Click here to switch to the map view.

The map label for this gene is ispG

Identifier: 119944923

GI number: 119944923

Start: 1458139

End: 1459248

Strand: Direct

Name: ispG

Synonym: Ping_1168

Alternate gene names: 119944923

Gene position: 1458139-1459248 (Clockwise)

Preceding gene: 119944922

Following gene: 119944924

Centisome position: 31.98

GC content: 43.69

Gene sequence:

>1110_bases
ATGAGCCATCCTTCTTCAATTGTACGTCGTGTATCAAAACAGATCATGGTGGGTAATGTACCTGTTGGTGGAAATGCGCC
AATTACAGTGCAATCTATGACCAATACTTTGACCACGGACGTTGCTGCAACAGTAGCACAAATCAAACGCTTAGAAGCGG
TTGGTGCGGATATGGTGCGCGTTTCAGTGCCGACTATGAATGCTGCGGAGGCTTTTCGAGAAATTAAAGCCTTAAGTAAT
ATCCCGCTGATTGCCGATATCCATTTTGATTATCGTATCGCCCTGAAAGTCGCTGAATATGGCGTTGATTGTCTGCGCAT
TAACCCGGGAAATATTGGTAATGAGCGACGCATCCGCAGCGTTGTTGATTGTGCGCGTGATCATAATATTCCTATTCGAA
TAGGGGTAAATGGCGGCTCATTGGAAGCTGATATTCAAGCAAAATATGGGGAGCCAACGGCACAAGCATTGGTTGATTCT
GCATTACGCCATGTGGATATTTTAGACCGGCTTAATTTTGATCAATTTAAAGTGAGTGTAAAAGCATCAGATGCACAGCT
GGCGGTTGAATCATATCAACTGCTTGCAAAACAGATAAACCAACCTCTGCATTTAGGGATCACCGAAGCCGGTGGACTTC
GCAGTGGCTCGGTTAAATCAGCGGTTGGCTTAGGTATGTTACTTTCCCAAGGCATAGGCGATACCCTACGAGTATCCTTG
GCTGCAGATCCTGTAGAGGAAATTAAAGTGGGTTTTGATATTTTAAAATCATTACGTATTCGCAGCCGTGGTATTAATTT
TATTGCCTGTCCAACCTGTTCACGTCAAGAATTTGATGTTATTGCGACGGTTAATGCTTTAGAGGAACGTCTTGAAGATA
TTACTATGCCAATGGATGTCTCGATTATTGGTTGTGTCGTCAATGGGCCGGGAGAAGCGTTAATGTCTGATTTGGGCATT
ACTGGCGGTAGCAATAAAAGTGGTTTTTACGAAGATGGGGTGCGTCAACGGGATCGTTTTGATAATAATAACGTGATTGA
TCAGCTTGAAGCGAAAATTCGAGCCCGTGTCAGTCGTACGGATCAGAGCAATATCATCCTAAAAGTTTAA

Upstream 100 bases:

>100_bases
GAAAGCGAATAGAGCATCTTAATGCGGTTTGAGGGGAAATATTATTACCAAATGGACTATTCTTTGAAACAGTGGTCTAT
CAGCAAAGAGTAAAAGTATT

Downstream 100 bases:

>100_bases
ATCATTAACGCTATACCAAACCACTTAAAGATGCAGTGTCCTGCATTTTTAAGTAATTTGGGTATCTATACCCATGTTAC
TTCAAGATGCTTTGTCAGGC

Product: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MSHPSSIVRRVSKQIMVGNVPVGGNAPITVQSMTNTLTTDVAATVAQIKRLEAVGADMVRVSVPTMNAAEAFREIKALSN
IPLIADIHFDYRIALKVAEYGVDCLRINPGNIGNERRIRSVVDCARDHNIPIRIGVNGGSLEADIQAKYGEPTAQALVDS
ALRHVDILDRLNFDQFKVSVKASDAQLAVESYQLLAKQINQPLHLGITEAGGLRSGSVKSAVGLGMLLSQGIGDTLRVSL
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIATVNALEERLEDITMPMDVSIIGCVVNGPGEALMSDLGI
TGGSNKSGFYEDGVRQRDRFDNNNVIDQLEAKIRARVSRTDQSNIILKV

Sequences:

>Translated_369_residues
MSHPSSIVRRVSKQIMVGNVPVGGNAPITVQSMTNTLTTDVAATVAQIKRLEAVGADMVRVSVPTMNAAEAFREIKALSN
IPLIADIHFDYRIALKVAEYGVDCLRINPGNIGNERRIRSVVDCARDHNIPIRIGVNGGSLEADIQAKYGEPTAQALVDS
ALRHVDILDRLNFDQFKVSVKASDAQLAVESYQLLAKQINQPLHLGITEAGGLRSGSVKSAVGLGMLLSQGIGDTLRVSL
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIATVNALEERLEDITMPMDVSIIGCVVNGPGEALMSDLGI
TGGSNKSGFYEDGVRQRDRFDNNNVIDQLEAKIRARVSRTDQSNIILKV
>Mature_368_residues
SHPSSIVRRVSKQIMVGNVPVGGNAPITVQSMTNTLTTDVAATVAQIKRLEAVGADMVRVSVPTMNAAEAFREIKALSNI
PLIADIHFDYRIALKVAEYGVDCLRINPGNIGNERRIRSVVDCARDHNIPIRIGVNGGSLEADIQAKYGEPTAQALVDSA
LRHVDILDRLNFDQFKVSVKASDAQLAVESYQLLAKQINQPLHLGITEAGGLRSGSVKSAVGLGMLLSQGIGDTLRVSLA
ADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIATVNALEERLEDITMPMDVSIIGCVVNGPGEALMSDLGIT
GGSNKSGFYEDGVRQRDRFDNNNVIDQLEAKIRARVSRTDQSNIILKV

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=363, Percent_Identity=80.4407713498623, Blast_Score=593, Evalue=1e-171,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_PSYIN (A1SU39)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942603.1
- STRING:   A1SU39
- GeneID:   4624557
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1168
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- PhylomeDB:   A1SU39
- BioCyc:   PING357804:PING_1168-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 39899; Mature: 39768

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHPSSIVRRVSKQIMVGNVPVGGNAPITVQSMTNTLTTDVAATVAQIKRLEAVGADMVR
CCCHHHHHHHHHHHHEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VSVPTMNAAEAFREIKALSNIPLIADIHFDYRIALKVAEYGVDCLRINPGNIGNERRIRS
EECCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCCHHHHHH
VVDCARDHNIPIRIGVNGGSLEADIQAKYGEPTAQALVDSALRHVDILDRLNFDQFKVSV
HHHHHHCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
KASDAQLAVESYQLLAKQINQPLHLGITEAGGLRSGSVKSAVGLGMLLSQGIGDTLRVSL
ECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIATVNALEERLEDITMPMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCE
SIIGCVVNGPGEALMSDLGITGGSNKSGFYEDGVRQRDRFDNNNVIDQLEAKIRARVSRT
EEEEEEECCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
DQSNIILKV
CCCCEEEEC
>Mature Secondary Structure 
SHPSSIVRRVSKQIMVGNVPVGGNAPITVQSMTNTLTTDVAATVAQIKRLEAVGADMVR
CCHHHHHHHHHHHHEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VSVPTMNAAEAFREIKALSNIPLIADIHFDYRIALKVAEYGVDCLRINPGNIGNERRIRS
EECCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCCHHHHHH
VVDCARDHNIPIRIGVNGGSLEADIQAKYGEPTAQALVDSALRHVDILDRLNFDQFKVSV
HHHHHHCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
KASDAQLAVESYQLLAKQINQPLHLGITEAGGLRSGSVKSAVGLGMLLSQGIGDTLRVSL
ECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIATVNALEERLEDITMPMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCE
SIIGCVVNGPGEALMSDLGITGGSNKSGFYEDGVRQRDRFDNNNVIDQLEAKIRARVSRT
EEEEEEECCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
DQSNIILKV
CCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA