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The map label for this gene is pucI [H]

Identifier: 120609662

GI number: 120609662

Start: 1062067

End: 1063536

Strand: Direct

Name: pucI [H]

Synonym: Aave_0968

Alternate gene names: 120609662

Gene position: 1062067-1063536 (Clockwise)

Preceding gene: 120609661

Following gene: 120609663

Centisome position: 19.84

GC content: 67.35

Gene sequence:

>1470_bases
ATGATGAACAGTCCAGGCGGCGCGGCACCGATGGCAACCGCAACCACCACCTTTCCGGGCGGGCAGGACGCATCGCTCGC
GAACGAAGACCTGCTGCCCACCACCGCTGCGCAAAGACACTGGGCCTGGAAGGACTATGCCGCCCTGTGGATCGGCATGG
TGGTCTGCGTGCCCACGTACACCATGGCCAGCTCCATGCTGGACCAGGGCTTTTCCTGGCAGATGGCCGTGTGGCTGGTG
TTCCTGGCCAACTGCATCGTGCTGGTGCCCATGGTGCTGATCGGCCGCGTGGGCCCGAAGTACGGCGTGCCATTCCCGGT
GCTGGCGCGGGCCCCGTTCGGCGTCAAGGGCGCCAACCTGCCGGCGGTGCTGCGCGGACTGGTGGCCTGCGGCTGGTTTT
CCATCAACTGCTATTTCGGGGCGCTGGCGCTGCACGGCTTCCTGAACATCCTGGGCCTGGGGCTCCCGGGGCCTGCCGAG
GGGCAGACCATCAGCACCTCGCAGTTCGTGTGCTTCCTGGCCTTCTGGGCCGTGCACATCTGGTTCATCTGGAAGGGGCC
GGAGTCGATCCGCAAGCTGGAGGTGATCGCGGCGCCGCTGATGATCGCGGCGTCCGTCCTGCTGGTGGTCGTGCTGCTGG
TGCGCGTGCCGCCCAGCCAGCTGTTCAACCTGCCCGCCAAGGTAGTGGAGGGCGGGCCCAGGACATGGGCCGCGCTGACC
GGCCTGGTAGGGTTCTGGGCCACGCTGGCGCTGAACATTCCCGACTTCACGCGCTTCGCCCGCTCGCAGAAGGACCAGGT
GCTGGGCCAGGCCATCGGCCTGCCCATTCCCATGGCGCTGTTCTCGATGGTGGGCGTGATCGGCTTCTCGGCATCGGCCA
TCCTGTATGGCAAGGCGCAGCTGTTTCCGGACGGCCTGCTCACGCAGATGGGCAGCGTGGCCGCCGGGCTGGGCCTGCTG
GTGATCCTGCTGGCCAACCTGACGACGAACGTGGCGGCCAACCTGGTGTCGCCCTCCTATGATTTCAGCCAGATCGCGCC
GTCGAAGATCAGCTTCCGCATGGGCGGCGTGATCGCCGCGCTGCTCGGCCTGCTGTTCATGCCCTGGAAGCTGCTGGCCA
CGTCCGGAAGCTACCTCTTCGTCTGGCTGGGCGGCTACGGCACGCTGCTGGGCGCCATCGCCGGCATCCTGCTGGTCGAT
TACTACCTCGTGCGCAAGACCCAGCTGAAGGTGGAAGACCTCTACCGCCGCGGCGGCGACTACGAATACACCAACGGCTG
GAACGTGCACGCGCTCGTGGCTTTCCTGCTCGGCGTGCTGCCGTGCCTGCCGGGCTACCTGGCCGTGTCGGGCGTGGTGG
ACAAGGCCGGCATGCCCGCCATCTGGCTCACGCTGTTCGACTCGGGCTGGTTCTTCAGCCTCGCCGTGGCAGGCCTGTAC
TACTTCGCGACCGCCCGGAGGCCGCGGTAG

Upstream 100 bases:

>100_bases
CTGGACCACGCATCCCAACAACCCGCAGCGCGTCGCTACGGCCACGGCCACGGTCGCCGCCTGACGTCATCCATCCAGCC
CCTCATTCAGGAGTGACGAC

Downstream 100 bases:

>100_bases
CGGCCCCGCCGGCCCGCCGCGGCGCAGCGCTCTGCCCGCGGCCGGGCACTTTCCTTCCAGAGACAAGGAGAACCCATGAC
CCCCAGCCTTCTGATCCGCG

Product: cytosine/purines, uracil, thiamine, allantoin permease

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MMNSPGGAAPMATATTTFPGGQDASLANEDLLPTTAAQRHWAWKDYAALWIGMVVCVPTYTMASSMLDQGFSWQMAVWLV
FLANCIVLVPMVLIGRVGPKYGVPFPVLARAPFGVKGANLPAVLRGLVACGWFSINCYFGALALHGFLNILGLGLPGPAE
GQTISTSQFVCFLAFWAVHIWFIWKGPESIRKLEVIAAPLMIAASVLLVVVLLVRVPPSQLFNLPAKVVEGGPRTWAALT
GLVGFWATLALNIPDFTRFARSQKDQVLGQAIGLPIPMALFSMVGVIGFSASAILYGKAQLFPDGLLTQMGSVAAGLGLL
VILLANLTTNVAANLVSPSYDFSQIAPSKISFRMGGVIAALLGLLFMPWKLLATSGSYLFVWLGGYGTLLGAIAGILLVD
YYLVRKTQLKVEDLYRRGGDYEYTNGWNVHALVAFLLGVLPCLPGYLAVSGVVDKAGMPAIWLTLFDSGWFFSLAVAGLY
YFATARRPR

Sequences:

>Translated_489_residues
MMNSPGGAAPMATATTTFPGGQDASLANEDLLPTTAAQRHWAWKDYAALWIGMVVCVPTYTMASSMLDQGFSWQMAVWLV
FLANCIVLVPMVLIGRVGPKYGVPFPVLARAPFGVKGANLPAVLRGLVACGWFSINCYFGALALHGFLNILGLGLPGPAE
GQTISTSQFVCFLAFWAVHIWFIWKGPESIRKLEVIAAPLMIAASVLLVVVLLVRVPPSQLFNLPAKVVEGGPRTWAALT
GLVGFWATLALNIPDFTRFARSQKDQVLGQAIGLPIPMALFSMVGVIGFSASAILYGKAQLFPDGLLTQMGSVAAGLGLL
VILLANLTTNVAANLVSPSYDFSQIAPSKISFRMGGVIAALLGLLFMPWKLLATSGSYLFVWLGGYGTLLGAIAGILLVD
YYLVRKTQLKVEDLYRRGGDYEYTNGWNVHALVAFLLGVLPCLPGYLAVSGVVDKAGMPAIWLTLFDSGWFFSLAVAGLY
YFATARRPR
>Mature_489_residues
MMNSPGGAAPMATATTTFPGGQDASLANEDLLPTTAAQRHWAWKDYAALWIGMVVCVPTYTMASSMLDQGFSWQMAVWLV
FLANCIVLVPMVLIGRVGPKYGVPFPVLARAPFGVKGANLPAVLRGLVACGWFSINCYFGALALHGFLNILGLGLPGPAE
GQTISTSQFVCFLAFWAVHIWFIWKGPESIRKLEVIAAPLMIAASVLLVVVLLVRVPPSQLFNLPAKVVEGGPRTWAALT
GLVGFWATLALNIPDFTRFARSQKDQVLGQAIGLPIPMALFSMVGVIGFSASAILYGKAQLFPDGLLTQMGSVAAGLGLL
VILLANLTTNVAANLVSPSYDFSQIAPSKISFRMGGVIAALLGLLFMPWKLLATSGSYLFVWLGGYGTLLGAIAGILLVD
YYLVRKTQLKVEDLYRRGGDYEYTNGWNVHALVAFLLGVLPCLPGYLAVSGVVDKAGMPAIWLTLFDSGWFFSLAVAGLY
YFATARRPR

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=441, Percent_Identity=32.4263038548753, Blast_Score=196, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6319495, Length=460, Percent_Identity=30.4347826086957, Blast_Score=196, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6322219, Length=451, Percent_Identity=29.490022172949, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6319429, Length=495, Percent_Identity=27.6767676767677, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324645, Length=455, Percent_Identity=23.956043956044, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323266, Length=455, Percent_Identity=23.7362637362637, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324766, Length=437, Percent_Identity=23.3409610983982, Blast_Score=115, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 52412; Mature: 52412

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNSPGGAAPMATATTTFPGGQDASLANEDLLPTTAAQRHWAWKDYAALWIGMVVCVPTY
CCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHH
TMASSMLDQGFSWQMAVWLVFLANCIVLVPMVLIGRVGPKYGVPFPVLARAPFGVKGANL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCH
PAVLRGLVACGWFSINCYFGALALHGFLNILGLGLPGPAEGQTISTSQFVCFLAFWAVHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHHHH
WFIWKGPESIRKLEVIAAPLMIAASVLLVVVLLVRVPPSQLFNLPAKVVEGGPRTWAALT
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHCCCCHHHHHHH
GLVGFWATLALNIPDFTRFARSQKDQVLGQAIGLPIPMALFSMVGVIGFSASAILYGKAQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECHH
LFPDGLLTQMGSVAAGLGLLVILLANLTTNVAANLVSPSYDFSQIAPSKISFRMGGVIAA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHH
LLGLLFMPWKLLATSGSYLFVWLGGYGTLLGAIAGILLVDYYLVRKTQLKVEDLYRRGGD
HHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YEYTNGWNVHALVAFLLGVLPCLPGYLAVSGVVDKAGMPAIWLTLFDSGWFFSLAVAGLY
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEHHCCCHHHHHHHHHHH
YFATARRPR
HHHHCCCCC
>Mature Secondary Structure
MMNSPGGAAPMATATTTFPGGQDASLANEDLLPTTAAQRHWAWKDYAALWIGMVVCVPTY
CCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHH
TMASSMLDQGFSWQMAVWLVFLANCIVLVPMVLIGRVGPKYGVPFPVLARAPFGVKGANL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCH
PAVLRGLVACGWFSINCYFGALALHGFLNILGLGLPGPAEGQTISTSQFVCFLAFWAVHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHHHH
WFIWKGPESIRKLEVIAAPLMIAASVLLVVVLLVRVPPSQLFNLPAKVVEGGPRTWAALT
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHCCCCHHHHHHH
GLVGFWATLALNIPDFTRFARSQKDQVLGQAIGLPIPMALFSMVGVIGFSASAILYGKAQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECHH
LFPDGLLTQMGSVAAGLGLLVILLANLTTNVAANLVSPSYDFSQIAPSKISFRMGGVIAA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHH
LLGLLFMPWKLLATSGSYLFVWLGGYGTLLGAIAGILLVDYYLVRKTQLKVEDLYRRGGD
HHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YEYTNGWNVHALVAFLLGVLPCLPGYLAVSGVVDKAGMPAIWLTLFDSGWFFSLAVAGLY
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEHHCCCHHHHHHHHHHH
YFATARRPR
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]