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The map label for this gene is metK [H]

Identifier: 120609673

GI number: 120609673

Start: 1074906

End: 1076087

Strand: Reverse

Name: metK [H]

Synonym: Aave_0979

Alternate gene names: 120609673

Gene position: 1076087-1074906 (Counterclockwise)

Preceding gene: 120609676

Following gene: 120609672

Centisome position: 20.1

GC content: 67.09

Gene sequence:

>1182_bases
ATGGCGAACGACTTTCTCTTCACGTCGGAATCGGTGTCCGAAGGCCACCCCGACAAGGTGGCGGACCAGATCTCCGACGC
CATCCTCGACGCGATCTTCCGCGAGGATCCCCGCAGCCGCGTGGCCGCCGAAACGCTGACCAACACCGGCCTCGTCGTGC
TGGCCGGCGAGATCACCACCAATGCCCACGTGGATTACATCCAGGTGGCGCGCGACACCATCAAGCGCATCGGCTACGAC
AACACCGACTACGGCATCGATTACAAGGGCTGCGCGGTGCTGGTGGCCTACGACAAGCAGAGCAACGACATCGCCCAGGG
CGTGGACCATGCCAGCGACGACCACCTGAACACCGGCGCCGGCGACCAGGGCCTGATGTTCGGCTACGCCTGCGACGAAA
CGCCCGAGCTGATGCCCGCGCCCATCTACTACGCGCACCGCCTGGTCGAGCGCCAGGCCCAGTTGCGCAAGGACGGCCGC
CTGCCCTTCCTGCGCCCGGACGCCAAGAGCCAGGTGACGATGCGCTACGTGGACGGCAAGCCCCACAGCATCGATACCGT
GGTGCTGTCCACCCAGCACCATCCCGACCAGTCCGAGACCGCCACCAGGATGAAGGCCAGCTTCATCGAGGCGGTGATCG
AGGAGATCATCAAGCCCGTGCTGCCCAAGGAGTGGCTGCAGGAGACGAAGTACCTGATCAACCCCACGGGCCGCTTCGTC
ATCGGCGGCCCGCAGGGCGACTGCGGCCTGACGGGCCGCAAGATCATCGTCGATACCTACGGCGGCGCCTGCCCCCACGG
CGGCGGCGCCTTCAGCGGCAAGGACCCGACGAAGGTGGACCGCTCGGCCGCCTACGCCGCGCGCTACGTGGCCAAGAACA
TCGTGGCCGCGGGCCTGGCGCGCCAGTGCCAGATCCAGGTGAGCTATGCCATCGGCGTGGCCCGCCCGATCAACGTCACC
GTCTACACCGAGGGCACGGGCGTGATCCCGGACGAGAAGATCGCCGCGCTGGTGCAGGAGCATTTCGACCTGCGGCCCAA
GGGCATCATCCAGATGCTCGACCTGCTGCGCCCGATCTACGAAAAGACCGCCGCCTACGGCCACTTCGGCCGCGAGGAAC
CCGAGTTCACCTGGGAAAAGACGGACAAGGCGGCCGCGCTGCGCGCAGCGGCCGGGCTGTAA

Upstream 100 bases:

>100_bases
TTGCAGGGCGACGTTAAACAACTCTGCTAAAGCGTTCGCCACGGCTCCACAAGGCTGAGGGCAACGCCCAAAATGCCAAA
AAGCACAGGAGTTTATTCAA

Downstream 100 bases:

>100_bases
GCCCGGCAACCCGCGCAGCGGGTTCGGCCGCCTTGCGGCGCAGGCTCACATCGCGCCAGCGATGTGAAGGCCCATAGGGC
CGTGCCGGAGCCGCAAGCCT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MANDFLFTSESVSEGHPDKVADQISDAILDAIFREDPRSRVAAETLTNTGLVVLAGEITTNAHVDYIQVARDTIKRIGYD
NTDYGIDYKGCAVLVAYDKQSNDIAQGVDHASDDHLNTGAGDQGLMFGYACDETPELMPAPIYYAHRLVERQAQLRKDGR
LPFLRPDAKSQVTMRYVDGKPHSIDTVVLSTQHHPDQSETATRMKASFIEAVIEEIIKPVLPKEWLQETKYLINPTGRFV
IGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLARQCQIQVSYAIGVARPINVT
VYTEGTGVIPDEKIAALVQEHFDLRPKGIIQMLDLLRPIYEKTAAYGHFGREEPEFTWEKTDKAAALRAAAGL

Sequences:

>Translated_393_residues
MANDFLFTSESVSEGHPDKVADQISDAILDAIFREDPRSRVAAETLTNTGLVVLAGEITTNAHVDYIQVARDTIKRIGYD
NTDYGIDYKGCAVLVAYDKQSNDIAQGVDHASDDHLNTGAGDQGLMFGYACDETPELMPAPIYYAHRLVERQAQLRKDGR
LPFLRPDAKSQVTMRYVDGKPHSIDTVVLSTQHHPDQSETATRMKASFIEAVIEEIIKPVLPKEWLQETKYLINPTGRFV
IGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLARQCQIQVSYAIGVARPINVT
VYTEGTGVIPDEKIAALVQEHFDLRPKGIIQMLDLLRPIYEKTAAYGHFGREEPEFTWEKTDKAAALRAAAGL
>Mature_392_residues
ANDFLFTSESVSEGHPDKVADQISDAILDAIFREDPRSRVAAETLTNTGLVVLAGEITTNAHVDYIQVARDTIKRIGYDN
TDYGIDYKGCAVLVAYDKQSNDIAQGVDHASDDHLNTGAGDQGLMFGYACDETPELMPAPIYYAHRLVERQAQLRKDGRL
PFLRPDAKSQVTMRYVDGKPHSIDTVVLSTQHHPDQSETATRMKASFIEAVIEEIIKPVLPKEWLQETKYLINPTGRFVI
GGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLARQCQIQVSYAIGVARPINVTV
YTEGTGVIPDEKIAALVQEHFDLRPKGIIQMLDLLRPIYEKTAAYGHFGREEPEFTWEKTDKAAALRAAAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=56.8421052631579, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=55.7894736842105, Blast_Score=401, Evalue=1e-112,
Organism=Escherichia coli, GI1789311, Length=389, Percent_Identity=68.3804627249357, Blast_Score=524, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17538498, Length=381, Percent_Identity=55.9055118110236, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25145633, Length=386, Percent_Identity=55.1813471502591, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17538494, Length=381, Percent_Identity=55.3805774278215, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17551082, Length=385, Percent_Identity=54.5454545454545, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI32565911, Length=335, Percent_Identity=53.7313432835821, Blast_Score=334, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI32565905, Length=381, Percent_Identity=49.6062992125984, Blast_Score=330, Evalue=9e-91,
Organism=Caenorhabditis elegans, GI25145635, Length=338, Percent_Identity=52.9585798816568, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI32565903, Length=308, Percent_Identity=52.9220779220779, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=59.5238095238095, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6323209, Length=385, Percent_Identity=57.1428571428571, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6320710, Length=384, Percent_Identity=56.5104166666667, Blast_Score=406, Evalue=1e-114,
Organism=Drosophila melanogaster, GI45552159, Length=381, Percent_Identity=51.9685039370079, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580529, Length=381, Percent_Identity=51.9685039370079, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580537, Length=381, Percent_Identity=51.9685039370079, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580533, Length=381, Percent_Identity=51.9685039370079, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580535, Length=381, Percent_Identity=51.9685039370079, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580531, Length=381, Percent_Identity=51.9685039370079, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580527, Length=381, Percent_Identity=51.4435695538058, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24580525, Length=381, Percent_Identity=51.4435695538058, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24580541, Length=382, Percent_Identity=48.4293193717278, Blast_Score=334, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=55.4621848739496, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42982; Mature: 42851

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDFLFTSESVSEGHPDKVADQISDAILDAIFREDPRSRVAAETLTNTGLVVLAGEITT
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCC
NAHVDYIQVARDTIKRIGYDNTDYGIDYKGCAVLVAYDKQSNDIAQGVDHASDDHLNTGA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHCCCCCCCCCCCCC
GDQGLMFGYACDETPELMPAPIYYAHRLVERQAQLRKDGRLPFLRPDAKSQVTMRYVDGK
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEECCC
PHSIDTVVLSTQHHPDQSETATRMKASFIEAVIEEIIKPVLPKEWLQETKYLINPTGRFV
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCEEECCCCCEE
IGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLA
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
RQCQIQVSYAIGVARPINVTVYTEGTGVIPDEKIAALVQEHFDLRPKGIIQMLDLLRPIY
HHEEEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
EKTAAYGHFGREEPEFTWEKTDKAAALRAAAGL
HHHHHHCCCCCCCCCCCHHHCHHHHHHHHHCCC
>Mature Secondary Structure 
ANDFLFTSESVSEGHPDKVADQISDAILDAIFREDPRSRVAAETLTNTGLVVLAGEITT
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCC
NAHVDYIQVARDTIKRIGYDNTDYGIDYKGCAVLVAYDKQSNDIAQGVDHASDDHLNTGA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHCCCCCCCCCCCCC
GDQGLMFGYACDETPELMPAPIYYAHRLVERQAQLRKDGRLPFLRPDAKSQVTMRYVDGK
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEECCC
PHSIDTVVLSTQHHPDQSETATRMKASFIEAVIEEIIKPVLPKEWLQETKYLINPTGRFV
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCEEECCCCCEE
IGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLA
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
RQCQIQVSYAIGVARPINVTVYTEGTGVIPDEKIAALVQEHFDLRPKGIIQMLDLLRPIY
HHEEEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
EKTAAYGHFGREEPEFTWEKTDKAAALRAAAGL
HHHHHHCCCCCCCCCCCHHHCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA