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The map label for this gene is gshA [H]

Identifier: 119946978

GI number: 119946978

Start: 4165579

End: 4167159

Strand: Reverse

Name: gshA [H]

Synonym: Ping_3373

Alternate gene names: 119946978

Gene position: 4167159-4165579 (Counterclockwise)

Preceding gene: 119946979

Following gene: 119946977

Centisome position: 91.39

GC content: 39.15

Gene sequence:

>1581_bases
TTGTCTCTCAATTTTAATCAGCGTTTAGCTGTTTTTGAACAACAATCACAATCTCTTGAAGGTTTTGGCCGTGGTATTGA
ACGCGAAGCTTTACGCGTGACGCCCGATGGGGGCTTATCGCAAAATAGCCATCCCTATGCTTTTGGCAGCGCTTTATGTC
ACAGTACGATCACCACTGATTATGCTGAAGGCCTATTAGAGTTTATCACCCCCGTCGCTAAAGATGTCGATCAGCTATTT
GCCTATTTAAACGATGTTCACCATTACGCGGCTAAAAATTTAGAAAATAATGAAACATTATGGCCTATCAGTATGCCCTG
TTTTATTAATAATGAAGACTCAGTTGAACTTGCCCAGTTCGGTTCATCTAATGTTGGAAAAATGAAAACCGCCTATCGTC
AAGGGTTAAAAAATCGTTATGGCAGTATGATGCAGGTCATCGCTGGTGTGCATTATAACTTTTCATTTCCGGATGGTTTT
TGGGACGCCTGGGCTAAAGCCAATCAATCACCTTTAACGGGAAAAGAGGCTCAGTCTGCAGGTTATATGGGGTTAATCAG
GAATTACTTGCGTTTTGGCTGGGTTATTCCTTATCTGTTTGGGGCATCTCCGGCACTTTGTAAATCCTTTTTACAGGGTA
AAAAAACACACTTCAAATTTAAAGAAACGGGCTCAGGAACAATTTATTTACCTTATGCAACGTCGTTGCGTATGAGTGAT
TTAGGTTATACAAGCAGTGCTCAATCTTCACTTGATATTTGTTATAACAGTCTTGATAATTATATCGGCTCAGTGCGCAA
AGCTTTAACGCAAAAAAATTCAGACTTTGTTCATTTAGGTATTAAAGAAAATGATGAATATATTCAACTAAATGACAATG
TACTACAAATTGAAAACGAGCTTTATGCTTCGATTCGTGCAAAACGGGTGCAGAAAGAGGGTGAAACACCTTTGCAGGCA
TTAGATGCGCGCGGTATTGAGTATGTTGAAATCCGTTCTTTAGATGTAAATCCATTTTCTGAAGTAGGTATCACAGAAGA
GCAGGTAAATTTCTTGGATCTGTTTCTTACCTGGTGTGCGATGACTCCCTCTGAGAGTATCTCTAAATTACAATCGCAGC
ACTTTTCAGAAAACTTAAATAAAGTGGTGACAGAAGGCCGCCGGCCTAATTTAATGCTGGAAATCGATGGAAAAGAGCAA
AATGTAGCGGCTTGGGGGGATTGGTTAACGGGGCAGTTAAAAGAACTTGCTGTTGTCATGGATAAAGAAAGTGCTTCAGA
TCGTTATCAGAATGCCATTGCCCACATTGCGCCTCGCTTTGCTTCGCCAGAATTAACCAGTTCTGCGCGAATTTTAGAAA
CCCTTAAAGAGGGGGATTTAGATAACGGCCGGTTAGCATTGAATTTATCGAAAACTTATAAAGAAAAGTTAATAAAGCAG
AACTATCAGGTTTGGTCTGATGCTTTCTTTGAAAATGAAAAAATAATTTCTGAGAAAAAACAGAAAGAAATAGAAGAAAG
TGACAATCTGGATTTTGATGGATACCTGCAGAACTATTTTTTAAATGCGCAAAAAAAATAG

Upstream 100 bases:

>100_bases
TTTATATGTTGTTCGGCGCTTATTATTTTTTTGAACTTGTAAATGGTATCTTTATTAAGGTACTCTGCTGAGACTTTTCG
TTATTTCAAAGGGTAAGCTC

Downstream 100 bases:

>100_bases
TAACGATGGGATTATAAAAACTTAAGTTATATGCCCAAATGACTGAATATGCAGGAATCTGGCTCTTCAAGTCATTTGGG
TGTAGCGATTTATAAAGCTG

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSLNFNQRLAVFEQQSQSLEGFGRGIEREALRVTPDGGLSQNSHPYAFGSALCHSTITTDYAEGLLEFITPVAKDVDQLF
AYLNDVHHYAAKNLENNETLWPISMPCFINNEDSVELAQFGSSNVGKMKTAYRQGLKNRYGSMMQVIAGVHYNFSFPDGF
WDAWAKANQSPLTGKEAQSAGYMGLIRNYLRFGWVIPYLFGASPALCKSFLQGKKTHFKFKETGSGTIYLPYATSLRMSD
LGYTSSAQSSLDICYNSLDNYIGSVRKALTQKNSDFVHLGIKENDEYIQLNDNVLQIENELYASIRAKRVQKEGETPLQA
LDARGIEYVEIRSLDVNPFSEVGITEEQVNFLDLFLTWCAMTPSESISKLQSQHFSENLNKVVTEGRRPNLMLEIDGKEQ
NVAAWGDWLTGQLKELAVVMDKESASDRYQNAIAHIAPRFASPELTSSARILETLKEGDLDNGRLALNLSKTYKEKLIKQ
NYQVWSDAFFENEKIISEKKQKEIEESDNLDFDGYLQNYFLNAQKK

