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The map label for this gene is purD [H]

Identifier: 119946825

GI number: 119946825

Start: 3973550

End: 3974839

Strand: Reverse

Name: purD [H]

Synonym: Ping_3219

Alternate gene names: 119946825

Gene position: 3974839-3973550 (Counterclockwise)

Preceding gene: 119946826

Following gene: 119946816

Centisome position: 87.18

GC content: 46.51

Gene sequence:

>1290_bases
ATGAATGTTTTAATTATTGGCAGCGGCGGCCGTGAGCATGCGCTTGCGTGGAAAGTCGCTCAAAATAAACAAGTTAAAAA
TATCTTTGTTGCCCCGGGCAATGCAGGAACGGCATTAGAAGCAAAAGTAACTAATGTTGCCATTGATGTTGAAGATATTG
ACGGATTAGTCAAATTTGCTAAAGAAAATCGTGTTGAATTAACGATTGTCGGCCCCGAAGCACCCTTAGTTATCGGTGTT
GTAGATGCATTTACTGATGCCGGCCTTGCCTGTTTCGGTCCAACTAAGGGCGCGGCCCAGCTAGAAGGCTCAAAAGCGTT
CACTAAGGACTTTTTAGCACGTCATAATATCCCAACGGCCTGGTACCAAAACTTTACTGAAATTGAGCCTGCGATCGCTT
ATGTTCGTGAAAAGGGTGCACCGATCGTCATTAAAGCTGACGGTCTTGCGGCGGGTAAAGGCGTTATTGTAGCGATGACA
CTTGCCGATGCGGAAGAAGCTATTCATGACATGCTAGCGGGCAATGCATTTGGCTCTGCCGGGCACCGTGTTGTTATTGA
AGAGTTCTTAGACGGTGAAGAAGCTTCATTTATTGTGATGGTTGATGGCGAAAATATTGTTCCTATGGCCACCAGTCAAG
ATCATAAACGTGTCGGTGATGGTGATACCGGGCCAAATACGGGCGGTATGGGTGCATACTCTCCCGCGCCTGTAGTGACG
CCTGAAATGCATCAGCGCATAATGGACTTAGTCATTAACCCAACTGTTAAAGGCATGGCGGCTGAAGGTAATAGTTACAG
CGGTTTTCTTTATGCGGGTCTGATGATCATGGCCGATGGAACACCTAAAGTTATCGAATACAACTGTCGTTTTGGTGATC
CGGAAACACAACCGCTGTTGTTGCGCATGAAGTCTGATCTGGTCACCTTATGCCTGGCGGGTGTAAATGGCGAATTGGCT
GATATGACTGCTGAATTTGACCAACGTGCAGCGATCGGTATTGTCTTGGCGGCTGGTGGTTATCCTAATGAGTATAAAAA
AGGCTTAGTGATCGAAGGTTTGCCTGACCAGGAAATGGAAGGCCTCAAAGTTTTCCATGGTGGTACCAAAGAAGTTGATG
GCCAAATTGTTACCAATGGCGGACGTGTTTTGTGTGCGACAGCATTAGGCAATACAGTGACCGAGGCACAGCAAAAAGCT
TACGCTCTGGCTAAGACAATTAGCTGGGATGGTATGTTCTATCGTCATGATATTGCTCATCGTGCGATTGCTCGTGAGCA
GAAAAAATAA

Upstream 100 bases:

>100_bases
AGTACTTATCGTTATAAGCTGCGCACTTTTTCTTTGAGTTGAATCACCGGACATTGAGATAAACCCATTAAATCTATGGT
TGAATTAAGAAGGTTAAGTT

Downstream 100 bases:

>100_bases
GCTGAAAAGTTGAAAGGCTAGAAACCCCGAAGGCGGGAAGCTCGAAACTCGTAGTTCGAAGCTTGAATAAAAAGCCGATG
TTTTATCACATCGGCTTTTT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNVLIIGSGGREHALAWKVAQNKQVKNIFVAPGNAGTALEAKVTNVAIDVEDIDGLVKFAKENRVELTIVGPEAPLVIGV
VDAFTDAGLACFGPTKGAAQLEGSKAFTKDFLARHNIPTAWYQNFTEIEPAIAYVREKGAPIVIKADGLAAGKGVIVAMT
LADAEEAIHDMLAGNAFGSAGHRVVIEEFLDGEEASFIVMVDGENIVPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
PEMHQRIMDLVINPTVKGMAAEGNSYSGFLYAGLMIMADGTPKVIEYNCRFGDPETQPLLLRMKSDLVTLCLAGVNGELA
DMTAEFDQRAAIGIVLAAGGYPNEYKKGLVIEGLPDQEMEGLKVFHGGTKEVDGQIVTNGGRVLCATALGNTVTEAQQKA
YALAKTISWDGMFYRHDIAHRAIAREQKK

Sequences:

>Translated_429_residues
MNVLIIGSGGREHALAWKVAQNKQVKNIFVAPGNAGTALEAKVTNVAIDVEDIDGLVKFAKENRVELTIVGPEAPLVIGV
VDAFTDAGLACFGPTKGAAQLEGSKAFTKDFLARHNIPTAWYQNFTEIEPAIAYVREKGAPIVIKADGLAAGKGVIVAMT
LADAEEAIHDMLAGNAFGSAGHRVVIEEFLDGEEASFIVMVDGENIVPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
PEMHQRIMDLVINPTVKGMAAEGNSYSGFLYAGLMIMADGTPKVIEYNCRFGDPETQPLLLRMKSDLVTLCLAGVNGELA
DMTAEFDQRAAIGIVLAAGGYPNEYKKGLVIEGLPDQEMEGLKVFHGGTKEVDGQIVTNGGRVLCATALGNTVTEAQQKA
YALAKTISWDGMFYRHDIAHRAIAREQKK
>Mature_429_residues
MNVLIIGSGGREHALAWKVAQNKQVKNIFVAPGNAGTALEAKVTNVAIDVEDIDGLVKFAKENRVELTIVGPEAPLVIGV
VDAFTDAGLACFGPTKGAAQLEGSKAFTKDFLARHNIPTAWYQNFTEIEPAIAYVREKGAPIVIKADGLAAGKGVIVAMT
LADAEEAIHDMLAGNAFGSAGHRVVIEEFLDGEEASFIVMVDGENIVPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
PEMHQRIMDLVINPTVKGMAAEGNSYSGFLYAGLMIMADGTPKVIEYNCRFGDPETQPLLLRMKSDLVTLCLAGVNGELA
DMTAEFDQRAAIGIVLAAGGYPNEYKKGLVIEGLPDQEMEGLKVFHGGTKEVDGQIVTNGGRVLCATALGNTVTEAQQKA
YALAKTISWDGMFYRHDIAHRAIAREQKK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=429, Percent_Identity=48.7179487179487, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI4503915, Length=429, Percent_Identity=48.7179487179487, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI209869995, Length=429, Percent_Identity=48.7179487179487, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI209869993, Length=429, Percent_Identity=48.7179487179487, Blast_Score=420, Evalue=1e-117,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=73.3644859813084, Blast_Score=644, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=41.8823529411765, Blast_Score=316, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=42.0224719101124, Blast_Score=339, Evalue=5e-94,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=44.6511627906977, Blast_Score=351, Evalue=6e-97,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=44.6511627906977, Blast_Score=350, Evalue=1e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45574; Mature: 45574

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLIIGSGGREHALAWKVAQNKQVKNIFVAPGNAGTALEAKVTNVAIDVEDIDGLVKFA
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCEEEEEEEEEEEEHHHHHHHHHHH
KENRVELTIVGPEAPLVIGVVDAFTDAGLACFGPTKGAAQLEGSKAFTKDFLARHNIPTA
HCCCEEEEEECCCCCEEEEEEHHHCCCCEEEECCCCCCCEECCCHHHHHHHHHHCCCCHH
WYQNFTEIEPAIAYVREKGAPIVIKADGLAAGKGVIVAMTLADAEEAIHDMLAGNAFGSA
HHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCC
GHRVVIEEFLDGEEASFIVMVDGENIVPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
CCEEEEHHHCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PEMHQRIMDLVINPTVKGMAAEGNSYSGFLYAGLMIMADGTPKVIEYNCRFGDPETQPLL
HHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEEEEEECCCCCEEEEEECCCCCCCCEEE
LRMKSDLVTLCLAGVNGELADMTAEFDQRAAIGIVLAAGGYPNEYKKGLVIEGLPDQEME
EEEHHCEEEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCCHHHHCCEEEECCCCCCCC
GLKVFHGGTKEVDGQIVTNGGRVLCATALGNTVTEAQQKAYALAKTISWDGMFYRHDIAH
CEEEEECCCCCCCCEEEECCCEEEEEEHHCCHHHHHHHHHHHHHHEECCCCEEEHHHHHH
RAIAREQKK
HHHHHHCCC
>Mature Secondary Structure
MNVLIIGSGGREHALAWKVAQNKQVKNIFVAPGNAGTALEAKVTNVAIDVEDIDGLVKFA
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCEEEEEEEEEEEEHHHHHHHHHHH
KENRVELTIVGPEAPLVIGVVDAFTDAGLACFGPTKGAAQLEGSKAFTKDFLARHNIPTA
HCCCEEEEEECCCCCEEEEEEHHHCCCCEEEECCCCCCCEECCCHHHHHHHHHHCCCCHH
WYQNFTEIEPAIAYVREKGAPIVIKADGLAAGKGVIVAMTLADAEEAIHDMLAGNAFGSA
HHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCC
GHRVVIEEFLDGEEASFIVMVDGENIVPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
CCEEEEHHHCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PEMHQRIMDLVINPTVKGMAAEGNSYSGFLYAGLMIMADGTPKVIEYNCRFGDPETQPLL
HHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEEEEEECCCCCEEEEEECCCCCCCCEEE
LRMKSDLVTLCLAGVNGELADMTAEFDQRAAIGIVLAAGGYPNEYKKGLVIEGLPDQEME
EEEHHCEEEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCCHHHHCCEEEECCCCCCCC
GLKVFHGGTKEVDGQIVTNGGRVLCATALGNTVTEAQQKAYALAKTISWDGMFYRHDIAH
CEEEEECCCCCCCCEEEECCCEEEEEEHHCCHHHHHHHHHHHHHHEECCCCEEEHHHHHH
RAIAREQKK
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]