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The map label for this gene is truA

Identifier: 119945656

GI number: 119945656

Start: 2394903

End: 2395688

Strand: Reverse

Name: truA

Synonym: Ping_1969

Alternate gene names: 119945656

Gene position: 2395688-2394903 (Counterclockwise)

Preceding gene: 119945657

Following gene: 119945655

Centisome position: 52.54

GC content: 44.53

Gene sequence:

>786_bases
ATGCGAATTGCACTGGGAATTGAATATGATGGTGCTGCCTATTATGGTTGGCAGCGCCAAAAAGAGGTGATCTCGGTACA
ACAGGAACTAGAACAATCGCTGTCGAAAATAGCGAATCATACGGTCAGAGTTCATTGTGCAGGACGAACAGATTCAGGTG
TGCACAGCACCGGTCAGGTGGTTCACTTTGAAACCGAAGCCATTCGTAAAGATGTTGCTTGGACACTGGGTGTCAATGCT
AATCTGCCCAAGGATATTGCAGTACGCTGGGTTAAACAGGTAGATTCATCATTTCATGCCCGTTTTAGTGCAACTGCACG
CCATTATCGTTATATTATTTATAATGGTGCCTTTCGTCCGGGCATTTTAGGGACCGGGTTGAGTCATTATCATATGGATT
TAGATGTGCAAAAAATGCATTTGGCTGGACAGATGCTCCTTGGCGAGCAAGATTTCACCTCTTTCCGTGCGTTACATTGT
CAGGCGAGCAGCCCGGTGAAATGCATTGAATATTTAAACATTAGCCAGCGCAGCCAATTCATCATTATTGATATTAAAGC
CAATGCTTTTTTACACCATATGGTCAGAAATATTGCCGGTAGCTTAATTGAGATAGGATGTGGTAAGCAACCGGTCGACT
GGCTAAAAACGTTACTAGCTTATAAAGACAGATCTAAAGCCGCGGCGACAGCAAAACCCGGCGGGTTATATTTAGTTGAA
GTTGACTATCCCTCGCAGTATGATTTACCCCGCCCCCCCTGCGGTCCGCTATTTTTAGAACTATAA

Upstream 100 bases:

>100_bases
TGCAGGAAGCTGCATTTAAGCCTTGTTAAATACAATTCATTTTTAAAAGCACAAACACATAGTATATACTATGCCATTTG
AATTTTAGGATGGATGATTA

Downstream 100 bases:

>100_bases
ATCAGAGCAAAATATGCATAATGTTATTCCTCAAAAACTTCCTCAAACACTGCCACAATGGTTGGCCTATATTGAAATAT
TACACCCAAGCGCTATCGAT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MRIALGIEYDGAAYYGWQRQKEVISVQQELEQSLSKIANHTVRVHCAGRTDSGVHSTGQVVHFETEAIRKDVAWTLGVNA
NLPKDIAVRWVKQVDSSFHARFSATARHYRYIIYNGAFRPGILGTGLSHYHMDLDVQKMHLAGQMLLGEQDFTSFRALHC
QASSPVKCIEYLNISQRSQFIIIDIKANAFLHHMVRNIAGSLIEIGCGKQPVDWLKTLLAYKDRSKAAATAKPGGLYLVE
VDYPSQYDLPRPPCGPLFLEL

Sequences:

>Translated_261_residues
MRIALGIEYDGAAYYGWQRQKEVISVQQELEQSLSKIANHTVRVHCAGRTDSGVHSTGQVVHFETEAIRKDVAWTLGVNA
NLPKDIAVRWVKQVDSSFHARFSATARHYRYIIYNGAFRPGILGTGLSHYHMDLDVQKMHLAGQMLLGEQDFTSFRALHC
QASSPVKCIEYLNISQRSQFIIIDIKANAFLHHMVRNIAGSLIEIGCGKQPVDWLKTLLAYKDRSKAAATAKPGGLYLVE
VDYPSQYDLPRPPCGPLFLEL
>Mature_261_residues
MRIALGIEYDGAAYYGWQRQKEVISVQQELEQSLSKIANHTVRVHCAGRTDSGVHSTGQVVHFETEAIRKDVAWTLGVNA
NLPKDIAVRWVKQVDSSFHARFSATARHYRYIIYNGAFRPGILGTGLSHYHMDLDVQKMHLAGQMLLGEQDFTSFRALHC
QASSPVKCIEYLNISQRSQFIIIDIKANAFLHHMVRNIAGSLIEIGCGKQPVDWLKTLLAYKDRSKAAATAKPGGLYLVE
VDYPSQYDLPRPPCGPLFLEL

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=268, Percent_Identity=27.2388059701493, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788657, Length=258, Percent_Identity=64.7286821705426, Blast_Score=369, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_PSYIN (A1SW72)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943336.1
- ProteinModelPortal:   A1SW72
- SMR:   A1SW72
- STRING:   A1SW72
- GeneID:   4627046
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1969
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   A1SW72
- BioCyc:   PING357804:PING_1969-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29299; Mature: 29299

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALGIEYDGAAYYGWQRQKEVISVQQELEQSLSKIANHTVRVHCAGRTDSGVHSTGQV
CEEEEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCE
VHFETEAIRKDVAWTLGVNANLPKDIAVRWVKQVDSSFHARFSATARHYRYIIYNGAFRP
EEEEHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCC
GILGTGLSHYHMDLDVQKMHLAGQMLLGEQDFTSFRALHCQASSPVKCIEYLNISQRSQF
CCCCCCCCCEEECCCHHHHHHHHHHHCCCCCHHHHEEEEECCCCCHHHHHHCCCCCCCCE
IIIDIKANAFLHHMVRNIAGSLIEIGCGKQPVDWLKTLLAYKDRSKAAATAKPGGLYLVE
EEEEEECHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
VDYPSQYDLPRPPCGPLFLEL
ECCCCCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MRIALGIEYDGAAYYGWQRQKEVISVQQELEQSLSKIANHTVRVHCAGRTDSGVHSTGQV
CEEEEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCE
VHFETEAIRKDVAWTLGVNANLPKDIAVRWVKQVDSSFHARFSATARHYRYIIYNGAFRP
EEEEHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCC
GILGTGLSHYHMDLDVQKMHLAGQMLLGEQDFTSFRALHCQASSPVKCIEYLNISQRSQF
CCCCCCCCCEEECCCHHHHHHHHHHHCCCCCHHHHEEEEECCCCCHHHHHHCCCCCCCCE
IIIDIKANAFLHHMVRNIAGSLIEIGCGKQPVDWLKTLLAYKDRSKAAATAKPGGLYLVE
EEEEEECHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
VDYPSQYDLPRPPCGPLFLEL
ECCCCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA