Click here to switch to the map view.

The map label for this gene is dnaB [H]

Identifier: 120609919

GI number: 120609919

Start: 1356082

End: 1357503

Strand: Direct

Name: dnaB [H]

Synonym: Aave_1232

Alternate gene names: 120609919

Gene position: 1356082-1357503 (Clockwise)

Preceding gene: 120609918

Following gene: 120609924

Centisome position: 25.33

GC content: 65.47

Gene sequence:

>1422_bases
ATGAGTTCCGACTTCCCTCCGCTGCACGAATCCGATTTCGCGTCTGCCGCCCCGGCGGACCAGGAGGTGGCCCGGCTGCG
CATGCCACCGCATTCGCTGGAGGCCGAGTCCAGCGTGCTGGGCGGCCTGCTGCTCGACAACATGGCCTGGGACCGGGTGG
GTGACCTGCTGGTGGACGGGGATTTCTACCGGCACGAGCACCAGCTCATCTATTCGGCCATCGGCAAGCTGGTGAATGCC
AGCAAGCCGGCGGACGTGATCACGGTCTTCGGCGAGCTGCAGAGCCTGGGCAAGGCCGAGGAGGTGGGCGGGCTGGCCTA
TCTCAACAATCTGGCGCAGTACGTTCCCAGCGCCACCAACATCCGCCGCTACGCGGAGATCGTGCGCGAGCGCTCCATCC
TGCGCAAGCTCGTCACGGCGAGCGACGAAATCTCCACGACGGCCTTCAATCCGAAGGGAAAGACGGTCGAGAAGATCCTG
GACGAGGCCGAATCCAAGATCTTCGCCATCGGCGAAGAGGGCTCGCGCAACAAGCAGGGCTTCCAGTCGCTCGACACCCT
CGTGGTGGACCTGCTGGACCAGGTGCAGGAGATGGCGGACAACCCGATGGACGTGACCGGGGTCCCCACCGGCTTCGCCG
ACCTGGACCGCATGACCTCGGGACTGCAGGCGGGCGACATGATCGTGCTGGCCGCGCGCCCTTCGATGGGCAAGACCTCG
TTCGCCGTGAACATTGCCGAGCACGTGGCGCTCAACGAAGGCCTTCCCGTGGCCATCTTTTCGATGGAAATGGGCGCCGC
CCAGCTGGCCGTGCGTATCGTGGGCTCGATCGGCCGCGTGAACCAGGGCAACCTGCGGACCGGCAAGCTCACGGACGAGG
AATGGCCGCGCCTCACCGAGGCGATCGAGCGGCTGCGTACCGTCTCGCTGCACATCGACGAAACGCCGGGGCTCACCCCG
GGGGAACTGCGGGCCAATGCCCGCCGTCTGGCACGCCAGTGCGGCAAGCTCGGCTTGATCGTGGTGGACTACCTGCAGCT
CATGAGCGGCTCGGGCGCGGGCAGTGCCGACAACCGGGCCACCGAACTGGGCGAAATCTCGCGGGGGCTGAAGATGCTGG
CCAAGGAACTGCAGTGCCCGGTGATCGCGCTATCGCAGCTCAACCGCTCGGTGGAGCAGCGCACCGACAAGCGTCCCATG
ATGTCCGACCTGCGCGAATCCGGCGCCATCGAGCAGGACGCGGACATCATCATGTTCATCTACCGCGACGACTACTACAA
CAAGGACTCCAAGGAGCCCAACGTGGCCGAGGTCATCATCGGCAAGCAGCGGAACGGCCCCACCGGCACGGTGAAGCTGT
TCTTCCAGAAGAACCAGACGCGGTTCGAGAACCTCGCCATGGGGTCGGGCGACGATTTCTGA

Upstream 100 bases:

>100_bases
CCGCCAGCCCACCGGCTGTGAACGGCTTTTCCACAGACTTGTCCACTGACCGACAGTTTCCGGCCCCGCTGTGGTGCCGG
AAGCGCAAGGATCGATTGCC

Downstream 100 bases:

>100_bases
CGCCGGCCAGGACGGGCCTGCGCCGCCCTACGCCCTGGCGACGGTGTAGGCCACCATCCCCAGCAGCACCACGCCCAGCA
CCCCGCAGGCCACCGCCAGG

Product: primary replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSSDFPPLHESDFASAAPADQEVARLRMPPHSLEAESSVLGGLLLDNMAWDRVGDLLVDGDFYRHEHQLIYSAIGKLVNA
SKPADVITVFGELQSLGKAEEVGGLAYLNNLAQYVPSATNIRRYAEIVRERSILRKLVTASDEISTTAFNPKGKTVEKIL
DEAESKIFAIGEEGSRNKQGFQSLDTLVVDLLDQVQEMADNPMDVTGVPTGFADLDRMTSGLQAGDMIVLAARPSMGKTS
FAVNIAEHVALNEGLPVAIFSMEMGAAQLAVRIVGSIGRVNQGNLRTGKLTDEEWPRLTEAIERLRTVSLHIDETPGLTP
GELRANARRLARQCGKLGLIVVDYLQLMSGSGAGSADNRATELGEISRGLKMLAKELQCPVIALSQLNRSVEQRTDKRPM
MSDLRESGAIEQDADIIMFIYRDDYYNKDSKEPNVAEVIIGKQRNGPTGTVKLFFQKNQTRFENLAMGSGDDF

Sequences:

>Translated_473_residues
MSSDFPPLHESDFASAAPADQEVARLRMPPHSLEAESSVLGGLLLDNMAWDRVGDLLVDGDFYRHEHQLIYSAIGKLVNA
SKPADVITVFGELQSLGKAEEVGGLAYLNNLAQYVPSATNIRRYAEIVRERSILRKLVTASDEISTTAFNPKGKTVEKIL
DEAESKIFAIGEEGSRNKQGFQSLDTLVVDLLDQVQEMADNPMDVTGVPTGFADLDRMTSGLQAGDMIVLAARPSMGKTS
FAVNIAEHVALNEGLPVAIFSMEMGAAQLAVRIVGSIGRVNQGNLRTGKLTDEEWPRLTEAIERLRTVSLHIDETPGLTP
GELRANARRLARQCGKLGLIVVDYLQLMSGSGAGSADNRATELGEISRGLKMLAKELQCPVIALSQLNRSVEQRTDKRPM
MSDLRESGAIEQDADIIMFIYRDDYYNKDSKEPNVAEVIIGKQRNGPTGTVKLFFQKNQTRFENLAMGSGDDF
>Mature_472_residues
SSDFPPLHESDFASAAPADQEVARLRMPPHSLEAESSVLGGLLLDNMAWDRVGDLLVDGDFYRHEHQLIYSAIGKLVNAS
KPADVITVFGELQSLGKAEEVGGLAYLNNLAQYVPSATNIRRYAEIVRERSILRKLVTASDEISTTAFNPKGKTVEKILD
EAESKIFAIGEEGSRNKQGFQSLDTLVVDLLDQVQEMADNPMDVTGVPTGFADLDRMTSGLQAGDMIVLAARPSMGKTSF
AVNIAEHVALNEGLPVAIFSMEMGAAQLAVRIVGSIGRVNQGNLRTGKLTDEEWPRLTEAIERLRTVSLHIDETPGLTPG
ELRANARRLARQCGKLGLIVVDYLQLMSGSGAGSADNRATELGEISRGLKMLAKELQCPVIALSQLNRSVEQRTDKRPMM
SDLRESGAIEQDADIIMFIYRDDYYNKDSKEPNVAEVIIGKQRNGPTGTVKLFFQKNQTRFENLAMGSGDDF

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=455, Percent_Identity=49.010989010989, Blast_Score=453, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51715; Mature: 51584

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDFPPLHESDFASAAPADQEVARLRMPPHSLEAESSVLGGLLLDNMAWDRVGDLLVDG
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECC
DFYRHEHQLIYSAIGKLVNASKPADVITVFGELQSLGKAEEVGGLAYLNNLAQYVPSATN
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHH
IRRYAEIVRERSILRKLVTASDEISTTAFNPKGKTVEKILDEAESKIFAIGEEGSRNKQG
HHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHCCCEEEECCCCCCCHHH
FQSLDTLVVDLLDQVQEMADNPMDVTGVPTGFADLDRMTSGLQAGDMIVLAARPSMGKTS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCH
FAVNIAEHVALNEGLPVAIFSMEMGAAQLAVRIVGSIGRVNQGNLRTGKLTDEEWPRLTE
HEEHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
AIERLRTVSLHIDETPGLTPGELRANARRLARQCGKLGLIVVDYLQLMSGSGAGSADNRA
HHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHH
TELGEISRGLKMLAKELQCPVIALSQLNRSVEQRTDKRPMMSDLRESGAIEQDADIIMFI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEE
YRDDYYNKDSKEPNVAEVIIGKQRNGPTGTVKLFFQKNQTRFENLAMGSGDDF
EECCCCCCCCCCCCHHHEEEECCCCCCCCEEEEEEECCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
SSDFPPLHESDFASAAPADQEVARLRMPPHSLEAESSVLGGLLLDNMAWDRVGDLLVDG
CCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECC
DFYRHEHQLIYSAIGKLVNASKPADVITVFGELQSLGKAEEVGGLAYLNNLAQYVPSATN
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHH
IRRYAEIVRERSILRKLVTASDEISTTAFNPKGKTVEKILDEAESKIFAIGEEGSRNKQG
HHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHCCCEEEECCCCCCCHHH
FQSLDTLVVDLLDQVQEMADNPMDVTGVPTGFADLDRMTSGLQAGDMIVLAARPSMGKTS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCH
FAVNIAEHVALNEGLPVAIFSMEMGAAQLAVRIVGSIGRVNQGNLRTGKLTDEEWPRLTE
HEEHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
AIERLRTVSLHIDETPGLTPGELRANARRLARQCGKLGLIVVDYLQLMSGSGAGSADNRA
HHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHH
TELGEISRGLKMLAKELQCPVIALSQLNRSVEQRTDKRPMMSDLRESGAIEQDADIIMFI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEE
YRDDYYNKDSKEPNVAEVIIGKQRNGPTGTVKLFFQKNQTRFENLAMGSGDDF
EECCCCCCCCCCCCHHHEEEECCCCCCCCEEEEEEECCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]