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The map label for this gene is rfbD [C]

Identifier: 119947065

GI number: 119947065

Start: 4283869

End: 4284753

Strand: Reverse

Name: rfbD [C]

Synonym: Ping_3463

Alternate gene names: 119947065

Gene position: 4284753-4283869 (Counterclockwise)

Preceding gene: 119947066

Following gene: 119947064

Centisome position: 93.97

GC content: 41.24

Gene sequence:

>885_bases
ATGCGCGTATTAATAACCGGAAGTAAAGGACAGGTTGGTCATTGCCTAACGGAACAGTTAAGAAATTATACTGATGCACA
GTTTTTAGCGTTAGATTCAAAAGGCTTAGACATCACTGATCAATCTGCTGTCCAAGCAGTAGTGAATGAGTTTAAACCAA
CCATCATCATTAATGCTGCCGCATACACCGCAGTTGACCGAGCCGAAGATGAGTCTGATCTTGCTTACGCTATTAATCGT
GATGGTGCAAAGTATTTAGCAGAAGCTGCACAAAGTGTAAATGCAGTGATTTTACATATCTCAACGGATTATGTATTTTC
CGGCGATAAAGAGGGTATGTATACCGAGGCCGATCTAACCTCACCACAGGGAATATACGGTGAAAGTAAATTAGCCGGTG
AGAAAGCCGTTATTGAGGCGTGTTCCCGGCATATTATTCTGCGAACTGCATGGGTATTTGGTGAGCATGGTAATAACTTT
GTCAAAATCATGCTGCGTCTAGCCAAAACCCACGATACCTTAGGCATTGTGGGGGATCAATTTGGTGGTCCAACTTATGC
AGGTGATATCGCATCGGCATTGGTTACTGTTGCAAAAACGCTCAGTAATGGTGATAACGATAAATATGGTATTTATCATT
ATTCTGGTTTCCCTCATGTCAGCTGGAGCCAGTTTGCTCAAGCTATTTTTGATGAGGCTGTTCAACAAACGGTACTAAAA
CAGTCACCTGTTGTTAACTCAATTACAACGGCAGATTACCCAACTCCCGCTAAGCGGCCAGCTAATTCAAAATTATCTAC
AACATTAATCAAAGACAAATTTGGGGTTAATGCAAGTGATTGGAAGGCTGCGCTACAGAATATTAAAGCGTATACAAATA
TATAA

Upstream 100 bases:

>100_bases
GATGAACAGGTTCTTGCACTTGCTAATCCTATGATGAAAAACGAATATGGGCAATACCTAAAACGTTTAATTGAAGAATC
AAAAGCGAAGGCATAAAGAT

Downstream 100 bases:

>100_bases
CAGATTGAGTTTATTATGAAAATTATTGAAACAAAAATTCCTGATGTGAAAATCATTGAACCAGCCGTGTTTGGTGATGA
ACGAGGTTTCTTTATGGAAA

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MRVLITGSKGQVGHCLTEQLRNYTDAQFLALDSKGLDITDQSAVQAVVNEFKPTIIINAAAYTAVDRAEDESDLAYAINR
DGAKYLAEAAQSVNAVILHISTDYVFSGDKEGMYTEADLTSPQGIYGESKLAGEKAVIEACSRHIILRTAWVFGEHGNNF
VKIMLRLAKTHDTLGIVGDQFGGPTYAGDIASALVTVAKTLSNGDNDKYGIYHYSGFPHVSWSQFAQAIFDEAVQQTVLK
QSPVVNSITTADYPTPAKRPANSKLSTTLIKDKFGVNASDWKAALQNIKAYTNI

Sequences:

>Translated_294_residues
MRVLITGSKGQVGHCLTEQLRNYTDAQFLALDSKGLDITDQSAVQAVVNEFKPTIIINAAAYTAVDRAEDESDLAYAINR
DGAKYLAEAAQSVNAVILHISTDYVFSGDKEGMYTEADLTSPQGIYGESKLAGEKAVIEACSRHIILRTAWVFGEHGNNF
VKIMLRLAKTHDTLGIVGDQFGGPTYAGDIASALVTVAKTLSNGDNDKYGIYHYSGFPHVSWSQFAQAIFDEAVQQTVLK
QSPVVNSITTADYPTPAKRPANSKLSTTLIKDKFGVNASDWKAALQNIKAYTNI
>Mature_294_residues
MRVLITGSKGQVGHCLTEQLRNYTDAQFLALDSKGLDITDQSAVQAVVNEFKPTIIINAAAYTAVDRAEDESDLAYAINR
DGAKYLAEAAQSVNAVILHISTDYVFSGDKEGMYTEADLTSPQGIYGESKLAGEKAVIEACSRHIILRTAWVFGEHGNNF
VKIMLRLAKTHDTLGIVGDQFGGPTYAGDIASALVTVAKTLSNGDNDKYGIYHYSGFPHVSWSQFAQAIFDEAVQQTVLK
QSPVVNSITTADYPTPAKRPANSKLSTTLIKDKFGVNASDWKAALQNIKAYTNI

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=302, Percent_Identity=27.4834437086093, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI33519455, Length=302, Percent_Identity=27.4834437086093, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1788352, Length=292, Percent_Identity=38.3561643835616, Blast_Score=212, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 31863; Mature: 31863

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLITGSKGQVGHCLTEQLRNYTDAQFLALDSKGLDITDQSAVQAVVNEFKPTIIINAA
CEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEHH
AYTAVDRAEDESDLAYAINRDGAKYLAEAAQSVNAVILHISTDYVFSGDKEGMYTEADLT
HHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEEECEEECCCCCCCEEECCCC
SPQGIYGESKLAGEKAVIEACSRHIILRTAWVFGEHGNNFVKIMLRLAKTHDTLGIVGDQ
CCCCCCCCHHHCCHHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEECCCC
FGGPTYAGDIASALVTVAKTLSNGDNDKYGIYHYSGFPHVSWSQFAQAIFDEAVQQTVLK
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
QSPVVNSITTADYPTPAKRPANSKLSTTLIKDKFGVNASDWKAALQNIKAYTNI
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVLITGSKGQVGHCLTEQLRNYTDAQFLALDSKGLDITDQSAVQAVVNEFKPTIIINAA
CEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEHH
AYTAVDRAEDESDLAYAINRDGAKYLAEAAQSVNAVILHISTDYVFSGDKEGMYTEADLT
HHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEEECEEECCCCCCCEEECCCC
SPQGIYGESKLAGEKAVIEACSRHIILRTAWVFGEHGNNFVKIMLRLAKTHDTLGIVGDQ
CCCCCCCCHHHCCHHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEECCCC
FGGPTYAGDIASALVTVAKTLSNGDNDKYGIYHYSGFPHVSWSQFAQAIFDEAVQQTVLK
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
QSPVVNSITTADYPTPAKRPANSKLSTTLIKDKFGVNASDWKAALQNIKAYTNI
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]