Click here to switch to the map view.

The map label for this gene is purH

Identifier: 119946826

GI number: 119946826

Start: 3975058

End: 3976650

Strand: Reverse

Name: purH

Synonym: Ping_3220

Alternate gene names: 119946826

Gene position: 3976650-3975058 (Counterclockwise)

Preceding gene: 119946827

Following gene: 119946825

Centisome position: 87.21

GC content: 43.5

Gene sequence:

>1593_bases
ATGGAAAATAGCCGACCAATTAAACGTGCGCTGCTAAGCGTTTCAGATAAAGCTGGAATTATTGAATTCGCCAAAGAGTT
ATCTGCAAGAGGCGTTGAAATCCTTTCTACTGGTGGTACTTGTAAATTACTTGCTGAGAATGACATAAAAGTGACTGAAG
TATCTGACTATACGGGGTTTCCTGAAATGATGGATGGGCGTGTTAAAACCTTACACCCTAAAATTCATGGTGGAATTCTT
GCTCGTCGCGGTATTGATGAAGTCATTATGTCCGAAAATGATATTGCTCCTATTGATTTAGTAGTTGTCAATCTTTATCC
CTTTGCTGAAACCGTTGCCCGTCCGGATTGTTCTTTAGAAGACGCCATTGAAAACATCGATATCGGTGGTCCAACTATGG
TGCGTGCCGCTGCTAAGAATCATAAAGATGTGGGAATTGTTGTTAACGCAGGTGATTACCCCCGTGTGCTTAAAGAGATG
CAGGAAAATAACAATAGCTTGGCTTATAAAACTCGCTTTGATCTTGCTATCGCAGCTTATGAGCATACCGCGCAATATGA
CGGTATGATCGCGAATTATTTCGGTACTATGGTGCCAAGCTACGGTGAGAATAGCGAAGGTGATCTTGAGTCTAAATTCC
CTCGTACCATCAACATGCAGTTTAAGAAAAAGCAAGATATGCGTTACGGTGAAAATAGCCACCAAAGTGCCGCTTTTTAT
GTGGAAGATGATATTCAAGAAGCCTCTGTTTCTACCGCGACTCAGCTACAGGGTAAAGCCCTTTCTTACAATAATATTGC
AGATACTGATGCGGCTCTTGAGTGTGTTAAAGAGTTTTCGGAGCCGGCTTGTGTAATCGTTAAACATTCTAACCCTTGTG
GTGTTGCTGTTGCAGGCAATATTCTTGATGCCTATGAAGGTGCTTACAAAACAGACCCGACATCTGCTTTTGGTGGCATT
ATTGCCTTTAACCGTGAGCTTGATGCAAAGACCGCAGAAGCCATTGTTTCTCGCCAATTTGTTGAGGTGATTATTGCGCC
TAGCGTTAGCCCGGAAGCTGCAAAAATTGTAGCGACTAAGAAAAACTTACGTTTGTTAGCCTGTGGAGAATGGTCTGACA
AAACGACCCAGTTTGATATCAAGCGTGTTAATGGCGGCTTATTAGTACAGGACAGAGATCAAGGTATGGTCGGTTTAGAG
GACCTTAAAGTGGTGACTAAACGTCAACCGACGGAAGCTGAATTAAAGGATCTGTTATTCAGCTGGAAAGTGGCTAAATT
TGTGAAATCAAATGCGATTGTTTATGTTAAAAACAATGCGACAGTGGGTGTTGGCGCAGGGCAAATGAGCCGAGTTTACA
GTGCGAAAGTCGCAGGTATCAAAGCCGCCGATGAAAACCTCGTCGTTGCAGGTTCAGTCATGTCATCGGATGCCTTTTTT
CCTTTCCGTGATGGTATTGATGCCGCCGCTGAAGCCGGTATTAGTTGCGTGATTCAACCTGGTGGTTCAATGCGTGACAA
TGAAGTTATTGCAGCAGCCGACGAACATGGTATGGCAATGGTATTTACAGGAATGCGTCACTTCCGCCATTAA

Upstream 100 bases:

>100_bases
ATCGTTTTCGGGTAGAATGCTCATCTTAGTGCTTTTTAAGTTAAAAAAACCAATGACTGGTTTTGCTTATTGAACAATTA
ACCGAGGGATTTATCAGACG

Downstream 100 bases:

>100_bases
TCGGCTCATTTATTCGTCTGTAGATGCGATCTCTGCGTTAGAGGTGCTCAATAATTCTTCAGGAAAGAATTATAACATCC
GTAGGATGGCCCTTTAGGGC

Product: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MENSRPIKRALLSVSDKAGIIEFAKELSARGVEILSTGGTCKLLAENDIKVTEVSDYTGFPEMMDGRVKTLHPKIHGGIL
ARRGIDEVIMSENDIAPIDLVVVNLYPFAETVARPDCSLEDAIENIDIGGPTMVRAAAKNHKDVGIVVNAGDYPRVLKEM
QENNNSLAYKTRFDLAIAAYEHTAQYDGMIANYFGTMVPSYGENSEGDLESKFPRTINMQFKKKQDMRYGENSHQSAAFY
VEDDIQEASVSTATQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHSNPCGVAVAGNILDAYEGAYKTDPTSAFGGI
IAFNRELDAKTAEAIVSRQFVEVIIAPSVSPEAAKIVATKKNLRLLACGEWSDKTTQFDIKRVNGGLLVQDRDQGMVGLE
DLKVVTKRQPTEAELKDLLFSWKVAKFVKSNAIVYVKNNATVGVGAGQMSRVYSAKVAGIKAADENLVVAGSVMSSDAFF
PFRDGIDAAAEAGISCVIQPGGSMRDNEVIAAADEHGMAMVFTGMRHFRH

Sequences:

>Translated_530_residues
MENSRPIKRALLSVSDKAGIIEFAKELSARGVEILSTGGTCKLLAENDIKVTEVSDYTGFPEMMDGRVKTLHPKIHGGIL
ARRGIDEVIMSENDIAPIDLVVVNLYPFAETVARPDCSLEDAIENIDIGGPTMVRAAAKNHKDVGIVVNAGDYPRVLKEM
QENNNSLAYKTRFDLAIAAYEHTAQYDGMIANYFGTMVPSYGENSEGDLESKFPRTINMQFKKKQDMRYGENSHQSAAFY
VEDDIQEASVSTATQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHSNPCGVAVAGNILDAYEGAYKTDPTSAFGGI
IAFNRELDAKTAEAIVSRQFVEVIIAPSVSPEAAKIVATKKNLRLLACGEWSDKTTQFDIKRVNGGLLVQDRDQGMVGLE
DLKVVTKRQPTEAELKDLLFSWKVAKFVKSNAIVYVKNNATVGVGAGQMSRVYSAKVAGIKAADENLVVAGSVMSSDAFF
PFRDGIDAAAEAGISCVIQPGGSMRDNEVIAAADEHGMAMVFTGMRHFRH
>Mature_530_residues
MENSRPIKRALLSVSDKAGIIEFAKELSARGVEILSTGGTCKLLAENDIKVTEVSDYTGFPEMMDGRVKTLHPKIHGGIL
ARRGIDEVIMSENDIAPIDLVVVNLYPFAETVARPDCSLEDAIENIDIGGPTMVRAAAKNHKDVGIVVNAGDYPRVLKEM
QENNNSLAYKTRFDLAIAAYEHTAQYDGMIANYFGTMVPSYGENSEGDLESKFPRTINMQFKKKQDMRYGENSHQSAAFY
VEDDIQEASVSTATQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHSNPCGVAVAGNILDAYEGAYKTDPTSAFGGI
IAFNRELDAKTAEAIVSRQFVEVIIAPSVSPEAAKIVATKKNLRLLACGEWSDKTTQFDIKRVNGGLLVQDRDQGMVGLE
DLKVVTKRQPTEAELKDLLFSWKVAKFVKSNAIVYVKNNATVGVGAGQMSRVYSAKVAGIKAADENLVVAGSVMSSDAFF
PFRDGIDAAAEAGISCVIQPGGSMRDNEVIAAADEHGMAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=488, Percent_Identity=37.5, Blast_Score=264, Evalue=2e-70,
Organism=Escherichia coli, GI1790439, Length=531, Percent_Identity=75.7062146892655, Blast_Score=848, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=510, Percent_Identity=32.7450980392157, Blast_Score=234, Evalue=8e-62,
Organism=Caenorhabditis elegans, GI71985574, Length=340, Percent_Identity=27.0588235294118, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71985556, Length=119, Percent_Identity=37.8151260504202, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323056, Length=618, Percent_Identity=32.8478964401294, Blast_Score=266, Evalue=7e-72,
Organism=Saccharomyces cerevisiae, GI6323768, Length=486, Percent_Identity=35.1851851851852, Blast_Score=245, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24649832, Length=618, Percent_Identity=33.9805825242718, Blast_Score=294, Evalue=1e-79,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_PSYIN (A1SZJ2)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944506.1
- ProteinModelPortal:   A1SZJ2
- SMR:   A1SZJ2
- STRING:   A1SZJ2
- GeneID:   4624653
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3220
- eggNOG:   COG0138
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- PhylomeDB:   A1SZJ2
- BioCyc:   PING357804:PING_3220-MONOMER
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 57478; Mature: 57478

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENSRPIKRALLSVSDKAGIIEFAKELSARGVEILSTGGTCKLLAENDIKVTEVSDYTGF
CCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCEEEEEECCCEEEEEECCCCCC
PEMMDGRVKTLHPKIHGGILARRGIDEVIMSENDIAPIDLVVVNLYPFAETVARPDCSLE
HHHHCCCEEEECCHHHCCCHHHCCCHHHHHCCCCCCCEEEEEEEEEHHHHHHCCCCCCHH
DAIENIDIGGPTMVRAAAKNHKDVGIVVNAGDYPRVLKEMQENNNSLAYKTRFDLAIAAY
HHHHHCCCCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEEEEEEEEEE
EHTAQYDGMIANYFGTMVPSYGENSEGDLESKFPRTINMQFKKKQDMRYGENSHQSAAFY
HHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHCCCCEEEEEEHHHHHCCCCCCCCCCEEEE
VEDDIQEASVSTATQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHSNPCGVAVAGN
EECCCHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEECC
ILDAYEGAYKTDPTSAFGGIIAFNRELDAKTAEAIVSRQFVEVIIAPSVSPEAAKIVATK
HHHHHCCCCCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEC
KNLRLLACGEWSDKTTQFDIKRVNGGLLVQDRDQGMVGLEDLKVVTKRQPTEAELKDLLF
CCEEEEEECCCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHH
SWKVAKFVKSNAIVYVKNNATVGVGAGQMSRVYSAKVAGIKAADENLVVAGSVMSSDAFF
HHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHCCEEECCCCEEEEEEEECCCCCC
PFRDGIDAAAEAGISCVIQPGGSMRDNEVIAAADEHGMAMVFTGMRHFRH
CCCCCCCHHHHCCCEEEECCCCCCCCCCEEEEECCCCEEEEEECHHHHCC
>Mature Secondary Structure
MENSRPIKRALLSVSDKAGIIEFAKELSARGVEILSTGGTCKLLAENDIKVTEVSDYTGF
CCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCEEEEEECCCEEEEEECCCCCC
PEMMDGRVKTLHPKIHGGILARRGIDEVIMSENDIAPIDLVVVNLYPFAETVARPDCSLE
HHHHCCCEEEECCHHHCCCHHHCCCHHHHHCCCCCCCEEEEEEEEEHHHHHHCCCCCCHH
DAIENIDIGGPTMVRAAAKNHKDVGIVVNAGDYPRVLKEMQENNNSLAYKTRFDLAIAAY
HHHHHCCCCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEEEEEEEEEE
EHTAQYDGMIANYFGTMVPSYGENSEGDLESKFPRTINMQFKKKQDMRYGENSHQSAAFY
HHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHCCCCEEEEEEHHHHHCCCCCCCCCCEEEE
VEDDIQEASVSTATQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHSNPCGVAVAGN
EECCCHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEECC
ILDAYEGAYKTDPTSAFGGIIAFNRELDAKTAEAIVSRQFVEVIIAPSVSPEAAKIVATK
HHHHHCCCCCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEC
KNLRLLACGEWSDKTTQFDIKRVNGGLLVQDRDQGMVGLEDLKVVTKRQPTEAELKDLLF
CCEEEEEECCCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHH
SWKVAKFVKSNAIVYVKNNATVGVGAGQMSRVYSAKVAGIKAADENLVVAGSVMSSDAFF
HHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHCCEEECCCCEEEEEEEECCCCCC
PFRDGIDAAAEAGISCVIQPGGSMRDNEVIAAADEHGMAMVFTGMRHFRH
CCCCCCCHHHHCCCEEEECCCCCCCCCCEEEEECCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA