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The map label for this gene is phrA [H]

Identifier: 120609600

GI number: 120609600

Start: 988346

End: 989827

Strand: Reverse

Name: phrA [H]

Synonym: Aave_0906

Alternate gene names: 120609600

Gene position: 989827-988346 (Counterclockwise)

Preceding gene: 120609607

Following gene: 120609593

Centisome position: 18.49

GC content: 70.65

Gene sequence:

>1482_bases
ATGGAGCCCACTTTTTCCAAAGGCCTGGTCTGGTTCCGGCGCGACCTGCGCAGCGACGACCATGCCGCGCTCTACCACGC
ACTGCGCCAGTGCGGCCAGGTGCATTGCGTCTTCGTTTTCGACACGGCCATCCTGGATCCGCTGCCGCGGGCGGACCGGC
GCGTGGAGTTCATCCGCGAATCGCTGGTGCAGCTCGACGCGTCCCTGCGCGCCCTCTCGGGCCACCGCCACGGCGGGCTG
ATCGTGCGCCACGGCGTGGCCGCGGAGGAAGTGCCCGCGCTGGCCGCGCGACTGGGGGTGCAGGCCGTGTTCGCCAACCA
TGACGACGAGCCGGTCGCCCTGGAGCGCGACGACCGCGTCCGCCGCAGGCTGGGCACAGGCGGTGTGCAAATGCTCACTT
ACAAGGACCACGCGATTTTCGAACGCGCGGAGGTCCTGACCCAGGGCGGCACGCCCTACACCGTGTTCACGCCCTACAAG
AATGCCTGGCTGCGCAAGGTGGACGCGTTCTACCTGAGCGCCTATCCCGTGGAGCGCCATGCGGGCCGGCTCGCGCCCCG
TCCGGAAGGAGAGCGTGGCGGCGTACCCGCGCTGGCCGACATCGGCTTCGAGCCCGCGGGGCTGGACCGCCTGCCCCTGC
CGCCGGGCATGGACGGCGCGCGCGCGCTGCTGGACGATTTTCTCCAGCGGATCGACCAGTACGGCGAGGCACGCGACTTT
CCGGCCGTGAAGGGGCCGAGCTACCTCAGCGTGCACCTGCGCTTCGGCACGCTGTCGCCGCGGCGGGCGGCACGCGCCGC
GCACGAGCGCATGCGCCAGGGCAGCACCGGCGCCGCCACGTGGCTCAACGAGCTGATCTGGCGCGACTTCTACTTCCAGG
TGCTGGCCCACCATCCGCAGGTGGCCGGCGGCGCCAGCTTCAAGCCGGCCTACGACGCCATCGCCTGGGAGGACGGCCCG
CAGGCGCGGGAGCGTTTCGCCGCATGGTGCGAGGGCCGCACCGGCTACCCGCTGGTGGACGCCGCCATGGCCCAGATCAA
CCGGACGGGCTACATGCACAACCGGCTGCGCATGGTCGTGGCCAGTTTCCTGGTGAAGGACCTGGGCGTGGACTGGCGGT
GGGGGGAGCGCTATTTCGCGGAAAAGCTCAACGATTTCGACCTGTCCGCCAACAACGGCGGGTGGCAGTGGGCCAGTTCC
AGCGGCTGCGATGCGCAACCGTATTTCCGCATCTTCAACCCGGTGAGCCAGAGCCGGAAGTTCGATCCCAAGGGCCGCTT
CATCCGCCTCTACCTGCCCCAGCTGGCGGCCCTGCCCGACCGCTGGCTGCACGCCCCCTGGGAGGCCGGCCCACTGGAGC
TCGAGGCCGCCGGGGTGCGACTGGGCGACAACTACCCGCACCCCGTCGTGGACCACGACGAGGCGCGCCAGCGCACGCTG
GCGCGCTATGCCGTGGTGAAGTCGGAGGCCGCGCCGGCCTGA

Upstream 100 bases:

>100_bases
CAAACGACTCGTCTTCCAGCCCCGGCATGGCGATCAGGAAGTGGTGCGTCAGGTTCATGGGCGCAGAATCGGCAGGCATC
CCCCAATTTTAACGACGATC

Downstream 100 bases:

>100_bases
CGGACCGACCGACGGCCGGCCGGCTCCGCGTCAGCCGGCAGCGGCCTCCGCGGGCACGGCCTCCCCGCCGCCCTGCCCGG
CCGCAGCAGCGGCCGCGGCG

Product: deoxyribodipyrimidine photo-lyase type I

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MEPTFSKGLVWFRRDLRSDDHAALYHALRQCGQVHCVFVFDTAILDPLPRADRRVEFIRESLVQLDASLRALSGHRHGGL
IVRHGVAAEEVPALAARLGVQAVFANHDDEPVALERDDRVRRRLGTGGVQMLTYKDHAIFERAEVLTQGGTPYTVFTPYK
NAWLRKVDAFYLSAYPVERHAGRLAPRPEGERGGVPALADIGFEPAGLDRLPLPPGMDGARALLDDFLQRIDQYGEARDF
PAVKGPSYLSVHLRFGTLSPRRAARAAHERMRQGSTGAATWLNELIWRDFYFQVLAHHPQVAGGASFKPAYDAIAWEDGP
QARERFAAWCEGRTGYPLVDAAMAQINRTGYMHNRLRMVVASFLVKDLGVDWRWGERYFAEKLNDFDLSANNGGWQWASS
SGCDAQPYFRIFNPVSQSRKFDPKGRFIRLYLPQLAALPDRWLHAPWEAGPLELEAAGVRLGDNYPHPVVDHDEARQRTL
ARYAVVKSEAAPA

Sequences:

>Translated_493_residues
MEPTFSKGLVWFRRDLRSDDHAALYHALRQCGQVHCVFVFDTAILDPLPRADRRVEFIRESLVQLDASLRALSGHRHGGL
IVRHGVAAEEVPALAARLGVQAVFANHDDEPVALERDDRVRRRLGTGGVQMLTYKDHAIFERAEVLTQGGTPYTVFTPYK
NAWLRKVDAFYLSAYPVERHAGRLAPRPEGERGGVPALADIGFEPAGLDRLPLPPGMDGARALLDDFLQRIDQYGEARDF
PAVKGPSYLSVHLRFGTLSPRRAARAAHERMRQGSTGAATWLNELIWRDFYFQVLAHHPQVAGGASFKPAYDAIAWEDGP
QARERFAAWCEGRTGYPLVDAAMAQINRTGYMHNRLRMVVASFLVKDLGVDWRWGERYFAEKLNDFDLSANNGGWQWASS
SGCDAQPYFRIFNPVSQSRKFDPKGRFIRLYLPQLAALPDRWLHAPWEAGPLELEAAGVRLGDNYPHPVVDHDEARQRTL
ARYAVVKSEAAPA
>Mature_493_residues
MEPTFSKGLVWFRRDLRSDDHAALYHALRQCGQVHCVFVFDTAILDPLPRADRRVEFIRESLVQLDASLRALSGHRHGGL
IVRHGVAAEEVPALAARLGVQAVFANHDDEPVALERDDRVRRRLGTGGVQMLTYKDHAIFERAEVLTQGGTPYTVFTPYK
NAWLRKVDAFYLSAYPVERHAGRLAPRPEGERGGVPALADIGFEPAGLDRLPLPPGMDGARALLDDFLQRIDQYGEARDF
PAVKGPSYLSVHLRFGTLSPRRAARAAHERMRQGSTGAATWLNELIWRDFYFQVLAHHPQVAGGASFKPAYDAIAWEDGP
QARERFAAWCEGRTGYPLVDAAMAQINRTGYMHNRLRMVVASFLVKDLGVDWRWGERYFAEKLNDFDLSANNGGWQWASS
SGCDAQPYFRIFNPVSQSRKFDPKGRFIRLYLPQLAALPDRWLHAPWEAGPLELEAAGVRLGDNYPHPVVDHDEARQRTL
ARYAVVKSEAAPA

Specific function: Involved in repair of UV radiation-induced DNA damage. Exhibits specific, light-dependent repair activity for a cyclobutyl pyrimidine dimer by catalyzing the light-dependent monomerization (300-600 nm) of the dimers (in cis-syn configuration), which are f

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=490, Percent_Identity=31.2244897959184, Blast_Score=220, Evalue=3e-57,
Organism=Homo sapiens, GI4758072, Length=495, Percent_Identity=28.4848484848485, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI188536103, Length=454, Percent_Identity=30.8370044052863, Blast_Score=198, Evalue=8e-51,
Organism=Escherichia coli, GI1786926, Length=494, Percent_Identity=39.2712550607287, Blast_Score=306, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6324962, Length=510, Percent_Identity=29.8039215686275, Blast_Score=208, Evalue=2e-54,
Organism=Drosophila melanogaster, GI17137248, Length=509, Percent_Identity=29.0766208251473, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24585455, Length=509, Percent_Identity=29.0766208251473, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24648152, Length=514, Percent_Identity=27.6264591439689, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR019947
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 55370; Mature: 55370

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPTFSKGLVWFRRDLRSDDHAALYHALRQCGQVHCVFVFDTAILDPLPRADRRVEFIRE
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECHHHHCCCCCHHHHHHHHHH
SLVQLDASLRALSGHRHGGLIVRHGVAAEEVPALAARLGVQAVFANHDDEPVALERDDRV
HHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHEECCCCCCCEEECCHHHH
RRRLGTGGVQMLTYKDHAIFERAEVLTQGGTPYTVFTPYKNAWLRKVDAFYLSAYPVERH
HHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHH
AGRLAPRPEGERGGVPALADIGFEPAGLDRLPLPPGMDGARALLDDFLQRIDQYGEARDF
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
PAVKGPSYLSVHLRFGTLSPRRAARAAHERMRQGSTGAATWLNELIWRDFYFQVLAHHPQ
CCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCC
VAGGASFKPAYDAIAWEDGPQARERFAAWCEGRTGYPLVDAAMAQINRTGYMHNRLRMVV
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHH
ASFLVKDLGVDWRWGERYFAEKLNDFDLSANNGGWQWASSSGCDAQPYFRIFNPVSQSRK
HHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCHHHHHCHHHHCCC
FDPKGRFIRLYLPQLAALPDRWLHAPWEAGPLELEAAGVRLGDNYPHPVVDHDEARQRTL
CCCCCCEEEEECHHHHHCCHHHHCCCCCCCCEEEEECCEEECCCCCCCCCCCHHHHHHHH
ARYAVVKSEAAPA
HHHHHHHHCCCCC
>Mature Secondary Structure
MEPTFSKGLVWFRRDLRSDDHAALYHALRQCGQVHCVFVFDTAILDPLPRADRRVEFIRE
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECHHHHCCCCCHHHHHHHHHH
SLVQLDASLRALSGHRHGGLIVRHGVAAEEVPALAARLGVQAVFANHDDEPVALERDDRV
HHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHEECCCCCCCEEECCHHHH
RRRLGTGGVQMLTYKDHAIFERAEVLTQGGTPYTVFTPYKNAWLRKVDAFYLSAYPVERH
HHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHH
AGRLAPRPEGERGGVPALADIGFEPAGLDRLPLPPGMDGARALLDDFLQRIDQYGEARDF
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
PAVKGPSYLSVHLRFGTLSPRRAARAAHERMRQGSTGAATWLNELIWRDFYFQVLAHHPQ
CCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCC
VAGGASFKPAYDAIAWEDGPQARERFAAWCEGRTGYPLVDAAMAQINRTGYMHNRLRMVV
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHH
ASFLVKDLGVDWRWGERYFAEKLNDFDLSANNGGWQWASSSGCDAQPYFRIFNPVSQSRK
HHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCHHHHHCHHHHCCC
FDPKGRFIRLYLPQLAALPDRWLHAPWEAGPLELEAAGVRLGDNYPHPVVDHDEARQRTL
CCCCCCEEEEECHHHHHCCHHHHCCCCCCCCEEEEECCEEECCCCCCCCCCCHHHHHHHH
ARYAVVKSEAAPA
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11254140; 8905231; 10896222; 10871367 [H]