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The map label for this gene is miaA

Identifier: 119946845

GI number: 119946845

Start: 3995986

End: 3996903

Strand: Reverse

Name: miaA

Synonym: Ping_3239

Alternate gene names: 119946845

Gene position: 3996903-3995986 (Counterclockwise)

Preceding gene: 119946846

Following gene: 119946844

Centisome position: 87.66

GC content: 44.12

Gene sequence:

>918_bases
ATGAATAACTATCCGACCGCCCTGTTTTTAATGGGGCCTACGGCTTCTGGAAAAACCGATTTAGCCATAAAATTAGCCTT
GCAATGTGACTGTGAAATTATCAGTGTTGATTCCGCGTTAATTTATAAAGGAATGGATATTGGTACGGCCAAACCAAGCA
ATCACGAGCTGCAACAAGTGCCGCATGCTTTGGTTGATATTATTGACCCGCTTGAGTCCTACTCTGCCGGTGATTTTCGT
GAAGATGCATTAAGTTTAATGCAGGAGATCACCGATCGTGGTCATACTCCTTTATTAGTGGGGGGGACTATGCTGTATTA
CAAAGCCTTAGTCGACGGATTATCTCCGCTGCCCAGTGCCAATCCAAAAGTACGTGCGCAAATAGAGAAAGAAGCGCTGG
AAAATGGCTGGCAGGCTTTACATGATAAGCTTGAGCAGATAGACCCCGTTTCAGCTGCACGTATCCATGTGAATGATCCT
CAGCGTTTAGCGCGTGCCCTTGAGGTTTTCCGCATATCAGGCAAAAGCTTAACTGAACTGACAAAAGTGAAAAGTGATCC
TATTCCCTATAATATTAAACAATTTGCCATCGCACCCCTTGAAAAAAGCGTCTTACATGCGCGTATCGAGCAACGTTTTG
AACTAATGCTTGAATCCGGGTTTGAGCAGGAAGTACGCAAACTTTATCAACGTGGTGATTTGCATTTAGATTTACCCTCA
ATGCGCTGTGTTGGTTACCGGCAAATGTGGGAATATTTACAAGGCACAATGGATTATGAGGAAATGCGCTTTCGTGGCAT
TGTAGCAACGAGACAACTCGCTAAACGGCAAATGACCTGGTTACGCGGCTGGCAAAATGTAACTTGGCTTGAAACAGGTC
ATGCTGATAATTTTGAACGCATCAAGGCGATGTTATAA

Upstream 100 bases:

>100_bases
TTTGACTTATTACTTTCGTTGTTCTCGGAAAAGCTTAGTGACTGGCAGAAACAATTATTTAGAGTGCCCGATTTATCATT
ATTAGTACAAGGTTTTTCAC

Downstream 100 bases:

>100_bases
GCCGTAATTAAGCCATTTTCCCTACGGCAAAACTTCTTATTAAAAAGACTGGGGTTACGCGGCTGGCAGGATGTGATCTG
GCTTGAAACTAACGATGCGA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNNYPTALFLMGPTASGKTDLAIKLALQCDCEIISVDSALIYKGMDIGTAKPSNHELQQVPHALVDIIDPLESYSAGDFR
EDALSLMQEITDRGHTPLLVGGTMLYYKALVDGLSPLPSANPKVRAQIEKEALENGWQALHDKLEQIDPVSAARIHVNDP
QRLARALEVFRISGKSLTELTKVKSDPIPYNIKQFAIAPLEKSVLHARIEQRFELMLESGFEQEVRKLYQRGDLHLDLPS
MRCVGYRQMWEYLQGTMDYEEMRFRGIVATRQLAKRQMTWLRGWQNVTWLETGHADNFERIKAML

Sequences:

>Translated_305_residues
MNNYPTALFLMGPTASGKTDLAIKLALQCDCEIISVDSALIYKGMDIGTAKPSNHELQQVPHALVDIIDPLESYSAGDFR
EDALSLMQEITDRGHTPLLVGGTMLYYKALVDGLSPLPSANPKVRAQIEKEALENGWQALHDKLEQIDPVSAARIHVNDP
QRLARALEVFRISGKSLTELTKVKSDPIPYNIKQFAIAPLEKSVLHARIEQRFELMLESGFEQEVRKLYQRGDLHLDLPS
MRCVGYRQMWEYLQGTMDYEEMRFRGIVATRQLAKRQMTWLRGWQNVTWLETGHADNFERIKAML
>Mature_305_residues
MNNYPTALFLMGPTASGKTDLAIKLALQCDCEIISVDSALIYKGMDIGTAKPSNHELQQVPHALVDIIDPLESYSAGDFR
EDALSLMQEITDRGHTPLLVGGTMLYYKALVDGLSPLPSANPKVRAQIEKEALENGWQALHDKLEQIDPVSAARIHVNDP
QRLARALEVFRISGKSLTELTKVKSDPIPYNIKQFAIAPLEKSVLHARIEQRFELMLESGFEQEVRKLYQRGDLHLDLPS
MRCVGYRQMWEYLQGTMDYEEMRFRGIVATRQLAKRQMTWLRGWQNVTWLETGHADNFERIKAML

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=29.2604501607717, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI1790613, Length=290, Percent_Identity=66.551724137931, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25144712, Length=271, Percent_Identity=28.7822878228782, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324848, Length=295, Percent_Identity=27.1186440677966, Blast_Score=115, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24649830, Length=236, Percent_Identity=29.6610169491525, Blast_Score=108, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_PSYIN (A1SZL1)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944525.1
- ProteinModelPortal:   A1SZL1
- SMR:   A1SZL1
- STRING:   A1SZL1
- GeneID:   4626337
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3239
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   A1SZL1
- BioCyc:   PING357804:PING_3239-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34655; Mature: 34655

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNYPTALFLMGPTASGKTDLAIKLALQCDCEIISVDSALIYKGMDIGTAKPSNHELQQV
CCCCCCEEEEECCCCCCCCCEEEEEEEECCCEEEECCHHHHHCCCCCCCCCCCCCHHHHH
PHALVDIIDPLESYSAGDFREDALSLMQEITDRGHTPLLVGGTMLYYKALVDGLSPLPSA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCCC
NPKVRAQIEKEALENGWQALHDKLEQIDPVSAARIHVNDPQRLARALEVFRISGKSLTEL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCHHHHH
TKVKSDPIPYNIKQFAIAPLEKSVLHARIEQRFELMLESGFEQEVRKLYQRGDLHLDLPS
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEECCC
MRCVGYRQMWEYLQGTMDYEEMRFRGIVATRQLAKRQMTWLRGWQNVTWLETGHADNFER
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHH
IKAML
HHHCC
>Mature Secondary Structure
MNNYPTALFLMGPTASGKTDLAIKLALQCDCEIISVDSALIYKGMDIGTAKPSNHELQQV
CCCCCCEEEEECCCCCCCCCEEEEEEEECCCEEEECCHHHHHCCCCCCCCCCCCCHHHHH
PHALVDIIDPLESYSAGDFREDALSLMQEITDRGHTPLLVGGTMLYYKALVDGLSPLPSA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCCC
NPKVRAQIEKEALENGWQALHDKLEQIDPVSAARIHVNDPQRLARALEVFRISGKSLTEL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCHHHHH
TKVKSDPIPYNIKQFAIAPLEKSVLHARIEQRFELMLESGFEQEVRKLYQRGDLHLDLPS
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEECCC
MRCVGYRQMWEYLQGTMDYEEMRFRGIVATRQLAKRQMTWLRGWQNVTWLETGHADNFER
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHH
IKAML
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA