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The map label for this gene is gltD [H]

Identifier: 120609703

GI number: 120609703

Start: 1110548

End: 1112026

Strand: Direct

Name: gltD [H]

Synonym: Aave_1009

Alternate gene names: 120609703

Gene position: 1110548-1112026 (Clockwise)

Preceding gene: 120609702

Following gene: 120609704

Centisome position: 20.75

GC content: 67.75

Gene sequence:

>1479_bases
ATGGGAAAGACCACCGGCTTCATGGAATATGAGCGCATCGAGGAGGGCTACAAGCCCGTCGCCGAGCGCCTGAAGCACTA
CAAGGAATTCGTCATCGGCCTCGATGACGCGCAGGCCAAGCTGCAGGGCGCGCGCTGCATGGACTGTGGCACTCCGTTCT
GCAACAACGGCTGCCCGGTCAACAACATCATTCCGGACTTCAACGACCTGGTCTACCAGCAGGACTGGAAGAGCGCCTGG
GCCGTGCTGGACTCGACCAACAACTTCCCCGAGTTCACCGGCCGCATCTGTCCCGCGCCCTGCGAGGCCGCCTGCGTGCT
GAACGTGAATGACGACCCGGTGGGCATCAAGTCCATCGAGCACGCCATCATCGACCGCGCCTGGGCCGAGGGCTGGGTGA
AGCCGCGCCCGGCCAAGCACAGGACCGGCAAGAAAGTGGCCGTGGTGGGCTCCGGCCCCGCGGGCATGGCGGCCGCCCAG
CAGCTCGCGCGCGCCGGCCACGACGTGACGCTCTTCGAGAAGAACGACCGCATCGGTGGCCTGCTGCGCTACGGCATTCC
CGACTTCAAGATGGAGAAGTCGCACATCGACCGCCGCGCCGAGCAGATGAAGGCCGAAGGCGTGCAGATCCGCACCGGCG
TGCTCGTCGGCACGGCCAAGGACGGTCTGGGCAAGGGCTCCAAGGTCACCAACTGGGCCAGGGAAACCGTGACGCCCGAG
CAACTGCAGGCCGAGTTCGACGCCGTGCTGCTGGCCGGTGGCGCCGAGCAGAGCCGCGACCTGCCCGTGCCGGGCCGCGA
GCTGGACGGCGTGCATTTCGCGATGGAGTTCCTGCCCCAGCAGAACAAGGTGAATGCGGGCGACAAGCTCAAGGGCCAGA
TCCGCGCGGACGGCAAGCACGTCATCGTGATCGGCGGCGGCGACACGGGCAGCGATTGCGTGGGCACGAGCAACCGCCAC
GGTGCCGCGAGCGTGACGCAGTTCGAGCTGATGCCCCAGCCGCCCGAGGTGGAAAACCGCCCGCTCACCTGGCCCTACTG
GCCCTACAAGCTGCGCACCAGCTCGAGCCACGAAGAGGGCTGCGAGCGCGAGTTCGCCATCGCCACGAAGGAATTCATCG
GCGAGAAGGGCAAGGTCACGGGCCTGAAGACCGTGCGCGTCGAATGGAAGGACGGCAAGATGGTCGAGGTGGCCGGCTCC
GAGCAGACGCTCAAGGCCGACCTCGTGCTGCTGGCCATGGGCTTCGTGAGCCCCGTGTCGCCGGTGCTGGAGGCCTTCGG
CGTCGAGAAGGACGCGCGCGGCAACGCCCGCGCCACGACGGAGTTCACCGGCGGCTACGCGACCAACGTGCCCAAGGTAT
TCGCTGCCGGCGACATGCGCCGCGGCCAGTCCCTGGTGGTGTGGGCCATCCGCGAAGGCCGGCAGGCCGCGCGGGCGGTG
GACGAGTTCCTGATGGGTTACTCCGACCTGCCGCGCTGA

Upstream 100 bases:

>100_bases
CCCGAGCAGGAAAAGGCGCGCGCCGCGGTGCCGGCCAGGTAAGGCCGGATCGAACCGACGACGCACCGCTTCGCACCCCA
CAACGACAGGACACGCAATC

Downstream 100 bases:

>100_bases
CGCGCAGCCCCCCGTGCCCGGCCCGCCAGCGGGCCGCCCGGCATGCCGACCGCGCCCCGGGCGCCGTTTTTCATGGTGGG
GCATGCGGGTTTTGCTATCT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MGKTTGFMEYERIEEGYKPVAERLKHYKEFVIGLDDAQAKLQGARCMDCGTPFCNNGCPVNNIIPDFNDLVYQQDWKSAW
AVLDSTNNFPEFTGRICPAPCEAACVLNVNDDPVGIKSIEHAIIDRAWAEGWVKPRPAKHRTGKKVAVVGSGPAGMAAAQ
QLARAGHDVTLFEKNDRIGGLLRYGIPDFKMEKSHIDRRAEQMKAEGVQIRTGVLVGTAKDGLGKGSKVTNWARETVTPE
QLQAEFDAVLLAGGAEQSRDLPVPGRELDGVHFAMEFLPQQNKVNAGDKLKGQIRADGKHVIVIGGGDTGSDCVGTSNRH
GAASVTQFELMPQPPEVENRPLTWPYWPYKLRTSSSHEEGCEREFAIATKEFIGEKGKVTGLKTVRVEWKDGKMVEVAGS
EQTLKADLVLLAMGFVSPVSPVLEAFGVEKDARGNARATTEFTGGYATNVPKVFAAGDMRRGQSLVVWAIREGRQAARAV
DEFLMGYSDLPR

Sequences:

>Translated_492_residues
MGKTTGFMEYERIEEGYKPVAERLKHYKEFVIGLDDAQAKLQGARCMDCGTPFCNNGCPVNNIIPDFNDLVYQQDWKSAW
AVLDSTNNFPEFTGRICPAPCEAACVLNVNDDPVGIKSIEHAIIDRAWAEGWVKPRPAKHRTGKKVAVVGSGPAGMAAAQ
QLARAGHDVTLFEKNDRIGGLLRYGIPDFKMEKSHIDRRAEQMKAEGVQIRTGVLVGTAKDGLGKGSKVTNWARETVTPE
QLQAEFDAVLLAGGAEQSRDLPVPGRELDGVHFAMEFLPQQNKVNAGDKLKGQIRADGKHVIVIGGGDTGSDCVGTSNRH
GAASVTQFELMPQPPEVENRPLTWPYWPYKLRTSSSHEEGCEREFAIATKEFIGEKGKVTGLKTVRVEWKDGKMVEVAGS
EQTLKADLVLLAMGFVSPVSPVLEAFGVEKDARGNARATTEFTGGYATNVPKVFAAGDMRRGQSLVVWAIREGRQAARAV
DEFLMGYSDLPR
>Mature_491_residues
GKTTGFMEYERIEEGYKPVAERLKHYKEFVIGLDDAQAKLQGARCMDCGTPFCNNGCPVNNIIPDFNDLVYQQDWKSAWA
VLDSTNNFPEFTGRICPAPCEAACVLNVNDDPVGIKSIEHAIIDRAWAEGWVKPRPAKHRTGKKVAVVGSGPAGMAAAQQ
LARAGHDVTLFEKNDRIGGLLRYGIPDFKMEKSHIDRRAEQMKAEGVQIRTGVLVGTAKDGLGKGSKVTNWARETVTPEQ
LQAEFDAVLLAGGAEQSRDLPVPGRELDGVHFAMEFLPQQNKVNAGDKLKGQIRADGKHVIVIGGGDTGSDCVGTSNRHG
AASVTQFELMPQPPEVENRPLTWPYWPYKLRTSSSHEEGCEREFAIATKEFIGEKGKVTGLKTVRVEWKDGKMVEVAGSE
QTLKADLVLLAMGFVSPVSPVLEAFGVEKDARGNARATTEFTGGYATNVPKVFAAGDMRRGQSLVVWAIREGRQAARAVD
EFLMGYSDLPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=470, Percent_Identity=25.9574468085106, Blast_Score=110, Evalue=4e-24,
Organism=Escherichia coli, GI1789606, Length=490, Percent_Identity=35.1020408163265, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1788811, Length=471, Percent_Identity=34.6072186836518, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI87082180, Length=474, Percent_Identity=33.1223628691983, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI1788468, Length=449, Percent_Identity=28.9532293986637, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789243, Length=445, Percent_Identity=26.9662921348315, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17570289, Length=511, Percent_Identity=43.0528375733855, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI71984108, Length=478, Percent_Identity=25.1046025104602, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320030, Length=505, Percent_Identity=46.9306930693069, Blast_Score=452, Evalue=1e-128,
Organism=Drosophila melanogaster, GI28574881, Length=507, Percent_Identity=50.4930966469428, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665539, Length=507, Percent_Identity=50.4930966469428, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665547, Length=507, Percent_Identity=50.4930966469428, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24665543, Length=507, Percent_Identity=50.4930966469428, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24640763, Length=474, Percent_Identity=24.6835443037975, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI18858217, Length=474, Percent_Identity=24.6835443037975, Blast_Score=105, Evalue=9e-23,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53749; Mature: 53617

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTTGFMEYERIEEGYKPVAERLKHYKEFVIGLDDAQAKLQGARCMDCGTPFCNNGCPV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHCCCEEECCCCCCCCCCCCC
NNIIPDFNDLVYQQDWKSAWAVLDSTNNFPEFTGRICPAPCEAACVLNVNDDPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCEEEEEECCCCCCCHHHHH
HAIIDRAWAEGWVKPRPAKHRTGKKVAVVGSGPAGMAAAQQLARAGHDVTLFEKNDRIGG
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKMEKSHIDRRAEQMKAEGVQIRTGVLVGTAKDGLGKGSKVTNWARETVTPE
EEECCCCCCEECHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCCHHHHHHHCCCHH
QLQAEFDAVLLAGGAEQSRDLPVPGRELDGVHFAMEFLPQQNKVNAGDKLKGQIRADGKH
HHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEECCCE
VIVIGGGDTGSDCVGTSNRHGAASVTQFELMPQPPEVENRPLTWPYWPYKLRTSSSHEEG
EEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCHHH
CEREFAIATKEFIGEKGKVTGLKTVRVEWKDGKMVEVAGSEQTLKADLVLLAMGFVSPVS
CCHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
PVLEAFGVEKDARGNARATTEFTGGYATNVPKVFAAGDMRRGQSLVVWAIREGRQAARAV
HHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCEEEEEEEHHCHHHHHHH
DEFLMGYSDLPR
HHHHHCCCCCCC
>Mature Secondary Structure 
GKTTGFMEYERIEEGYKPVAERLKHYKEFVIGLDDAQAKLQGARCMDCGTPFCNNGCPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHCCCEEECCCCCCCCCCCCC
NNIIPDFNDLVYQQDWKSAWAVLDSTNNFPEFTGRICPAPCEAACVLNVNDDPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCEEEEEECCCCCCCHHHHH
HAIIDRAWAEGWVKPRPAKHRTGKKVAVVGSGPAGMAAAQQLARAGHDVTLFEKNDRIGG
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKMEKSHIDRRAEQMKAEGVQIRTGVLVGTAKDGLGKGSKVTNWARETVTPE
EEECCCCCCEECHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCCHHHHHHHCCCHH
QLQAEFDAVLLAGGAEQSRDLPVPGRELDGVHFAMEFLPQQNKVNAGDKLKGQIRADGKH
HHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEECCCE
VIVIGGGDTGSDCVGTSNRHGAASVTQFELMPQPPEVENRPLTWPYWPYKLRTSSSHEEG
EEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCHHH
CEREFAIATKEFIGEKGKVTGLKTVRVEWKDGKMVEVAGSEQTLKADLVLLAMGFVSPVS
CCHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
PVLEAFGVEKDARGNARATTEFTGGYATNVPKVFAAGDMRRGQSLVVWAIREGRQAARAV
HHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCEEEEEEEHHCHHHHHHH
DEFLMGYSDLPR
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]