Click here to switch to the map view.

The map label for this gene is asnB [H]

Identifier: 120609655

GI number: 120609655

Start: 1049802

End: 1051688

Strand: Direct

Name: asnB [H]

Synonym: Aave_0961

Alternate gene names: 120609655

Gene position: 1049802-1051688 (Clockwise)

Preceding gene: 120609654

Following gene: 120609656

Centisome position: 19.61

GC content: 61.47

Gene sequence:

>1887_bases
ATGTGCGGTATCGTCGGTGTGTTTTCCTCTGGAGATGACCCCGGCTCGGCTGGGCGGCCGGAGCGTTTTCTCAAGCTTCT
GCAGCACCGGGGGCCTGATCAGCAGGCCTACGTTCGCATGCAGCACGGGGAGGGCCGCGGCTGGCTGGGTCATACGCGCC
TCAAGGTCATCGATCTTTCCGATGCCGCAGGCCAGCCCATGTGGAGCGCGGATGGGCGCTATGCGCTCCTGTTCAACGGC
GAAATCTACAACTATCGCGAATTGCGGCAGGAGCTTCGCATCAAGGGTACGCATTTCCGTACCGATTCGGATTCCGAGGT
GCTGCTGCACGCCTGGCTTGCCTGGGGGCCGGATGTGCTTCCACGACTCGTCGGTATGTTTGCCTTCGTCATATACGACA
GGGAAATGGCCACCTTGCACGGCGCGCGCGATGCTTTCGGCATCAAGCCCCTGTACTACCGCTGCGGCGGTAGGGACTTT
AGTTTCGCCTCCGAGAGTTCGGTGCTTAGCTCCATGGGAATGCCCGGCTCCACAATGGATTGGCAAACCGCGTACGACTA
CCTGGTGCACGGCAACTACGATTCAGGAGAGCGAACTTTTTTTCAAGGCGTCCTGGCACTGCCGGCGGCCTACTGCTTTC
GCTATGAGCTGAACACGTTCACATGGAAGGCTTGGCGATGGTGGAAGCCAACGATCGCGCAAGGGTCGTGCAGGTCTGTT
GACGACGCAGCAGATGAGTTGCGCGCGTGTCTGCTGGACAGCGTGCGGTTGCATCTTCGCAGCGATGTCCCGCTGGGCGC
TGCGCTGTCGGGAGGGCTGGACTCTTCCGCCATCGTCGGTTGCATGCGGCACCTGGAGCCGGACGCTCCCATCCATACGT
TCAGTTTCGTCGCGCCCGGTAGCAGCACGTCGGAAGAGCGGTGGGTCGATCTGATGAACGACCACGTCGGGGCCCTGCAC
CACAAGGTGAGCATCGAGCCGGGGGAATTGGCCAGGGATCTTGAAGATTTGATCGCGACACAGGGTGAACCGTTCGGAAG
CACCAGCATTTATGCCCAATACCGAGTATTTCAGCTGGCCCGGGAACATGGCATGACCGTAACATTGGATGGGCAGGGGG
CCGACGAGTTGTGCGGGGGATACGCCGGTTATCCCGGTCCGCGGACGAGGAGTCTGTTGGACCAGGGCCGGTGGCTTGGT
GCCGCCCAGTTCCTGCGTCGCTGGGGCCGCTGGCCGGACCGTGTGGAAAGCGATGGATGGAAAGCCGCCGCTGCTGAATA
CATGAGCGATGCCGCCTACCAAGCAGCGCGGCGCATGAATGGGGAGAACGCCTGTCCCGAATGGCTGGATGGAAGCATTC
TTTCCGAGGCGGGTATCGTGATGCGCTTTCCGCGCCAGGTGCCGCACGTGACGGAGCGAGGCCGAAGGCTTCCCGCAGAG
CTTGCCCGGTCCCTGCACGGGACAGGACTGCAAGAGTTGCTACGCCATGGTGATCGCAACTCGATGCGTTTCTCCATAGA
AAGCCGCGTGCCTTTCCTGACCGTCAAGCTGGCGGACTTCCTTCTGGCGTTGCCTGAAGAGTACTTGGTCGCAGATGATG
GGGAAACCAAGCATGTGTTGCGGAAGGCCATGCGCGGACTGGTTCCGCGGGCAATACTGGACCGGCGCGACAAGATCGGA
TTTGCCACCCCCGAGCATGCTTGGCTGCTGAACATGGCCCCACAGATCCGTGAATGGTTGCGCGTGCCGCTGGGAGTGCC
CTTTCTACGCCAGCACGCGTTGCTGGAGGCGTTTGATTCCGTGGTGGCCGGCCGGCGTCCATACAGCTGGCAAGTATGGC
GCTGGATCAATTTCGCGCGATGGCACGCGAGGCACTTTCCTTCGTGA

Upstream 100 bases:

>100_bases
GTCTGCGGTCAATAAAATGAATATGAGAGATGACAAGCCTGCAAGGCGTGTTTCTTGAATGAATCACGGCTTCTGCTGTT
AAATGGAATAAAAATCGCTT

Downstream 100 bases:

>100_bases
CGATCCCATGGATCGCTTTTCCTCCACGTGGTTTCCTGCGCTGCTGCCGCTGCAGCAGCGACTGGCCGTGCCGGTGCCCG
GGGCTTCCGAGACTGTGCAG

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 628; Mature: 628

Protein sequence:

>628_residues
MCGIVGVFSSGDDPGSAGRPERFLKLLQHRGPDQQAYVRMQHGEGRGWLGHTRLKVIDLSDAAGQPMWSADGRYALLFNG
EIYNYRELRQELRIKGTHFRTDSDSEVLLHAWLAWGPDVLPRLVGMFAFVIYDREMATLHGARDAFGIKPLYYRCGGRDF
SFASESSVLSSMGMPGSTMDWQTAYDYLVHGNYDSGERTFFQGVLALPAAYCFRYELNTFTWKAWRWWKPTIAQGSCRSV
DDAADELRACLLDSVRLHLRSDVPLGAALSGGLDSSAIVGCMRHLEPDAPIHTFSFVAPGSSTSEERWVDLMNDHVGALH
HKVSIEPGELARDLEDLIATQGEPFGSTSIYAQYRVFQLAREHGMTVTLDGQGADELCGGYAGYPGPRTRSLLDQGRWLG
AAQFLRRWGRWPDRVESDGWKAAAAEYMSDAAYQAARRMNGENACPEWLDGSILSEAGIVMRFPRQVPHVTERGRRLPAE
LARSLHGTGLQELLRHGDRNSMRFSIESRVPFLTVKLADFLLALPEEYLVADDGETKHVLRKAMRGLVPRAILDRRDKIG
FATPEHAWLLNMAPQIREWLRVPLGVPFLRQHALLEAFDSVVAGRRPYSWQVWRWINFARWHARHFPS

Sequences:

>Translated_628_residues
MCGIVGVFSSGDDPGSAGRPERFLKLLQHRGPDQQAYVRMQHGEGRGWLGHTRLKVIDLSDAAGQPMWSADGRYALLFNG
EIYNYRELRQELRIKGTHFRTDSDSEVLLHAWLAWGPDVLPRLVGMFAFVIYDREMATLHGARDAFGIKPLYYRCGGRDF
SFASESSVLSSMGMPGSTMDWQTAYDYLVHGNYDSGERTFFQGVLALPAAYCFRYELNTFTWKAWRWWKPTIAQGSCRSV
DDAADELRACLLDSVRLHLRSDVPLGAALSGGLDSSAIVGCMRHLEPDAPIHTFSFVAPGSSTSEERWVDLMNDHVGALH
HKVSIEPGELARDLEDLIATQGEPFGSTSIYAQYRVFQLAREHGMTVTLDGQGADELCGGYAGYPGPRTRSLLDQGRWLG
AAQFLRRWGRWPDRVESDGWKAAAAEYMSDAAYQAARRMNGENACPEWLDGSILSEAGIVMRFPRQVPHVTERGRRLPAE
LARSLHGTGLQELLRHGDRNSMRFSIESRVPFLTVKLADFLLALPEEYLVADDGETKHVLRKAMRGLVPRAILDRRDKIG
FATPEHAWLLNMAPQIREWLRVPLGVPFLRQHALLEAFDSVVAGRRPYSWQVWRWINFARWHARHFPS
>Mature_628_residues
MCGIVGVFSSGDDPGSAGRPERFLKLLQHRGPDQQAYVRMQHGEGRGWLGHTRLKVIDLSDAAGQPMWSADGRYALLFNG
EIYNYRELRQELRIKGTHFRTDSDSEVLLHAWLAWGPDVLPRLVGMFAFVIYDREMATLHGARDAFGIKPLYYRCGGRDF
SFASESSVLSSMGMPGSTMDWQTAYDYLVHGNYDSGERTFFQGVLALPAAYCFRYELNTFTWKAWRWWKPTIAQGSCRSV
DDAADELRACLLDSVRLHLRSDVPLGAALSGGLDSSAIVGCMRHLEPDAPIHTFSFVAPGSSTSEERWVDLMNDHVGALH
HKVSIEPGELARDLEDLIATQGEPFGSTSIYAQYRVFQLAREHGMTVTLDGQGADELCGGYAGYPGPRTRSLLDQGRWLG
AAQFLRRWGRWPDRVESDGWKAAAAEYMSDAAYQAARRMNGENACPEWLDGSILSEAGIVMRFPRQVPHVTERGRRLPAE
LARSLHGTGLQELLRHGDRNSMRFSIESRVPFLTVKLADFLLALPEEYLVADDGETKHVLRKAMRGLVPRAILDRRDKIG
FATPEHAWLLNMAPQIREWLRVPLGVPFLRQHALLEAFDSVVAGRRPYSWQVWRWINFARWHARHFPS

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=419, Percent_Identity=30.0715990453461, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6321563, Length=416, Percent_Identity=26.4423076923077, Blast_Score=116, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6325403, Length=414, Percent_Identity=28.0193236714976, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI45553209, Length=408, Percent_Identity=30.6372549019608, Blast_Score=136, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70851; Mature: 70851

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGVFSSGDDPGSAGRPERFLKLLQHRGPDQQAYVRMQHGEGRGWLGHTRLKVIDLS
CCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCEEEEEECC
DAAGQPMWSADGRYALLFNGEIYNYRELRQELRIKGTHFRTDSDSEVLLHAWLAWGPDVL
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCEECCCCCCHHHHHHHHHCCHHHH
PRLVGMFAFVIYDREMATLHGARDAFGIKPLYYRCGGRDFSFASESSVLSSMGMPGSTMD
HHHHHHHHHHHHCCHHHHHHCCCHHCCCCCHHEECCCCCCCCCHHHHHHHHCCCCCCCCC
WQTAYDYLVHGNYDSGERTFFQGVLALPAAYCFRYELNTFTWKAWRWWKPTIAQGSCRSV
HHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCEEEEEEEEECCCCCCCCCCCH
DDAADELRACLLDSVRLHLRSDVPLGAALSGGLDSSAIVGCMRHLEPDAPIHTFSFVAPG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEECCC
SSTSEERWVDLMNDHVGALHHKVSIEPGELARDLEDLIATQGEPFGSTSIYAQYRVFQLA
CCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
REHGMTVTLDGQGADELCGGYAGYPGPRTRSLLDQGRWLGAAQFLRRWGRWPDRVESDGW
HHCCCEEEECCCCHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCCCCHHCCCCH
KAAAAEYMSDAAYQAARRMNGENACPEWLDGSILSEAGIVMRFPRQVPHVTERGRRLPAE
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHCCCEEECCHHCCCHHHHCCCCHHH
LARSLHGTGLQELLRHGDRNSMRFSIESRVPFLTVKLADFLLALPEEYLVADDGETKHVL
HHHHHCCCCHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHCCHHHEEECCCCHHHHH
RKAMRGLVPRAILDRRDKIGFATPEHAWLLNMAPQIREWLRVPLGVPFLRQHALLEAFDS
HHHHHHCCHHHHHCCCCCCCCCCCCCHHEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
VVAGRRPYSWQVWRWINFARWHARHFPS
HHHCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MCGIVGVFSSGDDPGSAGRPERFLKLLQHRGPDQQAYVRMQHGEGRGWLGHTRLKVIDLS
CCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCEEEEEECC
DAAGQPMWSADGRYALLFNGEIYNYRELRQELRIKGTHFRTDSDSEVLLHAWLAWGPDVL
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCEECCCCCCHHHHHHHHHCCHHHH
PRLVGMFAFVIYDREMATLHGARDAFGIKPLYYRCGGRDFSFASESSVLSSMGMPGSTMD
HHHHHHHHHHHHCCHHHHHHCCCHHCCCCCHHEECCCCCCCCCHHHHHHHHCCCCCCCCC
WQTAYDYLVHGNYDSGERTFFQGVLALPAAYCFRYELNTFTWKAWRWWKPTIAQGSCRSV
HHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCEEEEEEEEECCCCCCCCCCCH
DDAADELRACLLDSVRLHLRSDVPLGAALSGGLDSSAIVGCMRHLEPDAPIHTFSFVAPG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEECCC
SSTSEERWVDLMNDHVGALHHKVSIEPGELARDLEDLIATQGEPFGSTSIYAQYRVFQLA
CCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
REHGMTVTLDGQGADELCGGYAGYPGPRTRSLLDQGRWLGAAQFLRRWGRWPDRVESDGW
HHCCCEEEECCCCHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCCCCHHCCCCH
KAAAAEYMSDAAYQAARRMNGENACPEWLDGSILSEAGIVMRFPRQVPHVTERGRRLPAE
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHCCCEEECCHHCCCHHHHCCCCHHH
LARSLHGTGLQELLRHGDRNSMRFSIESRVPFLTVKLADFLLALPEEYLVADDGETKHVL
HHHHHCCCCHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHCCHHHEEECCCCHHHHH
RKAMRGLVPRAILDRRDKIGFATPEHAWLLNMAPQIREWLRVPLGVPFLRQHALLEAFDS
HHHHHHCCHHHHHCCCCCCCCCCCCCHHEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
VVAGRRPYSWQVWRWINFARWHARHFPS
HHHCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]