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The map label for this gene is murF [H]

Identifier: 120609511

GI number: 120609511

Start: 883341

End: 884846

Strand: Direct

Name: murF [H]

Synonym: Aave_0817

Alternate gene names: 120609511

Gene position: 883341-884846 (Clockwise)

Preceding gene: 120609510

Following gene: 120609512

Centisome position: 16.5

GC content: 73.37

Gene sequence:

>1506_bases
ATGAGCGGCGCTCCCATGCTCACGCTGCAGCAGGCCCATGCCCTCGTGCAGGCCCGCATCCCGCGGGCGCGCCTCGTGGG
CGAGCCAGGCACGCCCCTTCTGCGCGTGCACACCGACACGCGCACGCTGCAGGCGGGCGACCTGTTCGTGGCGCTGCGCG
GCGAACGCTTCGATGCCAACGATTTCCTCGCGCAGGCGCGTGCGGGCGGCGCGGCAGCGGCCCTGGCCCACCCGGGCCGG
CTCGATGCCGCGGGACTGCCCGGCATCGAGGTGCCCGACACCCTGGCCGCCCTGGGCGCGCTGGCGGCGGGCTGGCGCGC
GCGCCTCGACCTGCCCCTGGTCGGCGTCACGGGCAGCAACGGCAAGACCACCGTCACCCAGATGATCGCGTCGGTGCTGC
GCGCCTGGAAGGGCGACGCCGCATTTGCAACGCAAGGCAACTTCAACAACGACATCGGCGTGCCGCTCATGCTGCTGCGC
CTGCGCGACGCGCACGAGGCCGCGGTGATCGAGCTCGGCATGAACCACCCGGGCGAGATCGCGGTGCTGGCCGGCATCGC
TCGGCCCACGGTCGCGCTGGTCAACAACGCCCAGCGCGAGCACCTCGAGTTCATGCACACGGTGCAGGCGGTGGCGGAGG
AGAACGGTTCGGTGATCTCCGCGCTGCCTCCCGATGGCGTGGCCGTGTTCCCGGCCGCGGACACCTATGCGCCGCTCTGG
CGCGGACTGGCCGGCACCCGCCGGTGCCTCACGTTCTGCGACCGGGCCGGCGTGGCGGCGGACGTGCGCTGCACGGATGC
GCGCTGGGAGACCGGCGCCTGGAACGTACAGATCGACACGCCGCAAGGCGCGTTCGATGGCACTCTGCGCATCGCGGGGC
GCCACAACGTGGCCAATGCGCTGGCCGCCACGGCCTGCGCGCTGGCCGCGGGTGCCCCGCTGGACGCCATCGCGCGCGGC
CTCGCGGCTTTCGAACCCGTCAAGGGGCGTTCGCGCGCCTTCGTCGCCACCGTGGCGCAGCCGGGCGGAGCGCCGCGGCG
GATCACGGCGGTGGACGACACGTACAACGCCAACCCCGATTCCATGCGCGCGGCCATCGACGTGCTGGCCGAACTGCCCG
GCCCGCAATTGCTGGTGATGGGCGACATGGGGGAAGTGGGCGACCAGGGCCCGCAGTTCCACGCGGAGGCGGGCGGGCAC
GCTCGCGAGCGCGGCATCGGCCGCCTGTTCGCGCTGGGAGCGCTCGGCATTCACGCCGTGCAGGCCTTCGGGCCGCAGGC
ACGGCATTTCGACGACATGGCATCGCTGCTCGCGGCGGTGCGCAAGGCCGCGCCCGAGTCGGGCAGCGTGCTGGTGAAGG
GATCGCGGTTCATGAAGATGGAACAGGTGGTGCAGGCGCTCGAAGCGCCGGACGTGTTGGCGCAGGAACGCCATCGGCCG
GACGGCCCGGGCGTGCCGGGCGCGGCGCGCGCCGCCATCGCCGGGAGGGACACCGCATGCTCCTGA

Upstream 100 bases:

>100_bases
AGACCGCGGGCGTGCGCCGGCCTTTCTCGGACGCCGCCCATGCGCGCGCCGCGCTGGCGCGGCGCGAGTCCGCGGCCGGC
GGGGATGCGGGGGGCACGGC

Downstream 100 bases:

>100_bases
TGCTCTCCCAGTGGCTGCAGGGCCTGTCGCCCGAATTCGGCTTCTTCCGTGTCTTCCAGTACCTGACCTTCCGCGCGGTG
ATGGCGGCCATGACGGCGCT

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MSGAPMLTLQQAHALVQARIPRARLVGEPGTPLLRVHTDTRTLQAGDLFVALRGERFDANDFLAQARAGGAAAALAHPGR
LDAAGLPGIEVPDTLAALGALAAGWRARLDLPLVGVTGSNGKTTVTQMIASVLRAWKGDAAFATQGNFNNDIGVPLMLLR
LRDAHEAAVIELGMNHPGEIAVLAGIARPTVALVNNAQREHLEFMHTVQAVAEENGSVISALPPDGVAVFPAADTYAPLW
RGLAGTRRCLTFCDRAGVAADVRCTDARWETGAWNVQIDTPQGAFDGTLRIAGRHNVANALAATACALAAGAPLDAIARG
LAAFEPVKGRSRAFVATVAQPGGAPRRITAVDDTYNANPDSMRAAIDVLAELPGPQLLVMGDMGEVGDQGPQFHAEAGGH
ARERGIGRLFALGALGIHAVQAFGPQARHFDDMASLLAAVRKAAPESGSVLVKGSRFMKMEQVVQALEAPDVLAQERHRP
DGPGVPGAARAAIAGRDTACS

Sequences:

>Translated_501_residues
MSGAPMLTLQQAHALVQARIPRARLVGEPGTPLLRVHTDTRTLQAGDLFVALRGERFDANDFLAQARAGGAAAALAHPGR
LDAAGLPGIEVPDTLAALGALAAGWRARLDLPLVGVTGSNGKTTVTQMIASVLRAWKGDAAFATQGNFNNDIGVPLMLLR
LRDAHEAAVIELGMNHPGEIAVLAGIARPTVALVNNAQREHLEFMHTVQAVAEENGSVISALPPDGVAVFPAADTYAPLW
RGLAGTRRCLTFCDRAGVAADVRCTDARWETGAWNVQIDTPQGAFDGTLRIAGRHNVANALAATACALAAGAPLDAIARG
LAAFEPVKGRSRAFVATVAQPGGAPRRITAVDDTYNANPDSMRAAIDVLAELPGPQLLVMGDMGEVGDQGPQFHAEAGGH
ARERGIGRLFALGALGIHAVQAFGPQARHFDDMASLLAAVRKAAPESGSVLVKGSRFMKMEQVVQALEAPDVLAQERHRP
DGPGVPGAARAAIAGRDTACS
>Mature_500_residues
SGAPMLTLQQAHALVQARIPRARLVGEPGTPLLRVHTDTRTLQAGDLFVALRGERFDANDFLAQARAGGAAAALAHPGRL
DAAGLPGIEVPDTLAALGALAAGWRARLDLPLVGVTGSNGKTTVTQMIASVLRAWKGDAAFATQGNFNNDIGVPLMLLRL
RDAHEAAVIELGMNHPGEIAVLAGIARPTVALVNNAQREHLEFMHTVQAVAEENGSVISALPPDGVAVFPAADTYAPLWR
GLAGTRRCLTFCDRAGVAADVRCTDARWETGAWNVQIDTPQGAFDGTLRIAGRHNVANALAATACALAAGAPLDAIARGL
AAFEPVKGRSRAFVATVAQPGGAPRRITAVDDTYNANPDSMRAAIDVLAELPGPQLLVMGDMGEVGDQGPQFHAEAGGHA
RERGIGRLFALGALGIHAVQAFGPQARHFDDMASLLAAVRKAAPESGSVLVKGSRFMKMEQVVQALEAPDVLAQERHRPD
GPGVPGAARAAIAGRDTACS

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=436, Percent_Identity=42.2018348623853, Blast_Score=267, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 52056; Mature: 51925

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAPMLTLQQAHALVQARIPRARLVGEPGTPLLRVHTDTRTLQAGDLFVALRGERFDAN
CCCCCEEHHHHHHHHHHHCCCHHHCCCCCCCCEEEEECCCCEEECCCEEEEEECCCCCHH
DFLAQARAGGAAAALAHPGRLDAAGLPGIEVPDTLAALGALAAGWRARLDLPLVGVTGSN
HHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCCEEEEEECCC
GKTTVTQMIASVLRAWKGDAAFATQGNFNNDIGVPLMLLRLRDAHEAAVIELGMNHPGEI
CCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHEEHHHCCCCEEEEEECCCCCCCE
AVLAGIARPTVALVNNAQREHLEFMHTVQAVAEENGSVISALPPDGVAVFPAADTYAPLW
EEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCHHHHH
RGLAGTRRCLTFCDRAGVAADVRCTDARWETGAWNVQIDTPQGAFDGTLRIAGRHNVANA
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCEEEEECCCCHHHH
LAATACALAAGAPLDAIARGLAAFEPVKGRSRAFVATVAQPGGAPRRITAVDDTYNANPD
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCHH
SMRAAIDVLAELPGPQLLVMGDMGEVGDQGPQFHAEAGGHARERGIGRLFALGALGIHAV
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEECCCCCCHHHHCHHHHHHHHHHHHHHH
QAFGPQARHFDDMASLLAAVRKAAPESGSVLVKGSRFMKMEQVVQALEAPDVLAQERHRP
HHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHCCCHHHHHHCCCC
DGPGVPGAARAAIAGRDTACS
CCCCCCCHHHHEECCCCCCCC
>Mature Secondary Structure 
SGAPMLTLQQAHALVQARIPRARLVGEPGTPLLRVHTDTRTLQAGDLFVALRGERFDAN
CCCCEEHHHHHHHHHHHCCCHHHCCCCCCCCEEEEECCCCEEECCCEEEEEECCCCCHH
DFLAQARAGGAAAALAHPGRLDAAGLPGIEVPDTLAALGALAAGWRARLDLPLVGVTGSN
HHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCCEEEEEECCC
GKTTVTQMIASVLRAWKGDAAFATQGNFNNDIGVPLMLLRLRDAHEAAVIELGMNHPGEI
CCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHEEHHHCCCCEEEEEECCCCCCCE
AVLAGIARPTVALVNNAQREHLEFMHTVQAVAEENGSVISALPPDGVAVFPAADTYAPLW
EEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCHHHHH
RGLAGTRRCLTFCDRAGVAADVRCTDARWETGAWNVQIDTPQGAFDGTLRIAGRHNVANA
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCEEEEECCCCHHHH
LAATACALAAGAPLDAIARGLAAFEPVKGRSRAFVATVAQPGGAPRRITAVDDTYNANPD
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCHH
SMRAAIDVLAELPGPQLLVMGDMGEVGDQGPQFHAEAGGHARERGIGRLFALGALGIHAV
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEECCCCCCHHHHCHHHHHHHHHHHHHHH
QAFGPQARHFDDMASLLAAVRKAAPESGSVLVKGSRFMKMEQVVQALEAPDVLAQERHRP
HHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHCCCHHHHHHCCCC
DGPGVPGAARAAIAGRDTACS
CCCCCCCHHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]