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The map label for this gene is pmbA [H]

Identifier: 119944888

GI number: 119944888

Start: 1419651

End: 1420976

Strand: Direct

Name: pmbA [H]

Synonym: Ping_1132

Alternate gene names: 119944888

Gene position: 1419651-1420976 (Clockwise)

Preceding gene: 119944886

Following gene: 119944894

Centisome position: 31.14

GC content: 41.93

Gene sequence:

>1326_bases
ATGATTGAACAGAAACAACAACTCGAAAATGCGGTTAATGAGGCATTAACATTTGCTCAGCAGCTTGGGGTTAATCAGGC
TGAAGTTGCACTCGGAAAGGCGACGGGTATTTCTGTTAATACACGCTTGGGAGAGGTCGAAAATATTGAGTTTAATCATG
ATGGTGCGATGGGAATTAGCGTGTATGTAAACCAATGTAAGGGAAGTGCATCAACGGCTGATTTAAGTTTAGAAGCCATT
CACTCAAGCGTTAAAGCTGCGGTAGATATTGCCAAGTTTACCAGTAAAGACCCGTTCTCCGGTTTATTGGATCCTGAATT
TTTAGAAAAAAATCCACTTGATTTAGATCTTTATCATCCCTGCAACTATACGACAGAAGAGTTTTTGGCAGTTGCCAAAG
AGTGTGAGGAGATCGCATTATCCAGTGACTCGCGTATTGTGAACTCCGATGGGGCCGCATTTAACAGTCATTCAAGTCTC
AAAGTATATGGTAATACCAATGGTTTCCTGCACAGTTATGCAACCAGTCGTCATAGTTTAAGTTGTGTGTTAATTGCAAA
GCAGCAACAGGAAATGCAACGAAATTACAGTTACACTAGCGGGCGGGACGTTAATGACCTATTGAGCGCAAAATGGGTGG
CAGAAGATGCTGCAAAGCGTACTTTAGATCGTTTAGGTGCAACTAAAATAAAAACCCAAGAAAGCGCGGTTATTTTTGCC
CCAGAAGTTGCTACCGGCTTATTTGGGCATCTCGTTGGTGCTATCAGTGGGATGAGTTTGTATAAAAAATCAAGTTTTTT
ACTGGATAGTTTAGGGACGAAAATATTTCCTGAATGGATGCAAATTTCGGAGCACCCGCATATTATGAAAGGTCTAGCCA
GTGCGCCCTATGACCAGGAAGGGTGCAAAACGATTCAGCGAAATATTGTTGAAAGTGGCGTACTGCAGACCTATTTATTA
CCCGGCTATGCTTCTCGCAAAATGAAAATGGAAAGTACAGGCCATGCCGGAGGCATTCATAACTGGCATGTTAAAACAGG
TGATTTAGATTTGCCCGGATTATTTAAAGAAATGGGCACTGGGTTGTTTGTTACCGAATTAATGGGGCAAGGTGTGAATT
TGGTGAGTGGAGATTATTCTCGCGGAGCGAGCGGTTTTTGGATTGAGAATGGGGTGATTGTTTGTCCTGTACATGAAATT
ACCATTGCGGGAAATTTAAAAGAGATGTTTATGGGGATTGAAGCGATCGGCAATGATATTGATCCGCGCAGCAGCCTTAA
AACAGGCTCTATTTTAATTAACAAAATGAAAATAGCCGGGCAATAA

Upstream 100 bases:

>100_bases
ATTTTTATGGCGTATTCTTATTAACCAGGAATTTGTTTTTTAACTGATTCGGATACTTTTCACGATCAAATTTTATTTTA
TTCTAAGTGGTGTATTAATA

Downstream 100 bases:

>100_bases
TAGTTTGGGTATAAAAAATAAGCCTATTTTCGAATAGGCTTATTTTATTTAAACATTATTTCTTATGCACAATAAATGCC
TGCGGGTAACCATTTTTTAT

Product: peptidase U62, modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MIEQKQQLENAVNEALTFAQQLGVNQAEVALGKATGISVNTRLGEVENIEFNHDGAMGISVYVNQCKGSASTADLSLEAI
HSSVKAAVDIAKFTSKDPFSGLLDPEFLEKNPLDLDLYHPCNYTTEEFLAVAKECEEIALSSDSRIVNSDGAAFNSHSSL
KVYGNTNGFLHSYATSRHSLSCVLIAKQQQEMQRNYSYTSGRDVNDLLSAKWVAEDAAKRTLDRLGATKIKTQESAVIFA
PEVATGLFGHLVGAISGMSLYKKSSFLLDSLGTKIFPEWMQISEHPHIMKGLASAPYDQEGCKTIQRNIVESGVLQTYLL
PGYASRKMKMESTGHAGGIHNWHVKTGDLDLPGLFKEMGTGLFVTELMGQGVNLVSGDYSRGASGFWIENGVIVCPVHEI
TIAGNLKEMFMGIEAIGNDIDPRSSLKTGSILINKMKIAGQ

Sequences:

>Translated_441_residues
MIEQKQQLENAVNEALTFAQQLGVNQAEVALGKATGISVNTRLGEVENIEFNHDGAMGISVYVNQCKGSASTADLSLEAI
HSSVKAAVDIAKFTSKDPFSGLLDPEFLEKNPLDLDLYHPCNYTTEEFLAVAKECEEIALSSDSRIVNSDGAAFNSHSSL
KVYGNTNGFLHSYATSRHSLSCVLIAKQQQEMQRNYSYTSGRDVNDLLSAKWVAEDAAKRTLDRLGATKIKTQESAVIFA
PEVATGLFGHLVGAISGMSLYKKSSFLLDSLGTKIFPEWMQISEHPHIMKGLASAPYDQEGCKTIQRNIVESGVLQTYLL
PGYASRKMKMESTGHAGGIHNWHVKTGDLDLPGLFKEMGTGLFVTELMGQGVNLVSGDYSRGASGFWIENGVIVCPVHEI
TIAGNLKEMFMGIEAIGNDIDPRSSLKTGSILINKMKIAGQ
>Mature_441_residues
MIEQKQQLENAVNEALTFAQQLGVNQAEVALGKATGISVNTRLGEVENIEFNHDGAMGISVYVNQCKGSASTADLSLEAI
HSSVKAAVDIAKFTSKDPFSGLLDPEFLEKNPLDLDLYHPCNYTTEEFLAVAKECEEIALSSDSRIVNSDGAAFNSHSSL
KVYGNTNGFLHSYATSRHSLSCVLIAKQQQEMQRNYSYTSGRDVNDLLSAKWVAEDAAKRTLDRLGATKIKTQESAVIFA
PEVATGLFGHLVGAISGMSLYKKSSFLLDSLGTKIFPEWMQISEHPHIMKGLASAPYDQEGCKTIQRNIVESGVLQTYLL
PGYASRKMKMESTGHAGGIHNWHVKTGDLDLPGLFKEMGTGLFVTELMGQGVNLVSGDYSRGASGFWIENGVIVCPVHEI
TIAGNLKEMFMGIEAIGNDIDPRSSLKTGSILINKMKIAGQ

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=438, Percent_Identity=54.7945205479452, Blast_Score=510, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47838; Mature: 47838

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEQKQQLENAVNEALTFAQQLGVNQAEVALGKATGISVNTRLGEVENIEFNHDGAMGIS
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHEECCCCCEEEECCCCCEEEEEECCCCCEEEE
VYVNQCKGSASTADLSLEAIHSSVKAAVDIAKFTSKDPFSGLLDPEFLEKNPLDLDLYHP
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEEECC
CNYTTEEFLAVAKECEEIALSSDSRIVNSDGAAFNSHSSLKVYGNTNGFLHSYATSRHSL
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEECCCCEEEHHHCCCCCE
SCVLIAKQQQEMQRNYSYTSGRDVNDLLSAKWVAEDAAKRTLDRLGATKIKTQESAVIFA
EEEEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEC
PEVATGLFGHLVGAISGMSLYKKSSFLLDSLGTKIFPEWMQISEHPHIMKGLASAPYDQE
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHCHHHHHHCCCCHHHHHHHCCCCCHH
GCKTIQRNIVESGVLQTYLLPGYASRKMKMESTGHAGGIHNWHVKTGDLDLPGLFKEMGT
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCCHHHHHHCC
GLFVTELMGQGVNLVSGDYSRGASGFWIENGVIVCPVHEITIAGNLKEMFMGIEAIGNDI
CEEHHHHHCCCCEEECCCCCCCCCCEEEECCEEEEECEEEEEECCHHHHHHHHHHHCCCC
DPRSSLKTGSILINKMKIAGQ
CCHHCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MIEQKQQLENAVNEALTFAQQLGVNQAEVALGKATGISVNTRLGEVENIEFNHDGAMGIS
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHEECCCCCEEEECCCCCEEEEEECCCCCEEEE
VYVNQCKGSASTADLSLEAIHSSVKAAVDIAKFTSKDPFSGLLDPEFLEKNPLDLDLYHP
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEEECC
CNYTTEEFLAVAKECEEIALSSDSRIVNSDGAAFNSHSSLKVYGNTNGFLHSYATSRHSL
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEECCCCEEEHHHCCCCCE
SCVLIAKQQQEMQRNYSYTSGRDVNDLLSAKWVAEDAAKRTLDRLGATKIKTQESAVIFA
EEEEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEC
PEVATGLFGHLVGAISGMSLYKKSSFLLDSLGTKIFPEWMQISEHPHIMKGLASAPYDQE
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHCHHHHHHCCCCHHHHHHHCCCCCHH
GCKTIQRNIVESGVLQTYLLPGYASRKMKMESTGHAGGIHNWHVKTGDLDLPGLFKEMGT
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCCHHHHHHCC
GLFVTELMGQGVNLVSGDYSRGASGFWIENGVIVCPVHEITIAGNLKEMFMGIEAIGNDI
CEEHHHHHCCCCEEECCCCCCCCCCEEEECCEEEEECEEEEEECCHHHHHHHHHHHCCCC
DPRSSLKTGSILINKMKIAGQ
CCHHCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]