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The map label for this gene is cbiF [H]

Identifier: 120608779

GI number: 120608779

Start: 76275

End: 77156

Strand: Direct

Name: cbiF [H]

Synonym: Aave_0068

Alternate gene names: 120608779

Gene position: 76275-77156 (Clockwise)

Preceding gene: 120608777

Following gene: 120608780

Centisome position: 1.42

GC content: 72.22

Gene sequence:

>882_bases
ATGCCAGATTCTTCGGCGCAGGCCAACGGGGCCTGCGACACCGCCGTCCCGGGACCGGGCACCGTCTGGTTCGTCGGTGC
CGGGCCCGGCGATCCGGACCTCATCACCGTCAAGGGCCGCTCCCTGGTCGAACGCGCAGGAGCCATCCTGTTCGCCGGCT
CGCTCGTGGACGAAACCGCCACGCGCTGGGCGCCCCCGGGCTGCACCATCGCGGACAGCAAATCCATGACGCTGGACCAG
ATGGCCGCGTGGCTCGTGCTGCAGGCGCAGAAGCACCGCACCGTGGTGCGCCTGCAGACGGGCGATCCGGCGCTGTACGG
CGCGCTGGTCGAACTCGTGCAGCCCCTCGATGCCGCGGGCGTGCCGGTGCGCGTGGTGCCGGGCGTGTCCTCGGCCATGG
CCTCTGCCGCCGCGGCGGTGGAGAGTTTCACGCTGCCCGAGGTCACGCAGACCGTGGTGTTCACGCGCGTGGAAGGCCGC
ACGCCCATGCCGTCCGGAGAATCGCTCGCCGAACTCGCGCGCCACCGCTGCACGCTCTGCATCTTCCTGTCGATCACCCT
GCTGCACAAGGTGCAGGCCGGGCTGCGCGAAGCCGGCTGGCCGGACGACGCGCCGCTGCTCGTGGTCCACAAGGCCTCCT
GGCCCGGCGAGGAGCGCATCGTGCGCTGCACGGTGGCGGACGTGCGCGAGCGCTGCCGCGAGGCCCAGGTGGTGAGCCAG
GCCATGATCATCGCCAGCCCCACGCTGGGCGCGCGCCACTGGCCCGAACTCGCCAGATCCAAGCTCTACGACCCGGGCTT
CACCCACCGCTTCCGGCGCGCGGCCGACGTACCCGCCGCCGCCCCGCAAGCCGCCACCAACACGCCGGAACCCCAACGAT
GA

Upstream 100 bases:

>100_bases
GCGCGCGCTTTTGCGTGCATGCTCCATTTCACGGACCCTGCGGGGAGGCAGGGTGCGGGCGCGCCTGCCGCGGCGGGCGC
ATCTTTGTTCATCGCTTCCC

Downstream 100 bases:

>100_bases
CGCACCTCCACGCTCCGCATTCCCACTCCCACGGCCACGCCGCCCACGGCGGCGACCACGTCCAGGCTGCCCTGAGCGCG
CCCGTGCAGGCCGGAACCAC

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MPDSSAQANGACDTAVPGPGTVWFVGAGPGDPDLITVKGRSLVERAGAILFAGSLVDETATRWAPPGCTIADSKSMTLDQ
MAAWLVLQAQKHRTVVRLQTGDPALYGALVELVQPLDAAGVPVRVVPGVSSAMASAAAAVESFTLPEVTQTVVFTRVEGR
TPMPSGESLAELARHRCTLCIFLSITLLHKVQAGLREAGWPDDAPLLVVHKASWPGEERIVRCTVADVRERCREAQVVSQ
AMIIASPTLGARHWPELARSKLYDPGFTHRFRRAADVPAAAPQAATNTPEPQR

Sequences:

>Translated_293_residues
MPDSSAQANGACDTAVPGPGTVWFVGAGPGDPDLITVKGRSLVERAGAILFAGSLVDETATRWAPPGCTIADSKSMTLDQ
MAAWLVLQAQKHRTVVRLQTGDPALYGALVELVQPLDAAGVPVRVVPGVSSAMASAAAAVESFTLPEVTQTVVFTRVEGR
TPMPSGESLAELARHRCTLCIFLSITLLHKVQAGLREAGWPDDAPLLVVHKASWPGEERIVRCTVADVRERCREAQVVSQ
AMIIASPTLGARHWPELARSKLYDPGFTHRFRRAADVPAAAPQAATNTPEPQR
>Mature_292_residues
PDSSAQANGACDTAVPGPGTVWFVGAGPGDPDLITVKGRSLVERAGAILFAGSLVDETATRWAPPGCTIADSKSMTLDQM
AAWLVLQAQKHRTVVRLQTGDPALYGALVELVQPLDAAGVPVRVVPGVSSAMASAAAAVESFTLPEVTQTVVFTRVEGRT
PMPSGESLAELARHRCTLCIFLSITLLHKVQAGLREAGWPDDAPLLVVHKASWPGEERIVRCTVADVRERCREAQVVSQA
MIIASPTLGARHWPELARSKLYDPGFTHRFRRAADVPAAAPQAATNTPEPQR

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=238, Percent_Identity=26.4705882352941, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 31137; Mature: 31006

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDSSAQANGACDTAVPGPGTVWFVGAGPGDPDLITVKGRSLVERAGAILFAGSLVDETA
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCHHHHHHCCCEEEECHHHHHHH
TRWAPPGCTIADSKSMTLDQMAAWLVLQAQKHRTVVRLQTGDPALYGALVELVQPLDAAG
HHCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCC
VPVRVVPGVSSAMASAAAAVESFTLPEVTQTVVFTRVEGRTPMPSGESLAELARHRCTLC
CCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHH
IFLSITLLHKVQAGLREAGWPDDAPLLVVHKASWPGEERIVRCTVADVRERCREAQVVSQ
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHEEEEEHHHHHHHHHHHHHHHH
AMIIASPTLGARHWPELARSKLYDPGFTHRFRRAADVPAAAPQAATNTPEPQR
HHHEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PDSSAQANGACDTAVPGPGTVWFVGAGPGDPDLITVKGRSLVERAGAILFAGSLVDETA
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCHHHHHHCCCEEEECHHHHHHH
TRWAPPGCTIADSKSMTLDQMAAWLVLQAQKHRTVVRLQTGDPALYGALVELVQPLDAAG
HHCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCC
VPVRVVPGVSSAMASAAAAVESFTLPEVTQTVVFTRVEGRTPMPSGESLAELARHRCTLC
CCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHH
IFLSITLLHKVQAGLREAGWPDDAPLLVVHKASWPGEERIVRCTVADVRERCREAQVVSQ
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHEEEEEHHHHHHHHHHHHHHHH
AMIIASPTLGARHWPELARSKLYDPGFTHRFRRAADVPAAAPQAATNTPEPQR
HHHEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]