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The map label for this gene is rep [H]

Identifier: 119947075

GI number: 119947075

Start: 4293654

End: 4295663

Strand: Reverse

Name: rep [H]

Synonym: Ping_3473

Alternate gene names: 119947075

Gene position: 4295663-4293654 (Counterclockwise)

Preceding gene: 119947079

Following gene: 119947074

Centisome position: 94.21

GC content: 44.13

Gene sequence:

>2010_bases
ATGAAGCTTAATCCCGGACAAGATGCCGCCGTTAAAATGATCTCAGGCCCCTGCCTTGTTTTAGCGGGCGCGGGTAGCGG
TAAAACGCGTGTGATCACCAATAAAATTGCTCACCTGATCCAAAATTGTGATTATCTGGCCAAAAATATTGCCGCAGTGA
CCTTTACCAATAAAGCGGCTCGCGAGATGAAAGAGCGGGTTAGTCAAACGCTGGGTCGTAAAGAGGCGCGCGGTTTAATC
GTCTCAACCTTTCACTCATTAGGCTTGGATATTATCAAACGTGAAGTAAAAACGCTGGGCATGAAAACCGGTTTTACCTT
GTTCGATGGTCAGGATACTCTGGCATTATTAAAGGAGCTCACTGAAAAGCAGCTAAAAGAAGATAAGGATTTGCTTAAAG
CCTTAATGTCGCAAATTTCCAATTGGAAAAATGCCTTAGTATTACCCCCGCAGGCGATAAAGCAGGCGAAAGGTGAACGG
GAGGTCATATTTGCCCATTGTTATGACCTTTATCAGCGCCAGTTAAAAGCCTATAACGCCTTGGATTTTGACGATTTGAT
CGTCTTGCCTACGCTGTTGTTAACGCACAAAGAAGAAGTGCGTTTACGCTGGCAGAAAAGAATTCGCTATCTGCTGGTGG
ATGAATATCAGGATACTAATACCAGCCAGTATGAATTAATTAAAGCCTTAGTGGGAGAGCGTGCTCGCTTTACCGTAGTG
GGTGATGATGACCAGTCTATCTATTCATGGCGTGGTGCTCGTCCGGAAAATTTACTGCTGTTACAAAAAGATTTTCCGCA
GTTAAAAGTGGTAAAACTCGAGCAGAATTATCGCTCCAGTCAGCGTGTATTAAAATCAGCTAATGTCTTAATTGCCAATA
ACCCGCATGAATTTGAAAAACGTTTATTCAGTGAATTAGGCTATGGTGATGCCTTAAAAGTCCTGTTCGCAAATAATGAA
CAACATGAGGCGGAGCGGGTGGTGATGGAGCTTTCTGGACATAAGTTTATGAATGGGTGCTCCTTTAAAGATTATGCGGT
GCTCTATCGGGGCAATCATCAAAGCCGTTTATTAGAAAAGGTCATGATGGCAAACCGCATTCCCTATCGTATTAGCGGGG
GAACCTCTTTTTTTGCGCGCAGTGAAGTGAAAGACATTATGGCTTACCTTAAATTATTGGTGAATCATGAAGATGATAAT
GCTTTTTTGCGTATTGTGAATACACCGCGCCGCGGTATTGGCCCGACCTCTCTGGAAAAATTGGGCAGTTACGCCAATAT
GCGCCACAGTCGTTTGTTTACCTGCAGTTTTGAAATGGGTTTAGAGCAGACGTTAACTGGTAAAGGGTTACAGTCACTGC
GGGCTTTCACGCGCTGGTTGGTGGATCTTAATGACCGCCTTAAACGTGATCCGGCGATTGATGTTGTCCGTGATTTAATT
CGTGATATCGGTTATGAAGACTGGTTATATGAAAGCAGCCCCAGCCCTAAAGCCGCTGAAATGGGCATGAAAAATGTGTC
GACATTATTTAAATGGGTTACGGAAATGCTTAAAGGGGATGATCTTGATGAGGAAATGACTCTGGAGCAGGTTGTTGCCC
GTTTAACACTGCGCGATATGCTGTCGCGTAATGAAGCTGAGGAGGAGTTAGATCAGGTGCAGTTAATGACCCTGCATGCA
TCCAAAGGCCTGGAGTTTCCCTATGTGTATATGATTGGCATGGAGGAGGGGATTTTGCCGCATCAAATCAGTATTGAGGA
AGACAATGTTGAAGAAGAAAGACGCCTGACTTATGTAGGAATAACCCGGGCAAAAAAAGAGTTAACTTTCACTCTAGCCA
AAGAGCGGACTCAATATGGAGAAACCATTAAACCCACACCAAGCCGTTTTTTATTTGAATTACCGCAGGATGATTTAGAT
TGGGAAAGTGGGCCATCGAAAAAAACAGATCAGCAGAAAAAGCAGCTGGCGGACAAAGGGATTGCCAGTTTGCGTGCTCT
ATTCGATTAA

Upstream 100 bases:

>100_bases
ATGAAGAGGATCAGAAACTATATTGTTGATTGGTATTCAACTCAAACTGACGTAAAATCAGCAGCCGTTTACCCCAGTTA
TTTTGCAGGTAGGAAAAACC

Downstream 100 bases:

>100_bases
GCTCTGTCTTTCGGGATAGTGCCGGCGACTATTCGAAAGTGGCGGTTAGCGGTTTTGGAATCTCACCTTTAAGCCTTCGA
GCATAAAAATAACAAGTGCC

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 669; Mature: 669

Protein sequence:

>669_residues
MKLNPGQDAAVKMISGPCLVLAGAGSGKTRVITNKIAHLIQNCDYLAKNIAAVTFTNKAAREMKERVSQTLGRKEARGLI
VSTFHSLGLDIIKREVKTLGMKTGFTLFDGQDTLALLKELTEKQLKEDKDLLKALMSQISNWKNALVLPPQAIKQAKGER
EVIFAHCYDLYQRQLKAYNALDFDDLIVLPTLLLTHKEEVRLRWQKRIRYLLVDEYQDTNTSQYELIKALVGERARFTVV
GDDDQSIYSWRGARPENLLLLQKDFPQLKVVKLEQNYRSSQRVLKSANVLIANNPHEFEKRLFSELGYGDALKVLFANNE
QHEAERVVMELSGHKFMNGCSFKDYAVLYRGNHQSRLLEKVMMANRIPYRISGGTSFFARSEVKDIMAYLKLLVNHEDDN
AFLRIVNTPRRGIGPTSLEKLGSYANMRHSRLFTCSFEMGLEQTLTGKGLQSLRAFTRWLVDLNDRLKRDPAIDVVRDLI
RDIGYEDWLYESSPSPKAAEMGMKNVSTLFKWVTEMLKGDDLDEEMTLEQVVARLTLRDMLSRNEAEEELDQVQLMTLHA
SKGLEFPYVYMIGMEEGILPHQISIEEDNVEEERRLTYVGITRAKKELTFTLAKERTQYGETIKPTPSRFLFELPQDDLD
WESGPSKKTDQQKKQLADKGIASLRALFD

Sequences:

>Translated_669_residues
MKLNPGQDAAVKMISGPCLVLAGAGSGKTRVITNKIAHLIQNCDYLAKNIAAVTFTNKAAREMKERVSQTLGRKEARGLI
VSTFHSLGLDIIKREVKTLGMKTGFTLFDGQDTLALLKELTEKQLKEDKDLLKALMSQISNWKNALVLPPQAIKQAKGER
EVIFAHCYDLYQRQLKAYNALDFDDLIVLPTLLLTHKEEVRLRWQKRIRYLLVDEYQDTNTSQYELIKALVGERARFTVV
GDDDQSIYSWRGARPENLLLLQKDFPQLKVVKLEQNYRSSQRVLKSANVLIANNPHEFEKRLFSELGYGDALKVLFANNE
QHEAERVVMELSGHKFMNGCSFKDYAVLYRGNHQSRLLEKVMMANRIPYRISGGTSFFARSEVKDIMAYLKLLVNHEDDN
AFLRIVNTPRRGIGPTSLEKLGSYANMRHSRLFTCSFEMGLEQTLTGKGLQSLRAFTRWLVDLNDRLKRDPAIDVVRDLI
RDIGYEDWLYESSPSPKAAEMGMKNVSTLFKWVTEMLKGDDLDEEMTLEQVVARLTLRDMLSRNEAEEELDQVQLMTLHA
SKGLEFPYVYMIGMEEGILPHQISIEEDNVEEERRLTYVGITRAKKELTFTLAKERTQYGETIKPTPSRFLFELPQDDLD
WESGPSKKTDQQKKQLADKGIASLRALFD
>Mature_669_residues
MKLNPGQDAAVKMISGPCLVLAGAGSGKTRVITNKIAHLIQNCDYLAKNIAAVTFTNKAAREMKERVSQTLGRKEARGLI
VSTFHSLGLDIIKREVKTLGMKTGFTLFDGQDTLALLKELTEKQLKEDKDLLKALMSQISNWKNALVLPPQAIKQAKGER
EVIFAHCYDLYQRQLKAYNALDFDDLIVLPTLLLTHKEEVRLRWQKRIRYLLVDEYQDTNTSQYELIKALVGERARFTVV
GDDDQSIYSWRGARPENLLLLQKDFPQLKVVKLEQNYRSSQRVLKSANVLIANNPHEFEKRLFSELGYGDALKVLFANNE
QHEAERVVMELSGHKFMNGCSFKDYAVLYRGNHQSRLLEKVMMANRIPYRISGGTSFFARSEVKDIMAYLKLLVNHEDDN
AFLRIVNTPRRGIGPTSLEKLGSYANMRHSRLFTCSFEMGLEQTLTGKGLQSLRAFTRWLVDLNDRLKRDPAIDVVRDLI
RDIGYEDWLYESSPSPKAAEMGMKNVSTLFKWVTEMLKGDDLDEEMTLEQVVARLTLRDMLSRNEAEEELDQVQLMTLHA
SKGLEFPYVYMIGMEEGILPHQISIEEDNVEEERRLTYVGITRAKKELTFTLAKERTQYGETIKPTPSRFLFELPQDDLD
WESGPSKKTDQQKKQLADKGIASLRALFD

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=668, Percent_Identity=69.9101796407186, Blast_Score=961, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=640, Percent_Identity=37.8125, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1787196, Length=375, Percent_Identity=27.7333333333333, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=440, Percent_Identity=31.3636363636364, Blast_Score=194, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76597; Mature: 76597

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNPGQDAAVKMISGPCLVLAGAGSGKTRVITNKIAHLIQNCDYLAKNIAAVTFTNKAA
CCCCCCCCHHHHEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCHHH
REMKERVSQTLGRKEARGLIVSTFHSLGLDIIKREVKTLGMKTGFTLFDGQDTLALLKEL
HHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHH
TEKQLKEDKDLLKALMSQISNWKNALVLPPQAIKQAKGEREVIFAHCYDLYQRQLKAYNA
HHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
LDFDDLIVLPTLLLTHKEEVRLRWQKRIRYLLVDEYQDTNTSQYELIKALVGERARFTVV
CCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCCCCEEEEE
GDDDQSIYSWRGARPENLLLLQKDFPQLKVVKLEQNYRSSQRVLKSANVLIANNPHEFEK
CCCCCHHHHCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHCCEEEECCCHHHHH
RLFSELGYGDALKVLFANNEQHEAERVVMELSGHKFMNGCSFKDYAVLYRGNHQSRLLEK
HHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHCCCCCCCEEEEEECCCHHHHHHH
VMMANRIPYRISGGTSFFARSEVKDIMAYLKLLVNHEDDNAFLRIVNTPRRGIGPTSLEK
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHH
LGSYANMRHSRLFTCSFEMGLEQTLTGKGLQSLRAFTRWLVDLNDRLKRDPAIDVVRDLI
HHHHHCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RDIGYEDWLYESSPSPKAAEMGMKNVSTLFKWVTEMLKGDDLDEEMTLEQVVARLTLRDM
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LSRNEAEEELDQVQLMTLHASKGLEFPYVYMIGMEEGILPHQISIEEDNVEEERRLTYVG
HHCCHHHHHHHHHHHHEEECCCCCCCCEEEEEECCCCCCCCEEECCCCCCHHHHCEEEEE
ITRAKKELTFTLAKERTQYGETIKPTPSRFLFELPQDDLDWESGPSKKTDQQKKQLADKG
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHH
IASLRALFD
HHHHHHHCC
>Mature Secondary Structure
MKLNPGQDAAVKMISGPCLVLAGAGSGKTRVITNKIAHLIQNCDYLAKNIAAVTFTNKAA
CCCCCCCCHHHHEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCHHH
REMKERVSQTLGRKEARGLIVSTFHSLGLDIIKREVKTLGMKTGFTLFDGQDTLALLKEL
HHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHH
TEKQLKEDKDLLKALMSQISNWKNALVLPPQAIKQAKGEREVIFAHCYDLYQRQLKAYNA
HHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
LDFDDLIVLPTLLLTHKEEVRLRWQKRIRYLLVDEYQDTNTSQYELIKALVGERARFTVV
CCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCCCCEEEEE
GDDDQSIYSWRGARPENLLLLQKDFPQLKVVKLEQNYRSSQRVLKSANVLIANNPHEFEK
CCCCCHHHHCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHCCEEEECCCHHHHH
RLFSELGYGDALKVLFANNEQHEAERVVMELSGHKFMNGCSFKDYAVLYRGNHQSRLLEK
HHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHCCCCCCCEEEEEECCCHHHHHHH
VMMANRIPYRISGGTSFFARSEVKDIMAYLKLLVNHEDDNAFLRIVNTPRRGIGPTSLEK
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHH
LGSYANMRHSRLFTCSFEMGLEQTLTGKGLQSLRAFTRWLVDLNDRLKRDPAIDVVRDLI
HHHHHCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RDIGYEDWLYESSPSPKAAEMGMKNVSTLFKWVTEMLKGDDLDEEMTLEQVVARLTLRDM
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LSRNEAEEELDQVQLMTLHASKGLEFPYVYMIGMEEGILPHQISIEEDNVEEERRLTYVG
HHCCHHHHHHHHHHHHEEECCCCCCCCEEEEEECCCCCCCCEEECCCCCCHHHHCEEEEE
ITRAKKELTFTLAKERTQYGETIKPTPSRFLFELPQDDLDWESGPSKKTDQQKKQLADKG
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHH
IASLRALFD
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]