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The map label for this gene is rlmN

Identifier: 119944920

GI number: 119944920

Start: 1454465

End: 1455583

Strand: Direct

Name: rlmN

Synonym: Ping_1165

Alternate gene names: 119944920

Gene position: 1454465-1455583 (Clockwise)

Preceding gene: 119944917

Following gene: 119944921

Centisome position: 31.9

GC content: 41.02

Gene sequence:

>1119_bases
ATGAATATAAAAAAAGTAAACCTACTGGATTTGAACCGCGAAGGTTTACGTGCGTTTTTTGTCGAATTGGGCGAAAAACC
CTTTCGTGCAGAGCAAGTGATGAAATGGATTTATCATTATGGCTGTGAAGATTTTGATCTGATGAGTAACGTTAATAAAA
AACTGCGCCAAAAATTAAAAGAATGCGCAGAAATTGTTGCACCAGAGATTAAAGTCGAACAACGCAGTAATGATGGAACG
ATAAAATGGGCTATGACGGTAGGCGATCAAGAAGTTGAGACTGTCTATATTCCAGAAAAAGATCGTGCGACTTTGTGCAT
TTCTTCACAGGTAGGTTGTGTATTAGCCTGTAATTTCTGCTCTACTGCCCAGCAGGGATTCAACCGTAATTTAAGCGTTA
GCGAAATTATTGGTCAAGTTTGGCGTGCGGCTAAAATTGTCGGTGTAACAGGGGAAAGCGGTAAGCGGCCCATTACTAAT
GTGGTCATGATGGGCATGGGAGAGCCGTTACTTAACCTTAATAATCTTATTCCTGCCATGGAATTGATGTTAGATGATTT
TGGTTATGCACTTTCAAAACGCCGTGTCACGGTATCAACATCAGGCGTTGTTCCTGCGCTTGATATTTTAGGCGATCGCA
TTGATGTTTCTTTAGCTATTTCATTGCATGCTGCCAACGATACATTGCGCTCTCAAATGATGCCCATTAACGATAAGTAC
AATATTGCTGATTTCTTAGCGGGTGTGAAACGTTATATCGCAAAATCAAAGGCTAACCGTGGTAAAGTATATATAGAGTA
TTTACTGCTGGACCATTTTAATGATTCGACTGATCAAGCTCATGAGTTGGCAATTTTACTTAAAGATACACCTTGTAAGA
TAAACCTGATTCCTTTTAACCCCTTCCCGGGTAATGATTACCAAAAACCAAGCAACAGCCGAGTGGATCGTTTTAATAAA
GTGTTAATGGAATATGGTTATACCGTTACCGTCCGTAAAACGCGCGGTGATGATATTGATGCTGCATGCGGGCAACTTGT
CGGAGATGTGATTGATCGGACTAAACGCACCATGAAAAAACGCCTGCAGGGTGAAAACATCGCGATTAGCGCGGGTTAA

Upstream 100 bases:

>100_bases
TTCCTAATTTTGATTATATCGGGACTGTTAACCTTTCATTGCAGAAAGTTCAACAGAGCCTGACAGTATTGAATAATTTA
TTGTTGTTAACGAGGCTTTC

Downstream 100 bases:

>100_bases
CTTTTTAGCCTGTCTTATTGTAAATGGCACAACTCAGCAGCTCAAAAAGAGTCTGGATTGTAGACATAAAGTTAAAAATA
GGCATTTCACATTAAAACTG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MNIKKVNLLDLNREGLRAFFVELGEKPFRAEQVMKWIYHYGCEDFDLMSNVNKKLRQKLKECAEIVAPEIKVEQRSNDGT
IKWAMTVGDQEVETVYIPEKDRATLCISSQVGCVLACNFCSTAQQGFNRNLSVSEIIGQVWRAAKIVGVTGESGKRPITN
VVMMGMGEPLLNLNNLIPAMELMLDDFGYALSKRRVTVSTSGVVPALDILGDRIDVSLAISLHAANDTLRSQMMPINDKY
NIADFLAGVKRYIAKSKANRGKVYIEYLLLDHFNDSTDQAHELAILLKDTPCKINLIPFNPFPGNDYQKPSNSRVDRFNK
VLMEYGYTVTVRKTRGDDIDAACGQLVGDVIDRTKRTMKKRLQGENIAISAG

Sequences:

