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The map label for this gene is wcaJ [H]

Identifier: 120609896

GI number: 120609896

Start: 1331577

End: 1332887

Strand: Reverse

Name: wcaJ [H]

Synonym: Aave_1209

Alternate gene names: 120609896

Gene position: 1332887-1331577 (Counterclockwise)

Preceding gene: 120609897

Following gene: 120609894

Centisome position: 24.9

GC content: 68.34

Gene sequence:

>1311_bases
ATGAACAGCATTCCCCAACGCGAACTTGGCTCTTCCACGGCGCACCGGCAAGGCGCGCTGCTGCGCTGGTCGCTGGCGGG
AACGGTGACGCTGCTCGCCGCCTATCTGATCGGGCTGGAACTGCAGCGCCGCGGCTGGGCGACCTACCAGTTCCCCGCAA
CCGTCCTGTGGTGCGCCATCCCCTACGCCATCGCGTCGTACTGGCTGTACAGCGGCGCCCACCTGCCGCGTTTCGAGGGC
AGGAGCTTTCTCTTCGTGGCGACGGCCGTGCCGTACCTGGCGGCTCCGCTCGGCTTCGCGCTGCTGCAGCGCCCCTATTC
CCGGGGAGCCGTGTTGCTGTCGTACCTGCTGTCGCTGGCGTGGTTCCTGGCCGGCCATTGGCGCGACCTGCGCCGGCACG
GCCTGTGCCTGATCTATCTGGACGATACCGTCCCGCAGCGCCTGCAGGCGCTCCTGGGTCCCGGCGCAGACGAAGCGCGC
AGAAAGGACATCACGCTCATGCCCTGGCCGGAGCGGGGCCTGGACGACGCAGCCGCCTCGGGCTGGGCCGGCGTGGTGCT
GGACCGGCATGCCCCCTCCCACGCGGCCCGCGACCGGAAAATCAGCGGTTTGCGGCTGCAGCACATCCGCCTCTACAGTG
TCGAAGCGGTGGCCGAACTGCTGAGCGGCCGGATGGTGCTGCCTGAAAACCATGCGCAGGATTTGTGGACACTGGACGGC
AATCCGGCCTACGACACGGTCAAGCGCATGGTGGACCTGGCCGCCACGCTGGCCACACTGCCGCTTTGGCTTCCCCTGTG
CCTGGTCGCGGGCCTGGCGGTGAAGCTCGATTCCCCCGGCCCCATGCTCTTTTCGCAGTTGCGCGTGGGCCGCAACGGGC
GCGAATTCTGCATCTGGAAGCTGCGCAGCATGCGCCATGCGCCAGCGGAGCAAGGCGCGCGTTTCGCGCAGCCCAACGAC
GATCGCATCACGCCGGTGGGCCGCTTCATACGCCGCACCCGCCTCGACGAGCTGCCCCAGTTGTGGAACGTCCTGCGGGG
CGACATGAGCCTGATCGGCCCGCGGCCCGAACAGGCGGAATTCGTGTACAGCTTCGCGGAACGCATCCCCGCCTACCCCT
ACCGCCACCTGGTGCGTCCCGGCCTGACCGGCTGGGCGCAGGTGCAGCAGGGCTACGCGGACAGCGAGGAGCAGACCGCC
GTCAAGCTCAGCTACGACCTCTACTACGTCGCGCACTATTCCGTGGCACTGGATCTGCTGATCGCCTGGAAGACGCTGCG
CATCGTGGCCAGCGGATTCGGCTCCCGGTAG

Upstream 100 bases:

>100_bases
CACGGCAGCGCTACGAACGGCACTACACCGCCGAGCGCATGGGGTCTGCGGTGCGCGACGTCTATGCCGCCGCCCTGCGG
CGTTCCCGCTCCTGAATGGC

Downstream 100 bases:

>100_bases
CAGCGTGGCCTCAGGACATTCCCACCCGCGCGCGCGCGACCAGCACGTCCGCCCCCAGCGGCCGCCGGCGCACGAGGCCC
CGCAGCAGGCGCCCCTTGAG

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MNSIPQRELGSSTAHRQGALLRWSLAGTVTLLAAYLIGLELQRRGWATYQFPATVLWCAIPYAIASYWLYSGAHLPRFEG
RSFLFVATAVPYLAAPLGFALLQRPYSRGAVLLSYLLSLAWFLAGHWRDLRRHGLCLIYLDDTVPQRLQALLGPGADEAR
RKDITLMPWPERGLDDAAASGWAGVVLDRHAPSHAARDRKISGLRLQHIRLYSVEAVAELLSGRMVLPENHAQDLWTLDG
NPAYDTVKRMVDLAATLATLPLWLPLCLVAGLAVKLDSPGPMLFSQLRVGRNGREFCIWKLRSMRHAPAEQGARFAQPND
DRITPVGRFIRRTRLDELPQLWNVLRGDMSLIGPRPEQAEFVYSFAERIPAYPYRHLVRPGLTGWAQVQQGYADSEEQTA
VKLSYDLYYVAHYSVALDLLIAWKTLRIVASGFGSR

Sequences:

>Translated_436_residues
MNSIPQRELGSSTAHRQGALLRWSLAGTVTLLAAYLIGLELQRRGWATYQFPATVLWCAIPYAIASYWLYSGAHLPRFEG
RSFLFVATAVPYLAAPLGFALLQRPYSRGAVLLSYLLSLAWFLAGHWRDLRRHGLCLIYLDDTVPQRLQALLGPGADEAR
RKDITLMPWPERGLDDAAASGWAGVVLDRHAPSHAARDRKISGLRLQHIRLYSVEAVAELLSGRMVLPENHAQDLWTLDG
NPAYDTVKRMVDLAATLATLPLWLPLCLVAGLAVKLDSPGPMLFSQLRVGRNGREFCIWKLRSMRHAPAEQGARFAQPND
DRITPVGRFIRRTRLDELPQLWNVLRGDMSLIGPRPEQAEFVYSFAERIPAYPYRHLVRPGLTGWAQVQQGYADSEEQTA
VKLSYDLYYVAHYSVALDLLIAWKTLRIVASGFGSR
>Mature_436_residues
MNSIPQRELGSSTAHRQGALLRWSLAGTVTLLAAYLIGLELQRRGWATYQFPATVLWCAIPYAIASYWLYSGAHLPRFEG
RSFLFVATAVPYLAAPLGFALLQRPYSRGAVLLSYLLSLAWFLAGHWRDLRRHGLCLIYLDDTVPQRLQALLGPGADEAR
RKDITLMPWPERGLDDAAASGWAGVVLDRHAPSHAARDRKISGLRLQHIRLYSVEAVAELLSGRMVLPENHAQDLWTLDG
NPAYDTVKRMVDLAATLATLPLWLPLCLVAGLAVKLDSPGPMLFSQLRVGRNGREFCIWKLRSMRHAPAEQGARFAQPND
DRITPVGRFIRRTRLDELPQLWNVLRGDMSLIGPRPEQAEFVYSFAERIPAYPYRHLVRPGLTGWAQVQQGYADSEEQTA
VKLSYDLYYVAHYSVALDLLIAWKTLRIVASGFGSR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=206, Percent_Identity=42.7184466019417, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 48937; Mature: 48937

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIPQRELGSSTAHRQGALLRWSLAGTVTLLAAYLIGLELQRRGWATYQFPATVLWCAI
CCCCCCHHHCCCHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHH
PYAIASYWLYSGAHLPRFEGRSFLFVATAVPYLAAPLGFALLQRPYSRGAVLLSYLLSLA
HHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
WFLAGHWRDLRRHGLCLIYLDDTVPQRLQALLGPGADEARRKDITLMPWPERGLDDAAAS
HHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCCCHHHHCCCEECCCCCCCCCHHHCC
GWAGVVLDRHAPSHAARDRKISGLRLQHIRLYSVEAVAELLSGRMVLPENHAQDLWTLDG
CCEEEEEECCCCCHHHCCCCCCCEEEEHEEEHHHHHHHHHHCCCEECCCCCCCCEEECCC
NPAYDTVKRMVDLAATLATLPLWLPLCLVAGLAVKLDSPGPMLFSQLRVGRNGREFCIWK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCHHHHHH
LRSMRHAPAEQGARFAQPNDDRITPVGRFIRRTRLDELPQLWNVLRGDMSLIGPRPEQAE
HHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHH
FVYSFAERIPAYPYRHLVRPGLTGWAQVQQGYADSEEQTAVKLSYDLYYVAHYSVALDLL
HHHHHHHHCCCCCHHHHHCCCCCHHHHHHHCCCCCCCCEEEEEEEHEEEEHHHHHHHHHH
IAWKTLRIVASGFGSR
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNSIPQRELGSSTAHRQGALLRWSLAGTVTLLAAYLIGLELQRRGWATYQFPATVLWCAI
CCCCCCHHHCCCHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHH
PYAIASYWLYSGAHLPRFEGRSFLFVATAVPYLAAPLGFALLQRPYSRGAVLLSYLLSLA
HHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
WFLAGHWRDLRRHGLCLIYLDDTVPQRLQALLGPGADEARRKDITLMPWPERGLDDAAAS
HHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCCCHHHHCCCEECCCCCCCCCHHHCC
GWAGVVLDRHAPSHAARDRKISGLRLQHIRLYSVEAVAELLSGRMVLPENHAQDLWTLDG
CCEEEEEECCCCCHHHCCCCCCCEEEEHEEEHHHHHHHHHHCCCEECCCCCCCCEEECCC
NPAYDTVKRMVDLAATLATLPLWLPLCLVAGLAVKLDSPGPMLFSQLRVGRNGREFCIWK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCHHHHHH
LRSMRHAPAEQGARFAQPNDDRITPVGRFIRRTRLDELPQLWNVLRGDMSLIGPRPEQAE
HHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHH
FVYSFAERIPAYPYRHLVRPGLTGWAQVQQGYADSEEQTAVKLSYDLYYVAHYSVALDLL
HHHHHHHHCCCCCHHHHHCCCCCHHHHHHHCCCCCCCCEEEEEEEHEEEEHHHHHHHHHH
IAWKTLRIVASGFGSR
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]