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The map label for this gene is hisF

Identifier: 119945359

GI number: 119945359

Start: 2004129

End: 2004902

Strand: Reverse

Name: hisF

Synonym: Ping_1647

Alternate gene names: 119945359

Gene position: 2004902-2004129 (Counterclockwise)

Preceding gene: 119945361

Following gene: 119945358

Centisome position: 43.97

GC content: 44.83

Gene sequence:

>774_bases
ATGTTAGCAAAGCGCATAATTCCCTGTCTTGATGTCAAGGACGGGCAGGTCGTAAAAGGCGTGCAATTTCGTAATCACGA
AATTGTCGGTGATATTGTCCCTCTCGCTAAACGTTACGCAGAAGAAGGCGCCGATGAATTAGTCTTTTACGATATAACTG
CCAGCTCAGATGCACGCGTCGTTGATAAAAGCTGGGTAAGCCGTGTCGCAGAAGTGATTGATATCCCTTTCTGCGTGGCC
GGTGGTATCAAGACTATCGATGACGCTCGACGTATTTTAGAGTTTGGCGCAGATAAAGTGTCCATTAACAGCCCGGCGCT
TGCAGATCCCAGTTTGATTAATCGCCTTTCTGAAAAATTCGGCGTGCAGTGTATTGTTATCGGTATTGATTCCTACTTTG
ATAAGGAAACGGGTTTATACCAGGTTAAGCAGTTTACCGGTAATGAAAAGGCAACCTTGACCACCAAATGGAATACTTTT
GATTGGATTAAAGAAGTACAGTCTCGTGGGGCAGGTGAAATCGTGCTTAATGTGATGAACCAGGATGGCGTTCGTTGCGG
TTACGATATCGATCAACTCACTCAGGCACGTGCAGTTTGTAATGTACCGTTAATTGCTTCTGGTGGTGCCGGTGAAATGG
TCCATTTCGCCGAAGTCTTTAAGCAGGCAGATGTTGATGGTGCTCTGGCTGCGAGTGTATTCCACAAGCAGATAATTAAT
ATTGGTGAGTTGAAAGCTTACCTTAAAACACAGCAGGTAGAGATAAGATTATGA

Upstream 100 bases:

>100_bases
CTAATATTTTTGTATGCAAATATGATGCTACTTAAATTACACAGAAAAAAATATTGGATAACACTTTGAAATATATAAAA
TTACTACATAAGAGGTCATC

Downstream 100 bases:

>100_bases
ACAAAGCGTTAATGAATGAACAACAGCTTACAGCATTAGATTGGGAAAAAGTTGATAACTTGATGCCGGTTATTGTGCAA
AATCGCACCTCAGGCAAAGT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLAKRIIPCLDVKDGQVVKGVQFRNHEIVGDIVPLAKRYAEEGADELVFYDITASSDARVVDKSWVSRVAEVIDIPFCVA
GGIKTIDDARRILEFGADKVSINSPALADPSLINRLSEKFGVQCIVIGIDSYFDKETGLYQVKQFTGNEKATLTTKWNTF
DWIKEVQSRGAGEIVLNVMNQDGVRCGYDIDQLTQARAVCNVPLIASGGAGEMVHFAEVFKQADVDGALAASVFHKQIIN
IGELKAYLKTQQVEIRL

Sequences:

>Translated_257_residues
MLAKRIIPCLDVKDGQVVKGVQFRNHEIVGDIVPLAKRYAEEGADELVFYDITASSDARVVDKSWVSRVAEVIDIPFCVA
GGIKTIDDARRILEFGADKVSINSPALADPSLINRLSEKFGVQCIVIGIDSYFDKETGLYQVKQFTGNEKATLTTKWNTF
DWIKEVQSRGAGEIVLNVMNQDGVRCGYDIDQLTQARAVCNVPLIASGGAGEMVHFAEVFKQADVDGALAASVFHKQIIN
IGELKAYLKTQQVEIRL
>Mature_257_residues
MLAKRIIPCLDVKDGQVVKGVQFRNHEIVGDIVPLAKRYAEEGADELVFYDITASSDARVVDKSWVSRVAEVIDIPFCVA
GGIKTIDDARRILEFGADKVSINSPALADPSLINRLSEKFGVQCIVIGIDSYFDKETGLYQVKQFTGNEKATLTTKWNTF
DWIKEVQSRGAGEIVLNVMNQDGVRCGYDIDQLTQARAVCNVPLIASGGAGEMVHFAEVFKQADVDGALAASVFHKQIIN
IGELKAYLKTQQVEIRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=79.3774319066148, Blast_Score=433, Evalue=1e-123,
Organism=Escherichia coli, GI87082028, Length=215, Percent_Identity=24.6511627906977, Blast_Score=64, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=28.343949044586, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_PSYIN (A1SVC5)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943039.1
- ProteinModelPortal:   A1SVC5
- SMR:   A1SVC5
- STRING:   A1SVC5
- GeneID:   4623622
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1647
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   ASVFHKG
- PhylomeDB:   A1SVC5
- ProtClustDB:   PRK02083
- BioCyc:   PING357804:PING_1647-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 28268; Mature: 28268

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVKDGQVVKGVQFRNHEIVGDIVPLAKRYAEEGADELVFYDITASSDARV
CCHHHCCCCCCCCCCCEEECEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHH
VDKSWVSRVAEVIDIPFCVAGGIKTIDDARRILEFGADKVSINSPALADPSLINRLSEKF
HHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVIGIDSYFDKETGLYQVKQFTGNEKATLTTKWNTFDWIKEVQSRGAGEIVLNVMN
CCEEEEEECCCCCCCCCCCEEEEECCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEEEEC
QDGVRCGYDIDQLTQARAVCNVPLIASGGAGEMVHFAEVFKQADVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IGELKAYLKTQQVEIRL
HHHHHHHHHHCEEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVKDGQVVKGVQFRNHEIVGDIVPLAKRYAEEGADELVFYDITASSDARV
CCHHHCCCCCCCCCCCEEECEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHH
VDKSWVSRVAEVIDIPFCVAGGIKTIDDARRILEFGADKVSINSPALADPSLINRLSEKF
HHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVIGIDSYFDKETGLYQVKQFTGNEKATLTTKWNTFDWIKEVQSRGAGEIVLNVMN
CCEEEEEECCCCCCCCCCCEEEEECCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEEEEC
QDGVRCGYDIDQLTQARAVCNVPLIASGGAGEMVHFAEVFKQADVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IGELKAYLKTQQVEIRL
HHHHHHHHHHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA