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The map label for this gene is hisH [H]

Identifier: 120609726

GI number: 120609726

Start: 1131542

End: 1132210

Strand: Direct

Name: hisH [H]

Synonym: Aave_1033

Alternate gene names: 120609726

Gene position: 1131542-1132210 (Clockwise)

Preceding gene: 120609725

Following gene: 120609727

Centisome position: 21.14

GC content: 66.07

Gene sequence:

>669_bases
ATGAATGTTGAAGCAAAGACTGTCGCCGTCGTGGACTACGGCATGGGGAACCTGCGCTCGGTGTCCCAGGCCGTGCAGGC
CGCCGCCGAGGGCAGTGGCTGGACCGTGGTCGTGACCTCGCGCCCCGAGGACGTGCGTGCCGCGCAGCGCGTGGTGCTGC
CGGGGCAGGGCGCGATGCCCGACTGCATGCGCGAGCTGCGCGAATCCGGCCTGCAGGAATCCGTGCTGGAGGCTGCGGCC
TCCAAGCCGCTTTTCGGCGTCTGCGTGGGCATGCAGATGCTGCTGGACCACAGCGCCGAGGGCGACACCCCGGGCCTGGG
GCTGATTCCCGGCGACGTGGTGCGCTTCGAGTTGGCCGGCCGCCTGCAGCCGGACGGCAGTCGCTACAAGGTGCCCCAGA
TGGGCTGGAACCGGGTGCGGCAGATGCCGCACGGAGGGGCCGTGCATCCCGTGTGGGCCGGCATTCCTGACGAAAACTAT
TTTTATTTCGTGCATAGTTTCCATGCGTTGCCGCGCGACGCCGCGCACACTGTAGGGGAAACCGATTACGGCGGGCGCTT
TGCATCCGCCGTGGCTCGCGATAATATTTTTGCCACGCAATTCCATCCTGAGAAAAGTTCCGGGCACGGCCTCGCGCTCT
ACCGCAACTTCCTCCACTGGAATCCCTGA

Upstream 100 bases:

>100_bases
CTTCAAGGCCTTCGCGCGAGCGCTGCGCGCAGCGCTCGCGCGCGACCCGCGCTCGGCCGGCGTCATTCCCTCCACCAAGG
GCTCGCTCTAGGCTGCCACC

Downstream 100 bases:

>100_bases
GCAATCTCCCTCGCTTCCTATCCTCTTTCTTCCACCCGGCCGGCACTGGCACGCCCTGCCACGGCATTTCCCCACCTCGT
CATTCCATCATGCTGCTCAT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MNVEAKTVAVVDYGMGNLRSVSQAVQAAAEGSGWTVVVTSRPEDVRAAQRVVLPGQGAMPDCMRELRESGLQESVLEAAA
SKPLFGVCVGMQMLLDHSAEGDTPGLGLIPGDVVRFELAGRLQPDGSRYKVPQMGWNRVRQMPHGGAVHPVWAGIPDENY
FYFVHSFHALPRDAAHTVGETDYGGRFASAVARDNIFATQFHPEKSSGHGLALYRNFLHWNP

Sequences:

>Translated_222_residues
MNVEAKTVAVVDYGMGNLRSVSQAVQAAAEGSGWTVVVTSRPEDVRAAQRVVLPGQGAMPDCMRELRESGLQESVLEAAA
SKPLFGVCVGMQMLLDHSAEGDTPGLGLIPGDVVRFELAGRLQPDGSRYKVPQMGWNRVRQMPHGGAVHPVWAGIPDENY
FYFVHSFHALPRDAAHTVGETDYGGRFASAVARDNIFATQFHPEKSSGHGLALYRNFLHWNP
>Mature_222_residues
MNVEAKTVAVVDYGMGNLRSVSQAVQAAAEGSGWTVVVTSRPEDVRAAQRVVLPGQGAMPDCMRELRESGLQESVLEAAA
SKPLFGVCVGMQMLLDHSAEGDTPGLGLIPGDVVRFELAGRLQPDGSRYKVPQMGWNRVRQMPHGGAVHPVWAGIPDENY
FYFVHSFHALPRDAAHTVGETDYGGRFASAVARDNIFATQFHPEKSSGHGLALYRNFLHWNP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=214, Percent_Identity=36.4485981308411, Blast_Score=118, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6319725, Length=224, Percent_Identity=34.375, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 24112; Mature: 24112

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVEAKTVAVVDYGMGNLRSVSQAVQAAAEGSGWTVVVTSRPEDVRAAQRVVLPGQGAMP
CCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHEEECCCCCCCH
DCMRELRESGLQESVLEAAASKPLFGVCVGMQMLLDHSAEGDTPGLGLIPGDVVRFELAG
HHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECC
RLQPDGSRYKVPQMGWNRVRQMPHGGAVHPVWAGIPDENYFYFVHSFHALPRDAAHTVGE
CCCCCCCEEECCCCCHHHHHHCCCCCCCCCEECCCCCCCEEEEEEEHHCCCCHHHHHCCC
TDYGGRFASAVARDNIFATQFHPEKSSGHGLALYRNFLHWNP
CCCCCHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MNVEAKTVAVVDYGMGNLRSVSQAVQAAAEGSGWTVVVTSRPEDVRAAQRVVLPGQGAMP
CCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHEEECCCCCCCH
DCMRELRESGLQESVLEAAASKPLFGVCVGMQMLLDHSAEGDTPGLGLIPGDVVRFELAG
HHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECC
RLQPDGSRYKVPQMGWNRVRQMPHGGAVHPVWAGIPDENYFYFVHSFHALPRDAAHTVGE
CCCCCCCEEECCCCCHHHHHHCCCCCCCCCEECCCCCCCEEEEEEEHHCCCCHHHHHCCC
TDYGGRFASAVARDNIFATQFHPEKSSGHGLALYRNFLHWNP
CCCCCHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12754231 [H]