>Translated_372_residues
MNIKKVNLLDLNREGLRAFFVELGEKPFRAEQVMKWIYHYGCEDFDLMSNVNKKLRQKLKECAEIVAPEIKVEQRSNDGT
IKWAMTVGDQEVETVYIPEKDRATLCISSQVGCVLACNFCSTAQQGFNRNLSVSEIIGQVWRAAKIVGVTGESGKRPITN
VVMMGMGEPLLNLNNLIPAMELMLDDFGYALSKRRVTVSTSGVVPALDILGDRIDVSLAISLHAANDTLRSQMMPINDKY
NIADFLAGVKRYIAKSKANRGKVYIEYLLLDHFNDSTDQAHELAILLKDTPCKINLIPFNPFPGNDYQKPSNSRVDRFNK
VLMEYGYTVTVRKTRGDDIDAACGQLVGDVIDRTKRTMKKRLQGENIAISAG
>Mature_372_residues
MNIKKVNLLDLNREGLRAFFVELGEKPFRAEQVMKWIYHYGCEDFDLMSNVNKKLRQKLKECAEIVAPEIKVEQRSNDGT
IKWAMTVGDQEVETVYIPEKDRATLCISSQVGCVLACNFCSTAQQGFNRNLSVSEIIGQVWRAAKIVGVTGESGKRPITN
VVMMGMGEPLLNLNNLIPAMELMLDDFGYALSKRRVTVSTSGVVPALDILGDRIDVSLAISLHAANDTLRSQMMPINDKY
NIADFLAGVKRYIAKSKANRGKVYIEYLLLDHFNDSTDQAHELAILLKDTPCKINLIPFNPFPGNDYQKPSNSRVDRFNK
VLMEYGYTVTVRKTRGDDIDAACGQLVGDVIDRTKRTMKKRLQGENIAISAG

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=367, Percent_Identity=74.6594005449591, Blast_Score=590, Evalue=1e-170,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_PSYIN (A1SU36)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942600.1
- ProteinModelPortal:   A1SU36
- SMR:   A1SU36
- STRING:   A1SU36
- GeneID:   4624554
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1165
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   A1SU36
- ProtClustDB:   PRK11194
- BioCyc:   PING357804:PING_1165-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 41652; Mature: 41652

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKKVNLLDLNREGLRAFFVELGEKPFRAEQVMKWIYHYGCEDFDLMSNVNKKLRQKLK
CCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ECAEIVAPEIKVEQRSNDGTIKWAMTVGDQEVETVYIPEKDRATLCISSQVGCVLACNFC
HHHHHHCCCEEEEECCCCCEEEEEEEECCCCEEEEECCCCCCEEEEEECCCCEEEEECHH
STAQQGFNRNLSVSEIIGQVWRAAKIVGVTGESGKRPITNVVMMGMGEPLLNLNNLIPAM
HHHHHHCCCCCCHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHH
ELMLDDFGYALSKRRVTVSTSGVVPALDILGDRIDVSLAISLHAANDTLRSQMMPINDKY
HHHHHHHHHHHHCCEEEEECCCCCHHHHHCCCCEEEEEEEEEECCCHHHHHHCCCCCCCC
NIADFLAGVKRYIAKSKANRGKVYIEYLLLDHFNDSTDQAHELAILLKDTPCKINLIPFN
CHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCHHEEEEEEECCCCEEEEEECC
PFPGNDYQKPSNSRVDRFNKVLMEYGYTVTVRKTRGDDIDAACGQLVGDVIDRTKRTMKK
CCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
RLQGENIAISAG
HHCCCCEEEECC
>Mature Secondary Structure
MNIKKVNLLDLNREGLRAFFVELGEKPFRAEQVMKWIYHYGCEDFDLMSNVNKKLRQKLK
CCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ECAEIVAPEIKVEQRSNDGTIKWAMTVGDQEVETVYIPEKDRATLCISSQVGCVLACNFC
HHHHHHCCCEEEEECCCCCEEEEEEEECCCCEEEEECCCCCCEEEEEECCCCEEEEECHH
STAQQGFNRNLSVSEIIGQVWRAAKIVGVTGESGKRPITNVVMMGMGEPLLNLNNLIPAM
HHHHHHCCCCCCHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHH
ELMLDDFGYALSKRRVTVSTSGVVPALDILGDRIDVSLAISLHAANDTLRSQMMPINDKY
HHHHHHHHHHHHCCEEEEECCCCCHHHHHCCCCEEEEEEEEEECCCHHHHHHCCCCCCCC
NIADFLAGVKRYIAKSKANRGKVYIEYLLLDHFNDSTDQAHELAILLKDTPCKINLIPFN
CHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCHHEEEEEEECCCCEEEEEECC
PFPGNDYQKPSNSRVDRFNKVLMEYGYTVTVRKTRGDDIDAACGQLVGDVIDRTKRTMKK
CCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
RLQGENIAISAG
HHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA