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The map label for this gene is truA

Identifier: 120609904

GI number: 120609904

Start: 1339436

End: 1340272

Strand: Reverse

Name: truA

Synonym: Aave_1217

Alternate gene names: 120609904

Gene position: 1340272-1339436 (Counterclockwise)

Preceding gene: 120609905

Following gene: 120609903

Centisome position: 25.04

GC content: 70.85

Gene sequence:

>837_bases
ATGAGGGTGGCGCTGGGCGTCAGCTACAACGGTTCCGCCTACCGCGGCTGGCAGAGCCAGCCCGGGGGGGCGACCGTGCA
GGACCGCCTGGAGGCGGCCCTCGCGCGCTTCGCCACGCACGAGGTCTCCACGATCTGTGCCGGGCGCACGGATGCGGGGG
TGCACGGCCTCATGCAGGTGGTGCACTTCGACACGCCGCTGGAGCGGACGCCGTTCTCCTGGGTGCGCGGCACCAATACC
TTCCTGCCGCCGGACATCGCCGTGCAATGGGCGCGGCCCGTGCCGGAAGCCTTCCATTCGCGCGCCAGCGCCACGGCCCG
CCGGTACGCCTACGTGCTGCTGCAGTCGCCCGTGCGCCCGAGCGTGGAGGCGGGACGGGTGGGCTGGGTCTTCCACGCGC
TGGACGGGGATGCCATGCGCGCTGCGGCACGGCACCTGCTCGGAGAGCACGACTTCACCTCCTTCCGCGCATCCGCATGC
CAGGCCAAGTCACCCGTCAAGACGCTGCGCCGCATAGAGATCACGCAGCGCGGCACGGGCGGGGTGCCGGCAGGGCAGGC
CCCGGGCGACCGTGGCGACGTACCCACCTGCTACTGGCGATTCGAGTTCGAGGGCAATGCCTTCCTGCACCACATGGTGC
GCAACATCATGGGATGCCTCGTGGCGGTCGGCCAGGGACAGCATCCGGCGGACTGGATGCACGCCGTGCTGCAGGCGCGC
TCGCGGGACGCGGCGGCGCCCACCTTCTCCCCGGACGGTTTGTACTTTCTCGGTCCGCTCTACGATCCGGCCTGGGGACT
GCCCGATCGCACCGCTGCGTATGATTGGCTGCCCTGA

Upstream 100 bases:

>100_bases
TCGGGCCCGCTCAAGGCCCGGGCACAGCGCCTGCTGGCCGAACTGGGCTGATGGTCCGGCAGTCCGCGACCGGGCGGGCC
CGGCGCACGGGCGGGCGGCC

Downstream 100 bases:

>100_bases
CGGCAGGCCCCGCGGCTGAGCCGTGCATTGCCCGGCGCCCGGCGGGCCCGCTACGCGCCTGCGGCAGCCCATCCGCATAC
CTTCATGTCTTCGCCCATGC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MRVALGVSYNGSAYRGWQSQPGGATVQDRLEAALARFATHEVSTICAGRTDAGVHGLMQVVHFDTPLERTPFSWVRGTNT
FLPPDIAVQWARPVPEAFHSRASATARRYAYVLLQSPVRPSVEAGRVGWVFHALDGDAMRAAARHLLGEHDFTSFRASAC
QAKSPVKTLRRIEITQRGTGGVPAGQAPGDRGDVPTCYWRFEFEGNAFLHHMVRNIMGCLVAVGQGQHPADWMHAVLQAR
SRDAAAPTFSPDGLYFLGPLYDPAWGLPDRTAAYDWLP

Sequences:

>Translated_278_residues
MRVALGVSYNGSAYRGWQSQPGGATVQDRLEAALARFATHEVSTICAGRTDAGVHGLMQVVHFDTPLERTPFSWVRGTNT
FLPPDIAVQWARPVPEAFHSRASATARRYAYVLLQSPVRPSVEAGRVGWVFHALDGDAMRAAARHLLGEHDFTSFRASAC
QAKSPVKTLRRIEITQRGTGGVPAGQAPGDRGDVPTCYWRFEFEGNAFLHHMVRNIMGCLVAVGQGQHPADWMHAVLQAR
SRDAAAPTFSPDGLYFLGPLYDPAWGLPDRTAAYDWLP
>Mature_278_residues
MRVALGVSYNGSAYRGWQSQPGGATVQDRLEAALARFATHEVSTICAGRTDAGVHGLMQVVHFDTPLERTPFSWVRGTNT
FLPPDIAVQWARPVPEAFHSRASATARRYAYVLLQSPVRPSVEAGRVGWVFHALDGDAMRAAARHLLGEHDFTSFRASAC
QAKSPVKTLRRIEITQRGTGGVPAGQAPGDRGDVPTCYWRFEFEGNAFLHHMVRNIMGCLVAVGQGQHPADWMHAVLQAR
SRDAAAPTFSPDGLYFLGPLYDPAWGLPDRTAAYDWLP

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=250, Percent_Identity=36, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI199559785, Length=288, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1788657, Length=267, Percent_Identity=42.6966292134831, Blast_Score=222, Evalue=2e-59,
Organism=Drosophila melanogaster, GI116007752, Length=281, Percent_Identity=28.8256227758007, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_ACIAC (A1TLH0)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_969582.1
- ProteinModelPortal:   A1TLH0
- SMR:   A1TLH0
- STRING:   A1TLH0
- GeneID:   4665455
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_1217
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   A1TLH0
- ProtClustDB:   PRK00021
- BioCyc:   AAVE397945:AAVE_1217-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 30452; Mature: 30452

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVALGVSYNGSAYRGWQSQPGGATVQDRLEAALARFATHEVSTICAGRTDAGVHGLMQV
CEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VHFDTPLERTPFSWVRGTNTFLPPDIAVQWARPVPEAFHSRASATARRYAYVLLQSPVRP
HHCCCCCCCCCCHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHEEEEECCCCCC
SVEAGRVGWVFHALDGDAMRAAARHLLGEHDFTSFRASACQAKSPVKTLRRIEITQRGTG
CCCCCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
GVPAGQAPGDRGDVPTCYWRFEFEGNAFLHHMVRNIMGCLVAVGQGQHPADWMHAVLQAR
CCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
SRDAAAPTFSPDGLYFLGPLYDPAWGLPDRTAAYDWLP
CCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRVALGVSYNGSAYRGWQSQPGGATVQDRLEAALARFATHEVSTICAGRTDAGVHGLMQV
CEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VHFDTPLERTPFSWVRGTNTFLPPDIAVQWARPVPEAFHSRASATARRYAYVLLQSPVRP
HHCCCCCCCCCCHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHEEEEECCCCCC
SVEAGRVGWVFHALDGDAMRAAARHLLGEHDFTSFRASACQAKSPVKTLRRIEITQRGTG
CCCCCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
GVPAGQAPGDRGDVPTCYWRFEFEGNAFLHHMVRNIMGCLVAVGQGQHPADWMHAVLQAR
CCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
SRDAAAPTFSPDGLYFLGPLYDPAWGLPDRTAAYDWLP
CCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA