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The map label for this gene is queF

Identifier: 119946399

GI number: 119946399

Start: 3410614

End: 3411471

Strand: Direct

Name: queF

Synonym: Ping_2773

Alternate gene names: 119946399

Gene position: 3410614-3411471 (Clockwise)

Preceding gene: 119946397

Following gene: 119946400

Centisome position: 74.8

GC content: 38.69

Gene sequence:

>858_bases
ATGACTAATAAACCACTCTACGATCAAGATAGCACACTAAAAAATCTGTCTCTCGGAAAAATAACCGAATATAAAAGCAG
CTACGATCCAACATTATTACAAGCTGTGCCACGCAGTTTAAACCGTAATGAACTGCAACTTTCAGAACATAATCTGCCCT
TTTATGGTGTTGATTTGTGGAATATTTACGAGCTTTCCTGGTTAAACAGTAAAGGCAAACCTGTTGTTGCAACCGGGGTG
GTAAAAGTCCCCTTTGACAGTAAAAACTTAATTGAATCAAAATCCTTCAAGTTATATTTAAACAGTTTTAACCAAAGCAA
GTTTGTTTCTATCGAGGCGGTACAGAAAGTGTTAACGGCCGATCTGTCGCATTGTGCCGATAAAGCCGTTAGCGTTGAAC
TTCACACTGATTTAGATAACTTCAGCGATAAGCTGGGTACTTTTAGCGGACAGTGTTTAGATGTCTTAGACATTGAAATT
GATAACTACCAGCTGAACGCAGACTATCTTCAGGACCTGTCCTCACAGGAGCAGGTAACCGAAACGCTGTATTCTCATTT
ATTAAAGTCAAACTGTTTAATTACCAGTCAACCGGATTGGGCCAGTATCGAAATCAGTTATACCGGAAAAAAATTAGATC
GTGAGAAATTATTGCGCTATTTAATCTCTTTCCGTCAACATAACGAGTTTCATGAGCAGTGTGTTGAACGAATCTATTGT
GACATCATGAAATTTGGACAAATTGATTCGCTTTGTGTTTATGCGCGTTATACTCGCCGGGGCGGATTAGATATTAACCC
GCTGCGTACCACGGAACACATTAATGAGATTAATAATCTTCGTTTATTACGACAATAA

Upstream 100 bases:

>100_bases
GAATAGCTGTGTTTGCGTTGTATGATTCATAGCTCGAATAATTCTTTATTTTGTTTACAATACTGCCATTGTATTGTTTT
AAAAAATAAAAGCGAAAAAA

Downstream 100 bases:

>100_bases
AAATTAGCTGTTATGAGATATGTCAGTCAATACTTTCGCGCTGTTTGTGCGCTATTTTTAGTTCAGGTCAGTCAATCAAG
CGGGGCGTTGGAATTAACCG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTNKPLYDQDSTLKNLSLGKITEYKSSYDPTLLQAVPRSLNRNELQLSEHNLPFYGVDLWNIYELSWLNSKGKPVVATGV
VKVPFDSKNLIESKSFKLYLNSFNQSKFVSIEAVQKVLTADLSHCADKAVSVELHTDLDNFSDKLGTFSGQCLDVLDIEI
DNYQLNADYLQDLSSQEQVTETLYSHLLKSNCLITSQPDWASIEISYTGKKLDREKLLRYLISFRQHNEFHEQCVERIYC
DIMKFGQIDSLCVYARYTRRGGLDINPLRTTEHINEINNLRLLRQ

Sequences:

>Translated_285_residues
MTNKPLYDQDSTLKNLSLGKITEYKSSYDPTLLQAVPRSLNRNELQLSEHNLPFYGVDLWNIYELSWLNSKGKPVVATGV
VKVPFDSKNLIESKSFKLYLNSFNQSKFVSIEAVQKVLTADLSHCADKAVSVELHTDLDNFSDKLGTFSGQCLDVLDIEI
DNYQLNADYLQDLSSQEQVTETLYSHLLKSNCLITSQPDWASIEISYTGKKLDREKLLRYLISFRQHNEFHEQCVERIYC
DIMKFGQIDSLCVYARYTRRGGLDINPLRTTEHINEINNLRLLRQ
>Mature_284_residues
TNKPLYDQDSTLKNLSLGKITEYKSSYDPTLLQAVPRSLNRNELQLSEHNLPFYGVDLWNIYELSWLNSKGKPVVATGVV
KVPFDSKNLIESKSFKLYLNSFNQSKFVSIEAVQKVLTADLSHCADKAVSVELHTDLDNFSDKLGTFSGQCLDVLDIEID
NYQLNADYLQDLSSQEQVTETLYSHLLKSNCLITSQPDWASIEISYTGKKLDREKLLRYLISFRQHNEFHEQCVERIYCD
IMKFGQIDSLCVYARYTRRGGLDINPLRTTEHINEINNLRLLRQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=281, Percent_Identity=56.9395017793594, Blast_Score=321, Evalue=4e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_PSYIN (A1SYB5)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944079.1
- ProteinModelPortal:   A1SYB5
- SMR:   A1SYB5
- STRING:   A1SYB5
- GeneID:   4627058
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_2773
- eggNOG:   COG0780
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- PhylomeDB:   A1SYB5
- ProtClustDB:   PRK11792
- BioCyc:   PING357804:PING_2773-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 32849; Mature: 32717

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKPLYDQDSTLKNLSLGKITEYKSSYDPTLLQAVPRSLNRNELQLSEHNLPFYGVDLW
CCCCCCCCCCCCHHCCCCCHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEECCHH
NIYELSWLNSKGKPVVATGVVKVPFDSKNLIESKSFKLYLNSFNQSKFVSIEAVQKVLTA
HEEEEEEECCCCCEEEEEEEEEECCCCCHHHCCCCEEEEEECCCCCCEEEHHHHHHHHHH
DLSHCADKAVSVELHTDLDNFSDKLGTFSGQCLDVLDIEIDNYQLNADYLQDLSSQEQVT
HHHHHHHHHEEEEEECCHHHHHHHHCCCCCCEEEEEEEEECCEEECHHHHHCCCHHHHHH
ETLYSHLLKSNCLITSQPDWASIEISYTGKKLDREKLLRYLISFRQHNEFHEQCVERIYC
HHHHHHHHHCCCEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
DIMKFGQIDSLCVYARYTRRGGLDINPLRTTEHINEINNLRLLRQ
HHHHCCCCCCEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHEECC
>Mature Secondary Structure 
TNKPLYDQDSTLKNLSLGKITEYKSSYDPTLLQAVPRSLNRNELQLSEHNLPFYGVDLW
CCCCCCCCCCCHHCCCCCHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEECCHH
NIYELSWLNSKGKPVVATGVVKVPFDSKNLIESKSFKLYLNSFNQSKFVSIEAVQKVLTA
HEEEEEEECCCCCEEEEEEEEEECCCCCHHHCCCCEEEEEECCCCCCEEEHHHHHHHHHH
DLSHCADKAVSVELHTDLDNFSDKLGTFSGQCLDVLDIEIDNYQLNADYLQDLSSQEQVT
HHHHHHHHHEEEEEECCHHHHHHHHCCCCCCEEEEEEEEECCEEECHHHHHCCCHHHHHH
ETLYSHLLKSNCLITSQPDWASIEISYTGKKLDREKLLRYLISFRQHNEFHEQCVERIYC
HHHHHHHHHCCCEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
DIMKFGQIDSLCVYARYTRRGGLDINPLRTTEHINEINNLRLLRQ
HHHHCCCCCCEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA