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The map label for this gene is hisS [H]

Identifier: 119944924

GI number: 119944924

Start: 1459726

End: 1461072

Strand: Direct

Name: hisS [H]

Synonym: Ping_1169

Alternate gene names: 119944924

Gene position: 1459726-1461072 (Clockwise)

Preceding gene: 119944923

Following gene: 119944925

Centisome position: 32.01

GC content: 41.5

Gene sequence:

>1347_bases
ATGGTTTCATCTAATTTGAAAAGTCCCTATAATGACTCACTCTTTTATTCTCAACTGTATGTGGAAATCATTGTGGCAAA
AAAGATTCAAGCAATTCGTGGCATGAACGATACCCTTCCTGAACAAACACCCGTTTGGCAAAAATTAGAGTCATTATTCA
GGCAAGTCGTGAGCAGTTACGGTTACTCTGAAATACGTATGCCAATTGTTGAAAATACGACTCTTTTCAAACGTTCAATC
GGCGAAGTGACCGATATTGTTGAAAAAGAGATGTATACTTTTGCCGATCGTAACGGAGATAGCTTAACATTACGTCCTGA
GGGAACCGCATCCTGCGTGCGTGCCGGAAACGAGCATGGCTTATTATATAATCAGGAACGCCGTTTATGGTACACCGGCC
CGATGTTTAGACACGAACGCCCACAAAAAGGACGCTACCGTCAGTTTCACCAATTTGGTGTGGAGACCTTCGGTATAGGC
AGTGCAGATATTGACGCTGAAGTAATTTTACTTTCAGCGCGTTTATGGCGAGCGCTTGGGATAGATAAACAGGTAGAATT
ACAGTTGAACTCTTTAGGCTCTAACGAAGCGCGCGCGTTATACAAAGAAGCATTAGTTGTCTATTTGTCGACCCATGTTG
AACAGCTGGATGAGGATAGCAAGCGTCGTATGGTAAGTAATCCATTACGTGTTCTTGATAGTAAAAATCCGGATATTCAA
GCGTTATTAGTCGATGCACCAAAACTATCCGCCTATCTTGATGACGAATCTGCCGAACACTTTGCCCAACTGCAAAAATA
TCTTGATGCAGCGGGTATTAAATATGTTATTAATGAGCGTTTAGTACGTGGTTTAGATTATTATAACCGTACTGTCTTTG
AGTGGGTAACCACTAGCCTTGGATCGCAAGGTACAATCTGTGCGGGTGGTCGTTACGATGGATTGGTTGAACAACTCGGT
GGTAAAGCAACGCCTGCGGTTGGCTTTGCAATGGGTCTGGAAAGAATTATTCTGATGCTGGAAACACTGGCATTAAATGA
TGATGTTAAAAAAGATGTTGATATATATAGTATAGTTGTTGGTGACGGTGCAGATATAGCCGGTTTTAAGGTGGCAGAAC
TGATTCGAACTGAATTGCCGATGCTTAAAATGATGCACCATTGCGGTGCTGGTAACTTTAAAAAACAGATGAAACGCGCT
GATAAAAGTGGTGCACGTTTGGCACTGATCATTGCTGAAAATGAGTTAGAGAGCGATTGTGTCACAATAAAAGACTTGCA
AGGTAAGTATGAGCAACAAAGTGTTGCAATGAATGAGCTAATTAAGGTATTACCTAGCTATTTTTAA

Upstream 100 bases:

>100_bases
GTATCATGGGCATAACTCAGCCTGTTATTTTTAAGGAATAACAGGATTTTTCATAAGTTAGTCAATAAGATATTAAAAAT
TGTCTGAGAAAGCCCGCTGC

Downstream 100 bases:

>100_bases
CATTATCTTTAAAAATCTATAACAAGGAATAAACAGTGGTTGATATTATCGAAGGTTACGAAACAGAAGAACAGCAGGTT
ATTGCGATTAAAAAATGGTG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MVSSNLKSPYNDSLFYSQLYVEIIVAKKIQAIRGMNDTLPEQTPVWQKLESLFRQVVSSYGYSEIRMPIVENTTLFKRSI
GEVTDIVEKEMYTFADRNGDSLTLRPEGTASCVRAGNEHGLLYNQERRLWYTGPMFRHERPQKGRYRQFHQFGVETFGIG
SADIDAEVILLSARLWRALGIDKQVELQLNSLGSNEARALYKEALVVYLSTHVEQLDEDSKRRMVSNPLRVLDSKNPDIQ
ALLVDAPKLSAYLDDESAEHFAQLQKYLDAAGIKYVINERLVRGLDYYNRTVFEWVTTSLGSQGTICAGGRYDGLVEQLG
GKATPAVGFAMGLERIILMLETLALNDDVKKDVDIYSIVVGDGADIAGFKVAELIRTELPMLKMMHHCGAGNFKKQMKRA
DKSGARLALIIAENELESDCVTIKDLQGKYEQQSVAMNELIKVLPSYF

Sequences:

>Translated_448_residues
MVSSNLKSPYNDSLFYSQLYVEIIVAKKIQAIRGMNDTLPEQTPVWQKLESLFRQVVSSYGYSEIRMPIVENTTLFKRSI
GEVTDIVEKEMYTFADRNGDSLTLRPEGTASCVRAGNEHGLLYNQERRLWYTGPMFRHERPQKGRYRQFHQFGVETFGIG
SADIDAEVILLSARLWRALGIDKQVELQLNSLGSNEARALYKEALVVYLSTHVEQLDEDSKRRMVSNPLRVLDSKNPDIQ
ALLVDAPKLSAYLDDESAEHFAQLQKYLDAAGIKYVINERLVRGLDYYNRTVFEWVTTSLGSQGTICAGGRYDGLVEQLG
GKATPAVGFAMGLERIILMLETLALNDDVKKDVDIYSIVVGDGADIAGFKVAELIRTELPMLKMMHHCGAGNFKKQMKRA
DKSGARLALIIAENELESDCVTIKDLQGKYEQQSVAMNELIKVLPSYF
>Mature_448_residues
MVSSNLKSPYNDSLFYSQLYVEIIVAKKIQAIRGMNDTLPEQTPVWQKLESLFRQVVSSYGYSEIRMPIVENTTLFKRSI
GEVTDIVEKEMYTFADRNGDSLTLRPEGTASCVRAGNEHGLLYNQERRLWYTGPMFRHERPQKGRYRQFHQFGVETFGIG
SADIDAEVILLSARLWRALGIDKQVELQLNSLGSNEARALYKEALVVYLSTHVEQLDEDSKRRMVSNPLRVLDSKNPDIQ
ALLVDAPKLSAYLDDESAEHFAQLQKYLDAAGIKYVINERLVRGLDYYNRTVFEWVTTSLGSQGTICAGGRYDGLVEQLG
GKATPAVGFAMGLERIILMLETLALNDDVKKDVDIYSIVVGDGADIAGFKVAELIRTELPMLKMMHHCGAGNFKKQMKRA
DKSGARLALIIAENELESDCVTIKDLQGKYEQQSVAMNELIKVLPSYF

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=418, Percent_Identity=66.5071770334928, Blast_Score=581, Evalue=1e-167,
Organism=Saccharomyces cerevisiae, GI6325290, Length=448, Percent_Identity=24.3303571428571, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24643061, Length=402, Percent_Identity=25.6218905472637, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24643059, Length=417, Percent_Identity=25.6594724220624, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 50465; Mature: 50465

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSSNLKSPYNDSLFYSQLYVEIIVAKKIQAIRGMNDTLPEQTPVWQKLESLFRQVVSSY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHC
GYSEIRMPIVENTTLFKRSIGEVTDIVEKEMYTFADRNGDSLTLRPEGTASCVRAGNEHG
CCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCCCC
LLYNQERRLWYTGPMFRHERPQKGRYRQFHQFGVETFGIGSADIDAEVILLSARLWRALG
CEEECCCCEEEECCCCCCCCCCCCHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHC
IDKQVELQLNSLGSNEARALYKEALVVYLSTHVEQLDEDSKRRMVSNPLRVLDSKNPDIQ
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEE
ALLVDAPKLSAYLDDESAEHFAQLQKYLDAAGIKYVINERLVRGLDYYNRTVFEWVTTSL
EEEECCCCHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSQGTICAGGRYDGLVEQLGGKATPAVGFAMGLERIILMLETLALNDDVKKDVDIYSIVV
CCCCCEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCEEEEEE
GDGADIAGFKVAELIRTELPMLKMMHHCGAGNFKKQMKRADKSGARLALIIAENELESDC
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCC
VTIKDLQGKYEQQSVAMNELIKVLPSYF
EEHHHHCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVSSNLKSPYNDSLFYSQLYVEIIVAKKIQAIRGMNDTLPEQTPVWQKLESLFRQVVSSY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHC
GYSEIRMPIVENTTLFKRSIGEVTDIVEKEMYTFADRNGDSLTLRPEGTASCVRAGNEHG
CCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCCCC
LLYNQERRLWYTGPMFRHERPQKGRYRQFHQFGVETFGIGSADIDAEVILLSARLWRALG
CEEECCCCEEEECCCCCCCCCCCCHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHC
IDKQVELQLNSLGSNEARALYKEALVVYLSTHVEQLDEDSKRRMVSNPLRVLDSKNPDIQ
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEE
ALLVDAPKLSAYLDDESAEHFAQLQKYLDAAGIKYVINERLVRGLDYYNRTVFEWVTTSL
EEEECCCCHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSQGTICAGGRYDGLVEQLGGKATPAVGFAMGLERIILMLETLALNDDVKKDVDIYSIVV
CCCCCEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCEEEEEE
GDGADIAGFKVAELIRTELPMLKMMHHCGAGNFKKQMKRADKSGARLALIIAENELESDC
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCC
VTIKDLQGKYEQQSVAMNELIKVLPSYF
EEHHHHCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]