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The map label for this gene is bioB

Identifier: 119945615

GI number: 119945615

Start: 2349853

End: 2350908

Strand: Reverse

Name: bioB

Synonym: Ping_1924

Alternate gene names: 119945615

Gene position: 2350908-2349853 (Counterclockwise)

Preceding gene: 119945619

Following gene: 119945614

Centisome position: 51.56

GC content: 42.33

Gene sequence:

>1056_bases
ATGATAAATAACACAGAAATTCGCCAAAACTGGACGGTTGCAGAAGTTGATCAACTGTTTGAAATGCCGTTTATGGATCT
GGTTTTTCAAGCGCAAACGACGCACCGGGCAAACTTCCCTTTAAACGAAGTGCAGGTGAGTACTCTATTGTCGATTAAAA
CCGGGGCTTGTCCTGAAGACTGTAAATATTGCCCGCAAAGTGCACGTTATAATACCGGGCTTGCAAAAGAACAGTTAATT
GAAGTTGCCAAAGTAGTTAAGGCCGCTAAAGAGGCAAAAGCAACGGGCTCAACCCGTTTTTGTATGGGCGCTGCATGGAA
GAACCCTAAACAAAGAGATATGCCTTACTTGCTGGACATGATCAAAGAGGTGAGAGCATTAGGCCTTGAGTCATGTATGA
CGCTAGGGATGTTAGATAATAATCAAGCTGAACAGCTTTCGAATGCGGGTTTAGATTATTACAATCATAACTTAGATACC
TCACCTGAATATTATGACAAAATCATCACTACCCGGACTTATCAGGATCGCCTTGATACGCTGGAGAATGTGCGTAATTC
CGGTATGAAAGTCTGCAGCGGTGGCATTGTTGGTTTAGGGGAAGCTGGCTCAGACCGCTCCGGTTTATTGGTACAACTTG
CCAATCTGCCTCAGCAACCTGAAAGTGTGCCCATTAATAAATTAGTGAAGGTCAAAGGCACACCACTTGAAAATGCCGAT
GACCTTGATGATTTTACTTTTATTAAAACCATTGCTATCGCACGACTCTTAATGCCCAAGTCTCACGTGCGCTTATCTGC
CGGCCGTGACGATATGAATGAACAAACCCAAGCGTTATGCTTTATGGCTGGTGCCAATTCAATTTTTTACGGTTGCAAAT
TATTAACCACTGCAAATCCCGATGAAAATTCAGATACGCGTTTATTTGAAAAATTGGGAATAAACAAAGAAGCGATTAAT
TTCTCTCCCTCTGAAGCTGAAAAAAAGGTGGATGCCGCGATCGTACGTTTTAATGAAAAAGATGAGCTGTTTTTTGACGC
TACCAGTAAAGCATAA

Upstream 100 bases:

>100_bases
AACTGTTTTTATTGCCGTTTATAGACAACTGCTTTCTCAAGCTCAAATGACGCATAGAATTTAACTAACTAATTATTTTA
ACGGTAATAGAGGGAACTAA

Downstream 100 bases:

>100_bases
ACCATGGCATTTGATTTTATTACTGAGCAACTAGCTGAGCGCCATCGACAGGGGTTGTATCGCTGCTCAACCAGTATGTC
GGGTGCACAGGGCCGTTTGC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MINNTEIRQNWTVAEVDQLFEMPFMDLVFQAQTTHRANFPLNEVQVSTLLSIKTGACPEDCKYCPQSARYNTGLAKEQLI
EVAKVVKAAKEAKATGSTRFCMGAAWKNPKQRDMPYLLDMIKEVRALGLESCMTLGMLDNNQAEQLSNAGLDYYNHNLDT
SPEYYDKIITTRTYQDRLDTLENVRNSGMKVCSGGIVGLGEAGSDRSGLLVQLANLPQQPESVPINKLVKVKGTPLENAD
DLDDFTFIKTIAIARLLMPKSHVRLSAGRDDMNEQTQALCFMAGANSIFYGCKLLTTANPDENSDTRLFEKLGINKEAIN
FSPSEAEKKVDAAIVRFNEKDELFFDATSKA

Sequences:

>Translated_351_residues
MINNTEIRQNWTVAEVDQLFEMPFMDLVFQAQTTHRANFPLNEVQVSTLLSIKTGACPEDCKYCPQSARYNTGLAKEQLI
EVAKVVKAAKEAKATGSTRFCMGAAWKNPKQRDMPYLLDMIKEVRALGLESCMTLGMLDNNQAEQLSNAGLDYYNHNLDT
SPEYYDKIITTRTYQDRLDTLENVRNSGMKVCSGGIVGLGEAGSDRSGLLVQLANLPQQPESVPINKLVKVKGTPLENAD
DLDDFTFIKTIAIARLLMPKSHVRLSAGRDDMNEQTQALCFMAGANSIFYGCKLLTTANPDENSDTRLFEKLGINKEAIN
FSPSEAEKKVDAAIVRFNEKDELFFDATSKA
>Mature_351_residues
MINNTEIRQNWTVAEVDQLFEMPFMDLVFQAQTTHRANFPLNEVQVSTLLSIKTGACPEDCKYCPQSARYNTGLAKEQLI
EVAKVVKAAKEAKATGSTRFCMGAAWKNPKQRDMPYLLDMIKEVRALGLESCMTLGMLDNNQAEQLSNAGLDYYNHNLDT
SPEYYDKIITTRTYQDRLDTLENVRNSGMKVCSGGIVGLGEAGSDRSGLLVQLANLPQQPESVPINKLVKVKGTPLENAD
DLDDFTFIKTIAIARLLMPKSHVRLSAGRDDMNEQTQALCFMAGANSIFYGCKLLTTANPDENSDTRLFEKLGINKEAIN
FSPSEAEKKVDAAIVRFNEKDELFFDATSKA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=330, Percent_Identity=66.969696969697, Blast_Score=485, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6321725, Length=330, Percent_Identity=47.5757575757576, Blast_Score=322, Evalue=5e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_PSYIN (A1SW31)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943295.1
- ProteinModelPortal:   A1SW31
- SMR:   A1SW31
- STRING:   A1SW31
- GeneID:   4624995
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1924
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   A1SW31
- BioCyc:   PING357804:PING_1924-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 39037; Mature: 39037

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINNTEIRQNWTVAEVDQLFEMPFMDLVFQAQTTHRANFPLNEVQVSTLLSIKTGACPED
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCCCHHH
CKYCPQSARYNTGLAKEQLIEVAKVVKAAKEAKATGSTRFCMGAAWKNPKQRDMPYLLDM
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHH
IKEVRALGLESCMTLGMLDNNQAEQLSNAGLDYYNHNLDTSPEYYDKIITTRTYQDRLDT
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHCCCCCCCHHHHHHHHHCCHHHHHHHH
LENVRNSGMKVCSGGIVGLGEAGSDRSGLLVQLANLPQQPESVPINKLVKVKGTPLENAD
HHHHHHCCCEEECCCEEECCCCCCCCCCCEEEECCCCCCCCCCCHHHEEEECCCCCCCCC
DLDDFTFIKTIAIARLLMPKSHVRLSAGRDDMNEQTQALCFMAGANSIFYGCKLLTTANP
CCHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEECEEEEEECCC
DENSDTRLFEKLGINKEAINFSPSEAEKKVDAAIVRFNEKDELFFDATSKA
CCCCHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHEEECCCCCEEEECCCCC
>Mature Secondary Structure
MINNTEIRQNWTVAEVDQLFEMPFMDLVFQAQTTHRANFPLNEVQVSTLLSIKTGACPED
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCCCHHH
CKYCPQSARYNTGLAKEQLIEVAKVVKAAKEAKATGSTRFCMGAAWKNPKQRDMPYLLDM
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHH
IKEVRALGLESCMTLGMLDNNQAEQLSNAGLDYYNHNLDTSPEYYDKIITTRTYQDRLDT
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHCCCCCCCHHHHHHHHHCCHHHHHHHH
LENVRNSGMKVCSGGIVGLGEAGSDRSGLLVQLANLPQQPESVPINKLVKVKGTPLENAD
HHHHHHCCCEEECCCEEECCCCCCCCCCCEEEECCCCCCCCCCCHHHEEEECCCCCCCCC
DLDDFTFIKTIAIARLLMPKSHVRLSAGRDDMNEQTQALCFMAGANSIFYGCKLLTTANP
CCHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEECEEEEEECCC
DENSDTRLFEKLGINKEAINFSPSEAEKKVDAAIVRFNEKDELFFDATSKA
CCCCHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHEEECCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA