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The map label for this gene is prpB [H]

Identifier: 119945564

GI number: 119945564

Start: 2273111

End: 2273989

Strand: Reverse

Name: prpB [H]

Synonym: Ping_1869

Alternate gene names: 119945564

Gene position: 2273989-2273111 (Counterclockwise)

Preceding gene: 119945565

Following gene: 119945563

Centisome position: 49.87

GC content: 45.51

Gene sequence:

>879_bases
ATGACGCCAGGTAAAAAATTCAGACAAGCACTTATCAATAATAAACCGCTGCAGATAGTTGGCACCATCAATCCTTACTG
CGCCATGATGGCAGAGCAACTTGGTCATCAGGCCATTTATCTGTCTGGTGGAGGTGTCGCCAATGCTTCTTATGGTTTAC
CTGATTTGGGCATGACCTCATTAAACGATGTTATTGCTGATGTGCAGCGTATTACATCCGCATCTGCTTTACCTTTACTG
GTTGATATCGATACCGGATGGGGAGGCGCTTTTAATATTGCTAAAACTGTTCGCGATATGGAAAAAGCCGGCGCGGCAGC
TGTACATATTGAAGATCAAGTGTCCCAAAAACGTTGCGGTCACCGCCCTAATAAAGAGATTGTTTCCACACAGGAAATGG
CAGATCGCATCAAGGCTGCAGTGGATGCACGTATCGACAGCGACTTTTTTATTATGGCACGCACCGACTCCTTCGCCCAG
GAAGGCTTAGAATCTGCCATTGCACGCGCACAAGCCTATGTTGCAGCCGGCGCTGATGGTATTTTTGCTGAAGCCATCAA
AACGGAAGCGCACTACCGTGCATTTTCGAGAGCCTTGAATGTGCCTATTCTTGCCAATATCACAGAATTTGGTGAAACCG
AATTATGGAATAAAAAACAACTCGGCGAATGGGGCTGCGCAATGGTGCTCTATCCTCTTTCCGCTTTCAGAGCCATGAAT
AAAGCCGCTGAATCAGTTTATAAAACCATACTTGTCGATGGTGATCAAAAAGCAGTGGTCGATACGATGCAGACACGAAT
GGAGCTATACAAATACCTCGGCTACCACGATTACGAGCAAAAACTTGACGAATTATTTGCCGATCAAAAAAACAAGTAA

Upstream 100 bases:

>100_bases
CTTATCAATAATAAACCGCTGTTGAAATGTTAATGCGCTGGCATGTCAAACGCAGTCCGGAATTAATCGAAAACCAACTG
ATAGAAGAAGGAAAAAACTA

Downstream 100 bases:

>100_bases
CGTTGAGTCATCTTGACAATAAATTCAATTAAAAATTTAATAAATATAAGGAAAGTATATGAGCAATAAAAAAGCGGGCG
GTGCAGGTCTTCGTGGACAA

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTPGKKFRQALINNKPLQIVGTINPYCAMMAEQLGHQAIYLSGGGVANASYGLPDLGMTSLNDVIADVQRITSASALPLL
VDIDTGWGGAFNIAKTVRDMEKAGAAAVHIEDQVSQKRCGHRPNKEIVSTQEMADRIKAAVDARIDSDFFIMARTDSFAQ
EGLESAIARAQAYVAAGADGIFAEAIKTEAHYRAFSRALNVPILANITEFGETELWNKKQLGEWGCAMVLYPLSAFRAMN
KAAESVYKTILVDGDQKAVVDTMQTRMELYKYLGYHDYEQKLDELFADQKNK

Sequences:

>Translated_292_residues
MTPGKKFRQALINNKPLQIVGTINPYCAMMAEQLGHQAIYLSGGGVANASYGLPDLGMTSLNDVIADVQRITSASALPLL
VDIDTGWGGAFNIAKTVRDMEKAGAAAVHIEDQVSQKRCGHRPNKEIVSTQEMADRIKAAVDARIDSDFFIMARTDSFAQ
EGLESAIARAQAYVAAGADGIFAEAIKTEAHYRAFSRALNVPILANITEFGETELWNKKQLGEWGCAMVLYPLSAFRAMN
KAAESVYKTILVDGDQKAVVDTMQTRMELYKYLGYHDYEQKLDELFADQKNK
>Mature_291_residues
TPGKKFRQALINNKPLQIVGTINPYCAMMAEQLGHQAIYLSGGGVANASYGLPDLGMTSLNDVIADVQRITSASALPLLV
DIDTGWGGAFNIAKTVRDMEKAGAAAVHIEDQVSQKRCGHRPNKEIVSTQEMADRIKAAVDARIDSDFFIMARTDSFAQE
GLESAIARAQAYVAAGADGIFAEAIKTEAHYRAFSRALNVPILANITEFGETELWNKKQLGEWGCAMVLYPLSAFRAMNK
AAESVYKTILVDGDQKAVVDTMQTRMELYKYLGYHDYEQKLDELFADQKNK

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=292, Percent_Identity=61.986301369863, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1790445, Length=239, Percent_Identity=30.1255230125523, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32054; Mature: 31923

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPGKKFRQALINNKPLQIVGTINPYCAMMAEQLGHQAIYLSGGGVANASYGLPDLGMTS
CCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCHHH
LNDVIADVQRITSASALPLLVDIDTGWGGAFNIAKTVRDMEKAGAAAVHIEDQVSQKRCG
HHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCC
HRPNKEIVSTQEMADRIKAAVDARIDSDFFIMARTDSFAQEGLESAIARAQAYVAAGADG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCH
IFAEAIKTEAHYRAFSRALNVPILANITEFGETELWNKKQLGEWGCAMVLYPLSAFRAMN
HHHHHHHHHHHHHHHHHHCCCCCEECHHHHCCHHCCCHHHCCHHHHHHHHHHHHHHHHHH
KAAESVYKTILVDGDQKAVVDTMQTRMELYKYLGYHDYEQKLDELFADQKNK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TPGKKFRQALINNKPLQIVGTINPYCAMMAEQLGHQAIYLSGGGVANASYGLPDLGMTS
CCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCHHH
LNDVIADVQRITSASALPLLVDIDTGWGGAFNIAKTVRDMEKAGAAAVHIEDQVSQKRCG
HHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCC
HRPNKEIVSTQEMADRIKAAVDARIDSDFFIMARTDSFAQEGLESAIARAQAYVAAGADG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCH
IFAEAIKTEAHYRAFSRALNVPILANITEFGETELWNKKQLGEWGCAMVLYPLSAFRAMN
HHHHHHHHHHHHHHHHHHCCCCCEECHHHHCCHHCCCHHHCCHHHHHHHHHHHHHHHHHH
KAAESVYKTILVDGDQKAVVDTMQTRMELYKYLGYHDYEQKLDELFADQKNK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]