BacMap

Definition	Acidovorax citrulli AAC00-1 chromosome, complete genome.
Accession	NC_008752
Length	5,352,772

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The map label for this gene is dkgB [H]

Identifier: 120608990

GI number: 120608990

Start: 319244

End: 320077

Strand: Direct

Name: dkgB [H]

Synonym: Aave_0287

Alternate gene names: 120608990

Gene position: 319244-320077 (Clockwise)

Preceding gene: 120608989

Following gene: 120608992

Centisome position: 5.96

GC content: 69.9

Gene sequence:

>834_bases
ATGCAGGCATCCGCTTCCCCATCCACCCAACCGCCCGTGCCCCCGTTCGGCCTGGGCACGTTCCGGCTCAAGGGCCAGGC
CGCCATCGATTCGGTGGCGAATGCCCTCGACCTCGGCTATCGCCACGTGGACACCGCGCAGATCTACGGCAACGAAGCCG
AGGTGGGCCAGGCGGTTGCCGCCAGCGGCGTGGCCCGGGGCGACCTCTACCTCACCACCAAGGTCTGGACCGACCATCTC
GCCGCCAGCAGGCTCGTTCCCAGCCTGGAGGAAAGCCTCGAAAAGCTGCGCACGGATTACGTCGATCTGGCGCTGATCCA
CTGGCCTTCGCCCGGCGGCGCCGTGCCGCTGGCCGAGAGCCTGGAAGCGCTGGCCGATGCGCGGGCCCGCGGGCTCGTGC
GGCGCATCGGCGTATCGAACTTCACGGTCGCGCTGCTGCGCGAGGCGGTGGCTGCCGTGGGCGCGGAGTCGATCGCCACG
AACCAGATCGAACTGCACCCCTACCTGCAGAACCGGGCGGTGGTGGATTTCGCGCGCAGCCAGGGCATCCACATCACCTC
CTACATGACGCTGGGCTACGGCCAGATGCTGCAGGATGCGGAAATCCAGGCGATCGCGCAGGCATGCGGCGCCACGCCGG
CCCAGGTCGCGCTGGCCTGGGCAATGCAGTCGGGCTTCGCCGTGATTCCTTCGTCCACGCGCCGCGAACATCTGGCCTCG
AACCTGCGCGCCACGCAGTTGCGGCTGGAGGATGCGCACATGCAGCGCATCGCCGCGCTGGACCGGGGCGGCCGGCTGGT
GGACCCGGAAGGGCTGGCGCCGGCCTGGGACTGA

Upstream 100 bases:

>100_bases
GCGGCCGCGGGCCACTGAGGCGTGGGGCTGGCTCGGGCGCGCTTCTCCCTTTCCACCCACCCATCCATCCATTCATTCAT
TCACCGACAAGGAGTTTTCC

Downstream 100 bases:

>100_bases
CCCGGCGGGCGCCTGGAATCTGGGCTCAGATCGCCAGCTCGTAGCCCACCGTGATCGGCGCGTGGTCCGAGAACTTCTGC
TGCTTGTAGATGGACTCGGT

Product: 2,5-didehydrogluconate reductase

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MQASASPSTQPPVPPFGLGTFRLKGQAAIDSVANALDLGYRHVDTAQIYGNEAEVGQAVAASGVARGDLYLTTKVWTDHL
AASRLVPSLEESLEKLRTDYVDLALIHWPSPGGAVPLAESLEALADARARGLVRRIGVSNFTVALLREAVAAVGAESIAT
NQIELHPYLQNRAVVDFARSQGIHITSYMTLGYGQMLQDAEIQAIAQACGATPAQVALAWAMQSGFAVIPSSTRREHLAS
NLRATQLRLEDAHMQRIAALDRGGRLVDPEGLAPAWD

Sequences:

>Translated_277_residues
MQASASPSTQPPVPPFGLGTFRLKGQAAIDSVANALDLGYRHVDTAQIYGNEAEVGQAVAASGVARGDLYLTTKVWTDHL
AASRLVPSLEESLEKLRTDYVDLALIHWPSPGGAVPLAESLEALADARARGLVRRIGVSNFTVALLREAVAAVGAESIAT
NQIELHPYLQNRAVVDFARSQGIHITSYMTLGYGQMLQDAEIQAIAQACGATPAQVALAWAMQSGFAVIPSSTRREHLAS
NLRATQLRLEDAHMQRIAALDRGGRLVDPEGLAPAWD
>Mature_277_residues
MQASASPSTQPPVPPFGLGTFRLKGQAAIDSVANALDLGYRHVDTAQIYGNEAEVGQAVAASGVARGDLYLTTKVWTDHL
AASRLVPSLEESLEKLRTDYVDLALIHWPSPGGAVPLAESLEALADARARGLVRRIGVSNFTVALLREAVAAVGAESIAT
NQIELHPYLQNRAVVDFARSQGIHITSYMTLGYGQMLQDAEIQAIAQACGATPAQVALAWAMQSGFAVIPSSTRREHLAS
NLRATQLRLEDAHMQRIAALDRGGRLVDPEGLAPAWD

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=290, Percent_Identity=30.3448275862069, Blast_Score=151, Evalue=5e-37,
Organism=Homo sapiens, GI310109922, Length=274, Percent_Identity=31.3868613138686, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI45446745, Length=291, Percent_Identity=30.9278350515464, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI4503285, Length=291, Percent_Identity=30.9278350515464, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI310109920, Length=291, Percent_Identity=30.9278350515464, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI5453543, Length=291, Percent_Identity=30.5841924398625, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI24497585, Length=291, Percent_Identity=29.8969072164948, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI93277124, Length=299, Percent_Identity=30.7692307692308, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI5174695, Length=294, Percent_Identity=31.9727891156463, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI24497577, Length=289, Percent_Identity=29.0657439446367, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI5174391, Length=289, Percent_Identity=29.0657439446367, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI24497583, Length=297, Percent_Identity=29.96632996633, Blast_Score=141, Evalue=7e-34,
Organism=Homo sapiens, GI291291012, Length=271, Percent_Identity=29.8892988929889, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI4502049, Length=290, Percent_Identity=32.4137931034483, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI300116273, Length=272, Percent_Identity=31.9852941176471, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI300116271, Length=279, Percent_Identity=30.4659498207885, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI310109924, Length=159, Percent_Identity=30.188679245283, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI310109926, Length=104, Percent_Identity=37.5, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI207028673, Length=104, Percent_Identity=37.5, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1786400, Length=265, Percent_Identity=62.2641509433962, Blast_Score=347, Evalue=7e-97,
Organism=Escherichia coli, GI87082198, Length=261, Percent_Identity=35.2490421455939, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1788081, Length=268, Percent_Identity=28.7313432835821, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787674, Length=275, Percent_Identity=26.9090909090909, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI48994888, Length=283, Percent_Identity=27.208480565371, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17552492, Length=264, Percent_Identity=31.0606060606061, Blast_Score=147, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17537075, Length=289, Percent_Identity=31.8339100346021, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17537077, Length=288, Percent_Identity=30.5555555555556, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17550248, Length=295, Percent_Identity=32.2033898305085, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17537079, Length=294, Percent_Identity=28.9115646258503, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17561298, Length=274, Percent_Identity=28.4671532846715, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17566692, Length=282, Percent_Identity=31.2056737588652, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17538386, Length=275, Percent_Identity=31.6363636363636, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17561300, Length=273, Percent_Identity=29.3040293040293, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71998625, Length=261, Percent_Identity=30.6513409961686, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17562292, Length=270, Percent_Identity=31.8518518518519, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17564128, Length=293, Percent_Identity=28.6689419795222, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324694, Length=292, Percent_Identity=31.8493150684932, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320576, Length=285, Percent_Identity=31.2280701754386, Blast_Score=149, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6321896, Length=304, Percent_Identity=29.9342105263158, Blast_Score=142, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6322556, Length=257, Percent_Identity=28.0155642023346, Blast_Score=129, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6319625, Length=272, Percent_Identity=29.0441176470588, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6320079, Length=240, Percent_Identity=28.3333333333333, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24662789, Length=289, Percent_Identity=32.5259515570934, Blast_Score=148, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24644950, Length=301, Percent_Identity=31.8936877076412, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24657054, Length=267, Percent_Identity=32.2097378277154, Blast_Score=137, Evalue=6e-33,
Organism=Drosophila melanogaster, GI221378297, Length=321, Percent_Identity=30.5295950155763, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24662785, Length=289, Percent_Identity=33.9100346020761, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24662781, Length=290, Percent_Identity=34.8275862068965, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24663317, Length=285, Percent_Identity=29.8245614035088, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI20129731, Length=289, Percent_Identity=31.8339100346021, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21356425, Length=284, Percent_Identity=29.5774647887324, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI45553081, Length=285, Percent_Identity=27.719298245614, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI281366140, Length=260, Percent_Identity=26.5384615384615, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 29525; Mature: 29525

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQASASPSTQPPVPPFGLGTFRLKGQAAIDSVANALDLGYRHVDTAQIYGNEAEVGQAVA
CCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHH
ASGVARGDLYLTTKVWTDHLAASRLVPSLEESLEKLRTDYVDLALIHWPSPGGAVPLAES
HCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHH
LEALADARARGLVRRIGVSNFTVALLREAVAAVGAESIATNQIELHPYLQNRAVVDFARS
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHCCCEEEECHHHHCCHHHHHHHC
QGIHITSYMTLGYGQMLQDAEIQAIAQACGATPAQVALAWAMQSGFAVIPSSTRREHLAS
CCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEECCCCHHHHHHHH
NLRATQLRLEDAHMQRIAALDRGGRLVDPEGLAPAWD
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
>Mature Secondary Structure
MQASASPSTQPPVPPFGLGTFRLKGQAAIDSVANALDLGYRHVDTAQIYGNEAEVGQAVA
CCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHH
ASGVARGDLYLTTKVWTDHLAASRLVPSLEESLEKLRTDYVDLALIHWPSPGGAVPLAES
HCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHH
LEALADARARGLVRRIGVSNFTVALLREAVAAVGAESIATNQIELHPYLQNRAVVDFARS
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHCCCEEEECHHHHCCHHHHHHHC
QGIHITSYMTLGYGQMLQDAEIQAIAQACGATPAQVALAWAMQSGFAVIPSSTRREHLAS
CCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEECCCCHHHHHHHH
NLRATQLRLEDAHMQRIAALDRGGRLVDPEGLAPAWD
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796; 11108008 [H]