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The map label for this gene is murE [H]

Identifier: 120609510

GI number: 120609510

Start: 881785

End: 883344

Strand: Direct

Name: murE [H]

Synonym: Aave_0816

Alternate gene names: 120609510

Gene position: 881785-883344 (Clockwise)

Preceding gene: 120609509

Following gene: 120609511

Centisome position: 16.47

GC content: 74.49

Gene sequence:

>1560_bases
ATGACCGAGCCCCAATCCCCATCAGGGCATCCCACGCCCCCGCTGCTCGGCTCCGTGCCGGAGGCGCTGGAGTGGCTGCG
CGCCCATGCCCCGCAGGGTGCGCTGCGCACGGACAGCCGCCAGGTCGAGCCTGGCGACATCTTCATCGCCTGGCCCGGCG
CTGCCACCGATGGCCGCATGCACGTGGGCGAGGCCCTCGCGCGCGGCGCGGCGGCCTGCCTGGTGGAGCGCGAAGGCGTG
GAGGCCTTCCGCTTCGGCGCCGAAGGCGTGGCGGCGCTGCGCGACCTCAAGGCGGCCACCGGCCTGCTGGCCGCCGACTG
GTTCGGCATGCCCACGCAGCGCCTGGACGTGGTGGCGGTGACCGGCACCAACGGCAAGACCAGCACCACCTGGTGGATCG
CCGATGCGCTCAACCTGCTCGCAGGCAGCGGGCTGGCCACCCATGGCGGCTGCGGCCTGATCGGCACCCTGGGCGTGGGC
GTGCCGCCCTCGCTGCAGGGCACGGGCCTGACCACGCCCGATCCCGTGCGGCTGCAGCAGGCCTTCGCCGGTTTCGTGGC
CAACGGGCTGGGCGCCTGCGCCATCGAGGCGTCCTCCATCGGGCTCGCGGAGCACCGCCTCGCGGGCATGCGCCTGCGCG
TGGCCGTATTCACCAACTTCACGCAGGACCACCTGGACTACCACGCGGGCATGGCGGCCTACTGGCAGGCCAAGAGCGCG
CTCTTCGACTGGCCAGGGCTGCAGGCGGCGGTCGTGAACATCGACGATCCGCGCGGCGGTGAACTGTCGGACACGCTCGC
GCAGCGCCCGATCGACCTCTGGACCACCTCGATCACCGGCCCGGCCCGGCTGCAGGCCCGCGACGTCACGTGGGGCGAGG
GCGGCCTGCGCTTCACGGTGGCCGAAGGCGTGCAGGCCCACGTGCTGCAGACGCGCGTGATCGGCCACTACAACATCCAC
AACCTGCTCGCCGTGGTCGCCAGCCTGCGCGCGCTGGGCATTCCGCTGGAGCACGCGCTCTGGGCCTGCGCGCAGCTCGC
GCCCGTGCCCGGCCGCATGGAGCTCATCCACGAGCCGGGCCAGCCGCTGGTGGCCGTGGACTACGCCCACACGCCCGATG
CGCTCGAGCAGGCCCTGCAGGCGCTGCGCCCCGTGGCGGAGCAGCGCGGCGGGCGGCTGTGGTGCGTTTTCGGCTGCGGC
GGCAACCGCGACCGGGCCAAGCGGCCGCTCATGGGCCGCATCGCGCAGCAGCGCGCCGACGCGGTGATCGTGACCAGCGA
CAACCCACGCAACGAGGCGCCCGGCGCCATCCTGCACGAGATCCTGCTGGGCACGGTGGCCGGCACCACGGTGCAGGCCG
AGCCCGAGCGCGATGCCGCCATCGCGCGCGCCATCGCCGACGCGGACGGGCGCGACGTGGTGCTCATCGCGGGCAAGGGG
CACGAGGACTACCAGGAGACCGCGGGCGTGCGCCGGCCTTTCTCGGACGCCGCCCATGCGCGCGCCGCGCTGGCGCGGCG
CGAGTCCGCGGCCGGCGGGGATGCGGGGGGCACGGCATGA

Upstream 100 bases:

>100_bases
TGTTCAGTGAGGTCGTGCAGCAGACGCTGCGCATGATGGGCGTGCCGCCGGACATGGCCGTCAAGCCCCGGATCGTCTCC
AACGGCGTGGAGGAGTCGCT

Downstream 100 bases:

>100_bases
GCGGCGCTCCCATGCTCACGCTGCAGCAGGCCCATGCCCTCGTGCAGGCCCGCATCCCGCGGGCGCGCCTCGTGGGCGAG
CCAGGCACGCCCCTTCTGCG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MTEPQSPSGHPTPPLLGSVPEALEWLRAHAPQGALRTDSRQVEPGDIFIAWPGAATDGRMHVGEALARGAAACLVEREGV
EAFRFGAEGVAALRDLKAATGLLAADWFGMPTQRLDVVAVTGTNGKTSTTWWIADALNLLAGSGLATHGGCGLIGTLGVG
VPPSLQGTGLTTPDPVRLQQAFAGFVANGLGACAIEASSIGLAEHRLAGMRLRVAVFTNFTQDHLDYHAGMAAYWQAKSA
LFDWPGLQAAVVNIDDPRGGELSDTLAQRPIDLWTTSITGPARLQARDVTWGEGGLRFTVAEGVQAHVLQTRVIGHYNIH
NLLAVVASLRALGIPLEHALWACAQLAPVPGRMELIHEPGQPLVAVDYAHTPDALEQALQALRPVAEQRGGRLWCVFGCG
GNRDRAKRPLMGRIAQQRADAVIVTSDNPRNEAPGAILHEILLGTVAGTTVQAEPERDAAIARAIADADGRDVVLIAGKG
HEDYQETAGVRRPFSDAAHARAALARRESAAGGDAGGTA

Sequences:

>Translated_519_residues
MTEPQSPSGHPTPPLLGSVPEALEWLRAHAPQGALRTDSRQVEPGDIFIAWPGAATDGRMHVGEALARGAAACLVEREGV
EAFRFGAEGVAALRDLKAATGLLAADWFGMPTQRLDVVAVTGTNGKTSTTWWIADALNLLAGSGLATHGGCGLIGTLGVG
VPPSLQGTGLTTPDPVRLQQAFAGFVANGLGACAIEASSIGLAEHRLAGMRLRVAVFTNFTQDHLDYHAGMAAYWQAKSA
LFDWPGLQAAVVNIDDPRGGELSDTLAQRPIDLWTTSITGPARLQARDVTWGEGGLRFTVAEGVQAHVLQTRVIGHYNIH
NLLAVVASLRALGIPLEHALWACAQLAPVPGRMELIHEPGQPLVAVDYAHTPDALEQALQALRPVAEQRGGRLWCVFGCG
GNRDRAKRPLMGRIAQQRADAVIVTSDNPRNEAPGAILHEILLGTVAGTTVQAEPERDAAIARAIADADGRDVVLIAGKG
HEDYQETAGVRRPFSDAAHARAALARRESAAGGDAGGTA
>Mature_518_residues
TEPQSPSGHPTPPLLGSVPEALEWLRAHAPQGALRTDSRQVEPGDIFIAWPGAATDGRMHVGEALARGAAACLVEREGVE
AFRFGAEGVAALRDLKAATGLLAADWFGMPTQRLDVVAVTGTNGKTSTTWWIADALNLLAGSGLATHGGCGLIGTLGVGV
PPSLQGTGLTTPDPVRLQQAFAGFVANGLGACAIEASSIGLAEHRLAGMRLRVAVFTNFTQDHLDYHAGMAAYWQAKSAL
FDWPGLQAAVVNIDDPRGGELSDTLAQRPIDLWTTSITGPARLQARDVTWGEGGLRFTVAEGVQAHVLQTRVIGHYNIHN
LLAVVASLRALGIPLEHALWACAQLAPVPGRMELIHEPGQPLVAVDYAHTPDALEQALQALRPVAEQRGGRLWCVFGCGG
NRDRAKRPLMGRIAQQRADAVIVTSDNPRNEAPGAILHEILLGTVAGTTVQAEPERDAAIARAIADADGRDVVLIAGKGH
EDYQETAGVRRPFSDAAHARAALARRESAAGGDAGGTA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=480, Percent_Identity=40.625, Blast_Score=271, Evalue=6e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 54460; Mature: 54329

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPQSPSGHPTPPLLGSVPEALEWLRAHAPQGALRTDSRQVEPGDIFIAWPGAATDGRM
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCH
HVGEALARGAAACLVEREGVEAFRFGAEGVAALRDLKAATGLLAADWFGMPTQRLDVVAV
HHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
TGTNGKTSTTWWIADALNLLAGSGLATHGGCGLIGTLGVGVPPSLQGTGLTTPDPVRLQQ
ECCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHH
AFAGFVANGLGACAIEASSIGLAEHRLAGMRLRVAVFTNFTQDHLDYHAGMAAYWQAKSA
HHHHHHHCCCCEEEEECCCCCHHHHHHCCEEEEEEEEECCCHHHHHHHCCHHHHHHHHHH
LFDWPGLQAAVVNIDDPRGGELSDTLAQRPIDLWTTSITGPARLQARDVTWGEGGLRFTV
HCCCCCCEEEEEECCCCCCCCHHHHHHHCCHHHEEECCCCCCEEEEEECEECCCCEEEEE
AEGVQAHVLQTRVIGHYNIHNLLAVVASLRALGIPLEHALWACAQLAPVPGRMELIHEPG
ECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHEECCC
QPLVAVDYAHTPDALEQALQALRPVAEQRGGRLWCVFGCGGNRDRAKRPLMGRIAQQRAD
CCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCHHHHHHHHHCC
AVIVTSDNPRNEAPGAILHEILLGTVAGTTVQAEPERDAAIARAIADADGRDVVLIAGKG
EEEEECCCCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCCEEEEEECCC
HEDYQETAGVRRPFSDAAHARAALARRESAAGGDAGGTA
CCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TEPQSPSGHPTPPLLGSVPEALEWLRAHAPQGALRTDSRQVEPGDIFIAWPGAATDGRM
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCH
HVGEALARGAAACLVEREGVEAFRFGAEGVAALRDLKAATGLLAADWFGMPTQRLDVVAV
HHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
TGTNGKTSTTWWIADALNLLAGSGLATHGGCGLIGTLGVGVPPSLQGTGLTTPDPVRLQQ
ECCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHH
AFAGFVANGLGACAIEASSIGLAEHRLAGMRLRVAVFTNFTQDHLDYHAGMAAYWQAKSA
HHHHHHHCCCCEEEEECCCCCHHHHHHCCEEEEEEEEECCCHHHHHHHCCHHHHHHHHHH
LFDWPGLQAAVVNIDDPRGGELSDTLAQRPIDLWTTSITGPARLQARDVTWGEGGLRFTV
HCCCCCCEEEEEECCCCCCCCHHHHHHHCCHHHEEECCCCCCEEEEEECEECCCCEEEEE
AEGVQAHVLQTRVIGHYNIHNLLAVVASLRALGIPLEHALWACAQLAPVPGRMELIHEPG
ECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHEECCC
QPLVAVDYAHTPDALEQALQALRPVAEQRGGRLWCVFGCGGNRDRAKRPLMGRIAQQRAD
CCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCHHHHHHHHHCC
AVIVTSDNPRNEAPGAILHEILLGTVAGTTVQAEPERDAAIARAIADADGRDVVLIAGKG
EEEEECCCCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCCEEEEEECCC
HEDYQETAGVRRPFSDAAHARAALARRESAAGGDAGGTA
CCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA