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The map label for this gene is yjeA [H]

Identifier: 119946883

GI number: 119946883

Start: 4043423

End: 4044403

Strand: Reverse

Name: yjeA [H]

Synonym: Ping_3277

Alternate gene names: 119946883

Gene position: 4044403-4043423 (Counterclockwise)

Preceding gene: 119946888

Following gene: 119946882

Centisome position: 88.7

GC content: 42.0

Gene sequence:

>981_bases
ATGAGCTGGAAACCAAGTGCATCGATAAAAGAATTAAAACAACGGGCAAAAATATTACAGGCTATTCGGCATTTTTTTAT
AACACGCGGATTATTAGAAGTGGAGACGCCGATCTTAAGCCAGGCGGGTGTGACGGATATCCACTTGTTTTGTTTTCAAA
CCCATTTTATGGGGCCACAAATATCCTCATCAAAGCCGGAAGCACAGGGTACCCCGCTCTATCTGCAAACGTCGCCTGAA
TTTCATATGAAGCGTCTTTTGTCTGCCGGTAGTGGGTGTATTTTTCAAATAAGTAAAGTCTTTCGCAATGAAGAATCGGG
ACGATTCCATAATCCTGAATTTACCTTACTGGAATGGTACCGGGTTGGATTTGACCATTTTCGTTTAATGGATGAAATGG
ATCAGTTATTACAATTTATTCTTAAATCTGAAACTGCGCGTCGTTGTACCTATCAGAATGCTTTTTTAGAAGTATTAGCG
GTTGATCCTCTCACTGCGAGTCTTGAACAATTGAAGACTTCAGGAGAAAAGCTTAATCTGGGGGAGGTCTTAAGTGAAGA
ACCCGATCGGGATACTGTACTGCAGCTATTATTTTGTATTGCTATTGAACCTGTTATTGCGCAACACAAGCCCTGTTTTA
TTTATAATTTTCCGGCTTCACAAGCAGCATTGGCAAGAATAAGTCCCACTGATAAGCGTGTCGCAGAACGTTTTGAGGTT
TATTTTCAAGGCATTGAGCTGGCTAATGGTTTTCATGAATTAAGTGACGGGCAAGAGCAGTTAAGACGTTTTCAGGCAGA
TAATCGTTTACGCTCACGAAAGGGGCTTGCGCAAATGCCGATTGATGGGAACTTTATCTCGTCGCTAGCGACGTTACCCG
ATTGTGCCGGTGTTGCCTTAGGAGTCGATCGCTTGGTAATGCTTGCCTGCGAGAAAAAACATATTGATGATGTTATCAGT
TTTACAATTAAAGGAGCCTGA

Upstream 100 bases:

>100_bases
TTTTATTCTTCGGTAAACTAGGGGGGGGTATTGATCTTCCTATGCGATAATCTTTGATTATCTAGAAAAGGTCAATTATT
CTGAATATAAAGGAATTAAA

Downstream 100 bases:

>100_bases
GTTGGCTAAATTATGGTTAACCGATCGTGCTTATCCGTTATGGGCGTATATATTTCGTATCTTATACTGGTTATTTGCTA
TTTATCTTTCCTCTTTATAT

Product: lysyl-tRNA synthetase-related protein GenX

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSWKPSASIKELKQRAKILQAIRHFFITRGLLEVETPILSQAGVTDIHLFCFQTHFMGPQISSSKPEAQGTPLYLQTSPE
FHMKRLLSAGSGCIFQISKVFRNEESGRFHNPEFTLLEWYRVGFDHFRLMDEMDQLLQFILKSETARRCTYQNAFLEVLA
VDPLTASLEQLKTSGEKLNLGEVLSEEPDRDTVLQLLFCIAIEPVIAQHKPCFIYNFPASQAALARISPTDKRVAERFEV
YFQGIELANGFHELSDGQEQLRRFQADNRLRSRKGLAQMPIDGNFISSLATLPDCAGVALGVDRLVMLACEKKHIDDVIS
FTIKGA

Sequences:

>Translated_326_residues
MSWKPSASIKELKQRAKILQAIRHFFITRGLLEVETPILSQAGVTDIHLFCFQTHFMGPQISSSKPEAQGTPLYLQTSPE
FHMKRLLSAGSGCIFQISKVFRNEESGRFHNPEFTLLEWYRVGFDHFRLMDEMDQLLQFILKSETARRCTYQNAFLEVLA
VDPLTASLEQLKTSGEKLNLGEVLSEEPDRDTVLQLLFCIAIEPVIAQHKPCFIYNFPASQAALARISPTDKRVAERFEV
YFQGIELANGFHELSDGQEQLRRFQADNRLRSRKGLAQMPIDGNFISSLATLPDCAGVALGVDRLVMLACEKKHIDDVIS
FTIKGA
>Mature_325_residues
SWKPSASIKELKQRAKILQAIRHFFITRGLLEVETPILSQAGVTDIHLFCFQTHFMGPQISSSKPEAQGTPLYLQTSPEF
HMKRLLSAGSGCIFQISKVFRNEESGRFHNPEFTLLEWYRVGFDHFRLMDEMDQLLQFILKSETARRCTYQNAFLEVLAV
DPLTASLEQLKTSGEKLNLGEVLSEEPDRDTVLQLLFCIAIEPVIAQHKPCFIYNFPASQAALARISPTDKRVAERFEVY
FQGIELANGFHELSDGQEQLRRFQADNRLRSRKGLAQMPIDGNFISSLATLPDCAGVALGVDRLVMLACEKKHIDDVISF
TIKGA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87082379, Length=326, Percent_Identity=57.6687116564417, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1790571, Length=340, Percent_Identity=28.2352941176471, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1789256, Length=332, Percent_Identity=28.0120481927711, Blast_Score=131, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6324256, Length=350, Percent_Identity=28, Blast_Score=108, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6320242, Length=337, Percent_Identity=26.1127596439169, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24640851, Length=348, Percent_Identity=27.2988505747126, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24640849, Length=348, Percent_Identity=27.2988505747126, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 36885; Mature: 36754

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWKPSASIKELKQRAKILQAIRHFFITRGLLEVETPILSQAGVTDIHLFCFQTHFMGPQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHCCCCCHHHHHHHHHHHCCCC
ISSSKPEAQGTPLYLQTSPEFHMKRLLSAGSGCIFQISKVFRNEESGRFHNPEFTLLEWY
CCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHH
RVGFDHFRLMDEMDQLLQFILKSETARRCTYQNAFLEVLAVDPLTASLEQLKTSGEKLNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCH
GEVLSEEPDRDTVLQLLFCIAIEPVIAQHKPCFIYNFPASQAALARISPTDKRVAERFEV
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHH
YFQGIELANGFHELSDGQEQLRRFQADNRLRSRKGLAQMPIDGNFISSLATLPDCAGVAL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHH
GVDRLVMLACEKKHIDDVISFTIKGA
HHHHHHHHHHHHHHHHHHHHEEECCC
>Mature Secondary Structure 
SWKPSASIKELKQRAKILQAIRHFFITRGLLEVETPILSQAGVTDIHLFCFQTHFMGPQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHCCCCCHHHHHHHHHHHCCCC
ISSSKPEAQGTPLYLQTSPEFHMKRLLSAGSGCIFQISKVFRNEESGRFHNPEFTLLEWY
CCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHH
RVGFDHFRLMDEMDQLLQFILKSETARRCTYQNAFLEVLAVDPLTASLEQLKTSGEKLNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCH
GEVLSEEPDRDTVLQLLFCIAIEPVIAQHKPCFIYNFPASQAALARISPTDKRVAERFEV
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHH
YFQGIELANGFHELSDGQEQLRRFQADNRLRSRKGLAQMPIDGNFISSLATLPDCAGVAL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHH
GVDRLVMLACEKKHIDDVISFTIKGA
HHHHHHHHHHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA