCCC
DRPERGERRERREPAGADQQQQQQQSTGAGEHSGQMDA
CCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA