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The map label for this gene is yomI [H]

Identifier: 120609179

GI number: 120609179

Start: 512546

End: 513316

Strand: Direct

Name: yomI [H]

Synonym: Aave_0476

Alternate gene names: 120609179

Gene position: 512546-513316 (Clockwise)

Preceding gene: 120609178

Following gene: 120609184

Centisome position: 9.58

GC content: 75.23

Gene sequence:

>771_bases
ATGGCGCGGCCTGCTTCCCGGATGCGGGCCGCCCGCCGCATGCTGGCCGGCGTGTTCGCCCTGTCGGCCGGCGCGGCCAT
GGCGGCGGGTGCCTACGACTGCCGGCTGCCCGGCGGGGCGGCCCTGCTCGTGGTGGGCGCGGACATCGCGCAGCGCTTTC
CTTCCATCGGCAGTTCGTGCAACGCGGCCGCCTGGACGCCCGCGCGCGACGTTGCGCTGCCCGGCTGGCCGGACACCTCG
GCGATCGCCGCCGCGCGTGTGATCCACGCGCCGGCGCCCCGCGACATCTACGCACCCACGGCCGGCGCCGCGCTGCCGGG
CATGCGCTGGCCGGAAGGCGGCGCGCGGCGCGGGCCCGGGATCAGCCCCCGGGCGGAAGCGCTGTCGCCCATCATCGAAT
CCGCGGCGCAGCGCCACGCCATCGATCCCCACCTGGTGCGCGCGGTCATCCAGGTCGAGTCCGGCTATGCCCCCCGCGCG
CGGTCGCCCAAGGGCGCGATGGGCCTGATGCAGCTCATGCCCGCCACGGCCGCACGGTTCGGGGCCGCCTCCGAGGACGA
CATCCTCAGCCCCGCGGTGAATGTCGATGTGGGCGTGCGCTACCTCCGGTTCCTGGCCGACCGCTTCGGCGGCCGCACCG
ACCTCGTGCTGGCCGCCTACAACGCGGGCGAGGGCGCCGTCATCCGCCACGGCTACCGCGTGCCGCCCTACCGCGAGACC
CAGGACTACGTGCGCAAGGTCCTGGATCTGTATCCGCTGGCGAAACGCTAG

Upstream 100 bases:

>100_bases
TGGGCCGCGACGGCGTGCTGGATGCCGCCGAACTCGCGGAAAGCGCCCGGCGATACCTCGCGCAGCGTGCTCCGCTGCTG
CGCCGGCTGGAGGTGGTGTG

Downstream 100 bases:

>100_bases
GCAAAACAAAAGGCGGACCGACCGGTCCGCCTTCATGCGCCGCCGCCGCAAGGGGGGCTTGTTCGCCGCGCCTACTGCAG
GAAGCCCTGGAAGCCCGCGA

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MARPASRMRAARRMLAGVFALSAGAAMAAGAYDCRLPGGAALLVVGADIAQRFPSIGSSCNAAAWTPARDVALPGWPDTS
AIAAARVIHAPAPRDIYAPTAGAALPGMRWPEGGARRGPGISPRAEALSPIIESAAQRHAIDPHLVRAVIQVESGYAPRA
RSPKGAMGLMQLMPATAARFGAASEDDILSPAVNVDVGVRYLRFLADRFGGRTDLVLAAYNAGEGAVIRHGYRVPPYRET
QDYVRKVLDLYPLAKR

Sequences:

>Translated_256_residues
MARPASRMRAARRMLAGVFALSAGAAMAAGAYDCRLPGGAALLVVGADIAQRFPSIGSSCNAAAWTPARDVALPGWPDTS
AIAAARVIHAPAPRDIYAPTAGAALPGMRWPEGGARRGPGISPRAEALSPIIESAAQRHAIDPHLVRAVIQVESGYAPRA
RSPKGAMGLMQLMPATAARFGAASEDDILSPAVNVDVGVRYLRFLADRFGGRTDLVLAAYNAGEGAVIRHGYRVPPYRET
QDYVRKVLDLYPLAKR
>Mature_255_residues
ARPASRMRAARRMLAGVFALSAGAAMAAGAYDCRLPGGAALLVVGADIAQRFPSIGSSCNAAAWTPARDVALPGWPDTSA
IAAARVIHAPAPRDIYAPTAGAALPGMRWPEGGARRGPGISPRAEALSPIIESAAQRHAIDPHLVRAVIQVESGYAPRAR
SPKGAMGLMQLMPATAARFGAASEDDILSPAVNVDVGVRYLRFLADRFGGRTDLVLAAYNAGEGAVIRHGYRVPPYRETQ
DYVRKVLDLYPLAKR

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 10 TPR repeats [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=120, Percent_Identity=35, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI87082191, Length=124, Percent_Identity=31.4516129032258, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR008258
- InterPro:   IPR016047
- InterPro:   IPR010090
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01551 Peptidase_M23; PF10145 PhageMin_Tail; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 26931; Mature: 26800

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPASRMRAARRMLAGVFALSAGAAMAAGAYDCRLPGGAALLVVGADIAQRFPSIGSSC
CCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEECCCCCEEEEECCHHHHHHHHCCCCC
NAAAWTPARDVALPGWPDTSAIAAARVIHAPAPRDIYAPTAGAALPGMRWPEGGARRGPG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ISPRAEALSPIIESAAQRHAIDPHLVRAVIQVESGYAPRARSPKGAMGLMQLMPATAARF
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
GAASEDDILSPAVNVDVGVRYLRFLADRFGGRTDLVLAAYNAGEGAVIRHGYRVPPYRET
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCCCCCCCCCH
QDYVRKVLDLYPLAKR
HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ARPASRMRAARRMLAGVFALSAGAAMAAGAYDCRLPGGAALLVVGADIAQRFPSIGSSC
CCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEECCCCCEEEEECCHHHHHHHHCCCCC
NAAAWTPARDVALPGWPDTSAIAAARVIHAPAPRDIYAPTAGAALPGMRWPEGGARRGPG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ISPRAEALSPIIESAAQRHAIDPHLVRAVIQVESGYAPRARSPKGAMGLMQLMPATAARF
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
GAASEDDILSPAVNVDVGVRYLRFLADRFGGRTDLVLAAYNAGEGAVIRHGYRVPPYRET
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCCCCCCCCCH
QDYVRKVLDLYPLAKR
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]