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The map label for this gene is cobS [H]

Identifier: 120609622

GI number: 120609622

Start: 1008938

End: 1009750

Strand: Reverse

Name: cobS [H]

Synonym: Aave_0928

Alternate gene names: 120609622

Gene position: 1009750-1008938 (Counterclockwise)

Preceding gene: 120609623

Following gene: 120609621

Centisome position: 18.86

GC content: 73.19

Gene sequence:

>813_bases
ATGCAGGCCCTTCGCCACTTCCTGCTGGCCGTGCAGTTCTTCACGCGCATCCCCGTGACCGGGCGGTGCGCGGACTGGGT
GGGGTTCAGCCCGGCCATGCTGCGGGCGAGCGCCGCGCATTTCCCCGGCGTGGGCTGGATCGTGGCGGCCGTGGCGTCCC
TGGTGTACGGCACGCTCCACCTGCTCTTCGGCCCCCAGCCCTATGCGGCATTGGCCTGTGCAGCGCTCAGCACCGCGGCG
ACCGTCGTGCTCACCGGAGGCTTTCACGAGGACGGCCTGGCGGACGTGGCGGACGGCCTGGGCGGCAGCGCGGACCGCGG
GCGGGCCCTGGAAATCATGAAGGATTCGCGCATTGGCGCGTTCGGGGCCATGGCCCTGTGCCTGGCCCTGGCCAGCAAGC
TCGCGATGCTGGCGATGCTGGGGCACCGCGGGCTGGCCGTGGCACTGGCGGCCCTGGCAGGCGCGCATGTGGTGTCCCGC
TTCTGGCCCCTGCTGCTGGTGCGCACATTGCCCCACGTGGGCCATGAGGCCACCTCCAAGAGCAAGCCGCTGGCGGACCG
GATCACGCTGGGCGCGCTGCTGGCAGCCGGCTTGTGGGCCGCGCCCGCGCTGGTGCTCGCAGCCTGGCTGCTGGGCCCGG
CATCGGCCCTGGCGGCCCTGGGCGCCAGCGGCGTGGCCGCCTGGACGCTGCGTGCATGGTTCGCCCGGCGCCTGCAGGGT
TTCACGGGGGACTGCCTGGGGGCGGTGCAGCAGGTGGCCGAGATCGCGTTCTACCTGGGATGCGCCGCGGTGGCCGGTAC
CGCTGCCACATGA

Upstream 100 bases:

>100_bases
AGAAACGCAGAACCCCGCGTACCGGCTGTTCCTGCAGGCGCCCGCGGGCCGGGGCTGACGCCTTCCGATCGCCAACGAGA
AGCGACCGCCCCGCTGCCCC

Downstream 100 bases:

>100_bases
GCGCGCGGCCCGTGCGGCTCTGGCTGGTGCGGCATGCCATGCCGTGCGTGGCCGCGGGTATCTGCTATGGATCGCTCGAT
GTGGCGCCGGACGCCCAGGC

Product: cobalamin-5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MQALRHFLLAVQFFTRIPVTGRCADWVGFSPAMLRASAAHFPGVGWIVAAVASLVYGTLHLLFGPQPYAALACAALSTAA
TVVLTGGFHEDGLADVADGLGGSADRGRALEIMKDSRIGAFGAMALCLALASKLAMLAMLGHRGLAVALAALAGAHVVSR
FWPLLLVRTLPHVGHEATSKSKPLADRITLGALLAAGLWAAPALVLAAWLLGPASALAALGASGVAAWTLRAWFARRLQG
FTGDCLGAVQQVAEIAFYLGCAAVAGTAAT

Sequences:

>Translated_270_residues
MQALRHFLLAVQFFTRIPVTGRCADWVGFSPAMLRASAAHFPGVGWIVAAVASLVYGTLHLLFGPQPYAALACAALSTAA
TVVLTGGFHEDGLADVADGLGGSADRGRALEIMKDSRIGAFGAMALCLALASKLAMLAMLGHRGLAVALAALAGAHVVSR
FWPLLLVRTLPHVGHEATSKSKPLADRITLGALLAAGLWAAPALVLAAWLLGPASALAALGASGVAAWTLRAWFARRLQG
FTGDCLGAVQQVAEIAFYLGCAAVAGTAAT
>Mature_270_residues
MQALRHFLLAVQFFTRIPVTGRCADWVGFSPAMLRASAAHFPGVGWIVAAVASLVYGTLHLLFGPQPYAALACAALSTAA
TVVLTGGFHEDGLADVADGLGGSADRGRALEIMKDSRIGAFGAMALCLALASKLAMLAMLGHRGLAVALAALAGAHVVSR
FWPLLLVRTLPHVGHEATSKSKPLADRITLGALLAAGLWAAPALVLAAWLLGPASALAALGASGVAAWTLRAWFARRLQG
FTGDCLGAVQQVAEIAFYLGCAAVAGTAAT

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=254, Percent_Identity=35.4330708661417, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 27618; Mature: 27618

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQALRHFLLAVQFFTRIPVTGRCADWVGFSPAMLRASAAHFPGVGWIVAAVASLVYGTLH
CHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LLFGPQPYAALACAALSTAATVVLTGGFHEDGLADVADGLGGSADRGRALEIMKDSRIGA
HHCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHCCCCCCCCCCHHHHHHHCCCCH
FGAMALCLALASKLAMLAMLGHRGLAVALAALAGAHVVSRFWPLLLVRTLPHVGHEATSK
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC
SKPLADRITLGALLAAGLWAAPALVLAAWLLGPASALAALGASGVAAWTLRAWFARRLQG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
FTGDCLGAVQQVAEIAFYLGCAAVAGTAAT
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQALRHFLLAVQFFTRIPVTGRCADWVGFSPAMLRASAAHFPGVGWIVAAVASLVYGTLH
CHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LLFGPQPYAALACAALSTAATVVLTGGFHEDGLADVADGLGGSADRGRALEIMKDSRIGA
HHCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHCCCCCCCCCCHHHHHHHCCCCH
FGAMALCLALASKLAMLAMLGHRGLAVALAALAGAHVVSRFWPLLLVRTLPHVGHEATSK
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC
SKPLADRITLGALLAAGLWAAPALVLAAWLLGPASALAALGASGVAAWTLRAWFARRLQG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
FTGDCLGAVQQVAEIAFYLGCAAVAGTAAT
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA