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The map label for this gene is yafC [C]

Identifier: 119947131

GI number: 119947131

Start: 4347292

End: 4348188

Strand: Reverse

Name: yafC [C]

Synonym: Ping_3529

Alternate gene names: 119947131

Gene position: 4348188-4347292 (Counterclockwise)

Preceding gene: 119947134

Following gene: 119947127

Centisome position: 95.36

GC content: 42.59

Gene sequence:

>897_bases
ATGCAATGGCAGGGTATTAATGAGTTTGTTGCGGTCGCAGAGATGCACAGCTTTACCCTGGCGGGTAAAAAATTGAAGAT
TTCGACCGCGCAGGTCAGCAGACAAATTAACGCATTAGAAAAAAGGTTGAATGTCAGGCTGCTCTACCGCACTACACGTC
ATGTTTCAGTGACGCAAGAGGGGCAGATTTTCTATCAGCATTGCCATAATGTATTAGAGGGGTTGGCAGCTGCGGAGCAG
GCAATTAGCAGTTTACAGGCTAAACCACAGGGGCATATTAAACTCTCAGCCCCTATTACTTATGGAGAACAAAAAATTCT
GCCGTTAATTAACGATTTTTTGTGCCGTTATGAGAGGATCCAAGTGACCTGCGAGCTGACTAATCAGCGCGTTGATTTAG
TTGATGGCGGATTTGATTTAGCCATTCGCCTCGGTGACTTACCGGATTCGAGTTTAGTCGCCAAAAAATTAGCGGCACGT
GAAAATTTTGTGTGCGCATCACCTGAGTACCTTAAAAAATTTGGTGAACCACACTCATTATCTGAATTAGTGCAGCACAA
TTGTTTATTAGGCACGCGTGATTATTGGCATTTTACTGAACAGGGCAAAGAAAAAAATATTCGTGTGCGGGGGAATCTGC
GTTGTAATAGTGGCTTTGGGCTAGTGGATGCTGCGCTGAAAAATATTGGTATTATTCAGCTGCCTGATTATTATCTGCAG
GAGCATATTAATTCAGGCAAATTAATTTCTTTATTAAACAATGTCCGGGTTGCTGAGGAGGGGATATGGGCGGTTTACCC
GCAAAATCGTTATTTATCCCCAAAAATTCGTTTGCTTATTGATTTTCTTGCTGAGCATTTTGCAGACGGGACAAAACATC
ACTTAATGAAGGCTTAA

Upstream 100 bases:

>100_bases
TGAGTGTTACTGTTTTATTTAACTTCCCCCTATTATAACTATTACTGTTTGAATGATAATATAGTTAAAATTAAAATAAC
TTTTACTGTGTGGTAACAAT

Downstream 100 bases:

>100_bases
CTTGATGCCCTAAAGCCAGCGTTTCTGGGCAGGGAGTAACCAGATCCGGCACCTGAAAAACATGCATGCCAGCATTCACG
GCTGCCCGAACACCATGATT

Product: transcriptional regulator, substrate-binding of LysR family protein

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MQWQGINEFVAVAEMHSFTLAGKKLKISTAQVSRQINALEKRLNVRLLYRTTRHVSVTQEGQIFYQHCHNVLEGLAAAEQ
AISSLQAKPQGHIKLSAPITYGEQKILPLINDFLCRYERIQVTCELTNQRVDLVDGGFDLAIRLGDLPDSSLVAKKLAAR
ENFVCASPEYLKKFGEPHSLSELVQHNCLLGTRDYWHFTEQGKEKNIRVRGNLRCNSGFGLVDAALKNIGIIQLPDYYLQ
EHINSGKLISLLNNVRVAEEGIWAVYPQNRYLSPKIRLLIDFLAEHFADGTKHHLMKA

Sequences:

