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The map label for this gene is opuAB [H]

Identifier: 119947028

GI number: 119947028

Start: 4232406

End: 4234466

Strand: Reverse

Name: opuAB [H]

Synonym: Ping_3425

Alternate gene names: 119947028

Gene position: 4234466-4232406 (Counterclockwise)

Preceding gene: 119947029

Following gene: 119947027

Centisome position: 92.87

GC content: 47.74

Gene sequence:

>2061_bases
ATGCAATATTTTACCGGACATAAATGGACTCAGTTTGTCGCTTTATTGGCGGTGTTCTTTGCCCTTGTAATCGTCATCGA
CCCCGCCACTGGTGGTAACTTATGGCGCCTACAAGAGACCAGTTTCTGGCTGTTGCCTGACATTGTTAACGACGGTCTTA
AGTTCCTTATTGATGACTTTTGGGTCGTAGAGATTTACGATCCTGAGTTAGACATCACCGAAGAATCGACCATGATACAA
GAGTTTACGCGCGGCCTGTCTGGCGTGATTCTCTTTAGCATTAATTTGATTCGAGAACTGCTATTAGGTGGCGTTAAAAC
CGTGGTGGCTTTTACCAGCTGGGAATTTATCAGCGCAAACGAATGGGCTCGCATCCCAGCACTGCCATGGCCAGCGGTGG
CTGCGGGTGCCTTTTTAATTGGCCACTATCTTGGGGGCCGTAGCCTGTCTATTTTAGCCGGCACCAGCACCCTTTACATT
GCTGTATTTGGTCAGTGGACACCCGCCATGCAAACCCTTTCCTTTGTGTTAGTAGCGGTGCCCGTGTCGGTTTTCCTGGG
ATTAACCTCAGGTATACTGGCCTTTAAGAACAAAAGCTTCGAGTCCATCCTGACGCCACTGTTAAACGTGGCTCAATCCA
TGCCTCACTTCTCTTACTTAATCCCAGTGGTAGTATTCTTTGGCATTGGTGACCATGCGGGTGCTATTTCAACAATTATT
TTCGCCACTCCCCCAATGATCCGCTTAACTATTTTAGGCCTTAAAAAAGTCTCTCCGGAGGTGTTGGAGGCTGGCATGAT
GAGTGGCTGCAACGCACGCCAACTGATGTTCAGGGTGCTCATTCCAACAGCACGCCATGACATTCTGATCGGCGTTAACC
AGGTTATTATGCAGTGTTTGGCCATGGCCGTCATCGCCTCTTTTATTGGTGCAAAGGGGTTGGGCTACGACTTACTGGTG
TCATTGAACATGCTCAATATAGGCCAGGCCCTCGAACTGGGTGTGTCTATTGTATTGATTGCTGTGGTTTTGGATAAGTT
ATCCCTGGCCTGGGCTAACAAGCAGAGCGATTACTTTGCTGAATTACCCTTCCATTTGCGTCACAAGCATTCGCTCGCCT
TCTTGGGAATTTTGATCGTCACCTCTGCTATTGCCTACGCTGTGACGGTGGTTTTCCCAGACAAGGCTAACTACCTCTAC
TTCATCAATGATTCACAAGGGTTGACTACCGAAAAGTTCTGGGACTTTATCGTTGACTGGATCATCACCAACACTTACGA
CGCCGTGCAAAGCTTCAACTTCTTCATGATCACCTCAATTTTGATGCCCATGAAAGAGGCTTATCTATCGATGCCAGTCG
CTGCAACCCTCTTTCTAGTTGCAGGCACCGGTTACATTGTTGGAGGCATTCGCTCAGCTATTATTGTGACAGCCTTGATT
GCCTTTATTGCGTTAACACCCTGGTGGGACCAAGCCCTCATTACAGCCTATATGACCACCTTCGCGGTCATCATTTCTGT
TACTATTGGTTGCAGTGTTGGCATTTTGTGCTCTCGAACCCCCCGAGCAACTAAAGTTATATTGCTGGTTTGTGACACCT
TCCAGACATTCCCGTCGTTCGTCTACTTAATTCCCGTCATCATGTTGTTTGGCGTGAGTGATTTATCAGTGCTTATTGCG
GTGATCGTATACGCTACTATCCCTGCCACACGTTACACCGTAGAAGGGTTGAGTAACGTGCCTAAATCCTTGCAAGAAGC
AGGCTCCATGTCAGGGGTTAACCGCATGCAGCGTTTATTAAAAATTGAACTACCTTTGGCGTTCCCACACATCATGTTGG
GCATTAACCAAACCGTTATCTTCGCTTTGTTTATGGTTATTATCGGCGCCTTTATCGGTACTGACGACTTAGGTCAGCTG
ATCATGCAATCCTTGTCTCAAGCCAACGCTATGGGCCAAGGCATTGTGCTCGGCTTATGCGTCGCCTTTATCGGCTTGGC
AGTAGACCATTTGATCCATACTTGGGCAGAGCAGCGTAAGAAAGTGTTAGGGATTGCGTAG

Upstream 100 bases:

>100_bases
GTAATATTGTTGGTGTGTTAAACCGCAACATCATTATGGACGTCTTGTTTGATGAAGCTAACAGCATGCCAGTGAATACA
TCAGATGAGAGCCAAACCTA

Downstream 100 bases:

>100_bases
CCGAATTGGTTGGATTATTCGTAAAAAGGTATGGGTGTCCAATCTGAACTATTGTTATTTCCGCATATGCGTCCACAAAC
AAAATAATGGTTTGAGGCTT

Product: ABC transporter substrate binding inner membrane protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MQYFTGHKWTQFVALLAVFFALVIVIDPATGGNLWRLQETSFWLLPDIVNDGLKFLIDDFWVVEIYDPELDITEESTMIQ
EFTRGLSGVILFSINLIRELLLGGVKTVVAFTSWEFISANEWARIPALPWPAVAAGAFLIGHYLGGRSLSILAGTSTLYI
AVFGQWTPAMQTLSFVLVAVPVSVFLGLTSGILAFKNKSFESILTPLLNVAQSMPHFSYLIPVVVFFGIGDHAGAISTII
FATPPMIRLTILGLKKVSPEVLEAGMMSGCNARQLMFRVLIPTARHDILIGVNQVIMQCLAMAVIASFIGAKGLGYDLLV
SLNMLNIGQALELGVSIVLIAVVLDKLSLAWANKQSDYFAELPFHLRHKHSLAFLGILIVTSAIAYAVTVVFPDKANYLY
FINDSQGLTTEKFWDFIVDWIITNTYDAVQSFNFFMITSILMPMKEAYLSMPVAATLFLVAGTGYIVGGIRSAIIVTALI
AFIALTPWWDQALITAYMTTFAVIISVTIGCSVGILCSRTPRATKVILLVCDTFQTFPSFVYLIPVIMLFGVSDLSVLIA
VIVYATIPATRYTVEGLSNVPKSLQEAGSMSGVNRMQRLLKIELPLAFPHIMLGINQTVIFALFMVIIGAFIGTDDLGQL
IMQSLSQANAMGQGIVLGLCVAFIGLAVDHLIHTWAEQRKKVLGIA

Sequences:

>Translated_686_residues
MQYFTGHKWTQFVALLAVFFALVIVIDPATGGNLWRLQETSFWLLPDIVNDGLKFLIDDFWVVEIYDPELDITEESTMIQ
EFTRGLSGVILFSINLIRELLLGGVKTVVAFTSWEFISANEWARIPALPWPAVAAGAFLIGHYLGGRSLSILAGTSTLYI
AVFGQWTPAMQTLSFVLVAVPVSVFLGLTSGILAFKNKSFESILTPLLNVAQSMPHFSYLIPVVVFFGIGDHAGAISTII
FATPPMIRLTILGLKKVSPEVLEAGMMSGCNARQLMFRVLIPTARHDILIGVNQVIMQCLAMAVIASFIGAKGLGYDLLV
SLNMLNIGQALELGVSIVLIAVVLDKLSLAWANKQSDYFAELPFHLRHKHSLAFLGILIVTSAIAYAVTVVFPDKANYLY
FINDSQGLTTEKFWDFIVDWIITNTYDAVQSFNFFMITSILMPMKEAYLSMPVAATLFLVAGTGYIVGGIRSAIIVTALI
AFIALTPWWDQALITAYMTTFAVIISVTIGCSVGILCSRTPRATKVILLVCDTFQTFPSFVYLIPVIMLFGVSDLSVLIA
VIVYATIPATRYTVEGLSNVPKSLQEAGSMSGVNRMQRLLKIELPLAFPHIMLGINQTVIFALFMVIIGAFIGTDDLGQL
IMQSLSQANAMGQGIVLGLCVAFIGLAVDHLIHTWAEQRKKVLGIA
>Mature_686_residues
MQYFTGHKWTQFVALLAVFFALVIVIDPATGGNLWRLQETSFWLLPDIVNDGLKFLIDDFWVVEIYDPELDITEESTMIQ
EFTRGLSGVILFSINLIRELLLGGVKTVVAFTSWEFISANEWARIPALPWPAVAAGAFLIGHYLGGRSLSILAGTSTLYI
AVFGQWTPAMQTLSFVLVAVPVSVFLGLTSGILAFKNKSFESILTPLLNVAQSMPHFSYLIPVVVFFGIGDHAGAISTII
FATPPMIRLTILGLKKVSPEVLEAGMMSGCNARQLMFRVLIPTARHDILIGVNQVIMQCLAMAVIASFIGAKGLGYDLLV
SLNMLNIGQALELGVSIVLIAVVLDKLSLAWANKQSDYFAELPFHLRHKHSLAFLGILIVTSAIAYAVTVVFPDKANYLY
FINDSQGLTTEKFWDFIVDWIITNTYDAVQSFNFFMITSILMPMKEAYLSMPVAATLFLVAGTGYIVGGIRSAIIVTALI
AFIALTPWWDQALITAYMTTFAVIISVTIGCSVGILCSRTPRATKVILLVCDTFQTFPSFVYLIPVIMLFGVSDLSVLIA
VIVYATIPATRYTVEGLSNVPKSLQEAGSMSGVNRMQRLLKIELPLAFPHIMLGINQTVIFALFMVIIGAFIGTDDLGQL
IMQSLSQANAMGQGIVLGLCVAFIGLAVDHLIHTWAEQRKKVLGIA

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=212, Percent_Identity=41.0377358490566, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788449, Length=146, Percent_Identity=31.5068493150685, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788451, Length=173, Percent_Identity=28.3236994219653, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 75015; Mature: 75015

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYFTGHKWTQFVALLAVFFALVIVIDPATGGNLWRLQETSFWLLPDIVNDGLKFLIDDF
CCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCEEEEECCCEEECHHHHHHHHHHHHCCE
WVVEIYDPELDITEESTMIQEFTRGLSGVILFSINLIRELLLGGVKTVVAFTSWEFISAN
EEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEECCC
EWARIPALPWPAVAAGAFLIGHYLGGRSLSILAGTSTLYIAVFGQWTPAMQTLSFVLVAV
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHH
PVSVFLGLTSGILAFKNKSFESILTPLLNVAQSMPHFSYLIPVVVFFGIGDHAGAISTII
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHH
FATPPMIRLTILGLKKVSPEVLEAGMMSGCNARQLMFRVLIPTARHDILIGVNQVIMQCL
CCCCCHHEEEEHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHEECHHHHHHHHH
AMAVIASFIGAKGLGYDLLVSLNMLNIGQALELGVSIVLIAVVLDKLSLAWANKQSDYFA
HHHHHHHHHCCCCCCHHHHHEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
ELPFHLRHKHSLAFLGILIVTSAIAYAVTVVFPDKANYLYFINDSQGLTTEKFWDFIVDW
HCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHH
IITNTYDAVQSFNFFMITSILMPMKEAYLSMPVAATLFLVAGTGYIVGGIRSAIIVTALI
HHHCCHHHHHHCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AFIALTPWWDQALITAYMTTFAVIISVTIGCSVGILCSRTPRATKVILLVCDTFQTFPSF
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEEEHHHHHHHHH
VYLIPVIMLFGVSDLSVLIAVIVYATIPATRYTVEGLSNVPKSLQEAGSMSGVNRMQRLL
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHH
KIELPLAFPHIMLGINQTVIFALFMVIIGAFIGTDDLGQLIMQSLSQANAMGQGIVLGLC
HHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHH
VAFIGLAVDHLIHTWAEQRKKVLGIA
HHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQYFTGHKWTQFVALLAVFFALVIVIDPATGGNLWRLQETSFWLLPDIVNDGLKFLIDDF
CCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCEEEEECCCEEECHHHHHHHHHHHHCCE
WVVEIYDPELDITEESTMIQEFTRGLSGVILFSINLIRELLLGGVKTVVAFTSWEFISAN
EEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEECCC
EWARIPALPWPAVAAGAFLIGHYLGGRSLSILAGTSTLYIAVFGQWTPAMQTLSFVLVAV
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHH
PVSVFLGLTSGILAFKNKSFESILTPLLNVAQSMPHFSYLIPVVVFFGIGDHAGAISTII
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHH
FATPPMIRLTILGLKKVSPEVLEAGMMSGCNARQLMFRVLIPTARHDILIGVNQVIMQCL
CCCCCHHEEEEHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHEECHHHHHHHHH
AMAVIASFIGAKGLGYDLLVSLNMLNIGQALELGVSIVLIAVVLDKLSLAWANKQSDYFA
HHHHHHHHHCCCCCCHHHHHEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
ELPFHLRHKHSLAFLGILIVTSAIAYAVTVVFPDKANYLYFINDSQGLTTEKFWDFIVDW
HCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHH
IITNTYDAVQSFNFFMITSILMPMKEAYLSMPVAATLFLVAGTGYIVGGIRSAIIVTALI
HHHCCHHHHHHCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AFIALTPWWDQALITAYMTTFAVIISVTIGCSVGILCSRTPRATKVILLVCDTFQTFPSF
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEEEHHHHHHHHH
VYLIPVIMLFGVSDLSVLIAVIVYATIPATRYTVEGLSNVPKSLQEAGSMSGVNRMQRLL
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHH
KIELPLAFPHIMLGINQTVIFALFMVIIGAFIGTDDLGQLIMQSLSQANAMGQGIVLGLC
HHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHH
VAFIGLAVDHLIHTWAEQRKKVLGIA
HHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]