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The map label for this gene is leuA [H]

Identifier: 120608732

GI number: 120608732

Start: 19177

End: 20940

Strand: Direct

Name: leuA [H]

Synonym: Aave_0019

Alternate gene names: 120608732

Gene position: 19177-20940 (Clockwise)

Preceding gene: 120608729

Following gene: 120608733

Centisome position: 0.36

GC content: 69.5

Gene sequence:

>1764_bases
ATGGCCTGCCCGGCACGGCACCGTTTCGTGGAGCCCGTCATGATGATTTCCAAGCCCGCCACCAAGTACCAGCCCGCCAC
CAAGTACCAGCCCACCGCCAGCGTGTCCCTGCCCGACCGCACGTGGCCCACCCGCAGCATCACGCAGGCGCCGATCTGGC
TTTCCACCGACCTGCGCGACGGCAACCAGGCGCTCTTCGAGCCGATGAACGGCGAGCGCAAGATGCGACTCTTCGAGGAA
CTCGTGCGCATCGGCTTCAAGGAAATCGAGGTGGGCTTCCCTGCTGCGTCGCAGACGGATTTCGATTTCGTGCGCCGCCT
GATCGAGGAAAACCGCATTCCCGACGACGTGACCATCATGGTCATGACCCAGTCGCGCGAAGACCTGATCGAGCGCACGG
TGCAGGCCGTGCAGGGCGCGCCGCGCGCCATCGTGCACCTCTACAACGCCACGGCACCGGCCTGGCGCCGCATCGTGTTC
GGAATGAACGTGTCGCAGGTGATGGCGTTCATCGGGCACCACGTGTCGCTCCTCAAGCGCCTCACCGACGCACAGCCGGC
CACCGCCTGGACGCTGCAGTATTCGCCCGAGACATTCAGCGCCACCGAGCCCGAAGTGTCGCTGCGCGCATGCCAGACGG
CGATCGCGGCCTGGGGCGCCGGCCCGGGCCGGCCCATCATCATCAACCTGCCGACCACGGTGGAGAACGCCACGCCCAAC
GTGTTCGCCGACCAGATCGAATGGATGCACCGCCGTCTCGCGCCGCGCGAGCACATCGTGCTTTCCGTGCACCCCCACAA
CGACCGCGGCACGGGCGTGGCCGCGGCCGAGCTGGCGATGATGGCCGGCGCCGACCGCGTGGAAGGGTGCCTCCTCGGCA
ATGGCGAGCGCTGCGGCAACGTGGACATCGTGACGCTCGCGCTCAACATGTACACGCAGGGCGTGCACCCGAACCTCGAT
TTCTCCGACATCACGCACGTGGCGCGCGTGGCCGAGGAATGCACCTCGCTGCCGGTGCATCCACGCCATCCGTATGCGGG
CGACCTCGTCTTCACCGCGTTCTCGGGTTCGCACCAGGACGCGATCAAGAAGGGCTTCGCGGCGCAGGACCCGGCGGGCC
TCTGGGAAGTGCCCTACCTCCCGATCGATCCGGCGGACCTCGGCCGCACCTACGACAGCGTGATCCGCGTGAACAGCCAG
TCGGGCAAGGGGGGCATCGCCTTCCTGCTGGAGCGCGAACGGGGCGTGGTGATGCCGCGCCGGCTGCAGGTGGAGTTCAG
CGCCGTGGTGCAGCGCGCCACGGACGCGAGCGAGGGCGAGATGGACGGCGATGCGCTCTGGAGCCTGTTCTCGCAGACCT
ACATCGCCGCACCGGCGCAGGGCACGCCCGGCGCGCTCACGCTGCATGGGCAGCGCCTGGATGAAGACGGCCAGGGCATC
TCGCTGGACGTGACCATCGACGGCGTGCGCCAGACCCTGCAGGGCCGGGGCAACGGTCCGATCGACGCCACGGTGGACGC
CATCGGCCTGCCGTTGCGCGTGGACCACTACGAAGAGCGCGCCACCGGCGCGGGGGCCGGCGCCCAGGCGCTGGCGATCG
TGGAGGCCGCGCTCGAGGGCGTGCCGGGCGCGACCTTCGGCGTCGGGCTGGACCACAGCATCGTCAGTGCGTCGGTGCAG
GCCGTGGTGGCGGTGACCAACCGGCTGATCGCGCGGCGCGGCGCGAAGGCCGACGCGGTACAGGCAGCTCCGGCGACGGC
CTGA

Upstream 100 bases:

>100_bases
GTGGAGGGCTGCAGGGAACGTCGCACCGGTCCTTTCTTCCGTTGATTGTTTCAGCCCTGCCGTGGCGGCCACCTGCCCCA
CATACCTTCGCCCCGCGGCG

Downstream 100 bases:

>100_bases
TATCCGCACCGCGGCGCGGGGGCGCCGCTTGGACGGAGCGAGGGCAGGGCGCGGATCGGAAGCGGCCTGCGCCGTGCCAC
GCCGTTGCCACGGCATGGCC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MACPARHRFVEPVMMISKPATKYQPATKYQPTASVSLPDRTWPTRSITQAPIWLSTDLRDGNQALFEPMNGERKMRLFEE
LVRIGFKEIEVGFPAASQTDFDFVRRLIEENRIPDDVTIMVMTQSREDLIERTVQAVQGAPRAIVHLYNATAPAWRRIVF
GMNVSQVMAFIGHHVSLLKRLTDAQPATAWTLQYSPETFSATEPEVSLRACQTAIAAWGAGPGRPIIINLPTTVENATPN
VFADQIEWMHRRLAPREHIVLSVHPHNDRGTGVAAAELAMMAGADRVEGCLLGNGERCGNVDIVTLALNMYTQGVHPNLD
FSDITHVARVAEECTSLPVHPRHPYAGDLVFTAFSGSHQDAIKKGFAAQDPAGLWEVPYLPIDPADLGRTYDSVIRVNSQ
SGKGGIAFLLERERGVVMPRRLQVEFSAVVQRATDASEGEMDGDALWSLFSQTYIAAPAQGTPGALTLHGQRLDEDGQGI
SLDVTIDGVRQTLQGRGNGPIDATVDAIGLPLRVDHYEERATGAGAGAQALAIVEAALEGVPGATFGVGLDHSIVSASVQ
AVVAVTNRLIARRGAKADAVQAAPATA

Sequences:

>Translated_587_residues
MACPARHRFVEPVMMISKPATKYQPATKYQPTASVSLPDRTWPTRSITQAPIWLSTDLRDGNQALFEPMNGERKMRLFEE
LVRIGFKEIEVGFPAASQTDFDFVRRLIEENRIPDDVTIMVMTQSREDLIERTVQAVQGAPRAIVHLYNATAPAWRRIVF
GMNVSQVMAFIGHHVSLLKRLTDAQPATAWTLQYSPETFSATEPEVSLRACQTAIAAWGAGPGRPIIINLPTTVENATPN
VFADQIEWMHRRLAPREHIVLSVHPHNDRGTGVAAAELAMMAGADRVEGCLLGNGERCGNVDIVTLALNMYTQGVHPNLD
FSDITHVARVAEECTSLPVHPRHPYAGDLVFTAFSGSHQDAIKKGFAAQDPAGLWEVPYLPIDPADLGRTYDSVIRVNSQ
SGKGGIAFLLERERGVVMPRRLQVEFSAVVQRATDASEGEMDGDALWSLFSQTYIAAPAQGTPGALTLHGQRLDEDGQGI
SLDVTIDGVRQTLQGRGNGPIDATVDAIGLPLRVDHYEERATGAGAGAQALAIVEAALEGVPGATFGVGLDHSIVSASVQ
AVVAVTNRLIARRGAKADAVQAAPATA
>Mature_586_residues
ACPARHRFVEPVMMISKPATKYQPATKYQPTASVSLPDRTWPTRSITQAPIWLSTDLRDGNQALFEPMNGERKMRLFEEL
VRIGFKEIEVGFPAASQTDFDFVRRLIEENRIPDDVTIMVMTQSREDLIERTVQAVQGAPRAIVHLYNATAPAWRRIVFG
MNVSQVMAFIGHHVSLLKRLTDAQPATAWTLQYSPETFSATEPEVSLRACQTAIAAWGAGPGRPIIINLPTTVENATPNV
FADQIEWMHRRLAPREHIVLSVHPHNDRGTGVAAAELAMMAGADRVEGCLLGNGERCGNVDIVTLALNMYTQGVHPNLDF
SDITHVARVAEECTSLPVHPRHPYAGDLVFTAFSGSHQDAIKKGFAAQDPAGLWEVPYLPIDPADLGRTYDSVIRVNSQS
GKGGIAFLLERERGVVMPRRLQVEFSAVVQRATDASEGEMDGDALWSLFSQTYIAAPAQGTPGALTLHGQRLDEDGQGIS
LDVTIDGVRQTLQGRGNGPIDATVDAIGLPLRVDHYEERATGAGAGAQALAIVEAALEGVPGATFGVGLDHSIVSASVQA
VVAVTNRLIARRGAKADAVQAAPATA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=551, Percent_Identity=25.2268602540835, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324225, Length=580, Percent_Identity=46.2068965517241, Blast_Score=521, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6324682, Length=581, Percent_Identity=45.4388984509466, Blast_Score=515, Evalue=1e-147,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 63472; Mature: 63341

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS ; PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACPARHRFVEPVMMISKPATKYQPATKYQPTASVSLPDRTWPTRSITQAPIWLSTDLRD
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
GNQALFEPMNGERKMRLFEELVRIGFKEIEVGFPAASQTDFDFVRRLIEENRIPDDVTIM
CCHHHHCCCCCHHHHHHHHHHHHCCHHEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEE
VMTQSREDLIERTVQAVQGAPRAIVHLYNATAPAWRRIVFGMNVSQVMAFIGHHVSLLKR
EEECCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LTDAQPATAWTLQYSPETFSATEPEVSLRACQTAIAAWGAGPGRPIIINLPTTVENATPN
HHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
VFADQIEWMHRRLAPREHIVLSVHPHNDRGTGVAAAELAMMAGADRVEGCLLGNGERCGN
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCHHCEEEECCCCCCCC
VDIVTLALNMYTQGVHPNLDFSDITHVARVAEECTSLPVHPRHPYAGDLVFTAFSGSHQD
EEEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHH
AIKKGFAAQDPAGLWEVPYLPIDPADLGRTYDSVIRVNSQSGKGGIAFLLERERGVVMPR
HHHHCCCCCCCCCCEECCCCCCCHHHHCCCHHHHEEEECCCCCCCEEEEEECCCCCCCCH
RLQVEFSAVVQRATDASEGEMDGDALWSLFSQTYIAAPAQGTPGALTLHGQRLDEDGQGI
HEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCEEEEECCCCCCCCCCE
SLDVTIDGVRQTLQGRGNGPIDATVDAIGLPLRVDHYEERATGAGAGAQALAIVEAALEG
EEEEEEHHHHHHHCCCCCCCCCCCHHHCCCCEEECCHHHHCCCCCCCHHHHHHHHHHHHC
VPGATFGVGLDHSIVSASVQAVVAVTNRLIARRGAKADAVQAAPATA
CCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCC
>Mature Secondary Structure 
ACPARHRFVEPVMMISKPATKYQPATKYQPTASVSLPDRTWPTRSITQAPIWLSTDLRD
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
GNQALFEPMNGERKMRLFEELVRIGFKEIEVGFPAASQTDFDFVRRLIEENRIPDDVTIM
CCHHHHCCCCCHHHHHHHHHHHHCCHHEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEE
VMTQSREDLIERTVQAVQGAPRAIVHLYNATAPAWRRIVFGMNVSQVMAFIGHHVSLLKR
EEECCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LTDAQPATAWTLQYSPETFSATEPEVSLRACQTAIAAWGAGPGRPIIINLPTTVENATPN
HHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
VFADQIEWMHRRLAPREHIVLSVHPHNDRGTGVAAAELAMMAGADRVEGCLLGNGERCGN
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCHHCEEEECCCCCCCC
VDIVTLALNMYTQGVHPNLDFSDITHVARVAEECTSLPVHPRHPYAGDLVFTAFSGSHQD
EEEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHH
AIKKGFAAQDPAGLWEVPYLPIDPADLGRTYDSVIRVNSQSGKGGIAFLLERERGVVMPR
HHHHCCCCCCCCCCEECCCCCCCHHHHCCCHHHHEEEECCCCCCCEEEEEECCCCCCCCH
RLQVEFSAVVQRATDASEGEMDGDALWSLFSQTYIAAPAQGTPGALTLHGQRLDEDGQGI
HEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCEEEEECCCCCCCCCCE
SLDVTIDGVRQTLQGRGNGPIDATVDAIGLPLRVDHYEERATGAGAGAQALAIVEAALEG
EEEEEEHHHHHHHCCCCCCCCCCCHHHCCCCEEECCHHHHCCCCCCCHHHHHHHHHHHHC
VPGATFGVGLDHSIVSASVQAVVAVTNRLIARRGAKADAVQAAPATA
CCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA