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The map label for this gene is epsE [H]

Identifier: 120609761

GI number: 120609761

Start: 1170904

End: 1172745

Strand: Direct

Name: epsE [H]

Synonym: Aave_1068

Alternate gene names: 120609761

Gene position: 1170904-1172745 (Clockwise)

Preceding gene: 120609760

Following gene: 120609762

Centisome position: 21.87

GC content: 71.06

Gene sequence:

>1842_bases
ATGAGCCGCAGCCTGCCTCCTACCCTCCATGACGCCGCCGGGCCCGCGGCGCCGGCCGGCTTCGCGGGCCCCCTCGAGTG
GCGGCGCATCGTCGAGTGGCTGCGCGCCGACGGCGTGATCTCGCAGGAGGAGGCCGCGCGCACCATCGCGCGCTGCTCGC
AGGCCGAGAGCCGGCAGCATCCGCTGGTGCGGCTGGCCAGCGTGGCGATGGCGCGCGCGAGCGACGGCAGGCCGCTGGAC
CTGGAGAGCCTGACCGAATACCTGGCCGGCCGCAGCGGCCTGGCCTACCTGCGCATCGATCCGCTCAAGGTGGACGTGGG
CCGCGTGGCCGACATGATGAGCGCGAGCTACGCGGAGCGGCACCGGGTGCTGCCCGTGCAGGTCACGCCGCGCGAGATCG
TGGTGGCCACGGCCGAGCCGTTCGTGGACGACTGGGTGGCCGAGGTGGAACGCCAGTCGCGCCGCGCCGTGCGCCGCGTG
GTCGCCAACCCGCAGGACATCCACCGCTACACGGCGGAGTTCTTCGCGCTGGCCAAATCGGTGAAGGCGGCGCAGAAAGC
CGGCGGCGCGAGCGCGGGCAGCAGCTTCGAGCAGCTGGTGGAGCTGGGCCGCAGCAACAAGCAGCTGGACGCGAACGACC
AGGGCGTGGTCAAGGTGGTGGACTGGCTCTGGCAGTACGCCTTCGACCAGCGCGCGAGCGACATCCACCTGGAGCCGCGG
CGCGAGCAGGGCGTGATCCGCTTCCGGATCGACGGGGTGCTGCATCCGGTCTACCAGATGCCCATGGGCGTGCTGAACGC
CATGGTGTCGCGCGTGAAGCTGCTGGGCCGCATGGACGTGGTCGAGAAGCGCCGCCCGCAGGACGGCCGCATCAAGACCC
GCAACCCGCGCGGCGACGAGGTGGAAATGCGCCTCTCGACCCTGCCCACGGCCTTCGGCGAGAAGATGGTGATGCGGATA
TTCGACCCGGACAACACCGTCAAGGACCTGGACGCGCTGGGCTTCTCTCCGCACGACGCGCAGCGCTGGGAAGCGCTGGT
GCAGCGTCCGCACGGCATCATCCTGGTCACCGGGCCCACGGGCTCGGGCAAGACCACCACGCTCTACTCCACGCTCAAGC
GCCTGGCCACCGAAGAGGTGAACGTGAGCACGGTGGAGGACCCGATCGAGATGATCGAGCCGAGCTTCAACCAGACGCAG
GTGCAGCCGCAACTCGATTTCAGCTTCGCGGAAGGCCTGCGCGCGCTCATGCGGCAGGACCCGGACATCCTGATGGTGGG
CGAGATCCGCGACCTGGAGACGGCGGACATGGCCGTGCAGGCCGCGCTCACGGGCCACCTGGTGTTCTCCACGCTGCACA
CCAACGACGCCCCCTCGGCCATCAGCCGGCTGATGGAACTGGGCGTGCCGCCCTATCTCATCAACGCCACGCTGCTGGGC
GTGCTCGCGCAGCGGCTGGTGCGCACGCTCTGCCGGCAGTGCCGCCAGCGCGACGAGGCGGCCGGCATCGAACGCCTGGC
CGAGGTGGTGCGGCCCTGGAAGATCACCGGCGGCTACCACCCCTACCGGCCGGTGGGCTGCGTGGAATGCCGCATGACGG
GCTTCCACGGCCGCATGGGGCTCTACGAGCTGCTCACCGTGAGCGAGGCTCTGAAGGAGCGCATCACCCAGTCGCCCTCC
ATCGATGCGCTGCGGCGCCAGGCGCTGCAGGACGGCATGCGCCCGCTGCGGCTGGCCGGCGCGCTGCGCGTGGCCGAAGG
CGTGACCACCCTGGAGGAGGTCATCGCCGCAACGCCGCCGCTGGAGTCACGTGACCGGGCGGGCCCGCCGTCCGCGTCCT
GA

Upstream 100 bases:

>100_bases
CCGCGCGGACCTGCTGGCCCGCGCCGATGCGGTGCAGGCGCGGTGGGACCTGCCCGCGGCGAAGTGGCCGCGCGTGTTCA
AGCCGGTGGAGTGACGCGCG

Downstream 100 bases:

>100_bases
ATCCGTTTTCCGCCAGTCGAAAAACTGGAAAATGCCCGGCGCGCCGTGCCGGCATTTTCGCTGTGCGCGTTGCGGTTTTC
CCTAGGTGCAGGGTAGGTGG

Product: type II secretion system protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MSRSLPPTLHDAAGPAAPAGFAGPLEWRRIVEWLRADGVISQEEAARTIARCSQAESRQHPLVRLASVAMARASDGRPLD
LESLTEYLAGRSGLAYLRIDPLKVDVGRVADMMSASYAERHRVLPVQVTPREIVVATAEPFVDDWVAEVERQSRRAVRRV
VANPQDIHRYTAEFFALAKSVKAAQKAGGASAGSSFEQLVELGRSNKQLDANDQGVVKVVDWLWQYAFDQRASDIHLEPR
REQGVIRFRIDGVLHPVYQMPMGVLNAMVSRVKLLGRMDVVEKRRPQDGRIKTRNPRGDEVEMRLSTLPTAFGEKMVMRI
FDPDNTVKDLDALGFSPHDAQRWEALVQRPHGIILVTGPTGSGKTTTLYSTLKRLATEEVNVSTVEDPIEMIEPSFNQTQ
VQPQLDFSFAEGLRALMRQDPDILMVGEIRDLETADMAVQAALTGHLVFSTLHTNDAPSAISRLMELGVPPYLINATLLG
VLAQRLVRTLCRQCRQRDEAAGIERLAEVVRPWKITGGYHPYRPVGCVECRMTGFHGRMGLYELLTVSEALKERITQSPS
IDALRRQALQDGMRPLRLAGALRVAEGVTTLEEVIAATPPLESRDRAGPPSAS

Sequences:

>Translated_613_residues
MSRSLPPTLHDAAGPAAPAGFAGPLEWRRIVEWLRADGVISQEEAARTIARCSQAESRQHPLVRLASVAMARASDGRPLD
LESLTEYLAGRSGLAYLRIDPLKVDVGRVADMMSASYAERHRVLPVQVTPREIVVATAEPFVDDWVAEVERQSRRAVRRV
VANPQDIHRYTAEFFALAKSVKAAQKAGGASAGSSFEQLVELGRSNKQLDANDQGVVKVVDWLWQYAFDQRASDIHLEPR
REQGVIRFRIDGVLHPVYQMPMGVLNAMVSRVKLLGRMDVVEKRRPQDGRIKTRNPRGDEVEMRLSTLPTAFGEKMVMRI
FDPDNTVKDLDALGFSPHDAQRWEALVQRPHGIILVTGPTGSGKTTTLYSTLKRLATEEVNVSTVEDPIEMIEPSFNQTQ
VQPQLDFSFAEGLRALMRQDPDILMVGEIRDLETADMAVQAALTGHLVFSTLHTNDAPSAISRLMELGVPPYLINATLLG
VLAQRLVRTLCRQCRQRDEAAGIERLAEVVRPWKITGGYHPYRPVGCVECRMTGFHGRMGLYELLTVSEALKERITQSPS
IDALRRQALQDGMRPLRLAGALRVAEGVTTLEEVIAATPPLESRDRAGPPSAS
>Mature_612_residues
SRSLPPTLHDAAGPAAPAGFAGPLEWRRIVEWLRADGVISQEEAARTIARCSQAESRQHPLVRLASVAMARASDGRPLDL
ESLTEYLAGRSGLAYLRIDPLKVDVGRVADMMSASYAERHRVLPVQVTPREIVVATAEPFVDDWVAEVERQSRRAVRRVV
ANPQDIHRYTAEFFALAKSVKAAQKAGGASAGSSFEQLVELGRSNKQLDANDQGVVKVVDWLWQYAFDQRASDIHLEPRR
EQGVIRFRIDGVLHPVYQMPMGVLNAMVSRVKLLGRMDVVEKRRPQDGRIKTRNPRGDEVEMRLSTLPTAFGEKMVMRIF
DPDNTVKDLDALGFSPHDAQRWEALVQRPHGIILVTGPTGSGKTTTLYSTLKRLATEEVNVSTVEDPIEMIEPSFNQTQV
QPQLDFSFAEGLRALMRQDPDILMVGEIRDLETADMAVQAALTGHLVFSTLHTNDAPSAISRLMELGVPPYLINATLLGV
LAQRLVRTLCRQCRQRDEAAGIERLAEVVRPWKITGGYHPYRPVGCVECRMTGFHGRMGLYELLTVSEALKERITQSPSI
DALRRQALQDGMRPLRLAGALRVAEGVTTLEEVIAATPPLESRDRAGPPSAS

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=447, Percent_Identity=41.3870246085011, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI1786296, Length=371, Percent_Identity=43.9353099730458, Blast_Score=304, Evalue=9e-84,
Organism=Escherichia coli, GI87082188, Length=125, Percent_Identity=44, Blast_Score=102, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 67897; Mature: 67766

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSLPPTLHDAAGPAAPAGFAGPLEWRRIVEWLRADGVISQEEAARTIARCSQAESRQH
CCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
PLVRLASVAMARASDGRPLDLESLTEYLAGRSGLAYLRIDPLKVDVGRVADMMSASYAER
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEECCEEECHHHHHHHHHHHHHHH
HRVLPVQVTPREIVVATAEPFVDDWVAEVERQSRRAVRRVVANPQDIHRYTAEFFALAKS
CCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VKAAQKAGGASAGSSFEQLVELGRSNKQLDANDQGVVKVVDWLWQYAFDQRASDIHLEPR
HHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
REQGVIRFRIDGVLHPVYQMPMGVLNAMVSRVKLLGRMDVVEKRRPQDGRIKTRNPRGDE
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCH
VEMRLSTLPTAFGEKMVMRIFDPDNTVKDLDALGFSPHDAQRWEALVQRPHGIILVTGPT
HHHHHHHCCHHHHHHHEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCC
GSGKTTTLYSTLKRLATEEVNVSTVEDPIEMIEPSFNQTQVQPQLDFSFAEGLRALMRQD
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCC
PDILMVGEIRDLETADMAVQAALTGHLVFSTLHTNDAPSAISRLMELGVPPYLINATLLG
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHH
VLAQRLVRTLCRQCRQRDEAAGIERLAEVVRPWKITGGYHPYRPVGCVECRMTGFHGRMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCEEEEECCCCCCHH
LYELLTVSEALKERITQSPSIDALRRQALQDGMRPLRLAGALRVAEGVTTLEEVIAATPP
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
LESRDRAGPPSAS
CCCCCCCCCCCCC
>Mature Secondary Structure 
SRSLPPTLHDAAGPAAPAGFAGPLEWRRIVEWLRADGVISQEEAARTIARCSQAESRQH
CCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
PLVRLASVAMARASDGRPLDLESLTEYLAGRSGLAYLRIDPLKVDVGRVADMMSASYAER
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEECCEEECHHHHHHHHHHHHHHH
HRVLPVQVTPREIVVATAEPFVDDWVAEVERQSRRAVRRVVANPQDIHRYTAEFFALAKS
CCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VKAAQKAGGASAGSSFEQLVELGRSNKQLDANDQGVVKVVDWLWQYAFDQRASDIHLEPR
HHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
REQGVIRFRIDGVLHPVYQMPMGVLNAMVSRVKLLGRMDVVEKRRPQDGRIKTRNPRGDE
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCH
VEMRLSTLPTAFGEKMVMRIFDPDNTVKDLDALGFSPHDAQRWEALVQRPHGIILVTGPT
HHHHHHHCCHHHHHHHEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCC
GSGKTTTLYSTLKRLATEEVNVSTVEDPIEMIEPSFNQTQVQPQLDFSFAEGLRALMRQD
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCC
PDILMVGEIRDLETADMAVQAALTGHLVFSTLHTNDAPSAISRLMELGVPPYLINATLLG
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHH
VLAQRLVRTLCRQCRQRDEAAGIERLAEVVRPWKITGGYHPYRPVGCVECRMTGFHGRMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCEEEEECCCCCCHH
LYELLTVSEALKERITQSPSIDALRRQALQDGMRPLRLAGALRVAEGVTTLEEVIAATPP
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
LESRDRAGPPSAS
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]