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The map label for this gene is ycdT [H]

Identifier: 119945517

GI number: 119945517

Start: 2209599

End: 2210495

Strand: Reverse

Name: ycdT [H]

Synonym: Ping_1821

Alternate gene names: 119945517

Gene position: 2210495-2209599 (Counterclockwise)

Preceding gene: 119945518

Following gene: 119945516

Centisome position: 48.48

GC content: 34.78

Gene sequence:

>897_bases
ATGAAAAAAAACGCAGTTCCAAATTTAAACGATTTTTTTTCTGGTCATCTTATTGTAAATAAAGCTAGAAAAATTCTTTA
TTGCAATAATTATATTTGTGATTTAAGTGACCAGTCTGATAAAAGTCTGATTGATAACCCCCTATCCAAATTTGTTACCA
AAGCATCTAATATTTTTATTGATAGTTATGTTTATCCACTATTAATGCATCAATCATGCGCACAAGAAATCCAAATAACG
TGGATTGGGAAAAACGGAGAAACAATACCCGTTGTAGTGAATATAAAGCTGGGGTCAGAAGATATATCTTACTGGTCACT
TTACGTCTGTGAAAATCGAGATAAGCTTCACAGTGAGCTGTTAAAAGCGAATGAAAAGCTTGAAAAACAGTCTCAAGAGT
TATTTCGTCTGGCAACAACAGATCCCCTGACCGGTTTATTGAACAGGCGCGAACTGCAAGTACAGGCCCAAAAAGTCACT
CATCAAGCTGCCAGAAACTTATCAACATTTGCATTATTGTTTATTGATATTGATTTTTTTAAACGTGTTAATGATACATA
CGGCCATCCCGCTGGAGATAAGGTTTTAATATGCCTGGCTAATATACTGACTCAAGAACGAAGAGTAACTGATATAGTGG
CAAGAATGGGAGGTGAAGAATTTGCATTACTATTACCCAATATTGATGAAGAAAATGCCTATAAATTAGCCGAAAGATTA
AGGAAAAAAATTGAAAAGCAAACAATAGACCATATAAAAATCACCGTAAGTATCGGCTTAGTCGTCACGCGTAAAAACAC
CCAAACAAATTTTGATATATTACTTGAATTAGCTGATCAAGCACTTTATGAGTCCAAAGAATGGGGACGAAACAGAACGC
ATGTTGCTCAACTTTAA

Upstream 100 bases:

>100_bases
AATGAACTTGCCATTATTAATGCAGAAGGCCATTGTTTACATATGACAAATCACCAAGATATTATCCCGATAATTCTTCG
TTTTATTGGACGATAGCCGA

Downstream 100 bases:

>100_bases
AAGACAGTTTACGAGAAATACAGTGAAATTCGGCTATATCTATGGCCTTCCACATTAGTCAAATCATGGAATAACATCAA
TCGTTAAAATACTATGTTGA

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKKNAVPNLNDFFSGHLIVNKARKILYCNNYICDLSDQSDKSLIDNPLSKFVTKASNIFIDSYVYPLLMHQSCAQEIQIT
WIGKNGETIPVVVNIKLGSEDISYWSLYVCENRDKLHSELLKANEKLEKQSQELFRLATTDPLTGLLNRRELQVQAQKVT
HQAARNLSTFALLFIDIDFFKRVNDTYGHPAGDKVLICLANILTQERRVTDIVARMGGEEFALLLPNIDEENAYKLAERL
RKKIEKQTIDHIKITVSIGLVVTRKNTQTNFDILLELADQALYESKEWGRNRTHVAQL

Sequences:

>Translated_298_residues
MKKNAVPNLNDFFSGHLIVNKARKILYCNNYICDLSDQSDKSLIDNPLSKFVTKASNIFIDSYVYPLLMHQSCAQEIQIT
WIGKNGETIPVVVNIKLGSEDISYWSLYVCENRDKLHSELLKANEKLEKQSQELFRLATTDPLTGLLNRRELQVQAQKVT
HQAARNLSTFALLFIDIDFFKRVNDTYGHPAGDKVLICLANILTQERRVTDIVARMGGEEFALLLPNIDEENAYKLAERL
RKKIEKQTIDHIKITVSIGLVVTRKNTQTNFDILLELADQALYESKEWGRNRTHVAQL
>Mature_298_residues
MKKNAVPNLNDFFSGHLIVNKARKILYCNNYICDLSDQSDKSLIDNPLSKFVTKASNIFIDSYVYPLLMHQSCAQEIQIT
WIGKNGETIPVVVNIKLGSEDISYWSLYVCENRDKLHSELLKANEKLEKQSQELFRLATTDPLTGLLNRRELQVQAQKVT
HQAARNLSTFALLFIDIDFFKRVNDTYGHPAGDKVLICLANILTQERRVTDIVARMGGEEFALLLPNIDEENAYKLAERL
RKKIEKQTIDHIKITVSIGLVVTRKNTQTNFDILLELADQALYESKEWGRNRTHVAQL

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=169, Percent_Identity=44.3786982248521, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI87081881, Length=173, Percent_Identity=41.0404624277457, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI87082007, Length=160, Percent_Identity=36.25, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693134, Length=160, Percent_Identity=35.625, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=33.5260115606936, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787802, Length=129, Percent_Identity=41.0852713178295, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=39.1304347826087, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=34.5454545454545, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788381, Length=174, Percent_Identity=33.9080459770115, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788956, Length=168, Percent_Identity=33.3333333333333, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=30.9941520467836, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081977, Length=178, Percent_Identity=30.8988764044944, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87081974, Length=165, Percent_Identity=29.6969696969697, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787056, Length=163, Percent_Identity=30.6748466257669, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34125; Mature: 34125

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNAVPNLNDFFSGHLIVNKARKILYCNNYICDLSDQSDKSLIDNPLSKFVTKASNIFI
CCCCCCCCHHHHHCCCEEEECCCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHEEH
DSYVYPLLMHQSCAQEIQITWIGKNGETIPVVVNIKLGSEDISYWSLYVCENRDKLHSEL
HHHHHHHHHHHHHHCEEEEEEECCCCCEEEEEEEEEECCCCCCEEEEEEECCHHHHHHHH
LKANEKLEKQSQELFRLATTDPLTGLLNRRELQVQAQKVTHQAARNLSTFALLFIDIDFF
HHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHCHHHEEEEEEEHHHH
KRVNDTYGHPAGDKVLICLANILTQERRVTDIVARMGGEEFALLLPNIDEENAYKLAERL
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHH
RKKIEKQTIDHIKITVSIGLVVTRKNTQTNFDILLELADQALYESKEWGRNRTHVAQL
HHHHHHHHHCEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCC
>Mature Secondary Structure
MKKNAVPNLNDFFSGHLIVNKARKILYCNNYICDLSDQSDKSLIDNPLSKFVTKASNIFI
CCCCCCCCHHHHHCCCEEEECCCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHEEH
DSYVYPLLMHQSCAQEIQITWIGKNGETIPVVVNIKLGSEDISYWSLYVCENRDKLHSEL
HHHHHHHHHHHHHHCEEEEEEECCCCCEEEEEEEEEECCCCCCEEEEEEECCHHHHHHHH
LKANEKLEKQSQELFRLATTDPLTGLLNRRELQVQAQKVTHQAARNLSTFALLFIDIDFF
HHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHCHHHEEEEEEEHHHH
KRVNDTYGHPAGDKVLICLANILTQERRVTDIVARMGGEEFALLLPNIDEENAYKLAERL
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHH
RKKIEKQTIDHIKITVSIGLVVTRKNTQTNFDILLELADQALYESKEWGRNRTHVAQL
HHHHHHHHHCEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]