Click here to switch to the map view.

The map label for this gene is iciA [H]

Identifier: 119944770

GI number: 119944770

Start: 1273276

End: 1274172

Strand: Direct

Name: iciA [H]

Synonym: Ping_1012

Alternate gene names: 119944770

Gene position: 1273276-1274172 (Clockwise)

Preceding gene: 119944768

Following gene: 119944773

Centisome position: 27.93

GC content: 38.68

Gene sequence:

>897_bases
ATGATTAATTTAGATTACAAACTATTATCTGCATTAAATGAAATTGTTGAACAGCAAGGTTTTGAACTTGCTGCTAAAAA
ATTATTTATCAGCCAACCTGCGATTTCATTGCGCCTGAAAAATTTGGAAGAGAAGGTCGGTCAACCGCTCGTCATTCGTG
GACATCCCATTAGGGTTACCCCCGCCGGTGAAAAACTCTTAAGTCATTTTAAAATGGTGCAGCAGTTAGAGAATGATTTA
ATGACAAATTTATTTCCCACTATATCGAGTCACCCGGTAAAAATATCTTTAGCGGTGAATGCAGACAGTATTGCAACTTG
GTTTCTTGATGCGATGGCACCGATTTTAAAAAAATATCTTATTGAAATGAATTTAATCATTGCTAATGGGGTAGAAACCC
TGAATAAGTTACGCTCTGGGCAAGCGGTTGGTGCCGTGATGAATATTGAATCGCCTCTGCCCGGTTATCGCTCTTTTAAA
CTCGGGGGAATGAATTATATATTAGTTGCCTCAAAGGATTTCCAAAAAAAATATTTTAGTCAGGGGGTGAATAAGGAAAG
CTTAAAAATGGCCCCGAGTATTAACTATGATTCTAAAGATAATATGCACCAGAATTTTATTAGCAAACACTTTGACTTGA
ATGCCAGCGAATACTCCAGTCATACAATAAGCTCCTCAGAAGCCTTTGTCGATTTGGCAAAACGCAGTTTAGTTTGCTGT
TTAATTCCGCAATTGCAGATAGAAAGGGAGCTGGCCGAGGGGGAACTGATTAATCTCTGTCCGAATTATCGGTTAAGCGA
AACACTTTACTGGCACAGTTGGGTATTAGTGAAAGGGTTAAATAAACAAATCTCGCAGGAAATCGTGGCTTACGGGCGAC
AAGTTTTAGAAAACTAA

Upstream 100 bases:

>100_bases
TTCATTATTCCGAGTTGAGGTTATTTAAGTGAGGCAGGATTTTGCACGTTTTAGCCACATAAGTATAATGAATAATATTT
ATGGTATATAAATTTGGCTT

Downstream 100 bases:

>100_bases
TACTAGGCTGCAGAGGGTTATTTGAAGTAGATCGTAGGGTGCGCAGCGATTGCGGTGCACAGCTACCGGTAACATAATAC
CGATTCCATTAATTAACTCC

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MINLDYKLLSALNEIVEQQGFELAAKKLFISQPAISLRLKNLEEKVGQPLVIRGHPIRVTPAGEKLLSHFKMVQQLENDL
MTNLFPTISSHPVKISLAVNADSIATWFLDAMAPILKKYLIEMNLIIANGVETLNKLRSGQAVGAVMNIESPLPGYRSFK
LGGMNYILVASKDFQKKYFSQGVNKESLKMAPSINYDSKDNMHQNFISKHFDLNASEYSSHTISSSEAFVDLAKRSLVCC
LIPQLQIERELAEGELINLCPNYRLSETLYWHSWVLVKGLNKQISQEIVAYGRQVLEN

Sequences:

>Translated_298_residues
MINLDYKLLSALNEIVEQQGFELAAKKLFISQPAISLRLKNLEEKVGQPLVIRGHPIRVTPAGEKLLSHFKMVQQLENDL
MTNLFPTISSHPVKISLAVNADSIATWFLDAMAPILKKYLIEMNLIIANGVETLNKLRSGQAVGAVMNIESPLPGYRSFK
LGGMNYILVASKDFQKKYFSQGVNKESLKMAPSINYDSKDNMHQNFISKHFDLNASEYSSHTISSSEAFVDLAKRSLVCC
LIPQLQIERELAEGELINLCPNYRLSETLYWHSWVLVKGLNKQISQEIVAYGRQVLEN
>Mature_298_residues
MINLDYKLLSALNEIVEQQGFELAAKKLFISQPAISLRLKNLEEKVGQPLVIRGHPIRVTPAGEKLLSHFKMVQQLENDL
MTNLFPTISSHPVKISLAVNADSIATWFLDAMAPILKKYLIEMNLIIANGVETLNKLRSGQAVGAVMNIESPLPGYRSFK
LGGMNYILVASKDFQKKYFSQGVNKESLKMAPSINYDSKDNMHQNFISKHFDLNASEYSSHTISSSEAFVDLAKRSLVCC
LIPQLQIERELAEGELINLCPNYRLSETLYWHSWVLVKGLNKQISQEIVAYGRQVLEN

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=298, Percent_Identity=42.2818791946309, Blast_Score=253, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33570; Mature: 33570

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLDYKLLSALNEIVEQQGFELAAKKLFISQPAISLRLKNLEEKVGQPLVIRGHPIRVT
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCEEEC
PAGEKLLSHFKMVQQLENDLMTNLFPTISSHPVKISLAVNADSIATWFLDAMAPILKKYL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHH
IEMNLIIANGVETLNKLRSGQAVGAVMNIESPLPGYRSFKLGGMNYILVASKDFQKKYFS
HHHHHHEECCHHHHHHHHCCCHHEEEEECCCCCCCCCEEEECCCEEEEEECCHHHHHHHH
QGVNKESLKMAPSINYDSKDNMHQNFISKHFDLNASEYSSHTISSSEAFVDLAKRSLVCC
CCCCHHHHEECCCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHH
LIPQLQIERELAEGELINLCPNYRLSETLYWHSWVLVKGLNKQISQEIVAYGRQVLEN
HCCHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MINLDYKLLSALNEIVEQQGFELAAKKLFISQPAISLRLKNLEEKVGQPLVIRGHPIRVT
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCEEEC
PAGEKLLSHFKMVQQLENDLMTNLFPTISSHPVKISLAVNADSIATWFLDAMAPILKKYL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHH
IEMNLIIANGVETLNKLRSGQAVGAVMNIESPLPGYRSFKLGGMNYILVASKDFQKKYFS
HHHHHHEECCHHHHHHHHCCCHHEEEEECCCCCCCCCEEEECCCEEEEEECCHHHHHHHH
QGVNKESLKMAPSINYDSKDNMHQNFISKHFDLNASEYSSHTISSSEAFVDLAKRSLVCC
CCCCHHHHEECCCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHH
LIPQLQIERELAEGELINLCPNYRLSETLYWHSWVLVKGLNKQISQEIVAYGRQVLEN
HCCHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9286976 [H]