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The map label for this gene is fliY [H]

Identifier: 120609704

GI number: 120609704

Start: 1112374

End: 1113177

Strand: Direct

Name: fliY [H]

Synonym: Aave_1010

Alternate gene names: 120609704

Gene position: 1112374-1113177 (Clockwise)

Preceding gene: 120609703

Following gene: 120609705

Centisome position: 20.78

GC content: 65.55

Gene sequence:

>804_bases
ATGAAGAAGATCGCCGCCATCGCCCTGGTGTCCCTCGGTGCCCTGCTTGCCGCCTGCGGCAAGAACGACAGCGCCCAGCC
GGCCGCCTCCACCCCCGCCACGCCTGCGGCCGCCGTCACGAAGATCGTGATCGGCCTGGACGACAATTTCCCGCCGATGG
GCTTCCGCGACGACAAGAACCAGCTCGTGGGCTTCGACATCGACATGGCCCGCGAGGCCGCCAAGCGCATGGGCGTGGAG
GTGGAGTTCAAGCCGATCGACTGGAGCGCCAAGGAGGCCGAACTGTCGGGCAGGCGCGTGGACGCGCTCTGGAACGGCCT
CACGATCACCGAAGAGCGCAAGCAGAACATCGCCTTCACCGCGCCCTACATGGAGAACCACCAGATCATCGTGGTGGCGG
CCAACTCCCCGGTCAAGACCAAGGCGGACCTCGCCGGCAAGATCGTCGGCGCGCAGGAGGGCAGCAGCGCCGTGGACGCG
GTGAAGAAGGAAGAGGCCGTCTTCAAGTCGTTCAAGGAATTCAAGACCTTCGGCGACAACGTGACCGCGCTCATGGACCT
CTCCACCGGCCGCCTGGAAGCCGTGGTGGTGGACGAGGTGGTGGGCCGCTACTACGTCGCCAAGAAGCCGCAGGACTATG
CCGTGCTCGACGAGCATTTCGGTACCGAGGAATACGGCGTGGGCGTGCGCAAGGACGACACCGCGCTGCAGGGCCGCCTC
GACAAGGCGCTCGCCGACATGAAGCAGGACGGCGCCGCGGCCACGATCGCCACGCAGTGGTTTGGCAAGAACATCATCAA
ATAA

Upstream 100 bases:

>100_bases
CCGTTCGGCGCAGGCCCTGTGCGCTGTTGCCGGCCTGCCTTTCCCTTCACCCTTTTTCGCTCCATGCCGGCCTGCGGGCC
GGCTTTTCGTTGCCATTGCC

Downstream 100 bases:

>100_bases
CGGCGTTCATCCCCCTGAGGCGCTGCGCCTTCTCCCTTCTCCCGCATCGCTGCGCGATGCGGGAAGGGGGACGCAGCCGG
CGCGGCGGGGCGGCCCTTGC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKKIAAIALVSLGALLAACGKNDSAQPAASTPATPAAAVTKIVIGLDDNFPPMGFRDDKNQLVGFDIDMAREAAKRMGVE
VEFKPIDWSAKEAELSGRRVDALWNGLTITEERKQNIAFTAPYMENHQIIVVAANSPVKTKADLAGKIVGAQEGSSAVDA
VKKEEAVFKSFKEFKTFGDNVTALMDLSTGRLEAVVVDEVVGRYYVAKKPQDYAVLDEHFGTEEYGVGVRKDDTALQGRL
DKALADMKQDGAAATIATQWFGKNIIK

Sequences:

>Translated_267_residues
MKKIAAIALVSLGALLAACGKNDSAQPAASTPATPAAAVTKIVIGLDDNFPPMGFRDDKNQLVGFDIDMAREAAKRMGVE
VEFKPIDWSAKEAELSGRRVDALWNGLTITEERKQNIAFTAPYMENHQIIVVAANSPVKTKADLAGKIVGAQEGSSAVDA
VKKEEAVFKSFKEFKTFGDNVTALMDLSTGRLEAVVVDEVVGRYYVAKKPQDYAVLDEHFGTEEYGVGVRKDDTALQGRL
DKALADMKQDGAAATIATQWFGKNIIK
>Mature_267_residues
MKKIAAIALVSLGALLAACGKNDSAQPAASTPATPAAAVTKIVIGLDDNFPPMGFRDDKNQLVGFDIDMAREAAKRMGVE
VEFKPIDWSAKEAELSGRRVDALWNGLTITEERKQNIAFTAPYMENHQIIVVAANSPVKTKADLAGKIVGAQEGSSAVDA
VKKEEAVFKSFKEFKTFGDNVTALMDLSTGRLEAVVVDEVVGRYYVAKKPQDYAVLDEHFGTEEYGVGVRKDDTALQGRL
DKALADMKQDGAAATIATQWFGKNIIK

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=230, Percent_Identity=33.0434782608696, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787085, Length=231, Percent_Identity=33.7662337662338, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788649, Length=245, Percent_Identity=33.469387755102, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=34.061135371179, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787031, Length=228, Percent_Identity=31.140350877193, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787088, Length=235, Percent_Identity=31.063829787234, Blast_Score=106, Evalue=1e-24,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28769; Mature: 28769

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAAIALVSLGALLAACGKNDSAQPAASTPATPAAAVTKIVIGLDDNFPPMGFRDDKN
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCCCCCCCCC
QLVGFDIDMAREAAKRMGVEVEFKPIDWSAKEAELSGRRVDALWNGLTITEERKQNIAFT
CEEECCHHHHHHHHHHCCCEEEEECCCCCCCHHHCCCCEEHHHHCCCEEEHHHHCCEEEE
APYMENHQIIVVAANSPVKTKADLAGKIVGAQEGSSAVDAVKKEEAVFKSFKEFKTFGDN
CCCCCCCEEEEEECCCCCCCHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
VTALMDLSTGRLEAVVVDEVVGRYYVAKKPQDYAVLDEHFGTEEYGVGVRKDDTALQGRL
EEEEEECCCCCEEEEHHHHHHHHEEECCCCCCCCHHHHHCCCHHHCCCCCCCCHHHHHHH
DKALADMKQDGAAATIATQWFGKNIIK
HHHHHHHHCCCCEEEEHHHHHHHHCCC
>Mature Secondary Structure
MKKIAAIALVSLGALLAACGKNDSAQPAASTPATPAAAVTKIVIGLDDNFPPMGFRDDKN
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCCCCCCCCC
QLVGFDIDMAREAAKRMGVEVEFKPIDWSAKEAELSGRRVDALWNGLTITEERKQNIAFT
CEEECCHHHHHHHHHHCCCEEEEECCCCCCCHHHCCCCEEHHHHCCCEEEHHHHCCEEEE
APYMENHQIIVVAANSPVKTKADLAGKIVGAQEGSSAVDAVKKEEAVFKSFKEFKTFGDN
CCCCCCCEEEEEECCCCCCCHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
VTALMDLSTGRLEAVVVDEVVGRYYVAKKPQDYAVLDEHFGTEEYGVGVRKDDTALQGRL
EEEEEECCCCCEEEEHHHHHHHHEEECCCCCCCCHHHHHCCCHHHCCCCCCCCHHHHHHH
DKALADMKQDGAAATIATQWFGKNIIK
HHHHHHHHCCCCEEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]