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The map label for this gene is opuE [H]

Identifier: 119947092

GI number: 119947092

Start: 4318272

End: 4319768

Strand: Reverse

Name: opuE [H]

Synonym: Ping_3490

Alternate gene names: 119947092

Gene position: 4319768-4318272 (Counterclockwise)

Preceding gene: 119947093

Following gene: 119947091

Centisome position: 94.74

GC content: 43.15

Gene sequence:

>1497_bases
ATGGCTGACAACACTTTTGCCATAGTATCGACATTTCTAGTGTATCTGGTGGTGATGTTACTCATCGGTTATTACGCATA
TAAACGCACCAGCGATACGTCGGATTATTTTTTAGGCGGACGTACATTAGGTCCATGGCCTGCAGCATTATCTGCCGGTG
CTTCGGATATGAGTGGCTGGTTATTACTGGGTTTGCCTGGTTACGCTTATGCAGCGGGCATGGAGTCTTTATGGCTCGCT
GGTGGACTGTTAATTGGTAGCTGGGCTAACTGGCTGATCACTGCAAAACGTCTGCGTACTTATAGTATTACGGCCGATGA
TGCATTAACTTTGCCGGAATTTATTGCCCGTCGTTTCAATGACAAAACGAAATTGCTGCAAACCCTTTCCGCTTTTTTCA
TCCTGATGTTCTTCCTCTTTTATACCAGCTCAGGTTTGGTTGCCGGTGGTAAATTATTTGAAACTGTCTTTGGTCTAGAT
TATATCTATGCAGTAATTATCGGTGCTATCTGTATTGTTTCTTATACTCTATTTGGCGGGTTTTTGGCCGTCTCATGGAC
AGATTTAGTGCAGGGCTTATTAATGTCAGCAGCACTAGTGATTGTACCTATCGTCGCAATGCAGGGCGGATTCGATCAGC
TAAGCACTAATCTAGAAAATATTAATCCGGAACTACTTACCCTATGGAATGGTATGGATGGTGAGCCGTTATCCTGGATT
GCGATTATCTCATTAGTCGCCTGGGGTCTGGGTTATTTTGGCCAACCGCATATTCTTGCGCGTTTTCTAGCAACACGCAC
AAATAAAGATTTAACTAAAGCACGCCGTATCGCGGTGAGCTGGACTATTATCTCTATCTTTGGTTCGGTTATGGTGGGTA
TTGTTGGTCTGGTCTTTGTGCAAAATCAGGTGGGCATGCAAGTCGCTGATGGCGAAAAGATCTTTATGGTGTTAGTTAAT
GCTGTCTTTCATCCGATCCCTGCCGGTATTTTGTTAGCCGCTATTCTTGCCGCTATTATGAGCACCGCTGATTCGCAGTT
ATTAGTCTCCTCTTCAGCATTAGCAGAAGACTTTTACAAGCAACTTCTCCGCCCTCAAGCTTCGTCAAAAGAAGTGTTGA
TGGTTGGACGTATCGGTGTAATTCTATTAGCACTATTAGCCTTGTATTTCGCAATGGATCCTGATAGTTCGGTACTTAGC
TTAGTTTCCTATGCCTGGGCTGGTTTCGGTGCTGCATTTGGTCCGGTATTAATATTAAGCATATACTGGAAACGAATGAA
CGGAAAGGGTGCTTTAGCAGGTCTTCTTGTCGGTGGTATCACTATTGTTGTCTGGAAGCAGTTAACAGGTGGTATTTTTG
AAGTGTATGAGATTGTACCGGGAATAATTTTTGCTTCTGCGGCAATTGTTATCACCAGTTTAGTCACTGCTCCGCCGGAA
AAAGCGGTAACAGATAAGTTTGAAGAATTTGAAAGAAACTTAAAAACATTTAAATAA

Upstream 100 bases:

>100_bases
CCTATTGTTTGTTATGTAAAAAATTTAAAGAATAAAATATTTTATCTTGTTATAGTGCAGTTTATTTTTTAACTTTAACT
TTAATATTAATGGGAGTTTC

Downstream 100 bases:

>100_bases
GAAGCAGACAGCTCTAAGGTCTGAAGGCTGGAAGGTCTGGAGGCTTGAAGCTCTAAAAGATAAAAGGCTGTAACTTTGTA
TAAAGTTACAGCCTTTTTTT

Product: sodium/proline symporter

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MADNTFAIVSTFLVYLVVMLLIGYYAYKRTSDTSDYFLGGRTLGPWPAALSAGASDMSGWLLLGLPGYAYAAGMESLWLA
GGLLIGSWANWLITAKRLRTYSITADDALTLPEFIARRFNDKTKLLQTLSAFFILMFFLFYTSSGLVAGGKLFETVFGLD
YIYAVIIGAICIVSYTLFGGFLAVSWTDLVQGLLMSAALVIVPIVAMQGGFDQLSTNLENINPELLTLWNGMDGEPLSWI
AIISLVAWGLGYFGQPHILARFLATRTNKDLTKARRIAVSWTIISIFGSVMVGIVGLVFVQNQVGMQVADGEKIFMVLVN
AVFHPIPAGILLAAILAAIMSTADSQLLVSSSALAEDFYKQLLRPQASSKEVLMVGRIGVILLALLALYFAMDPDSSVLS
LVSYAWAGFGAAFGPVLILSIYWKRMNGKGALAGLLVGGITIVVWKQLTGGIFEVYEIVPGIIFASAAIVITSLVTAPPE
KAVTDKFEEFERNLKTFK

Sequences:

>Translated_498_residues
MADNTFAIVSTFLVYLVVMLLIGYYAYKRTSDTSDYFLGGRTLGPWPAALSAGASDMSGWLLLGLPGYAYAAGMESLWLA
GGLLIGSWANWLITAKRLRTYSITADDALTLPEFIARRFNDKTKLLQTLSAFFILMFFLFYTSSGLVAGGKLFETVFGLD
YIYAVIIGAICIVSYTLFGGFLAVSWTDLVQGLLMSAALVIVPIVAMQGGFDQLSTNLENINPELLTLWNGMDGEPLSWI
AIISLVAWGLGYFGQPHILARFLATRTNKDLTKARRIAVSWTIISIFGSVMVGIVGLVFVQNQVGMQVADGEKIFMVLVN
AVFHPIPAGILLAAILAAIMSTADSQLLVSSSALAEDFYKQLLRPQASSKEVLMVGRIGVILLALLALYFAMDPDSSVLS
LVSYAWAGFGAAFGPVLILSIYWKRMNGKGALAGLLVGGITIVVWKQLTGGIFEVYEIVPGIIFASAAIVITSLVTAPPE
KAVTDKFEEFERNLKTFK
>Mature_497_residues
ADNTFAIVSTFLVYLVVMLLIGYYAYKRTSDTSDYFLGGRTLGPWPAALSAGASDMSGWLLLGLPGYAYAAGMESLWLAG
GLLIGSWANWLITAKRLRTYSITADDALTLPEFIARRFNDKTKLLQTLSAFFILMFFLFYTSSGLVAGGKLFETVFGLDY
IYAVIIGAICIVSYTLFGGFLAVSWTDLVQGLLMSAALVIVPIVAMQGGFDQLSTNLENINPELLTLWNGMDGEPLSWIA
IISLVAWGLGYFGQPHILARFLATRTNKDLTKARRIAVSWTIISIFGSVMVGIVGLVFVQNQVGMQVADGEKIFMVLVNA
VFHPIPAGILLAAILAAIMSTADSQLLVSSSALAEDFYKQLLRPQASSKEVLMVGRIGVILLALLALYFAMDPDSSVLSL
VSYAWAGFGAAFGPVLILSIYWKRMNGKGALAGLLVGGITIVVWKQLTGGIFEVYEIVPGIIFASAAIVITSLVTAPPEK
AVTDKFEEFERNLKTFK

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=494, Percent_Identity=45.748987854251, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI14140236, Length=477, Percent_Identity=23.4800838574423, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI4507035, Length=365, Percent_Identity=27.1232876712329, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI110835708, Length=536, Percent_Identity=23.3208955223881, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI109659836, Length=527, Percent_Identity=24.6679316888046, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI4507031, Length=480, Percent_Identity=22.5, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI17941285, Length=471, Percent_Identity=25.4777070063694, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI109659839, Length=543, Percent_Identity=24.1252302025783, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI4507033, Length=410, Percent_Identity=23.1707317073171, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI167466278, Length=452, Percent_Identity=26.1061946902655, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1787251, Length=491, Percent_Identity=47.8615071283096, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI87082237, Length=493, Percent_Identity=30.8316430020284, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1790503, Length=465, Percent_Identity=26.4516129032258, Blast_Score=131, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17539284, Length=467, Percent_Identity=25.4817987152034, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24651741, Length=452, Percent_Identity=23.8938053097345, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI221459584, Length=373, Percent_Identity=24.3967828418231, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221459588, Length=507, Percent_Identity=25.0493096646943, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221459586, Length=444, Percent_Identity=22.2972972972973, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281362918, Length=244, Percent_Identity=26.2295081967213, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 53910; Mature: 53779

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNTFAIVSTFLVYLVVMLLIGYYAYKRTSDTSDYFLGGRTLGPWPAALSAGASDMSGW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHCCCCCCCCE
LLLGLPGYAYAAGMESLWLAGGLLIGSWANWLITAKRLRTYSITADDALTLPEFIARRFN
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHC
DKTKLLQTLSAFFILMFFLFYTSSGLVAGGKLFETVFGLDYIYAVIIGAICIVSYTLFGG
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLAVSWTDLVQGLLMSAALVIVPIVAMQGGFDQLSTNLENINPELLTLWNGMDGEPLSWI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHCCCHHEEEECCCCCCCHHHH
AIISLVAWGLGYFGQPHILARFLATRTNKDLTKARRIAVSWTIISIFGSVMVGIVGLVFV
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QNQVGMQVADGEKIFMVLVNAVFHPIPAGILLAAILAAIMSTADSQLLVSSSALAEDFYK
HHHCCCEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QLLRPQASSKEVLMVGRIGVILLALLALYFAMDPDSSVLSLVSYAWAGFGAAFGPVLILS
HHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IYWKRMNGKGALAGLLVGGITIVVWKQLTGGIFEVYEIVPGIIFASAAIVITSLVTAPPE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
KAVTDKFEEFERNLKTFK
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ADNTFAIVSTFLVYLVVMLLIGYYAYKRTSDTSDYFLGGRTLGPWPAALSAGASDMSGW
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHCCCCCCCCE
LLLGLPGYAYAAGMESLWLAGGLLIGSWANWLITAKRLRTYSITADDALTLPEFIARRFN
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHC
DKTKLLQTLSAFFILMFFLFYTSSGLVAGGKLFETVFGLDYIYAVIIGAICIVSYTLFGG
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLAVSWTDLVQGLLMSAALVIVPIVAMQGGFDQLSTNLENINPELLTLWNGMDGEPLSWI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHCCCHHEEEECCCCCCCHHHH
AIISLVAWGLGYFGQPHILARFLATRTNKDLTKARRIAVSWTIISIFGSVMVGIVGLVFV
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QNQVGMQVADGEKIFMVLVNAVFHPIPAGILLAAILAAIMSTADSQLLVSSSALAEDFYK
HHHCCCEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QLLRPQASSKEVLMVGRIGVILLALLALYFAMDPDSSVLSLVSYAWAGFGAAFGPVLILS
HHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IYWKRMNGKGALAGLLVGGITIVVWKQLTGGIFEVYEIVPGIIFASAAIVITSLVTAPPE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
KAVTDKFEEFERNLKTFK
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]