BacMap

Definition	Acidovorax citrulli AAC00-1 chromosome, complete genome.
Accession	NC_008752
Length	5,352,772

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The map label for this gene is fadB1 [H]

Identifier: 120609576

GI number: 120609576

Start: 958241

End: 959020

Strand: Reverse

Name: fadB1 [H]

Synonym: Aave_0882

Alternate gene names: 120609576

Gene position: 959020-958241 (Counterclockwise)

Preceding gene: 120609577

Following gene: 120609573

Centisome position: 17.92

GC content: 68.21

Gene sequence:

>780_bases
TTGGCCTACGAAGTCATCGAGGTCCGCACCGAGGCGGACAAGGTCGGCGTCATCACGCTGAACCGCCCCAAGCAGCTCAA
CGCGCTCAACGACCAGCTCATGGACGAGCTGGGCGACGCGCTGGCCGCCTTCGATGCCGACGAACGCATCGGCTGCATCA
TCCTCACCGGCAGTGAAAAGGCCTTCGCGGCGGGCGCGGACATCGGCGCGATGGCCAAGTACGGCTTCGCCGACGCCTAC
AAGGGCGACTACATCACGCGCAACTGGGAACGCATCCGCTCCATCCGCAAGCCGGTGATCGCGGCCGTGAGCGGCTTCGC
GCTGGGCGGCGGCTGCGAGCTGGCGATGATGTGCGACTTCATCATCGCCGCCGACAACGCGAAGTTCGGCCAGCCCGAGA
TCAAGCTCGGCGTGATTCCGGGCGCGGGCGGCACGCAGCGCCTGCCGCGCGCCGTGGGCAAGTCCAAGGCCATGGACATG
GCCCTCACCGCCCGCATGATGGATGCGCAGGAGGCCGAGCGTGCCGGCCTGGTGAGCCGCGTCGTGCCCTACGACAAGCT
CATGGACGAAGCCCTGGGCGCGGCCCTGGTGATCGCCGGCTTCTCGCAGGTGGCCGTCATGGCGGCCAAGGAAACCGTCA
ACCGCGCCTTCGAGGGGGCGCTCTCCGACGGCCTCATGTACGAGCGCCGCCTGTTCCACGCGCTGTTCGCCACGCAGGAC
CAGAAGGAAGGCATGGCCGCCTTCCTGGAAAAGCGCCCGGCGGTGTTCACGCACCGCTGA

Upstream 100 bases:

>100_bases
CGGACACGGGCACGGCCGCCGGGTAGCCTGCGGGACGGGCGCGAAAGGCGGCCCTTGGGTATAGTCGTCCGCACCACAAC
GACACCCAGGAGATACCCCC

Downstream 100 bases:

>100_bases
GCGCAGCGCGGCGAGAAAGGGGCTGAGGGCCGCGGCTCCGCGCCTGCCTGCGCAGGCTTGGACGTGTCCGAAGGGCTGCC
GACTCTGGCGGCTGCACGGA

Product: short chain enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MAYEVIEVRTEADKVGVITLNRPKQLNALNDQLMDELGDALAAFDADERIGCIILTGSEKAFAAGADIGAMAKYGFADAY
KGDYITRNWERIRSIRKPVIAAVSGFALGGGCELAMMCDFIIAADNAKFGQPEIKLGVIPGAGGTQRLPRAVGKSKAMDM
ALTARMMDAQEAERAGLVSRVVPYDKLMDEALGAALVIAGFSQVAVMAAKETVNRAFEGALSDGLMYERRLFHALFATQD
QKEGMAAFLEKRPAVFTHR

Sequences:

>Translated_259_residues
MAYEVIEVRTEADKVGVITLNRPKQLNALNDQLMDELGDALAAFDADERIGCIILTGSEKAFAAGADIGAMAKYGFADAY
KGDYITRNWERIRSIRKPVIAAVSGFALGGGCELAMMCDFIIAADNAKFGQPEIKLGVIPGAGGTQRLPRAVGKSKAMDM
ALTARMMDAQEAERAGLVSRVVPYDKLMDEALGAALVIAGFSQVAVMAAKETVNRAFEGALSDGLMYERRLFHALFATQD
QKEGMAAFLEKRPAVFTHR
>Mature_258_residues
AYEVIEVRTEADKVGVITLNRPKQLNALNDQLMDELGDALAAFDADERIGCIILTGSEKAFAAGADIGAMAKYGFADAYK
GDYITRNWERIRSIRKPVIAAVSGFALGGGCELAMMCDFIIAADNAKFGQPEIKLGVIPGAGGTQRLPRAVGKSKAMDMA
LTARMMDAQEAERAGLVSRVVPYDKLMDEALGAALVIAGFSQVAVMAAKETVNRAFEGALSDGLMYERRLFHALFATQDQ
KEGMAAFLEKRPAVFTHR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=54.3307086614173, Blast_Score=278, Evalue=3e-75,
Organism=Homo sapiens, GI4502327, Length=248, Percent_Identity=37.9032258064516, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI70995211, Length=265, Percent_Identity=32.8301886792453, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI68989263, Length=183, Percent_Identity=37.1584699453552, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI20127408, Length=224, Percent_Identity=33.9285714285714, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=30.2788844621514, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI37594469, Length=180, Percent_Identity=34.4444444444444, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI37594471, Length=180, Percent_Identity=34.4444444444444, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI213417737, Length=237, Percent_Identity=27.8481012658228, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI157694516, Length=237, Percent_Identity=27.8481012658228, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI150378541, Length=245, Percent_Identity=29.7959183673469, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI4757968, Length=262, Percent_Identity=22.9007633587786, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI49355787, Length=262, Percent_Identity=22.9007633587786, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI25453481, Length=256, Percent_Identity=23.4375, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI51538573, Length=256, Percent_Identity=23.4375, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI260274832, Length=247, Percent_Identity=27.5303643724696, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI45643119, Length=247, Percent_Identity=27.5303643724696, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI260275230, Length=247, Percent_Identity=27.5303643724696, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI4757966, Length=217, Percent_Identity=25.3456221198157, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI51538581, Length=217, Percent_Identity=25.3456221198157, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI221136756, Length=257, Percent_Identity=25.2918287937743, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI221136753, Length=257, Percent_Identity=25.2918287937743, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI221307494, Length=257, Percent_Identity=25.2918287937743, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI116235487, Length=254, Percent_Identity=24.8031496062992, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI261878539, Length=86, Percent_Identity=44.1860465116279, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI157694520, Length=200, Percent_Identity=28.5, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI62530384, Length=186, Percent_Identity=27.9569892473118, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=46.875, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI221142681, Length=247, Percent_Identity=38.8663967611336, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=35.6060606060606, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI1787660, Length=251, Percent_Identity=35.0597609561753, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87082183, Length=262, Percent_Identity=33.206106870229, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788682, Length=188, Percent_Identity=33.5106382978723, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790281, Length=183, Percent_Identity=30.0546448087432, Blast_Score=98, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=55.8704453441296, Blast_Score=274, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI25145438, Length=246, Percent_Identity=53.2520325203252, Blast_Score=255, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17560910, Length=253, Percent_Identity=33.201581027668, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17540306, Length=239, Percent_Identity=34.7280334728033, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17534483, Length=268, Percent_Identity=29.8507462686567, Blast_Score=116, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=31.8181818181818, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25144276, Length=224, Percent_Identity=33.9285714285714, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17508953, Length=224, Percent_Identity=33.9285714285714, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17508951, Length=224, Percent_Identity=33.9285714285714, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17536985, Length=274, Percent_Identity=29.5620437956204, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17558304, Length=213, Percent_Identity=30.9859154929577, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI25144157, Length=193, Percent_Identity=33.160621761658, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=193, Percent_Identity=33.160621761658, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=172, Percent_Identity=31.9767441860465, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=203, Percent_Identity=29.5566502463054, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=238, Percent_Identity=26.0504201680672, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=191, Percent_Identity=30.8900523560209, Blast_Score=95, Evalue=9e-21,
Organism=Drosophila melanogaster, GI20129971, Length=256, Percent_Identity=58.203125, Blast_Score=296, Evalue=7e-81,
Organism=Drosophila melanogaster, GI24653477, Length=256, Percent_Identity=58.203125, Blast_Score=296, Evalue=7e-81,
Organism=Drosophila melanogaster, GI24653139, Length=250, Percent_Identity=34.8, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21357171, Length=254, Percent_Identity=31.1023622047244, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24650670, Length=225, Percent_Identity=32.8888888888889, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19922422, Length=249, Percent_Identity=28.1124497991968, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19920382, Length=265, Percent_Identity=30.5660377358491, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI28571729, Length=184, Percent_Identity=30.9782608695652, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28571730, Length=184, Percent_Identity=30.9782608695652, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24583077, Length=174, Percent_Identity=33.9080459770115, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24583079, Length=174, Percent_Identity=33.9080459770115, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI19921000, Length=174, Percent_Identity=33.9080459770115, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24654903, Length=171, Percent_Identity=29.8245614035088, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19921018, Length=218, Percent_Identity=29.8165137614679, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583165, Length=174, Percent_Identity=28.1609195402299, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27906; Mature: 27774

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYEVIEVRTEADKVGVITLNRPKQLNALNDQLMDELGDALAAFDADERIGCIILTGSEK
CCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
AFAAGADIGAMAKYGFADAYKGDYITRNWERIRSIRKPVIAAVSGFALGGGCELAMMCDF
CEECCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHH
IIAADNAKFGQPEIKLGVIPGAGGTQRLPRAVGKSKAMDMALTARMMDAQEAERAGLVSR
HEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVPYDKLMDEALGAALVIAGFSQVAVMAAKETVNRAFEGALSDGLMYERRLFHALFATQD
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
QKEGMAAFLEKRPAVFTHR
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AYEVIEVRTEADKVGVITLNRPKQLNALNDQLMDELGDALAAFDADERIGCIILTGSEK
CCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
AFAAGADIGAMAKYGFADAYKGDYITRNWERIRSIRKPVIAAVSGFALGGGCELAMMCDF
CEECCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHH
IIAADNAKFGQPEIKLGVIPGAGGTQRLPRAVGKSKAMDMALTARMMDAQEAERAGLVSR
HEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVPYDKLMDEALGAALVIAGFSQVAVMAAKETVNRAFEGALSDGLMYERRLFHALFATQD
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
QKEGMAAFLEKRPAVFTHR
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]