>Translated_298_residues
MQWQGINEFVAVAEMHSFTLAGKKLKISTAQVSRQINALEKRLNVRLLYRTTRHVSVTQEGQIFYQHCHNVLEGLAAAEQ
AISSLQAKPQGHIKLSAPITYGEQKILPLINDFLCRYERIQVTCELTNQRVDLVDGGFDLAIRLGDLPDSSLVAKKLAAR
ENFVCASPEYLKKFGEPHSLSELVQHNCLLGTRDYWHFTEQGKEKNIRVRGNLRCNSGFGLVDAALKNIGIIQLPDYYLQ
EHINSGKLISLLNNVRVAEEGIWAVYPQNRYLSPKIRLLIDFLAEHFADGTKHHLMKA
>Mature_298_residues
MQWQGINEFVAVAEMHSFTLAGKKLKISTAQVSRQINALEKRLNVRLLYRTTRHVSVTQEGQIFYQHCHNVLEGLAAAEQ
AISSLQAKPQGHIKLSAPITYGEQKILPLINDFLCRYERIQVTCELTNQRVDLVDGGFDLAIRLGDLPDSSLVAKKLAAR
ENFVCASPEYLKKFGEPHSLSELVQHNCLLGTRDYWHFTEQGKEKNIRVRGNLRCNSGFGLVDAALKNIGIIQLPDYYLQ
EHINSGKLISLLNNVRVAEEGIWAVYPQNRYLSPKIRLLIDFLAEHFADGTKHHLMKA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=283, Percent_Identity=34.2756183745583, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1789440, Length=281, Percent_Identity=31.6725978647687, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI145693193, Length=282, Percent_Identity=32.2695035460993, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87081978, Length=286, Percent_Identity=31.8181818181818, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=27.681660899654, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1787589, Length=282, Percent_Identity=29.0780141843972, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789639, Length=285, Percent_Identity=27.3684210526316, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788706, Length=298, Percent_Identity=28.5234899328859, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786448, Length=269, Percent_Identity=24.907063197026, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789173, Length=259, Percent_Identity=25.4826254826255, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI157672245, Length=133, Percent_Identity=32.3308270676692, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790011, Length=299, Percent_Identity=22.4080267558528, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33745; Mature: 33745

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQWQGINEFVAVAEMHSFTLAGKKLKISTAQVSRQINALEKRLNVRLLYRTTRHVSVTQE
CCCCCHHHHHHHHHHHHHEECCCEEEEHHHHHHHHHHHHHHHHCEEEEEEECCEEEECCC
GQIFYQHCHNVLEGLAAAEQAISSLQAKPQGHIKLSAPITYGEQKILPLINDFLCRYERI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEECCCCHHHHHHHHHHHHHHHE
QVTCELTNQRVDLVDGGFDLAIRLGDLPDSSLVAKKLAARENFVCASPEYLKKFGEPHSL
EEEEEECCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHHCCCEECCHHHHHHCCCCCCH
SELVQHNCLLGTRDYWHFTEQGKEKNIRVRGNLRCNSGFGLVDAALKNIGIIQLPDYYLQ
HHHHHHCCCCCCCHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHCCEEECCHHHHH
EHINSGKLISLLNNVRVAEEGIWAVYPQNRYLSPKIRLLIDFLAEHFADGTKHHLMKA
HHCCCCHHHHHHHHCEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCC
>Mature Secondary Structure
MQWQGINEFVAVAEMHSFTLAGKKLKISTAQVSRQINALEKRLNVRLLYRTTRHVSVTQE
CCCCCHHHHHHHHHHHHHEECCCEEEEHHHHHHHHHHHHHHHHCEEEEEEECCEEEECCC
GQIFYQHCHNVLEGLAAAEQAISSLQAKPQGHIKLSAPITYGEQKILPLINDFLCRYERI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEECCCCHHHHHHHHHHHHHHHE
QVTCELTNQRVDLVDGGFDLAIRLGDLPDSSLVAKKLAARENFVCASPEYLKKFGEPHSL
EEEEEECCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHHCCCEECCHHHHHHCCCCCCH
SELVQHNCLLGTRDYWHFTEQGKEKNIRVRGNLRCNSGFGLVDAALKNIGIIQLPDYYLQ
HHHHHHCCCCCCCHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHHHHHCCEEECCHHHHH
EHINSGKLISLLNNVRVAEEGIWAVYPQNRYLSPKIRLLIDFLAEHFADGTKHHLMKA
HHCCCCHHHHHHHHCEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]