Sequences:

>Translated_526_residues
MSLNFNQRLAVFEQQSQSLEGFGRGIEREALRVTPDGGLSQNSHPYAFGSALCHSTITTDYAEGLLEFITPVAKDVDQLF
AYLNDVHHYAAKNLENNETLWPISMPCFINNEDSVELAQFGSSNVGKMKTAYRQGLKNRYGSMMQVIAGVHYNFSFPDGF
WDAWAKANQSPLTGKEAQSAGYMGLIRNYLRFGWVIPYLFGASPALCKSFLQGKKTHFKFKETGSGTIYLPYATSLRMSD
LGYTSSAQSSLDICYNSLDNYIGSVRKALTQKNSDFVHLGIKENDEYIQLNDNVLQIENELYASIRAKRVQKEGETPLQA
LDARGIEYVEIRSLDVNPFSEVGITEEQVNFLDLFLTWCAMTPSESISKLQSQHFSENLNKVVTEGRRPNLMLEIDGKEQ
NVAAWGDWLTGQLKELAVVMDKESASDRYQNAIAHIAPRFASPELTSSARILETLKEGDLDNGRLALNLSKTYKEKLIKQ
NYQVWSDAFFENEKIISEKKQKEIEESDNLDFDGYLQNYFLNAQKK
>Mature_525_residues
SLNFNQRLAVFEQQSQSLEGFGRGIEREALRVTPDGGLSQNSHPYAFGSALCHSTITTDYAEGLLEFITPVAKDVDQLFA
YLNDVHHYAAKNLENNETLWPISMPCFINNEDSVELAQFGSSNVGKMKTAYRQGLKNRYGSMMQVIAGVHYNFSFPDGFW
DAWAKANQSPLTGKEAQSAGYMGLIRNYLRFGWVIPYLFGASPALCKSFLQGKKTHFKFKETGSGTIYLPYATSLRMSDL
GYTSSAQSSLDICYNSLDNYIGSVRKALTQKNSDFVHLGIKENDEYIQLNDNVLQIENELYASIRAKRVQKEGETPLQAL
DARGIEYVEIRSLDVNPFSEVGITEEQVNFLDLFLTWCAMTPSESISKLQSQHFSENLNKVVTEGRRPNLMLEIDGKEQN
VAAWGDWLTGQLKELAVVMDKESASDRYQNAIAHIAPRFASPELTSSARILETLKEGDLDNGRLALNLSKTYKEKLIKQN
YQVWSDAFFENEKIISEKKQKEIEESDNLDFDGYLQNYFLNAQKK

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=521, Percent_Identity=46.0652591170825, Blast_Score=484, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 59452; Mature: 59321

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNFNQRLAVFEQQSQSLEGFGRGIEREALRVTPDGGLSQNSHPYAFGSALCHSTITTD
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
YAEGLLEFITPVAKDVDQLFAYLNDVHHYAAKNLENNETLWPISMPCFINNEDSVELAQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCHHHHC
GSSNVGKMKTAYRQGLKNRYGSMMQVIAGVHYNFSFPDGFWDAWAKANQSPLTGKEAQSA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHCC
GYMGLIRNYLRFGWVIPYLFGASPALCKSFLQGKKTHFKFKETGSGTIYLPYATSLRMSD
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCEEHHC
LGYTSSAQSSLDICYNSLDNYIGSVRKALTQKNSDFVHLGIKENDEYIQLNDNVLQIENE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCEEEHHHH
LYASIRAKRVQKEGETPLQALDARGIEYVEIRSLDVNPFSEVGITEEQVNFLDLFLTWCA
HHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHH
MTPSESISKLQSQHFSENLNKVVTEGRRPNLMLEIDGKEQNVAAWGDWLTGQLKELAVVM
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
DKESASDRYQNAIAHIAPRFASPELTSSARILETLKEGDLDNGRLALNLSKTYKEKLIKQ
CCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHH
NYQVWSDAFFENEKIISEKKQKEIEESDNLDFDGYLQNYFLNAQKK
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SLNFNQRLAVFEQQSQSLEGFGRGIEREALRVTPDGGLSQNSHPYAFGSALCHSTITTD
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
YAEGLLEFITPVAKDVDQLFAYLNDVHHYAAKNLENNETLWPISMPCFINNEDSVELAQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCHHHHC
GSSNVGKMKTAYRQGLKNRYGSMMQVIAGVHYNFSFPDGFWDAWAKANQSPLTGKEAQSA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHCC
GYMGLIRNYLRFGWVIPYLFGASPALCKSFLQGKKTHFKFKETGSGTIYLPYATSLRMSD
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCEEHHC
LGYTSSAQSSLDICYNSLDNYIGSVRKALTQKNSDFVHLGIKENDEYIQLNDNVLQIENE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCEEEHHHH
LYASIRAKRVQKEGETPLQALDARGIEYVEIRSLDVNPFSEVGITEEQVNFLDLFLTWCA
HHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHH
MTPSESISKLQSQHFSENLNKVVTEGRRPNLMLEIDGKEQNVAAWGDWLTGQLKELAVVM
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
DKESASDRYQNAIAHIAPRFASPELTSSARILETLKEGDLDNGRLALNLSKTYKEKLIKQ
CCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHH
NYQVWSDAFFENEKIISEKKQKEIEESDNLDFDGYLQNYFLNAQKK
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA