Click here to switch to the map view.

The map label for this gene is glgX [H]

Identifier: 119946688

GI number: 119946688

Start: 3768928

End: 3770988

Strand: Reverse

Name: glgX [H]

Synonym: Ping_3070

Alternate gene names: 119946688

Gene position: 3770988-3768928 (Counterclockwise)

Preceding gene: 119946689

Following gene: 119946686

Centisome position: 82.7

GC content: 41.29

Gene sequence:

>2061_bases
ATGTTTTCAGCTTCGAATGGTAAACCAGATAAGTTAGGCGTGACCTATAGCAGCAAAGGTGCCAATTTTTGTATTTATGC
GCGGCTGGCAGAAAGTGTCGAACTGCTTTTTTTTGAAGATAAAGATGCCGATGCAGCGAGCAATATATTTAAATTTTCAA
CTAATGATAATCGTACCGCTTATTATTGGCATATTTTTATTGAAGATGTAAAACCGGGACAACTCTATGGCTTTCGTGTC
AATGGACCTTATCGCCCCTATCAAGGAACGGCTTTTTCCCCAAAGAAAGTGTTATTAGACCCCTATGGATTATTGGTTGA
ACGGGGTGATCACTTTTCCCGTGAGGCAGCACTTGGCAGCGGCTCCAATATTGATACCTGTTTAAAAAGTGTGGTGGTTG
ATATAGATGATTATGATTGGCAGGAAGATCAACATCCTCGGCACTCTTTTTATAAAACAGTTATTTATGAAATGCATGTG
GGCGGTTTTACTCGCCATCAAAGTGCTAATATTGCAAAAAAAAAACGCGGTACTTATGCGGGCTTAATTGAAAAGATCCC
CTATTTAAAAGAACTGGGGGTAACCGCCGTTGAACTAATGCCGATTCATCAGTTCGATCCGGGTGAACATCACGCCGGAT
TGGCTAACTATTGGGGATATAGCCCGTTATCCTTTTTCGCAGTGCATCGCGAATACAGCTCAGACAGATCCAGGCTGGGC
CCCGTTAATGAATTTAGAGATATGGTAAAAGCCCTGCATGCGGCTGATATTGAAGTTATTTTGGATGTGGTTTATAACCA
TAGCGCAGAGGGTGGGGAAGGTGGCCCGTCTTTTTCGTTGCGTGGTTTTGATAACAATGATTATTACATCTTATCCAAAG
ATAGACAGCATTATATGAATTTTAGTGGTTGTGGTAATACCCTTAACGGTACACATTCCGTGGTTAGACGCATGATTATT
GACAGTTTGCACTTTTGGGTAGACAAAATGCATATAGATGGATTTCGTTTTGATTTGGCCTCTATTCTATCCCGTGATGA
GCTTGGTGCGCCTATGCTCAGCCCGCCGACGTTATTCAGTATTGATACTGATCCGATTTTGTCCTCAATTAAGCTGATAG
CAGAAGCATGGGATGCAGGGGGATTATATCAAGTGGGTAGTCTGGCAGGGCAGCGTTGGCGAGAATGGAACGGTCATTTT
AGAGATGATGTACGGCGTTTTGTGCGTGGCGATCAGGGTATGGTAAGTCAGTTTGCCAACCGTTTGATTGGCAGTCCGGA
TATTTACGGTGAACATCACTTTGAACCGGAAAAATCGGTAAACTTTATTACCTGCCATGACGGCTTTACGCTTAATGATC
TGGTCAGTTATAATAACAAACATAATTTGCCCAATGGTGAAAATAATCTCGATGGATGTGATTATAATTTCAGCTGGAAT
CATGGGGTTGAAGGTGAAACGAATGATCCTAAAATCCTTCAGCTGCGCAATAAGCAAATCAAAAACATGCTGACGATAAC
TTTGCTCTCTTTAGGTACGCCGATGATTTTAATGGGTGATGAAATAGGGCATTCGCAAAAAGGCAATAATAATGGCTATT
GTCAGGATAATCCGGCATTTTGGTTTGATTGGAGTCAGATTAAAAAGAACAGGGCGCTTTTTGACTTTACTAAGGGTTTA
ATTAAACAGCGTACCGCTATCTATCTCAATCCCAGTTTCCGCTATTCTTTGCATGATATTATTCTACGGTCAGACATTCA
TTGGCATGGTGTGAAACTCAATCAACCTGATTGGAGTGAGTGTTCTCATAGCATTGCCATGGAAAGTACACACCCTCACT
CAGGGGTTGTCTCTTATGTTATTTTTAATGCTTTTTGGGAAGTACTTAACTTTGAATTACCAATATCTGCATGCGGGAAA
TGGTTGCGAATCGTTGATACTTCCTTAGTGGATGGTAACGATATTTATAAGAGTGGCGATAAAAAATACTGGTTTAACGA
TCAATACCTTGTTGCACCACGATCCGTGGTGATTTTGATTGGAGAAACACCCGATGATTAA

Upstream 100 bases:

>100_bases
ACGGCTATTATTATCAGATGGCCTAATTCTATGTAACTCCCTCTAAAAATCATTTTCTTAGATGTGCCTGTTTTATTTTT
CACTGTGATCTGGAGTAAGT

Downstream 100 bases:

>100_bases
TATAAATCATCATACTACTGTCTTCAGACTTAAGTTTGTTACAAAAAAATGCAGCACCACAGAGCCCACGCACACTTCAT
CAGACTGAGAAACGTTGTTT

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MFSASNGKPDKLGVTYSSKGANFCIYARLAESVELLFFEDKDADAASNIFKFSTNDNRTAYYWHIFIEDVKPGQLYGFRV
NGPYRPYQGTAFSPKKVLLDPYGLLVERGDHFSREAALGSGSNIDTCLKSVVVDIDDYDWQEDQHPRHSFYKTVIYEMHV
GGFTRHQSANIAKKKRGTYAGLIEKIPYLKELGVTAVELMPIHQFDPGEHHAGLANYWGYSPLSFFAVHREYSSDRSRLG
PVNEFRDMVKALHAADIEVILDVVYNHSAEGGEGGPSFSLRGFDNNDYYILSKDRQHYMNFSGCGNTLNGTHSVVRRMII
DSLHFWVDKMHIDGFRFDLASILSRDELGAPMLSPPTLFSIDTDPILSSIKLIAEAWDAGGLYQVGSLAGQRWREWNGHF
RDDVRRFVRGDQGMVSQFANRLIGSPDIYGEHHFEPEKSVNFITCHDGFTLNDLVSYNNKHNLPNGENNLDGCDYNFSWN
HGVEGETNDPKILQLRNKQIKNMLTITLLSLGTPMILMGDEIGHSQKGNNNGYCQDNPAFWFDWSQIKKNRALFDFTKGL
IKQRTAIYLNPSFRYSLHDIILRSDIHWHGVKLNQPDWSECSHSIAMESTHPHSGVVSYVIFNAFWEVLNFELPISACGK
WLRIVDTSLVDGNDIYKSGDKKYWFNDQYLVAPRSVVILIGETPDD

Sequences:

>Translated_686_residues
MFSASNGKPDKLGVTYSSKGANFCIYARLAESVELLFFEDKDADAASNIFKFSTNDNRTAYYWHIFIEDVKPGQLYGFRV
NGPYRPYQGTAFSPKKVLLDPYGLLVERGDHFSREAALGSGSNIDTCLKSVVVDIDDYDWQEDQHPRHSFYKTVIYEMHV
GGFTRHQSANIAKKKRGTYAGLIEKIPYLKELGVTAVELMPIHQFDPGEHHAGLANYWGYSPLSFFAVHREYSSDRSRLG
PVNEFRDMVKALHAADIEVILDVVYNHSAEGGEGGPSFSLRGFDNNDYYILSKDRQHYMNFSGCGNTLNGTHSVVRRMII
DSLHFWVDKMHIDGFRFDLASILSRDELGAPMLSPPTLFSIDTDPILSSIKLIAEAWDAGGLYQVGSLAGQRWREWNGHF
RDDVRRFVRGDQGMVSQFANRLIGSPDIYGEHHFEPEKSVNFITCHDGFTLNDLVSYNNKHNLPNGENNLDGCDYNFSWN
HGVEGETNDPKILQLRNKQIKNMLTITLLSLGTPMILMGDEIGHSQKGNNNGYCQDNPAFWFDWSQIKKNRALFDFTKGL
IKQRTAIYLNPSFRYSLHDIILRSDIHWHGVKLNQPDWSECSHSIAMESTHPHSGVVSYVIFNAFWEVLNFELPISACGK
WLRIVDTSLVDGNDIYKSGDKKYWFNDQYLVAPRSVVILIGETPDD
>Mature_686_residues
MFSASNGKPDKLGVTYSSKGANFCIYARLAESVELLFFEDKDADAASNIFKFSTNDNRTAYYWHIFIEDVKPGQLYGFRV
NGPYRPYQGTAFSPKKVLLDPYGLLVERGDHFSREAALGSGSNIDTCLKSVVVDIDDYDWQEDQHPRHSFYKTVIYEMHV
GGFTRHQSANIAKKKRGTYAGLIEKIPYLKELGVTAVELMPIHQFDPGEHHAGLANYWGYSPLSFFAVHREYSSDRSRLG
PVNEFRDMVKALHAADIEVILDVVYNHSAEGGEGGPSFSLRGFDNNDYYILSKDRQHYMNFSGCGNTLNGTHSVVRRMII
DSLHFWVDKMHIDGFRFDLASILSRDELGAPMLSPPTLFSIDTDPILSSIKLIAEAWDAGGLYQVGSLAGQRWREWNGHF
RDDVRRFVRGDQGMVSQFANRLIGSPDIYGEHHFEPEKSVNFITCHDGFTLNDLVSYNNKHNLPNGENNLDGCDYNFSWN
HGVEGETNDPKILQLRNKQIKNMLTITLLSLGTPMILMGDEIGHSQKGNNNGYCQDNPAFWFDWSQIKKNRALFDFTKGL
IKQRTAIYLNPSFRYSLHDIILRSDIHWHGVKLNQPDWSECSHSIAMESTHPHSGVVSYVIFNAFWEVLNFELPISACGK
WLRIVDTSLVDGNDIYKSGDKKYWFNDQYLVAPRSVVILIGETPDD

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=582, Percent_Identity=43.298969072165, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1789839, Length=373, Percent_Identity=26.2734584450402, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320826, Length=199, Percent_Identity=31.6582914572864, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 77969; Mature: 77969

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSASNGKPDKLGVTYSSKGANFCIYARLAESVELLFFEDKDADAASNIFKFSTNDNRTA
CCCCCCCCCCEEEEEECCCCCCEEEEEEHHCCEEEEEEECCCCCCCCCEEEEECCCCCEE
YYWHIFIEDVKPGQLYGFRVNGPYRPYQGTAFSPKKVLLDPYGLLVERGDHFSREAALGS
EEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCEEECCHHHEEECCCCCCHHHCCCC
GSNIDTCLKSVVVDIDDYDWQEDQHPRHSFYKTVIYEMHVGGFTRHQSANIAKKKRGTYA
CCCHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCCCHHHHHCCCHH
GLIEKIPYLKELGVTAVELMPIHQFDPGEHHAGLANYWGYSPLSFFAVHREYSSDRSRLG
HHHHHCCHHHHCCCEEEEEEEEECCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCHHHCC
PVNEFRDMVKALHAADIEVILDVVYNHSAEGGEGGPSFSLRGFDNNDYYILSKDRQHYMN
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEECCHHHHCC
FSGCGNTLNGTHSVVRRMIIDSLHFWVDKMHIDGFRFDLASILSRDELGAPMLSPPTLFS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHCCCCCCCCCEEEE
IDTDPILSSIKLIAEAWDAGGLYQVGSLAGQRWREWNGHFRDDVRRFVRGDQGMVSQFAN
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHH
RLIGSPDIYGEHHFEPEKSVNFITCHDGFTLNDLVSYNNKHNLPNGENNLDGCDYNFSWN
HHCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
HGVEGETNDPKILQLRNKQIKNMLTITLLSLGTPMILMGDEIGHSQKGNNNGYCQDNPAF
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCE
WFDWSQIKKNRALFDFTKGLIKQRTAIYLNPSFRYSLHDIILRSDIHWHGVKLNQPDWSE
EEEHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCHHH
CSHSIAMESTHPHSGVVSYVIFNAFWEVLNFELPISACGKWLRIVDTSLVDGNDIYKSGD
HHCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCC
KKYWFNDQYLVAPRSVVILIGETPDD
CEEEECCEEEECCCEEEEEEECCCCC
>Mature Secondary Structure
MFSASNGKPDKLGVTYSSKGANFCIYARLAESVELLFFEDKDADAASNIFKFSTNDNRTA
CCCCCCCCCCEEEEEECCCCCCEEEEEEHHCCEEEEEEECCCCCCCCCEEEEECCCCCEE
YYWHIFIEDVKPGQLYGFRVNGPYRPYQGTAFSPKKVLLDPYGLLVERGDHFSREAALGS
EEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCEEECCHHHEEECCCCCCHHHCCCC
GSNIDTCLKSVVVDIDDYDWQEDQHPRHSFYKTVIYEMHVGGFTRHQSANIAKKKRGTYA
CCCHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCCCHHHHHCCCHH
GLIEKIPYLKELGVTAVELMPIHQFDPGEHHAGLANYWGYSPLSFFAVHREYSSDRSRLG
HHHHHCCHHHHCCCEEEEEEEEECCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCHHHCC
PVNEFRDMVKALHAADIEVILDVVYNHSAEGGEGGPSFSLRGFDNNDYYILSKDRQHYMN
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEECCHHHHCC
FSGCGNTLNGTHSVVRRMIIDSLHFWVDKMHIDGFRFDLASILSRDELGAPMLSPPTLFS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHCCCCCCCCCEEEE
IDTDPILSSIKLIAEAWDAGGLYQVGSLAGQRWREWNGHFRDDVRRFVRGDQGMVSQFAN
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHH
RLIGSPDIYGEHHFEPEKSVNFITCHDGFTLNDLVSYNNKHNLPNGENNLDGCDYNFSWN
HHCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
HGVEGETNDPKILQLRNKQIKNMLTITLLSLGTPMILMGDEIGHSQKGNNNGYCQDNPAF
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCE
WFDWSQIKKNRALFDFTKGLIKQRTAIYLNPSFRYSLHDIILRSDIHWHGVKLNQPDWSE
EEEHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCHHH
CSHSIAMESTHPHSGVVSYVIFNAFWEVLNFELPISACGKWLRIVDTSLVDGNDIYKSGD
HHCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCC
KKYWFNDQYLVAPRSVVILIGETPDD
CEEEECCEEEECCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]